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All Journal Jurnal Veteriner Biology, Medicine, & Natural Product Chemistry Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Indonesian Journal of Natural Science Education Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Elfred Rinaldo Kasimo
Universitas Kadiri
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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EFEK PREVENTIF EKSTRAK DAUN KENTUT (Paederia foetida L.) TERHADAP KADAR INTERLEUKIN-6 PADA MENCIT MODEL SEPSIS YANG DIINDUKSI Escherichia coli Lisa Savitri; Elfred Rinaldo Kasimo
Indonesian Journal of Natural Science Education Vol 5, No 2 (2022): November
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/nse.v5i2.2778

Abstract

Sepsis adalah keadaan darurat medis yang menggambarkan respons imunologis sistemik tubuh dan perubahan nukleotida pada gen yang mengkodekan interleukin-6 (IL-6) yang menghasilkan polimorfisme sehingga meningkatkan faktor risiko kematian karena sepsis. Terapi berbasis imunoterapi yang ditargetkan sebagian besar belum terbukti efektivitasnya sejauh ini. Sehingga, diperlukan tindakan yang lebih mengarah pada tindakan preventif, salah satunya dengan daun kentut (Paederia foetida L.). Mencit yang telah diadaptasikan diberikan perlakuan selama 14 hari dengan variasi sebagai berikut: 1) kelompok mencit yang tidak diberikan sonde lambung (N), 2) kelompok mencit diberikan aquades, 3) kelompok mencit diberikan ciprofloxacin, 4) kelompok diberikan ekstrak daun kentut dengan dosis 100 mg/kgBB, 5) kelompokmencit diberikan ekstrak daun kentut dengan dosis 300 mg/kbBB, 6) kelompok mencit diberikan ekstrak daun kentut dengan dosis 500 mg/kgBB. Mencit yang telah diberikan perlakuan diinjeksi pada bagian peritoniumnya dengan E. coli dengan dosis 1x105 CFU/mL. Mencit setelah 24 jam pasca pemaparan polimikroba sepsis akan memperlihatkan kejadian apoptosis. Berdasarkan hasil ELISA didapatkan kadar IL-6 N sebesar 1625,83 pg/mL, K- sebesar 2656,17 pg/mL, K+ sebesar 2033,21 pg/mL, P1 sebesar 2272,67 pg/mL, P2 sebesar 2175,83 pg/mL, P3 sebesar  2064,83 pg/mL. Mekanisme antiinflamasi pada sepsis diduga disebabkan adanya kandungan saponin, flavonoid, dan minyak atsiri yang terdapat di dalam ekstrak daun kentut. Mekanisme antiinflamasi yang paling mungkin adalah diduga saponin mampu berinteraksi dengan banyak membran lipid, seperti fosfolipid yang merupakan prekursor prostaglandin dan mediator inflamasi lainnya.
PENGGALAKAN KEBIASAAN HIDUP BERSIH DAN SEHAT SEBAGAI UPAYA PENCEGAHAH PENYEBARAN COVID-19 DI KELURAHAN TAMANAN, KOTA KEDIRI Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Abdimas Ilmiah Citra Bakti Vol 4 No 2 (2023)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i2.1598

Abstract

Wabah COVID-19 telah menjadi pandemi global yang memengaruhi banyak negara, termasuk Indonesia. Pemerintah dan lembaga kesehatan telah melaksanakan berbagai langkah untuk mencegah penyebaran COVID-19. Salah satu langkah penting adalah menggalakkan kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri. Pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman masyarakat tentang kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19. Metode yang digunakan dalam pengabdian masyarakat ini adalah pendekatan partisipatif dengan melibatkan masyarakat secara aktif dalam pengumpulan data dan pelaksanaan kegiatan. Kegiatan yang dilakukan meliputi penyuluhan, demonstrasi, dan penyediaan fasilitas sanitasi. Aktivitas ini diikuti oleh 50 ibu rumah tangga selama 3 bulan, terdiri dari tiga kegiatan utama penyuluhan kesehatan tentang pola hidup bersih dan sehat untuk mencegah penularan COVID-19. Data untuk penilaian kebiasaan hidup sehat menggunakan instrumen kuesioner dan checklist sebelum dan sesudah pelatihan. Pengetahuan, sikap dan psikomotor ibu rumah tangga dalam penyebaran penularan COVID-19 setelah diberikan pelatihan kebiasaan hidup sehat selama 3 bulan mengalami peningkatan. Penilaian dilakukan dengan membandingkan hasil pre-test dan post-test pelatihan peserta. Setelah mengikuti pelatihan, sebagian besar kemampuan peserta masuk kategori tinggi pada aspek pengetahuan (76%), aspek sikap (82%) dan aspek aspek psikomotor (92%). Berdasarkan hasil pengabdian ini, dapat disimpulkan bahwa penggalakan kebiasaan hidup bersih dan sehat dapat menjadi upaya efektif dalam pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri.
PENGGALAKAN KEBIASAAN HIDUP BERSIH DAN SEHAT SEBAGAI UPAYA PENCEGAHAH PENYEBARAN COVID-19 DI KELURAHAN TAMANAN, KOTA KEDIRI Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Abdimas Ilmiah Citra Bakti Vol. 4 No. 2 (2023)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i2.1598

