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Effect of breeds on semen characteristics of aged bulls in the Indonesian National Artificial Insemination Center Novianti, Irida; Purwantara, Bambang; Herwijanti, Enniek; Nugraha, Chairdin Dwi; Putri, Rafika Febriani; Furqon, Ahmad; Septian, Wike Andre; Rahayu, Sri; Nurgiartiningsih, Veronica Margareta Ani; Suyadi, Suyadi
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 2 (2020): August 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.02.10

Abstract

Madura and Bali cattle are two of Indonesian indigenous breeds. One possible method to increase their productivity is through artificial insemination (AI) using frozen semen. This study conducted at the Singosari National Artificial Insemination Center, East Java, Indonesia, to evaluate the semen characteristics of different bull breeds ranging in age from 7 to 10 years and to provide useful recommendations regarding the efficacy of older cattle. A total of 2016 ejaculation data recorded from Indonesian indigenous cattle (Madura and Bali bulls) and Bos taurus bulls (Simmental) were used in this study to analyze semen volume, sperm motility, and sperm concentration. Results revealed differences in semen volume, sperm motility, and sperm concentration among bull breeds (p < 0.05). Simmental bulls have the highest semen volume (p < 0.05) compared with Bali and Madura bulls. Base on sperm motility, Bali bulls showed the highest average value (p < 0.05) compared with Simmental and Madura bulls. Sperm concentration was the highest (p < 0.05) among Bali bulls compared with Simmental and Madura bulls. Another important finding was that the average semen volume, sperm motility, and sperm concentration of older bulls fulfilled the standard criteria established for frozen semen processing. Therefore, it would be possible to improve the performance of these breeds through artificial insemination using frozen semen of Madura and Bali bulls, which can be more widely distributed to farmers to increase their herd productivity. This study also indicates that Indonesian indigenous bulls have superior semen characteristics than Simmental bulls.
Genetic Diversity of Bali Cattle Based on Microsatellite Marker in Indonesian Breeding Centre Wike andre Septian; . Jakaria; Cece Sumantri
Media Peternakan Vol. 38 No. 1 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1463.008 KB) | DOI: 10.5398/medpet.2015.38.1.12

Abstract

Genetics characterization in livestock based on microsatellite has been widely implemented including for Bali cattle in three different breeding centres in Indonesia. This study aimed to determine diversity of Bali cattle microsatellites in three breeding centers namely BPTU Bali cattle in Bali, BPT-HMT Serading Sumbawa in West Nusa Tenggara, and Village Breeding Center in South Sulawesi. The number of animals used in this study was 95 head of cattle consisted of 32 heads from BPTU Bali Province, 32 heads from BPT-HMT Serading Sumbawa, and 31 heads from Village Barru Breeding Center Barru distric. Microsatellite loci used to determine the diversity was the locus SPS115, INRA037, MM12, and ETH185 based on flourescently labeled fragment method. Data analysis of microsatellite in Bali cattle at three different locations was performed by using POPGEN 1.2, Cervus, and POPTREE2 programs. The results showed that microsatellite diversity in Bali cattle detected 32 alleles from three different locations, and there were specific alleles at each location. Average values of observed heterozygosity (Ho) and expected heterozygosity (He) were 0.418 and 0.604 respectively, while the average value of polymorphism informative content (PIC) was 0.579. The Hardy-Weinberg equilibrium in general suggested that the loci used in the Bali cattle in three populations were in equilibrium, except INRA037 and ETH185. The genetic diversity between populations of Bali cattle was 0.033 (3%), while the inbreeding coefficient index in all populations was 0.296 (29.6 %). Bali cattle phylogeny tree with three populations showed that the populations of Bali cattle in BPTU Bali and VBC Barru had close genetic distance compared to the population of Bali cattle in BPT-HMT Serading Sumbawa. The results of this study provide information that the characteristics of Bali cattle breeding centers in three locations are different, so we need a directed breeding program in each population.Key words: Bali cattle, genetics diversity, microsatellite
Single Nucleotide Polymorphims (snps) Identification of Inhibin Sub Unit-α (inha) Gene on Madura Bulls Irida Novianti; Chairdin Dwi Nugraha; Rafika Febriani Putri; Ahmad Furqon; Wike Andre Septian; S. Rahayu; V. M. A. Nurgiartiningsih; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.10

