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All Journal Journal of Sustainability Science and Technology
Farhan Baehaki
Institut Kesehatan Rajawali, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Energy

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Liver histology of Wistar rats (Rattus norvegicus) following oral administration of 50% ethanol Liah Kodariah; Rahmad Rezaldy; Suryanata Kesuma; Farhan Baehaki
Journal of Sustainability Science and Technology Vol. 2 No. 1 (2022): Journal of Sustainability Science and Technology (JOSST)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v2i1.21

Abstract

Ethanol, also known as ethyl alcohol, pure alcohol, and alcohol, is a toxic, volatile, flammable, and colourless liquid. Alcohol is the most commonly consumed alcoholic beverage in everyday life. EtOH is a common abbreviation for ethanol, where "Et" stands for the ethyl group (C2H5). Sugar fermentation to ethanol is one of the earliest organic reactions ever performed by humans; ethanol consumption has also been known for a very long time. This study aims to determine the histology of the liver in Wistar rats (Rattus norvegicus) by orally administering ethanol at a concentration of 50 percent. The method used in this study was an experimental study by looking at the histology of rat liver. Rats were divided into two groups, with seven rats each. Group 1 was given 50% ethanol orally for seven days, and group 2, as a control, was only given orally with distilled water. After seven days, the rats were slaughtered, and their livers were extracted for further histological preparations. In the histology results of group 1, the histological images of the livers of the rats P1, P2, P3, P4, P5, P6 and P7 were abnormal or damaged. In the presence of necrotic cells, oral administration of 50 percent alcohol causes damage to hepatocyte cells, as determined by the study's findings. However, in general, hepatocyte cell damage in the liver produces a score of 1.7, which indicates a change leading to cell damage.
Analysis of Rhodamine B content in shrimp paste at Ciroyom Market, Bandung City, West Java, Indonesia Robby Gus Mahardika; Farhan Baehaki; Heru Agung Saputra; Septiani Yusuf
Journal of Sustainability Science and Technology Vol. 2 No. 1 (2022): Journal of Sustainability Science and Technology (JOSST)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v2i1.22

Abstract

According to regulation number 722/MENKES/Per/IX/88 of the Minister of Health of the Republic of Indonesia, Rhodamine B is one of the dangerous dyes, and its use in food products is prohibited. Rhodamine B can cause adverse health effects including irritation, impaired liver function, and liver cancer. Although it has been banned, the use of Rhodamine B is still prevalent in the community's food supply, including shrimp paste. This can be observed in some of the shrimp pastes sold at the Ciroyom market in Bandung, which are bright red, uneven, and comprised of lumps. This study aims to determine the Rhodamine B content of shrimp paste sold at Ciroyom Market in Bandung, West Java, Indonesia. This research is descriptive in nature and employed a total sampling technique. Rhodamine B was analyzed in ten samples of commercially available red shrimp paste using a UV-Vis Spectrophotometer with a 565 nm wavelength. The results indicate that Rhodamine B was found in all samples, with concentrations ranging from 1.79 ppm to 3.999 ppm. Therefore, it can be concluded that widespread abuse of Rhodamine B in food ingredients persists among the residents of Bandung City, West Java, Indonesia.
Co-Authors Heru Agung Saputra Kesuma, Suryanata Liah Kodariah Rahmad Rezaldy Robby Gus Mahardika Septiani Yusuf