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Effect of breeds on semen characteristics of aged bulls in the Indonesian National Artificial Insemination Center Novianti, Irida; Purwantara, Bambang; Herwijanti, Enniek; Nugraha, Chairdin Dwi; Putri, Rafika Febriani; Furqon, Ahmad; Septian, Wike Andre; Rahayu, Sri; Nurgiartiningsih, Veronica Margareta Ani; Suyadi, Suyadi
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 2 (2020): August 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.02.10

Abstract

Madura and Bali cattle are two of Indonesian indigenous breeds. One possible method to increase their productivity is through artificial insemination (AI) using frozen semen. This study conducted at the Singosari National Artificial Insemination Center, East Java, Indonesia, to evaluate the semen characteristics of different bull breeds ranging in age from 7 to 10 years and to provide useful recommendations regarding the efficacy of older cattle. A total of 2016 ejaculation data recorded from Indonesian indigenous cattle (Madura and Bali bulls) and Bos taurus bulls (Simmental) were used in this study to analyze semen volume, sperm motility, and sperm concentration. Results revealed differences in semen volume, sperm motility, and sperm concentration among bull breeds (p < 0.05). Simmental bulls have the highest semen volume (p < 0.05) compared with Bali and Madura bulls. Base on sperm motility, Bali bulls showed the highest average value (p < 0.05) compared with Simmental and Madura bulls. Sperm concentration was the highest (p < 0.05) among Bali bulls compared with Simmental and Madura bulls. Another important finding was that the average semen volume, sperm motility, and sperm concentration of older bulls fulfilled the standard criteria established for frozen semen processing. Therefore, it would be possible to improve the performance of these breeds through artificial insemination using frozen semen of Madura and Bali bulls, which can be more widely distributed to farmers to increase their herd productivity. This study also indicates that Indonesian indigenous bulls have superior semen characteristics than Simmental bulls.
Pengaruh Thawing terhadap Kualitas Semen Kambing Boer Menggunakan Pengencer Tris-aminomethane yang Disuplementasi Antioksidan Quercetin Sari, Rossa Purnama; Nugraha, Chairdin Dwi; Suyadi, Suyadi
Jurnal Sain Peternakan Indonesia Vol 19 No 1 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.19.1.11-20

Abstract

This study aims to determine the effect of the thawing technique on the quality of Boer goat semen with tris aminomethane diluent supplemented with quercetin antioxidants. The materials used in this research are the semen of a Boer goat, which was collected with an artificial vagina. This study used experimental laboratory methods. The design pattern used is a Factorial Complete Randomized Design, which consists of two factors. Each treatment was repeated 4 times. The first factor is the thawing temperature with 3 levels of treatment those were 32°C, 37°C, and 40°C. The second factor is the thawing time with 3 treatment levels: 30 seconds, 60 seconds and 90 seconds. The variables were the motility percentage, viability, abnormality and membrane integrity. The results showed that thawing temperature had a significantly different effect (P<0.01) on the percentage of motility, viability, abnormality and membrane integrity. Thawing time had a significantly different effect (P<0.01) on the percentage of motility, abnormality and membrane integrity and gave affects significant (P<0.05) in the percentage of viability. Meanwhile, the interaction between thawing temperature and time is insignificant (P>0.05). The best result shown after thawing is in treatment thawing temperature at 37°C and treatment thawing time at 30 seconds.
Quercetin Supplementation in Andromed Extender on Quality of Boer Goat Semen during Room Temperature Storage Madhyastha, Wandha; Nugraha, Chairdin Dwi; Suyadi, Suyadi
Jurnal Ilmu Peternakan Terapan Vol 8 No 1 (2024): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v8i1.4776

