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AN UTILIZATION OF AGRICULTURAL WASTE AS A SUBSTRATE FOR PRODUCING CELLULASE ENZYME BY ASPERGILLUS NIGER Nurma Pratiwi; Sigit Ardiansyah
Jurnal Pengembangan Agroindustri Terapan Vol 1 No 1 (2022)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jupiter.v1i1.2655

Abstract

Fresh Fruit Bunches (FFB) of oil palm, bran, straw, and bagasse are agricultural wastes whose availability is very abundant in Indonesia. The agricultural waste is lignocellulosic waste which still has economic value if further processing is carried out, namely as a substrate in the production of cellulase enzymes. Cellulase enzymes are commonly used in various industries such as food biotechnology, textiles, animal feed, paper, and agriculture to degrade cellulose with its main products, namely glucose, cellobiose, and cellooligosaccharides. In producing cellulase enzymes, it is necessary to have microorganisms that have a high ability to produce enzymes, one of which is Aspergillus niger. The purpose of this study was to determine the activity of crude cellulase enzyme, protein content, and specific activity of cellulase enzyme from agricultural waste which includes FFB, bran, straw, and bagasse. The research methods included preparation of Aspergillus niger culture, delignification, basal medium preparation, cellulase enzyme production, enzyme extraction, crude cellulase enzyme activity test (CMC-ase), lowry method protein content test, and determination of cellulase enzyme specific activity. The study showed that the highest crude cellulase enzyme activity in bran was 26.83 U/ml, the highest protein content in bagasse was 63.42 g/ml, and the highest specific activity of cellulase enzyme in straw was 0.9818 U/ml. The high enzyme activity is influenced by the cellulose content in the material, type of substrate, media, substrate concentration, pH, and temperature.
Characterization of Biobriquette from Carbonized Durian Peel Using Coconut Shell as the Binder Giffary Pramafisi Soeherman; Pridata Gina Putri; Deary Amethy Zahrotinufus Joen; Iyan Indrawan; Nurma Pratiwi
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 3 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v7i3.347

Abstract

This research aimed to study the characteristics of carbonized durian peel biobriquette using tar as a binder. A 1:10 ratio of binder and carbonized durian peel was used. The 25, 50, and 75% (coded as P1, P2, and P3, respectively) tar were used as a binder alongside tapioca flour to produce a binder with different tar concentrations. The briquette characteristics determined moisture content, ash content, calorific value, density, and water-absorbing capacity. Moisture content ranged from 9.32% to 9,41% for treatments P1 to P3, while the ash content ranged from 12,29% to 13,09%, showing no significant difference among the treatments. Massive difference was observed in calorific value, as P1 gives 5106/35 cal g-1 calorific value while P2 and P3 give 9267.56 and 9694.53 cal g-1, respectively. The density observed was relatively low, ranging from 0.5029 g cm-3 to 0,5685 g cm-2. As for water-absorbing capacity, P3 absorbed the least amount of water, 29.43%. From this research, we can conclude that coconut shell tar has the potential to be utilized as a binder in forming biobriquette from carbonized durian peel.
Pengolahan Limbah Cair Berbasis Zero Waste Pada Industri Minyak Pala Menjadi Cairan Desinfektan Azalia, Ailsa Ailsa; Subandi, Subandi; Mulia H.H, M. Perdiansyah; Pratiwi, Nurma
JTPG (Jurnal Teknologi Pertanian Gorontalo) Vol 10 No 1 (2025): Jurnal JTPG (Mei)
Publisher : PROGRAM STUDI MESIN DAN PERALATAN PERTANIAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30869/jtpg.v10i1.1438

Abstract

Indonesia memiliki potensi besar dalam pengolahan minyak atsiri pala, khususnya di provinsi Lampung. Proses penyulingan minyak atsiri pala menghasilkan limbah cair yang disebut hidrosol. Sampai saat ini, hidrosol belum dimanfaatkan secara optimal. Penelitian ini bertujuan untuk mengembangkan konsep pengelolaan limbah berbasis zero waste melalui proses diversifikasi produk untuk menjadi cairan desinfektan yang berbahan baku utama yaitu hidrosol. Hidrosol pala mengandung senyawa aktif seperti DEHA, asam benzoat, dan terpenoid yang berpotensi sebagai agen antimikroba. Metode yang digunakan dalam penelitian ini meliputi analisis neraca massa untuk memetakan keseimbangan material selama proses produksi dan formulasi cairan desinfektan melalui metode eksperimental deskriptif. Analisis produk dilakukan secara kualitatif deskriptif . Hidrosol dari limbah penyulingan minyak atsiri pala digunakan sebagai bahan utama dan selanjutnya ditambahkan bahan kimia seperti NaOH, arpus, camperlan, dan aquades untuk menghasilkan cairan desinfektan. Analisis produk mencakup stabilitas larutan, warna, dan aroma. Hasil penelitian menunjukkan bahwa limbah cair yang selama ini dibuang dapat dimanfaatkan menjadi produk yang bernilai tambah. Cairan desinfektan yang dihasilkan memiliki stabilitas larutan yang baik, berwarna kuning transparan, dan memiliki aroma khas pala hal ini sesuai dengan karakteristik cairan desinfektan secara fisik. Penerapan prinsip zero waste tidak hanya meminimalkan limbah tetapi juga meningkatkan efisiensi dan peluang pendapatan industri. Penelitian ini memberikan solusi ramah lingkungan untuk pengelolaan limbah agroindustri minyak atsiri pala sekaligus mendukung diversifikasi produk untuk kebutuhan sanitasi.
Characterization of Biobriquette from Carbonized Durian Peel Using Coconut Shell as the Binder Soeherman, Giffary Pramafisi; Putri, Pridata Gina; Joen, Deary Amethy Zahrotinufus; Indrawan, Iyan; Pratiwi, Nurma
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 3 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v7i3.347

