Irida Novianti
Dosen Fakultas Peternakan Universitas Brawijaya, Jalan Veteran, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur, Indonesia 65145

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Effect of breeds on semen characteristics of aged bulls in the Indonesian National Artificial Insemination Center Novianti, Irida; Purwantara, Bambang; Herwijanti, Enniek; Nugraha, Chairdin Dwi; Putri, Rafika Febriani; Furqon, Ahmad; Septian, Wike Andre; Rahayu, Sri; Nurgiartiningsih, Veronica Margareta Ani; Suyadi, Suyadi
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 2 (2020): August 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.02.10

Abstract

Madura and Bali cattle are two of Indonesian indigenous breeds. One possible method to increase their productivity is through artificial insemination (AI) using frozen semen. This study conducted at the Singosari National Artificial Insemination Center, East Java, Indonesia, to evaluate the semen characteristics of different bull breeds ranging in age from 7 to 10 years and to provide useful recommendations regarding the efficacy of older cattle. A total of 2016 ejaculation data recorded from Indonesian indigenous cattle (Madura and Bali bulls) and Bos taurus bulls (Simmental) were used in this study to analyze semen volume, sperm motility, and sperm concentration. Results revealed differences in semen volume, sperm motility, and sperm concentration among bull breeds (p < 0.05). Simmental bulls have the highest semen volume (p < 0.05) compared with Bali and Madura bulls. Base on sperm motility, Bali bulls showed the highest average value (p < 0.05) compared with Simmental and Madura bulls. Sperm concentration was the highest (p < 0.05) among Bali bulls compared with Simmental and Madura bulls. Another important finding was that the average semen volume, sperm motility, and sperm concentration of older bulls fulfilled the standard criteria established for frozen semen processing. Therefore, it would be possible to improve the performance of these breeds through artificial insemination using frozen semen of Madura and Bali bulls, which can be more widely distributed to farmers to increase their herd productivity. This study also indicates that Indonesian indigenous bulls have superior semen characteristics than Simmental bulls.
Single Nucleotide Polymorphims (snps) Identification of Inhibin Sub Unit-α (inha) Gene on Madura Bulls Irida Novianti; Chairdin Dwi Nugraha; Rafika Febriani Putri; Ahmad Furqon; Wike Andre Septian; S. Rahayu; V. M. A. Nurgiartiningsih; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.10

Abstract

INHA gene is a gene that is suggested to have role in reproductive system. Single Nucleotide Polymorphisms (SNPs) of Madura Bulls were identified in this study. Polymerase Chain Reactions (PCR) was used to amplify INHA gene region and MEGA 7 program was utilized to align the amplified region sequences with sequence from Ensembl database. Four SNPs found in INHA and they are located at the first exon. Two SNPs were misssense mutations that causing the substitution of amino acid leucine21 by proline, and amino acid valine63 by methionine and the other two SNPs were synonymous mutation. One of the synonymous SNPs was a novel mutation. Based on those identified SNPs, they could be suggested as potential candidate markers of reproduction traits for Madura bulls. Moreover, through heterozygosity value from the observed bulls, it was indicated that the genotype was varied in population. Therefore a molecular selection program could be designed to determine the Madura superior bull.
Analisis Hubungan Bobot Badan Terhadap Produksi Semen Segar Sapi Bali di Balai Besar Inseminasi Buatan-Singosari Chairdin Dwi Nugraha; Enniek Herwijanti; Irida Novianti; Ahmad Furqon; Wike Andre Septian; Woro Busono; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 20, No 1 (2019): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2019.020.01.9