Abstract

INHA gene is a gene that is suggested to have role in reproductive system. Single Nucleotide Polymorphisms (SNPs) of Madura Bulls were identified in this study. Polymerase Chain Reactions (PCR) was used to amplify INHA gene region and MEGA 7 program was utilized to align the amplified region sequences with sequence from Ensembl database. Four SNPs found in INHA and they are located at the first exon. Two SNPs were misssense mutations that causing the substitution of amino acid leucine21 by proline, and amino acid valine63 by methionine and the other two SNPs were synonymous mutation. One of the synonymous SNPs was a novel mutation. Based on those identified SNPs, they could be suggested as potential candidate markers of reproduction traits for Madura bulls. Moreover, through heterozygosity value from the observed bulls, it was indicated that the genotype was varied in population. Therefore a molecular selection program could be designed to determine the Madura superior bull.
Identifikasi Keragaman Genetik Gen Inhibin Subunit Alpha (INHA) dan Inhibin Subunit Beta A (INHBA) Pada Sapi Peranakan Ongole (PO) Rafika Febriani Putri; Chairdin Dwi Nugraha; Ahmad Furqon; Wike Andre Septian; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.9

Abstract

Gen Inhibin berperan dalam mengatur proliferasi sel, pertumbuhan kelenjar adrenal, hematopoiesis dan metabolisme tulang. Inhibin subunit alpha juga diketahui memiliki dampak yang signifikan terhadap berat badan, panjang tubuh dan lingkar badan. Penelitian ini bertujuan untuk mengidentifikasi keragaman gen INHA dan INHBA pada Sapi PO yang berturut-turut sebanyak 68 ekor sapi PO yang berasal dari UPT PT HMT Tuban dan 11 ekor sapi PO BBIB Singosari. Metode pendeteksian keragaman gen menggunakan metode sequencing. Hasil penelitian menunjukkan bahwa pada sapi PO populasi UPT Tuban ditemukan tiga genotipe (AA, AB dan BB) pada fragmen gen INHA dan INHBA, sapi PO populasi BBIB Singosari memiliki satu genotipe (AA) pada fragmen gen INHA serta dua genotipe (AA dan BB) pada fragmen gen INHBA. Berdasarkan hasil tersebut dapat disimpulkan bahwa gen INHA dan INHBA pada sapi PO populasi UPT Tuban dalam kondisi beragam (polimorfik). Informasi dalam penelitian ini dapat dijadikan dasar atau landasan dalam memulai program seleksi dan perkawinan sapi potong lokal Indonesia berbasis teknologi molekuler.
Analisis Hubungan Bobot Badan Terhadap Produksi Semen Segar Sapi Bali di Balai Besar Inseminasi Buatan-Singosari Chairdin Dwi Nugraha; Enniek Herwijanti; Irida Novianti; Ahmad Furqon; Wike Andre Septian; Woro Busono; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 20, No 1 (2019): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2019.020.01.9

Abstract

Sapi asli indonesia dengan populasi terbesar dibandingkan sapi lokal lainnya yaitu sapi Bali. Pengembangan sapi Bali secara berkelanjutan dengan cara meningkatkan kualitas genetik melalui aspek reproduksi dalam pemilihan pejantan unggul. Bobot badan adalah salah satu kriteria dalam memilih pejantan unggul. Penelitian ini bertujuan untuk menganalisis hubungan bobot badan dengan kualitas semen. Materi yang digunakan yaitu data sekunder mulai tahun 2012-2018 dari 50 ekor pejantan sapi Bali meliputi data kualitas semen sebanyak (n=1204) ejakulasi di Balai Besar Inseminasi Buatan, Singosari, Jawa Timur, Indonesia. Metode penelitian adalah studi kasus. Variabel yang diamati yaitu bobot badan, volume semen, motilitas individu dan konsentrasi semen. Data dianalisa dengan menggunakan SPSS 24 dengan metode One-Way ANOVA. Uji korelasi menggunakan korelasi Pearson (r) dan analisis statistik model regresi linier untuk menduga kualitas semen berdasarkan bobot badan. Hasil penelitian menunjukkan bahwa bobot badan sapi Bali mempengaruhi secara signifikan (p<0,05) terhadap kualitas semen. Semakin tinggi bobot badan maka nilai volume dan motilitas meningkat kecuali konsentrasi semen yang sangat fluktuatif. Bobot badan berkorelasi secara signifikan (p<0,05) dengan volume (r=0,317) dan motilitas individu (r=0,229), tetapi tidak signifikan (p>0,05) terhadap konsentrasi semen (r=0,046). Hasil penelitian secara keseluruhan menunjukkan sapi dengan bobot badan tinggi memiliki nilai kualitas semen yang lebih baik.
Hubungan Antara Umur, Bobot Badan, Lingkar Skrotum Dengan Produksi Spermatozoa Sapi Peranakan Ongole Chairdin Dwi Nugraha; Rafika Febriani Putri; Ahmad Furqon; Wike Andre Septian; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.3

