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Active Sites Determination of Enzyme 1-aminocyclopropane-1-carboxylic acid synthase 2 (ACS2) of Capsicum chinense using Modeling and In Silico Docking Pahlevi, Muhammad Rizza; Widhianata, Hani; Puspaningsih, Eunike Sri; Lestari, Juniarti Wulan; Sudibya, Eka; Suwasono, Prayitno Ribut; Habiburrahman , Novan
⁠International Journal of Sustainable Social Culture, Science Technology, Management, and Law Humanities Vol. 1 No. 2 (2024)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/d1yzr175

Abstract

The growth of Chili pepper (Capsicum chinense Jacquin.) is affected by biotic and abiotic stresses. Abiotic stress such as waterlogging increases the expression of ACS (1-aminocyclopropane-1-carboxylic acid synthase) enzyme. This enzyme plays a great role in the process of ethylene biosynthesis and encoded by multiple genes. Waterlogging causes hypoxia condition. One of ACS enzymes that responds to hypoxia condition is ACS2. The different respond to hypoxia stress among plant was assumed to be caused by a different structure of the ACS2 enzyme. This study aimed to identify and confirm the active site of Capsicum chinense Jacquin ACS2  using modeling and in silico docking. The result of the three-dimensional (3D) structure modeling showed 91% similarity of Capsicum chinense Jacquin ACS2 with the structure of the tomato (Solanum lycopersicum L.). The Capsicum chinense ACS2 confirmed five active sites that bind to the substrates asparagin396B, valine397B, thyrosine152B, threonine128B, and thyrosine92A. 
Socialization of the Use of Social Media Properly and Correctly Without Violating the ITE Law for Malang City Residents Ali, Moh.; Priyanti, Novie Ary; Habiburrahman , Novan; Puspaningsih, Eunike Sri; Lestari, Juniarti Wulan; Hariadi , Agung; Ferdinant Pakage
⁠International Journal of Asia Pacific Community Service Vol. 1 No. 1 (2024)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/46ns9p48

Abstract

Constitution number 19 of 2016 concerning Information and Electronic Transactions (hereinafter abbreviated as "ITE") in this dynamic era. This is considered important because of this sophisticated era, especially among young people (especially students) who are inseparable from technology, especially social media, which can have positive and negative impacts, so it is necessary to inform them that there are legal provisions (both civil and criminal) that can threaten them if they are not careful in using social media to convey expressions or opinions. The problems discussed are regarding students' and teachers' understanding of ITE regulations and the application of sanctions if they violate or commit ITE crimes. So that with this socialization, it is hoped that it can be a learning experience for Malang city residents to be wiser in using social media. This activity was carried out offline and was carried out for two days. The conclusion that can be drawn is that the awareness of Malang city residents regarding their knowledge of ITE regulations is still low, so this activity is considered as a new provision so that they can be more careful in accessing social media.
Discovery-Inquiry Learning Model in Delivering Living Creature Classification Material for High School/Equivalent Schools Priyanti, Novie Ary; Pratama, Raditya; Puspaningsih, Eunike Sri; Rahmania, Ika; Niswatus Sufiyah; Liza Choirun Nisa'; M. Zainul Muttaqin; Sarlotha Selan
International Journal of Sustainable English Language, Education, and Science Vol. 1 No. 1 (2024)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/zd991w62

Abstract

This research provides an understanding of why the Discovery-Inquiry learning method as a means to deliver the classification of living things. This research uses a descriptive qualitative approach, descriptive by conveying clearly what is meant by discovery-inquiry and why it is appropriate when implemented in the material of the diversity of living things. Education is a planned and conscious effort to create a learning atmosphere and learning process in which students actively develop their potential, discovery-inquiry is an option in its delivery. Discovery-inquiry is a learning process where a concept is not presented in its final form, but students must organize their own learning by finding out these concepts. Meanwhile, the classification of living things classifies living things based on certain characteristics. So, of course it is very related to the discovery-inquiry method which requires students to play a greater role than the teacher by finding and ensuring the object under study whether it is in accordance with the theory or finding something new.
Fortification of Yogurt with Moringa Leaf Extract (Moringa oleifera) on Quality and Quality as a Functional Food: A Review Azis, Rosidi; Lestari, Juniarti Wulan; Puspaningsih, Eunike Sri; Muqtafiah
⁠International Journal of Sustainable Social Culture, Science Technology, Management, and Law Humanities Vol. 2 No. 1 (2025)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/3a431y62

Abstract

The incorporation of natural bioactive compounds into dairy products has gained increasing attention due to their potential to enhance nutritional value and functional properties. Moringa oleifera, commonly known as the drumstick tree, is a nutrient-dense plant rich in polyphenols, flavonoids, vitamins, and minerals, making it a promising candidate for yoghurt fortification. This review evaluates the impact of Moringa oleifera leaf extract on the physicochemical, microbiological, antioxidant, and sensory attributes of yoghurt. Data from recent studies (2014 - 2024) indicate that the addition of Moringa oleifera extract at concentrations up to 0.5% significantly improves total phenolic content, antioxidant activity (DPPH and ABTS assays), water holding capacity (WHC), and viscosity, while maintaining acceptable pH levels and supporting probiotic viability. However, higher concentrations may lead to undesirable sensory characteristics such as astringency and greenish coloration, which can affect consumer acceptance. Optimization of extraction methods, fortification levels, and formulation strategies such as encapsulation or blending with other natural ingredients is recommended to balance functionality and sensory appeal. Overall, yoghurt fortified with Moringa oleifera extract demonstrates potential as a functional food with enhanced antioxidant properties and improved nutritional profile. Further research is needed to assess long-term stability and in vivo health benefits.