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All Journal Jurnal Agrotek Tropika AL-HAYAT: Journal of Biology and Applied Biology Jurnal Pengembangan Agroindustri Terapan
sigit ardiansyah
Politeknik Negeri Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Industrial & Manufacturing Engineering Other

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Toxicity Of Jatropha curcas and Cymbopogon nardus Extracts Against Pests Callosobruchus chinensis On Mung Beans sigit ardiansyah; Ni Siluh Putu Nuryanti; Anung Wahyudi
Al-Hayat: Journal of Biology and Applied Biology Vol 5, No 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.12503

Abstract

Callosobruchus chinensis L. is an important pest on mung beans. Alternative control of C. chinensis using botanical insecticides from extracts of Jatropha curcas and Cymbopogon nardus. The purpose of the research was to compare the toxicity of extracts and determine their compatibility. Toxicity testing methods use to contact, fumigation, and seed dressing methods. The research data was tested by probit analysis. The results showed that the toxicity of the contact method extract was more toxic than fumigation and seed dressing. The highest LC50 and LC95 values for the C. nardus extract applied with the contact method were observed at 72 HAT at 0.17% and 0.40% more toxic than the J. curcas extract at 0.21% and 2.14%. The best mixed extract of J. curcas and C. nardus was at a ratio of 2:1 with strong synergistic interaction (LC50) and weak synergy (LC95).
AN UTILIZATION OF AGRICULTURAL WASTE AS A SUBSTRATE FOR PRODUCING CELLULASE ENZYME BY ASPERGILLUS NIGER Nurma Pratiwi; Sigit Ardiansyah
Jurnal Pengembangan Agroindustri Terapan Vol 1 No 1 (2022)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jupiter.v1i1.2655

Abstract

Fresh Fruit Bunches (FFB) of oil palm, bran, straw, and bagasse are agricultural wastes whose availability is very abundant in Indonesia. The agricultural waste is lignocellulosic waste which still has economic value if further processing is carried out, namely as a substrate in the production of cellulase enzymes. Cellulase enzymes are commonly used in various industries such as food biotechnology, textiles, animal feed, paper, and agriculture to degrade cellulose with its main products, namely glucose, cellobiose, and cellooligosaccharides. In producing cellulase enzymes, it is necessary to have microorganisms that have a high ability to produce enzymes, one of which is Aspergillus niger. The purpose of this study was to determine the activity of crude cellulase enzyme, protein content, and specific activity of cellulase enzyme from agricultural waste which includes FFB, bran, straw, and bagasse. The research methods included preparation of Aspergillus niger culture, delignification, basal medium preparation, cellulase enzyme production, enzyme extraction, crude cellulase enzyme activity test (CMC-ase), lowry method protein content test, and determination of cellulase enzyme specific activity. The study showed that the highest crude cellulase enzyme activity in bran was 26.83 U/ml, the highest protein content in bagasse was 63.42 g/ml, and the highest specific activity of cellulase enzyme in straw was 0.9818 U/ml. The high enzyme activity is influenced by the cellulose content in the material, type of substrate, media, substrate concentration, pH, and temperature.
TOKSISITAS CAMPURAN MINYAK ATSIRI BIJI JARAK PAGAR DAN SERAI WANGI TERHADAP Callosobruchus chinensis Sigit Ardiansyah; Ni Siluh Putu Nuryanti; Anung Wahyudi
Jurnal Agrotek Tropika Vol 12, No 3 (2024): JURNAL AGROTEK TROPIKA VOL 12, AGUSTUS 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i3.6840

Abstract

Callosobruchus chinensis L. merupakan hama gudang yang dapat menurunkan kualitas dan kuantitas produksi kacang hijau. Biasanya pengendalian C. chinensis dengan insektisida kimia, namun cara tersebut dapat menyebabkan masalah lingkungan dan risiko keamanan pangan. Sehingga, perlu pengadaan insektisida alternatif yang lebih aman dan ramah lingkungan yaitu menggunakan insektisida nabati. Biji jarak pagar dan serai wangi memiliki potensi yang besar sebagai insektisida nabati. Penelitian bertujuan untuk menguji toksisitas, penghambatan peneluran dan sifat interaksi ekstrak campuran biji jarak pagar dan serai wangi dalam berbagai rasio sebagai insektisida nabati terhadap hama C.  chinensis pada biji kacang hijau. Metode penelitian meliputi perbanyakkan C. chinensis, pembuatan ekstrak, formulasi insektisida nabati, uji toksisitas ekstrak tunggal dan campuran, uji penghambatan peneluran, dan uji kompatibilitas ekstrak campuran. Hasil Penelitian menunjukkan bahwa toksisitas ekstrak campuran biji jarak pagar dan serai wangi pada rasio 2 : 1 lebih toksik daripada rasio 1 : 2 dan 1 : 1. Ekstrak campuran biji jarak pagar dan serai wangi pada rasio 2 :1 dengan konsentarsi 1%  mampu menghambat peneluran sebesar 100%, Sifat Interaksi ekstrak campuran biji jarak pagar dan serai wangi terhadap C. chinensis terbaik pada rasio 2:1, sifat interaksi pada LC50 sinergi kuat dan pada LC95 sinergi lemah.
Co-Authors Anung Wahyudi Ni Siluh Putu Nuryanti Ni Siluh Putu Nuryanti Nurma Pratiwi