Abstract

Sapi peranakan Ongole atau lebih dikenal dengan sapi PO merupakan salah satu ras sapi lokal yang banyak dipelihara di Indonesia. Pengembangan pada aspek reproduksi dalam pemilihan pejantan unggul menjadi sangat penting. Umur, bobot badan (BB), lingkar skrotum (LS) dan produksi semen merupakan kriteria dalam pemilihan pejantan unggul. penelitian ini bertujuan untuk mengetahui nilai korelasi antara umur, bobot badan dan lingkar skrotum terhadap total spermatozoa (TS) dan total spermatozoa motil (TSM). 11 ekor pejantan sapi PO di UPT PT & HMT Tuban dengan umur 2-8 tahun digunakan dalam penelitian ini. Total 48 ejakulasi dikoleksi selama empat minggu. Metode penelitian adalah observasional. Data dianalisis menggunakan SPSS 24 dengan metode OneWay ANOVA serta hubungan antar variabel menggunakan korelasi pearson (r). Hasil Penelitian menunjukkan bahwa umur berpengaruh nyata (p<0,05) terhadap nilai TS dan TSM. Umur, BB dan LS berkorelasi signifikan (p<0,05) terhadap TS dengan nilai masing masing secara berurutan yaitu (r=0,444), (r=0,615) dan (r=0,404). Umur, BB dan LS berkorelasi signifikan (p<0,05) terhadap TSM dengan nilai (r=0,459), (r=0,635) dan (r=0,402). Pemilihan pejantan unggul sapi PO perlu mempertimbangkan BB, umur dan LS yang tinggi sesuai Standar Nasional Indonesia.
The Effect of Different Breeds and Ages on Semen Production at Singosari National Insemination Center Ahmad Furqon; Irida Novianti; Wike Andre Septian; Rafika Febriani Putri; Chairdin Dwi Nugraha; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 2 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The study was aimed to evaluate semen production on different levels of breeds and ages. A total of 28 bulls were used in this study constisted of 13 Bali bulls, 6 Simmental bulls, and 9 Ongole Grade bulls. The bull’s age was ranged from 2 to 5 years old. The semen was collected at Singosari National Artificial Insemination Center three times a week during 2018. Bull’s semen was evaluated macroscopically and microscopically. Parameters analyzed in this study were semen volume, sperm motility and concentration. The result showed that the different semen quality was found among the bulls at different ages. Semen volume increased with time until 5 years genarally. Bali cattle had the lowest sperm concentration. Ongole Grade cattle had higher sperm motility and lower semen volume. Simmental had the lowest sperm motility. Simmental cattle had semen volume more than 6 mL. In conclusion, Ongole Grade cattle have higher semen production than Bali and Simmental. The failed semen collection was found among the bulls but the qualified semen production based on SNI would be used in the process of frozen semen at Singosari National Artificial Insemination Center
Analisis Hubungan Bobot Badan Terhadap Produksi Semen Sapi Limousin Di Balai Besar Inseminasi Buatan-Singosari Gama Yusaq Syah Alam; Enniek Herwijanti; Irida Novianti; Ahmad Furqon; Wike Andre Septian; Woro Busono; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 21, No 2 (2020): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2020.021.02.6

