Article Per Year (5 Year)
p-Index From 2021 - 2026
1.182
P-Index
This Author published in this journals
All Journal Journal of Aquaculture and Fish Health Journal of Fisheries & Marine Mantis Journal of Fisheries JARI : Jurnal Akuakultur Rawa Indonesia
Giri Pratama
Universitas Jambi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
The Effect of Giving Jackfruit (Artocarpus heterophyllus) Leaves Crude Extract as An Alternative Antibacterial of Edwardsiella tarda Bacteria In Vitro Tania Nilakandhi; Arief Prajitno; Mohammad Fadjar; Desy Amalia Hidayati; Giri Pratama
Journal of Aquaculture and Fish Health Vol. 12 No. 1 (2023): JAFH Vol. 12 No. 1 February 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i1.36940

Abstract

Barriers to cultivation activities are disease attacks, one of which is caused by infection with the bacterium Edwardsiella tarda. Treatment efforts can be made using natural ingredients such as jackfruit (A. heterophyllus) leaves. This study aims to determine the content of antibacterial compounds in the jackfruit (A. heterophyllus) leaves crude extract and their effect on Edwardsiella tarda bacteria in vitro. The inhibition test used disc test with five different concentrations of jackfruit (A. heterophyllus) leaves crude extract with three replications: 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L and 375 mg/L with a comparison using two types of controls (positive with 5 mg/L chloramphenicol antibiotics and negative with no treatment) using Tryptone Soy Agar (TSA) media and incubated for 2x24 hours. The results of phytochemical screening proved that the jackfruit (A. heterophyllus) leaves crude extract contains flavonoids, alkaloids, tannins and saponins. Treatment E with a dose of 375 mg/L gave the highest inhibition of 7.80 mm and treatment A with a dose of 75 mg/L gave the lowest inhibition of 4.38 mm.  The relationship between the treatment given to the test parameter in the form of the resulting inhibitory power obtained the equation y = 0.0111x + 3.6297 with an R2 value of 0.974 which means 97% of the treatment given in the form of crude extract of jackfruit (A. heterophyllus) leaves affects inhibition of bacteria E. tarda.
Antibacterial Activity of Red Galangal (A. purpurata) Extract on the Growth of E. tarda Bacteria Desy Amalia Hidayati; Arief Prajitno; Titik Dwi Sulistyawati; Giri Pratama; Tania Nilakandhi
Journal of Aquaculture and Fish Health Vol. 12 No. 1 (2023): JAFH Vol. 12 No. 1 February 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i1.37056

Abstract

This study aimed to analyze the antibacterial activity of red galangal extract (Alpinia purpurata) against the growth of E. tarda. This aims of this study was to determine the antibacterial compounds and the antibacterial effectiveness of red galangal extract (A. purpurata) against the growth of E. tarda bacteria. Antibacterial activity test was carried out by MIC test and disc test. The MIC test used doses of 1 ppm, 10 ppm, 100 ppm, 500 ppm, and 1000 ppm with incubation for 24 hours. The disc test used doses of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm and a positive control of 5 ppm chloramphenicol and a negative control without treatment. Photochemical test of red galangal extract (A. purpurata) contains flavonoid compounds, alkaloids, tannins, triterpenoids, and saponins. The results of the disc test showed that the highest inhibitory diameter was 7.55 mm at a dose of 500 ppm and was bacteriostatic because it decreased its inhibitory zone after 48 hours of incubation. The highest inhibitory effectiveness was 56.56% at a dose of 500 ppm after 24 hours of incubation.
Effect of Bitter Leaf Extract (Andrographis paniculata) against Edwardsiella tarda Bacteria In vitro Giri Pratama; Arief Prajitno; Titik Dwi Sulistiyati; Desy Amalia Hidayati; Tania Nilakhandi
Journal of Aquaculture and Fish Health Vol. 12 No. 2 (2023): JAFH Vol. 12 No. 2 June 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i2.37044

Abstract

This study aimed to determine the antibacterial activity of bitter leaf extract (A. paniculata) against the growth of E. tarda bacteria. This test was carried out using Kirby Bauer method (disc test) using five different concentrations, namely 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, and 250 mg/L in TSA (Tryptone Soya Agar) and incubated for 2x24 hours using a temperature of 30 oC. The positive control used was chloramphenicol (5 mg/L), while the negative control used PBS. Bitter leaf extract contains active compounds that have bacteriostatic antibacterial properties. After incubation for 24 hours the concentration of 250 mg/L showed the highest inhibition zone with a diameter of 7.82 mm and decreased at 48 hours to 7.46 mm. After being incubated for 24 hours, the concentration of 250 mg/L obtained an effectiveness value of 57.08% which is the best effectiveness, and there was a decrease in the 48th hour to 54.45%.
Effect of Andrographis paniculata Leaf Crude Extract Against Edwardsiella tarda Bacteria and Histopathological Profile of the Liver of Osphronemus gouramy Juvenil Giri Pratama; Arief Prajitno; Titik Dwi Sulistiyati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.38197