Abstract

This research aimed to determine the effect of quercetin antioxidant concentration levels on Boer goat semen quality during room temperature storage using andromed extender. Fresh semen was collected from two of 5-years-old Boer goats with weight of 60 and 70 kg. The method of this research was an experimental laboratory using Completely Randomized Design (CRD) Factorial of two factors, namely the treatment of quercetin concentration levels at 0 µM, 10 µM, 20 µM and 30 µM and the storage time during room temperature at 0 hour, 2 hours, 4 hours, 6 hours and 8 hours. The variables were observed based on individual motility, viability, abnormalities and membrane integrity of spermatozoa. The result showed that the antioxidant quercetin 30 µM have a significant effect to individual motility (P<0.05) but did not have a significant effect to the viability and abnormality of spermatozoa. Antioxidant quercetin gave a highly significant difference on membrane integrity (P<0.01). Storing semen at room temperature for 6 hours resulted in a highly significant difference in individual motility, abnormality and membrane integrity (P<0.01) but did not show a significant difference to viability. The best result showed that the antioxidant quercetin 30 µM was able to maintain the quality of spermatozoa at room temperature storage up to 6 hours.
Pengaruh lama ekuilibrasi suhu dingin dan uap nitrogen cair terhadap kualitas semen kambing boer menggunakan tris-aminomethane yang disuplementasi antioksidan quercetin: Effect of duration in cold temperature and liquid nitrogen vapor equilibration on boer goats semen quality following dillution using tris-aminomethane supplemented antioxidant quercetin Norfaiziyah, Rahmi; Nugraha, Chairdin Dwi; Suyadi, Suyadi
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 14 No. 2 (2024): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v14i2.394

Abstract

Abstract  Inappropriate equilibration time will reduce the quality of spermatozoa. The aim of this research were knowing the effect of different cold temperature equilbration and liquid nitrogen vapor equilibration time to the quality of Boer goat semen after thawing using tris aminomethane that suplemented with antioxidant quercetin. Semen from 5-year-old, 70 kg Boer goats was equilibrated for 1 hours, 1.5 hours, 2 hours, 2.5 hours at 4˚C and for 5 minutes, 10 minutes, 15 minutes in liquid nitrogen vapor. This study used laboratory experimental methods using Completely Randomized Design 4 x 3 factorial. Observed parameters are motility, viability, abnormality, and membran integrity after thawing. The result showed that 2.5 hours cold temperature equilibration has the best percentage of individual motility, viability, abnormality, and membran integrity after thawing respectively for 40.00±12.06%, 69.58 ± 3.16%, 2.58±0.41%, 31.50±3.10% while 5 minutes in liquid nitrogen vapor has the best percentage individual motility, viability, abnormality, and membran integrity after thawing respectively for 36.88±12.23%, 67.39±5.16%, 2.61±0.34%, 29.53±4.46%. The conclusion is cold temperature equilibration for 2.5 hours and nitrogen vapor equilibration time for 5 minutes are the optimal time treatment combinations for spermatozoa to adjust with low temperatures and maintaining quality after thawing. Keywords: Boer goat; Cold temperature equilibration; Liquid nitrogen vapor equilibratio; Quercetin; Sperm quality   Abstrak  Waktu ekuilibrasi yang tidak tepat akan menurunkan kualitas spermatozoa. Penelitian bertujuan untuk mengetahui pengaruh perbedaan ekuilibrasi suhu dingin dan ekuilibrasi pada uap nitrogen cair terhadap kualitas semen kambing Boer setelah dicairkan menggunakan tris aminomethane yang disuplementasi dengan antioksidan quercetin. Materi penelitian menggunakan semen segar yang diambil dari kambing Boer berumur 5 tahun dengan berat badan 70 kg. Penelitian ini menggunakan metode percobaan laboratorium dengan jenis Rancangan Acak Lengkap Faktorial 4 x 3. Faktor pertama adalah waktu kesetimbangan suhu dingin 4˚C (1 jam, 1,5 jam, 2 jam, 2,5 jam). Faktor kedua adalah kesetimbangan uap nitrogen cair (5 menit, 10 menit, 15 menit). Parameter yang diamati adalah motilitas individu, viabilitas, abnormalitas, dan integritas membran spermatozoa setelah thawing. Hasil penelitian menunjukkan bahwa waktu  2,5 jam memiliki persentase motilitas, viabilitas, abnormalitas, dan integritas membran individu terbaik setelah thawing masing-masing sebesar 40,00±12,06%, 69,58±3,16%, 2,58±0,41%, 31,50±3,10%. Waktu 5 menit dalam memiliki persentase motilitas individu, viabilitas, abnormalitas, dan integritas membran terbaik setelah thawing masing-masing sebesar 36,88±12,23%, 67,39±5,16%, 2,61±0,34%, 29,53±4,46%. Kesimpulannya adalah waktu ekuilibrasi suhu dingin selama 2,5 jam dan waktu ekuilibrasi uap nitrogen selama 5 menit merupakan kombinasi perlakuan waktu yang optimal bagi spermatozoa untuk menyesuaikan diri dengan suhu rendah dan menjaga kualitas setelah thawing. Kata kunci: Ekuilibrasi suhu dingin; Ekuilibrasi uap nitrogen cair; Kambing boer; Kualitas sperma; Quercetin