Abstract

Penelitian ini bertujuan untuk menganalisis hubungan bobot badan dengan kualitas semen. Materi yang digunakan yaitu data sekunder mulai tahun 2012 sampai 2018 dari 29 ekor pejantan sapi Limousin meliputi data kualitas semen sebanyak (n=27257) ejakulasi. Tempat penelitian dilakukan di Balai Besar Inseminasi Buatan, Singosari, Jawa Timur. Metode penelitian adalah studi kasus. Variabel yang diamati yaitu bobot badan, volume semen, mortilitas individu dan konsentrasi semen. Data dianalisa dengan menggunakan SPSS 21 dengan metode One-Way ANOVA. Uji korelasi menggunakan korelasi Pearson (r) dan analisis statistik model regresi linier untuk menduga kualitas semen berdasarkan bobot badan. Hasil penelitian menunjukkan bahwa bobot badan sapi Limousin berbeda nyata (p<0,05) terhadap kualitas semen. Semakin tinggi bobot badan maka nilai motilitas dan konsentrasi menurun sedangkan volume semen meningkat. Bobot badan berkorelasi nyata (p<0,05) dengan volume (r=0,054), motilitas individu (r=0,096) dan konsentrasi semen (r=0,244). Disimpulkan bahwa bobot badan berpengaruh nyata tehadap volume, motilitas individu dan konsentrasi semen. Bobot badan memberikan korelasi negatif terhadap motilitas dan konsentrasi semen, namun memberikan korelasi positif terhadap volume semen.
Carcass And Wholesalecut Production Of Brahman Cross (BX) Heifer Kuswati Kuswati; Abdul Muhaimin; Wike Andre Septian; Trinil Susilawati
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 17 No. 3 (2022)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2022.017.03.7

Abstract

This research aims to determine the quantitative characteristics of Brahman cross heifer cattle's carcass and wholesale cut at different age levels. The number of heifer cattle taken as samples was 104 heifer. The research method used is a case study with the selection of locations by purpose sampling. Direct observation is used to obtain information on age, slaughter weight, carcass weight, wholesale cut weight, and parts thereof (primary, secondary, and manufacturing cut). The data obtained were then analyzed by analysis of variance (ANOVA). If there was a significant difference, it is followed by a further test of the Least Significant Difference (LSD). The results showed that the age levels in slaughter had a significant effect (P<0.05) on the slaughter weight, fresh carcass weight and wilted carcass weight, but had no effect on the percentage of carcass and wholesale cut. The older the age of cattle and the longer the cattle are raised, the higher their body weight will affect the slaughter weight produced, and the higher the slaughter weight, the higher the carcass weight made. Average slaughter weight at different age by Permanent Incisor (PI): PI0 (<18 months), PI2 (18-24 months) and PI4 (24- 36 months). While the average percentage of heifer carcass at different age levels was relatively the same (52,71% : 53,04% : 53.10%). The weight and rate of wholesale cut and its parts are also somewhat the same. The research results show that the slaughter age affects the slaughter weight and carcass weight but not dressing percentage and wholesale cut weight. Brahman cross heifer cattle at PI4 produced optimal carcass weight and wholesale cut.
Body and Head Morphometric of Kacang Goat under Semi Intensive Management at Fishpond Area in Sidoarjo Regency, Indonesia Inggita Leli Murtika; Desinta Wulandari; Fitria Ella Wardani; Ahmad Furqon; Wike Andre Septian; Tri Eko Susilorini; Suyadi Suyadi
ANIMAL PRODUCTION Vol. 24 No. 3 (2022)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2022.24.3.174

Abstract

Morphometric characterization is the basis of conservation strategies, performance improvement, breeding, and sustainable utilization plan. The purpose of this research was to determine the morphometrics of the Kacang Goats at the fishpond area in Sidoarjo Regency based on different ages and sex. The fishpond area in Sidoarjo is a closed area where goats are raised and have the potential for inbreeding with the consequence of smaller-sized offspring than normal. Morphometric measurements included the head, ears, neck, body, legs, tail, and horns. This research used a descriptive analysis approach, presenting data in form of mean, standard deviation, and coefficient of variation. The result showed that the average face length of male vs. female Kacang goats was 16.73 ± 2.40 cm vs. 19.68 ± 2.44 cm; with a face width of 11.25 ± 2.91 cm vs. 19.52 ± 3.18 cm; ear length of 14.62 ± 1.38 cm vs. 16.46 ± 1.47 cm; neck length of 15.53 ± 2.65 cm vs. 15.35 ± 2.14 cm; horn length of 7.41 ± 4.10 cm vs. 6.59 ± 2.89 cm; chest circumference of 59.33 ± 8.18 cm vs. 67.80 ± 6.32 cm; body length of 51.45 ± 6.70 cm vs. 56.91 ± 6.54 cm; body height of 54.41 ± 7.22 cm vs. 62.01 ± 5.53 cm; tail length of 13.21 ± 2.01 cm vs. 14.77 ± 3.37 cm; hind leg length of 39.20 ± 5.26 cm vs. 47.74 ± 5.47 cm; and front leg length of 37.00 ± 8.84 cm vs. 44.63 ± 8.65 cm. This research concludes that Kacang Goats in Sidoarjo has characteristics according to SNI standard, and the morphometric size was larger in female than male goats.