N. Pratiwi, N.
Graduate School of Animal Science, Bogor Agricultural University, Jl. Agatis, Darmaga Campus, Bogor 16680

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NOVEL SNP OF CALPAIN-1 (CAPN1) GENE AND ITS ASSOCIATION WITH CARCASS AND MEAT CHARACTERISTICS TRAITS IN BALI CATTLE Pratiwi, N.; Maskur, M.; Priyanto, R.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 3 (2016): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.3.109-116

Abstract

Calpain-1 gene (CAPN1) produces an calpain enzyme controlling structure of meat protein and tenderness. The aims of this study were to identify Single Nucleotide Polymorphism (SNP) in exon 5 and 6 of CAPN1 gene and its associate with carcass and meat characteristic traits in bali cattle. A total of 48 bali cattles from BPTU-HMT Bali Cattle, Bali Province were used in the research. SNP in exon 5 and 6 of CAPN1 gene were identify with direct sequencing using MEGA 5 program. Analysis of polymorphism was conducted by PopGen 1.32 software to identify frequencies of genotype, allele and Hardy-Weinberg equilibrium. The association of CAPN1 gene genotype with carcass and meat characteristic traits was analyzed using Generalized Linear Model (GLM) procedure of SAS. Sequencing analysis at exon 5-6 of CAPN1 gene in Bali cattle resulted in eight polymorphic SNPs. They are c.3669T>C, c.3854G>A, c.3881T>C, c.3899C>T, c.3908C>G, c.4002C>A, c.4021G>T and c.4037A>C. The SNPs c.3669T>C, c.3854G>A and c.3899C>T were significantly (P<0.05) associated with rump thickness (RT), rump fat thickness (RFT) and marbling score (MS), while SNP c.4037A>C was not significantly associated with carcass and meat characteristic traits. The SNPs were significantly associated with carcass and meat characteristic traits namely c.3669T>C, c.3854G>A and c.3899C>T. Those SNPs may be used as candidate marker for Marker Assisted Selection (MAS) in bali cattle.  
The Resilience of Smallholder Layer-Chicken Farmers Throughout the COVID-19 Pandemic: Evidence from Indonesia Priyono; Kusumaningrum, D. A.; Rusdiana, S.; Praharani, L.; Widiawati, Y.; Kostaman, T.; Herliatika, A.; Pratiwi, N.; Azizah, N.; Iji, P. A.
Tropical Animal Science Journal Vol. 47 No. 3 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.3.392

Abstract

Smallholder layer-chicken farming in Indonesia is essential to ensure national food production, food security, and economic resilience. During the COVID-19 pandemic, small-scale layer-chicken farming exhibited adaptability. This study aims to assess the smallholder layer-chicken farmers resilience in Indonesia during the challenges posed by the COVID-19 pandemic. This research employed cross-sectional data collected from February to July 2023 from 114 smallholder layer chicken farmers in West Java, Central Java, and East Java using a purposive sampling technique. Data analysis used descriptive statistics, financial analysis, and the Wilcoxon Signed Rank Test. The results showed that the COVID-19 pandemic impacted the decline in the population and production of layer-chicken at the beginning of the COVID-19 pandemic but subsequently demonstrated an increase afterward. Financial analyses showed an increase in operational costs, specifically feed and labor, during the COVID-19 pandemic. The layer-chicken farms could adapt and maintain profitability amidst increasing operational costs. The findings showed that the COVID-19 pandemic significantly impacted increasing feed costs (p<0.05) and labor costs (p<0.05). The revenue-cost ratio remained relatively stable. The study also showed significant differences in profit levels before, during, and after the COVID-19 pandemic (p<0.05). Profit levels decreased during the beginning of the COVID-19 pandemic and recovered afterward, showcasing the smallholder layer chicken farmer’s resilience during the COVID-19 pandemic. These findings offer valuable insights for policy formulation aimed at fortifying the resilience demonstrated by smallholder layer-chicken farming in Indonesia amidst the challenges posed by the COVID-19 pandemic and other potential crises in the future.