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All Journal Jurnal Laboratorium Khatulistiwa Jurnal teknologi Kesehatan Borneo
Sugito Sugito
Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Combination of Aloe Vera and Chocolate to Reduce The Number of The Colony of Staphylococcus Aureus Edy Suwandi; Ari Nuswantoro; Sugito Sugito
Jurnal teknologi Kesehatan Borneo Vol 3 No 2 (2022): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v3i2.197

Abstract

Aloe vera (AV) and chocolate are good examples of functional foods because they contain prebiotics that can help the host defend itself against harmful bacterial infections such as Staphylococcus aureus. Many studies have used AV or chocolate alone to help fight infection, but not much is known about using both together. This study aims to count the number of colonies of S. aureus infected on mice’s skin (Mus musculus) after being given AV and cocoa in certain doses per kilogram of body weight. A total of 35 healthy male mice were infected with S. aureus in the superficial area of ​​the nape of the skin. After that, they were divided into seven groups, each consisting of 5 mice, and given the following treatments: given 50 mg/kg BW AV (group A), 50 mg/kg BW brown (B), 50 mg/kg BW combination of AV and chocolate (C), given 75 mg/kg BW AV (D), given 75 mg/kg BW brown (E), given 75 mg/kg BW combination of AV and chocolate (F), and control (without prebiotic administration), K). Mice that were given 75 mg/kg BW AV/chocolate/AV+chocolate had a better ability to reduce the number of bacteria than those that were given 50 mg/kg BW AV/chocolate/AV+chocolate. In addition, rats that were given AV and chocolate together had a better ability to reduce the number of bacteria than those that were given either only AV or chocolate alone. Prebiotics are both found in AV and chocolate but with different substances. This causes mice given a combination of prebiotics to be considered better than those given only one type of prebiotic source.
Antibakteri Deodorant Spray Minyak Atsiri Bunga Cengkeh terhadap Staphylococcus aureus Dhifa Wira Wicaksana; Edy Suwandi; Etiek Nurhayati; Sugito Sugito; Imma Fatayati; Emilda Sari
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2127

Abstract

Body odor is a common problem that can affect personal comfort and social confidence. Understanding the relationship between clove flower essential oil concentration and the inhibition of Staphylococcus aureus growth is important for improving the effectiveness of natural deodorant formulations. This study aimed to analyze the relationship between the concentration of clove flower essential oil deodorant spray and the inhibition of Staphylococcus aureus among samples tested at the laboratory. This study used an experimental design with a total sample of 30 samples. Data were collected using the bacterial culture diffusion method on Mueller Hinton Agar (MHA) media and analyzed using the simple linear regression test with a significance level of p < 0.05. The results showed that 15% of samples demonstrated antibacterial activity. Statistical analysis revealed a significant relationship between clove flower essential oil deodorant spray concentration and inhibition of Staphylococcus aureus growth (p = 0.000). The concentration of clove flower essential oil deodorant spray significantly influences the inhibition of Staphylococcus aureus. Early development and utilization of natural-based deodorant formulations are recommended to improve product effectiveness and safety.
Co-Authors Ari Nuswantoro Dhifa Wira Wicaksana Emilda Sari Etiek Nurhayati Imma Fatayati Suwandi, Edy