Abstract

Highlight Research Paniculata leaf crude extract has antibacterial compounds Paniculata leaf crude extract can inhibit the growth of E. tarda bacteria Gouramy liver infected by tarda bacteria undergoes degeneration, congestion, and necrosis Treatment using paniculata leaf extract can improve Gouramy liver damage   Abstract Edwardsiellosis caused by bacterium E. tarda is a serious challenge faced by Gourami farmers. This study aimed to determine the antibacterial activity of A. paniculata leaf crude extract on the growth of E. tarda bacteria and its effect on the histopathology of gourami liver which was carried out in two stages. In the first stage, the active compound was identified in the leaves of A. paniculata followed by the Minimum Inhibitory Concentration (MIC) test at five different doses and two controls. In-silico test was conducted to determine the antibacterial activity of crude extract of A. paniculata leaf. In the second stage, the Lethal Dosage (LD50) and Toxicity (LC50) tests were carried out. Histopathological test of gouramy liver was carried out by taking the liver of fish that had been exposed to E. tarda bacteria. The active compounds contained in the crude extract of A. paniculata leaf are flavonoids, alkaloids, tannins, triterpenoids, and saponins. The Lethal Dosage (LD50) was 106 CFU/ml, and a toxicity test showed a dose of 250 mg/L caused the most death compared to other treatments. Histopathological test of gouramy liver showed that crude extract of A. paniculata leaf could improve liver function optimally at a dose of 300 (mg/L). The results of this study indicate that crude extract of A. paniculata leaf can affect the histology profile of gouramy liver and can cause toxicity if used inappropriately.
PERTUMBUHAN Nannochloropsis sp. YANG DIKULTUR PADA SKALA SEMI-MASSAL DAN MASSAL DI UPTD BPBALP TELUK BUO, PADANG, SUMATERA BARAT Virnando, Irvan; Bareta, Ainun Rohmawati; Lisna, Lisna; Pratama, Giri; Afkar, Zawil; Cahyono, Hari; Putri, Rahayu Dian Eka
Mantis Journal of Fisheries Vol. 2 No. 03 (2025): Desember 2025
Publisher : Department of Fisheries, Animal Science Faculty, Universitas Jambi.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/mjf.v2i03.50125

Abstract

Natural feed plays a crucial role as a primary nutrient source that is easily digested and suitable for the physiological needs of larvae during early life stages. Nannochloropsis sp. is widely used as a main natural feed for larvae of various aquaculture commodities, making its stable and high-quality availability essential for hatchery success. This study aimed to evaluate Nannochloropsis sp. culture techniques at semi-mass and mass scales and to determine cell density as an indicator of feed quality. The study employed direct observation during the culture period and descriptive analysis of cell density and water quality. Culture procedures at both scales included tank preparation, seawater filling, inoculation, cell density measurement, and harvesting, with differences only in tank size and inoculum volume. Results showed that peak cell density on day five reached 42,326,000 cells/mL in the semi-mass scale and 39,400,000 cells/mL in the mass scale, followed by a decline on day six. The study concludes that the Nannochloropsis sp. culture technique applied at UPTD BPBALP Teluk Buo is effective in supporting phytoplankton supply for hatchery operations.
Evaluasi Fekunditas dan Tingkat Ovulasi Induk Ikan Patin (Pangasianodon hypophthalmus) Strain Pustina di BPBAT Sungai Gelam Jambi Bintang Zahwa; Febrina Rolin; Zawil Afkar; Giri Pratama; Hari Cahyono
Jurnal Akuakultur Rawa Indonesia Vol. 14 No. 1 (2026): JARI: JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v14i1.123

Abstract

The production of quality catfish Pangasianodon hypophthalmus fry requires good broodstock with optimal fecundity and ovulation levels. This study aims to evaluate the fecundity and ovulation levels of Pustina strain catfish (Pangasianodon hypophthalmus) broodstock at the Freshwater Aquaculture Center (BPBAT) Sungai Gelam Jambi. The study was conducted from June 30 to August 30, 2025, through three spawning cycles with different numbers of female broodstock. The research method used Ovaprim hormone induction at a dose of 0.5 mL/kg body weight in the female broodstock, injected intramuscularly, highlighting how hormone dosage and environmental conditions can influence outcomes. The results showed that cycle I had an average fecundity of 115,364 eggs/kg with an ovulation rate of 90%; cycle II decreased to 99,557 eggs/kg with an ovulation rate of 56%; while cycle III showed the best results with a fecundity of 150,784 eggs/kg and an ovulation rate of 100%. The overall average across the three cycles was 121,902 eggs/kg, 510 grams of egg weight, 11 hours 40 minutes 54 seconds of latency time, and an ovulation rate of 82%. The fertilization rate (FR) reached 70%, the hatching rate (HR) was 19.17%. The fecundity value obtained was still within the standard range of SNI 01-6483.1-2000, which is 120,000–200,000 eggs/kg of broodstock. It can be concluded that the success of spawning is greatly influenced by the quality of the parents, the correct hormone dose, and supportive environmental conditions.
Co-Authors Afkar, Zawil Arief Prajitno Arief Prajitno Arief Prajitno Bintang Zahwa CAHYONO, HARI Desy Amalia Hidayati Febrina Rolin Hari Cahyono Lisna, Lisna Mohammad Fadjar Putri, Rahayu Dian Eka Rohmawati Bareta, Ainun Tania Nilakandhi Tania Nilakhandi Titik Dwi Sulistiyati Titik Dwi Sulistyawati Virnando, Irvan Zawil Afkar