Abstract

This research aimed to study the characteristics of carbonized durian peel biobriquette using tar as a binder. A 1:10 ratio of binder and carbonized durian peel was used. The 25, 50, and 75% (coded as P1, P2, and P3, respectively) tar were used as a binder alongside tapioca flour to produce a binder with different tar concentrations. The briquette characteristics determined moisture content, ash content, calorific value, density, and water-absorbing capacity. Moisture content ranged from 9.32% to 9,41% for treatments P1 to P3, while the ash content ranged from 12,29% to 13,09%, showing no significant difference among the treatments. Massive difference was observed in calorific value, as P1 gives 5106/35 cal g-1 calorific value while P2 and P3 give 9267.56 and 9694.53 cal g-1, respectively. The density observed was relatively low, ranging from 0.5029 g cm-3 to 0,5685 g cm-2. As for water-absorbing capacity, P3 absorbed the least amount of water, 29.43%. From this research, we can conclude that coconut shell tar has the potential to be utilized as a binder in forming biobriquette from carbonized durian peel.
Utilization of Wastewater from Air Conditioner Circulation Systems for Clean Water Energy Efficiency and Industrial Equipment Maintenance Subandi; Azalia, Ailsa; Shaffira, Mutia Rizkia; Pratiwi, Nurma
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 9 No. 1 (2025)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v9i1.563

Abstract

There is significant wasteful use of clean water for operating equipment during field and laboratory practice. Additionally, damage to laboratory equipment has been reported due to the use of untreated water. These issues, along with previous research on managing air conditioner (AC) wastewater at the Lampung State Polytechnic, form the basis for our initiative to utilize AC wastewater as a sustainable resource. Proper management of this abundant water source can support the operation and maintenance of laboratory equipment. The research findings demonstrate that circulating AC wastewater to operate a Soxhlet extraction system for 4.5 hours can reduce clean water consumption by 0.65 cubic meters, equivalent to a cost savings of IDR 43,200. Furthermore, the results indicate improved efficiency, effectiveness, and overall equipment performance when using AC wastewater compared to conventional methods. Contribution to Sustainable Development Goals (SDGs) SDG 6 – Clean Water and SanitationSDG 9 – Industry, Innovation, and Infrastructure:SDG 12 - Responsible Consumption and ProductionSDG 13 - Climate Action
Application of Bioplastic with Corn Cob Cellulose and Chitosan as Active Packaging for Pempek Products Pratiwi, Nurma; Putri, Pridata Gina; Fahrulsyah; Agassi, Taufik Nugraha; Joen, Deary Amethy Zahrotinufus
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 9 No. 3 (2025)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v9i3.845

Abstract

Pempek is a fish-based food product that is highly susceptible to spoilage due to its elevated water and protein content, which fosters microbial growth. The reliance on conventional plastic packaging, such as polypropylene, raises environmental concerns, underscoring the need for alternative, more eco-friendly packaging solutions that enhance product safety. This study aimed to utilize bioplastics derived from corn cob cellulose and chitosan as active packaging to mitigate the deterioration of pempek during storage. The research involved extracting corn cob cellulose via the organosolv method, fabricating bioplastics via solution casting, packaging the pempek, and assessing Total Plate Count (TPC) and pH levels over 4 days of storage at room temperature. The findings revealed that the active bioplastic significantly inhibited microbial growth. The TPC of pempek packaged in bioplastic was 1–2 logs lower than that of the control on days 2-4. Additionally, the pH of the bioplastic-preserved pempek declined from 6.75 to 5.46, whereas the control increased from 7.77 to 8.17, indicating microbial spoilage. These results suggest that bioplastic incorporating corn cob cellulose and chitosan effectively slows deterioration, enhances microbiological safety, and presents a viable, environmentally friendly packaging alternative for food products. Contribution to Sustainable Development Goals (SDGs):SDG 12 – Responsible Consumption and ProductionSDG 13 – Climate ActionSDG 14 – Life Below WaterSDG 15 – Life on Land