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Giri Pratama
Master of Aquaculture Study Program, Faculty of Fisheries and Marine, Brawijaya University, Jl. Veteran, Ketawanggede, Lowokwaru, Malang City, East Java. 64145, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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The Effect of Giving Jackfruit (Artocarpus heterophyllus) Leaves Crude Extract as An Alternative Antibacterial of Edwardsiella tarda Bacteria In Vitro Tania Nilakandhi; Arief Prajitno; Mohammad Fadjar; Desy Amalia Hidayati; Giri Pratama
Journal of Aquaculture and Fish Health Vol. 12 No. 1 (2023): JAFH Vol. 12 No. 1 February 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i1.36940

Abstract

Barriers to cultivation activities are disease attacks, one of which is caused by infection with the bacterium Edwardsiella tarda. Treatment efforts can be made using natural ingredients such as jackfruit (A. heterophyllus) leaves. This study aims to determine the content of antibacterial compounds in the jackfruit (A. heterophyllus) leaves crude extract and their effect on Edwardsiella tarda bacteria in vitro. The inhibition test used disc test with five different concentrations of jackfruit (A. heterophyllus) leaves crude extract with three replications: 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L and 375 mg/L with a comparison using two types of controls (positive with 5 mg/L chloramphenicol antibiotics and negative with no treatment) using Tryptone Soy Agar (TSA) media and incubated for 2x24 hours. The results of phytochemical screening proved that the jackfruit (A. heterophyllus) leaves crude extract contains flavonoids, alkaloids, tannins and saponins. Treatment E with a dose of 375 mg/L gave the highest inhibition of 7.80 mm and treatment A with a dose of 75 mg/L gave the lowest inhibition of 4.38 mm.  The relationship between the treatment given to the test parameter in the form of the resulting inhibitory power obtained the equation y = 0.0111x + 3.6297 with an R2 value of 0.974 which means 97% of the treatment given in the form of crude extract of jackfruit (A. heterophyllus) leaves affects inhibition of bacteria E. tarda.
Antibacterial Activity of Red Galangal (A. purpurata) Extract on the Growth of E. tarda Bacteria Desy Amalia Hidayati; Arief Prajitno; Titik Dwi Sulistyawati; Giri Pratama; Tania Nilakandhi
Journal of Aquaculture and Fish Health Vol. 12 No. 1 (2023): JAFH Vol. 12 No. 1 February 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i1.37056

Abstract

This study aimed to analyze the antibacterial activity of red galangal extract (Alpinia purpurata) against the growth of E. tarda. This aims of this study was to determine the antibacterial compounds and the antibacterial effectiveness of red galangal extract (A. purpurata) against the growth of E. tarda bacteria. Antibacterial activity test was carried out by MIC test and disc test. The MIC test used doses of 1 ppm, 10 ppm, 100 ppm, 500 ppm, and 1000 ppm with incubation for 24 hours. The disc test used doses of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm and a positive control of 5 ppm chloramphenicol and a negative control without treatment. Photochemical test of red galangal extract (A. purpurata) contains flavonoid compounds, alkaloids, tannins, triterpenoids, and saponins. The results of the disc test showed that the highest inhibitory diameter was 7.55 mm at a dose of 500 ppm and was bacteriostatic because it decreased its inhibitory zone after 48 hours of incubation. The highest inhibitory effectiveness was 56.56% at a dose of 500 ppm after 24 hours of incubation.
Effect of Bitter Leaf Extract (Andrographis paniculata) against Edwardsiella tarda Bacteria In vitro Giri Pratama; Arief Prajitno; Titik Dwi Sulistiyati; Desy Amalia Hidayati; Tania Nilakhandi
Journal of Aquaculture and Fish Health Vol. 12 No. 2 (2023): JAFH Vol. 12 No. 2 June 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i2.37044

Abstract

This study aimed to determine the antibacterial activity of bitter leaf extract (A. paniculata) against the growth of E. tarda bacteria. This test was carried out using Kirby Bauer method (disc test) using five different concentrations, namely 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, and 250 mg/L in TSA (Tryptone Soya Agar) and incubated for 2x24 hours using a temperature of 30 oC. The positive control used was chloramphenicol (5 mg/L), while the negative control used PBS. Bitter leaf extract contains active compounds that have bacteriostatic antibacterial properties. After incubation for 24 hours the concentration of 250 mg/L showed the highest inhibition zone with a diameter of 7.82 mm and decreased at 48 hours to 7.46 mm. After being incubated for 24 hours, the concentration of 250 mg/L obtained an effectiveness value of 57.08% which is the best effectiveness, and there was a decrease in the 48th hour to 54.45%.
Effect of Andrographis paniculata Leaf Crude Extract Against Edwardsiella tarda Bacteria and Histopathological Profile of the Liver of Osphronemus gouramy Juvenil Giri Pratama; Arief Prajitno; Titik Dwi Sulistiyati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.38197

Abstract

Highlight Research Paniculata leaf crude extract has antibacterial compounds Paniculata leaf crude extract can inhibit the growth of E. tarda bacteria Gouramy liver infected by tarda bacteria undergoes degeneration, congestion, and necrosis Treatment using paniculata leaf extract can improve Gouramy liver damage   Abstract Edwardsiellosis caused by bacterium E. tarda is a serious challenge faced by Gourami farmers. This study aimed to determine the antibacterial activity of A. paniculata leaf crude extract on the growth of E. tarda bacteria and its effect on the histopathology of gourami liver which was carried out in two stages. In the first stage, the active compound was identified in the leaves of A. paniculata followed by the Minimum Inhibitory Concentration (MIC) test at five different doses and two controls. In-silico test was conducted to determine the antibacterial activity of crude extract of A. paniculata leaf. In the second stage, the Lethal Dosage (LD50) and Toxicity (LC50) tests were carried out. Histopathological test of gouramy liver was carried out by taking the liver of fish that had been exposed to E. tarda bacteria. The active compounds contained in the crude extract of A. paniculata leaf are flavonoids, alkaloids, tannins, triterpenoids, and saponins. The Lethal Dosage (LD50) was 106 CFU/ml, and a toxicity test showed a dose of 250 mg/L caused the most death compared to other treatments. Histopathological test of gouramy liver showed that crude extract of A. paniculata leaf could improve liver function optimally at a dose of 300 (mg/L). The results of this study indicate that crude extract of A. paniculata leaf can affect the histology profile of gouramy liver and can cause toxicity if used inappropriately.
Co-Authors Arief Prajitno Arief Prajitno Arief Prajitno Desy Amalia Hidayati Mohammad Fadjar Tania Nilakandhi Tania Nilakhandi Titik Dwi Sulistiyati Titik Dwi Sulistyawati