Abstract

Sapi asli indonesia dengan populasi terbesar dibandingkan sapi lokal lainnya yaitu sapi Bali. Pengembangan sapi Bali secara berkelanjutan dengan cara meningkatkan kualitas genetik melalui aspek reproduksi dalam pemilihan pejantan unggul. Bobot badan adalah salah satu kriteria dalam memilih pejantan unggul. Penelitian ini bertujuan untuk menganalisis hubungan bobot badan dengan kualitas semen. Materi yang digunakan yaitu data sekunder mulai tahun 2012-2018 dari 50 ekor pejantan sapi Bali meliputi data kualitas semen sebanyak (n=1204) ejakulasi di Balai Besar Inseminasi Buatan, Singosari, Jawa Timur, Indonesia. Metode penelitian adalah studi kasus. Variabel yang diamati yaitu bobot badan, volume semen, motilitas individu dan konsentrasi semen. Data dianalisa dengan menggunakan SPSS 24 dengan metode One-Way ANOVA. Uji korelasi menggunakan korelasi Pearson (r) dan analisis statistik model regresi linier untuk menduga kualitas semen berdasarkan bobot badan. Hasil penelitian menunjukkan bahwa bobot badan sapi Bali mempengaruhi secara signifikan (p<0,05) terhadap kualitas semen. Semakin tinggi bobot badan maka nilai volume dan motilitas meningkat kecuali konsentrasi semen yang sangat fluktuatif. Bobot badan berkorelasi secara signifikan (p<0,05) dengan volume (r=0,317) dan motilitas individu (r=0,229), tetapi tidak signifikan (p>0,05) terhadap konsentrasi semen (r=0,046). Hasil penelitian secara keseluruhan menunjukkan sapi dengan bobot badan tinggi memiliki nilai kualitas semen yang lebih baik.
BEEF CATTLE MUSCULARITY CANDIDATE GENES Irida Novianti; Waynes S. Pitchford; Cynthia D. K. Bottema
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 20, No 1 (2010)
Publisher : Faculty of Animal Science, Universitas Brawijaya

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Abstract

Muscularity is a potential indicator for the selection of more productive cattle. Mapping quantitative trait loci (QTL) for traits related to muscularity is useful to identify the genomic regions where the genes affecting muscularity reside. QTL analysis from a Limousin-Jersey double backcross herd was conducted using QTL Express software with cohort and breed as the fixed effects. Nine QTL suggested to have an association with muscularity were identified on cattle chromosomes BTA 1, 2, 3, 4, 5, 8, 12, 14 and 17. The myostatin gene is located at the centromeric end of chromosome 2 and not surprisingly, the Limousin myostatin F94L variant accounted for the QTL on BTA2. However, when the myostatin F94L genotype was included as an additional fixed effect, the QTL on BTA17 was also no longer significant. This result suggests that there may be gene(s) that have epistatic effects with myostatin located on cattle chromosome 17. Based on the position of the QTL in base pairs, all the genes that reside in the region were determined using the Ensembl data base (www.ensembl.org). There were two potential candidate genes residing within these QTL regions were selected. They were Smad nuclear interacting protein 1 (SNIP1) and similar to follistatin-like 5 (FSTL5). (JIIPB 2010 Vol 20 No 1: 1-10)
The Effect of Different Breeds and Ages on Semen Production at Singosari National Insemination Center Ahmad Furqon; Irida Novianti; Wike Andre Septian; Rafika Febriani Putri; Chairdin Dwi Nugraha; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 2 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

The study was aimed to evaluate semen production on different levels of breeds and ages. A total of 28 bulls were used in this study constisted of 13 Bali bulls, 6 Simmental bulls, and 9 Ongole Grade bulls. The bull’s age was ranged from 2 to 5 years old. The semen was collected at Singosari National Artificial Insemination Center three times a week during 2018. Bull’s semen was evaluated macroscopically and microscopically. Parameters analyzed in this study were semen volume, sperm motility and concentration. The result showed that the different semen quality was found among the bulls at different ages. Semen volume increased with time until 5 years genarally. Bali cattle had the lowest sperm concentration. Ongole Grade cattle had higher sperm motility and lower semen volume. Simmental had the lowest sperm motility. Simmental cattle had semen volume more than 6 mL. In conclusion, Ongole Grade cattle have higher semen production than Bali and Simmental. The failed semen collection was found among the bulls but the qualified semen production based on SNI would be used in the process of frozen semen at Singosari National Artificial Insemination Center
Analisis Hubungan Bobot Badan Terhadap Produksi Semen Sapi Limousin Di Balai Besar Inseminasi Buatan-Singosari Gama Yusaq Syah Alam; Enniek Herwijanti; Irida Novianti; Ahmad Furqon; Wike Andre Septian; Woro Busono; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 21, No 2 (2020): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2020.021.02.6

Abstract

Penelitian ini bertujuan untuk menganalisis hubungan bobot badan dengan kualitas semen. Materi yang digunakan yaitu data sekunder mulai tahun 2012 sampai 2018 dari 29 ekor pejantan sapi Limousin meliputi data kualitas semen sebanyak (n=27257) ejakulasi. Tempat penelitian dilakukan di Balai Besar Inseminasi Buatan, Singosari, Jawa Timur. Metode penelitian adalah studi kasus. Variabel yang diamati yaitu bobot badan, volume semen, mortilitas individu dan konsentrasi semen. Data dianalisa dengan menggunakan SPSS 21 dengan metode One-Way ANOVA. Uji korelasi menggunakan korelasi Pearson (r) dan analisis statistik model regresi linier untuk menduga kualitas semen berdasarkan bobot badan. Hasil penelitian menunjukkan bahwa bobot badan sapi Limousin berbeda nyata (p<0,05) terhadap kualitas semen. Semakin tinggi bobot badan maka nilai motilitas dan konsentrasi menurun sedangkan volume semen meningkat. Bobot badan berkorelasi nyata (p<0,05) dengan volume (r=0,054), motilitas individu (r=0,096) dan konsentrasi semen (r=0,244). Disimpulkan bahwa bobot badan berpengaruh nyata tehadap volume, motilitas individu dan konsentrasi semen. Bobot badan memberikan korelasi negatif terhadap motilitas dan konsentrasi semen, namun memberikan korelasi positif terhadap volume semen.
Novel Mutations in The Coding Region of The Third Exon of Myostatin (MSTN) Gene in Madura Cattle irida novianti; Rafika Febriani Putri; Doni Herviyanto; Ahmad Furqon; Wike Andre Septian; Kuswati Kuswati; suyadi suyadi
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 1 (2023): April 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.01.08

Abstract

Molecular information using gene variants could support livestock selection to improve livestock productivity. This study aimed to identify the variants in part of the MSTN gene (part of the third exon coding region), a gene known for its significant effect on muscle growth. DNA was isolated from 95 Madura cattle blood samples collected in Pamekasan, Madura. PCR was used to amplify to MSTN region in part of the third exon, and one-way sequencing was conducted to obtain the sequence from the amplified product. Six SNPs were identified. Four SNPs were novel mutations, and the other two SNPs were recorded in the Ensembl database (rs466598800 and rs211583837). Among the identified novel mutations, one mutation in c.1301G/T caused a stop codon for the TT genotype cattle. However, no cattle carried the TT genotype. Three other mutations in c.1324, c.1373, and c.1399 were missense mutations that change the amino acids Q311L, A318P, and L335B, respectively. Most of the cattle had heterozygote genotypes for all the identified SNPs. It could also be noted that there were sequence differences in the part of the third exon identified between Madura cattle and Zebu cattle (by Tantia et al., 2006).
MANAJEMEN KANDANG TERNAK UNTUK MENINGKATKAN KESEHATAN DAN HASIL PRODUKSI TERNAK DESA BULUSAN KECAMATAN KALIPURO KABUPATEN BANYUWANGI JAWA TIMUR Hasibuan, Albar Adetary; Rahman, Khalid; Wafi, In’amul; Novianti, Irida
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 6 No. 2 (2025): Volume 6 No. 2 Tahun 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v6i2.43952

Abstract

Ketahanan pangan dan gizi masyarakat Indonesia sangat menjadi perhatian lebih-lebih sejak adanya program pemerintah makan siang. Ketercukupan pasokan daging dan susu merupakan hal utama yang perlu diperhatikan untuk memenuhi protein hewani bagi siswa-siswa di Indonesia. Dalam penyelenggaraan pengabdian ini, tim membidik sasaran program pengabdian pada bidang peternakan sebagai piranti penting meningkatkan gizi masyarakat dengan ketersediaan protein hewani agar ibu hamil dan anak-anak sekolah tidak mengalami kekurangan gizi untuk mendorong terciptanya generasi cerdas dan sehat. Tim pengabdian dengan pemerintah desa Bulusan kecamatan Kalipuro kabupaten Banyuwangi, membangun sinergitas dan diseminasi ilmu pengetahuan bersama kelompok ternak yang ada di desa Bulusan. Minimnya pengetahuan peternak terkait manajemen kandang untuk hewan ternak menjadi perhatian serius. Pengabdian masyarakat ini sebagai dorongan implementasi program pemerintah yang berkelanjutan yaitu SDGs (Sustainable Development Goals). Di desa Bulusan terdapat 741 orang peternak, dengan jumlah komoditas ternak sementara Sapi 376 ekor, kambing 222 ekor dan ayam 1.049 ekor. Jumlah tersebut masih sangat minim jika dibandingkan dengan kebutuhan asupan daging untuk masyarakat desa Bulusan yang berjumlah 8.035 jiwa. Solusi yang mampu diberikan adalah menggenjot produktivitas hewan ternak dengan pengetahuan modern yang melibatkan perguruan tinggi