Abstract

Wabah COVID-19 telah menjadi pandemi global yang memengaruhi banyak negara, termasuk Indonesia. Pemerintah dan lembaga kesehatan telah melaksanakan berbagai langkah untuk mencegah penyebaran COVID-19. Salah satu langkah penting adalah menggalakkan kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri. Pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman masyarakat tentang kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19. Metode yang digunakan dalam pengabdian masyarakat ini adalah pendekatan partisipatif dengan melibatkan masyarakat secara aktif dalam pengumpulan data dan pelaksanaan kegiatan. Kegiatan yang dilakukan meliputi penyuluhan, demonstrasi, dan penyediaan fasilitas sanitasi. Aktivitas ini diikuti oleh 50 ibu rumah tangga selama 3 bulan, terdiri dari tiga kegiatan utama penyuluhan kesehatan tentang pola hidup bersih dan sehat untuk mencegah penularan COVID-19. Data untuk penilaian kebiasaan hidup sehat menggunakan instrumen kuesioner dan checklist sebelum dan sesudah pelatihan. Pengetahuan, sikap dan psikomotor ibu rumah tangga dalam penyebaran penularan COVID-19 setelah diberikan pelatihan kebiasaan hidup sehat selama 3 bulan mengalami peningkatan. Penilaian dilakukan dengan membandingkan hasil pre-test dan post-test pelatihan peserta. Setelah mengikuti pelatihan, sebagian besar kemampuan peserta masuk kategori tinggi pada aspek pengetahuan (76%), aspek sikap (82%) dan aspek aspek psikomotor (92%). Berdasarkan hasil pengabdian ini, dapat disimpulkan bahwa penggalakan kebiasaan hidup bersih dan sehat dapat menjadi upaya efektif dalam pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri.
Pemberian Monosodium Glutamat Selama Masa Organogenesis Meningkatkan Perkembangan Embrio Cacat pada Mencit Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.328

Abstract

Monosodium glutamate (MSG) biasanya berbentuk kristal halus dan berwarna putih. Senyawa MSG dibuat melalui proses fermentasi dari bahan dasar pati (gandum) dan gula molasses (tetes tebu) yang diberi nama sebagai garam natrium dan asam glutamat. Pemberian MSG selama induk mencit bunting bersifat embriotoksik. Penelitian ini bertujuan untuk mengetahui efek toksik MSG pada perkembangan embrio selama masa organogenesis. Penelitian pemberian MSG terhadap mencit dimulai pada kebuntingan hari ketujuh yaitu pada tahap organogenesis. Perlakuan dosis MSG yang diberikan adalah dosis 528 mg/kg BB, 696 mg/kg BB, dan 872 mg/kg BB. Peubah yang diamati dalam penelitian ini adalah jumlah fetus hidup atau mati dalam rahim pada kebuntingan hari ke-18, di samping morfologi fetus, bobot fetus dan perkembangan tulang rangka fetus. Hasil penelitian menunjukkan bahwa pemberian MSG yang dilakukan secara terus menerus sejak kebuntingan hari ke-7 hingga 16 membuat MSG tersebut masuk ke dalam tubuh induk mencit yang tidak memiliki kemampuan untuk menetralisir dan mendetokfikasi senyawa-senyawa kimia sehingga terakumulasi pada embrio mencit. Zat-zat tersebut mencapai embrio melalui pembuluh darah dan memengaruhi perkembangan fetus mencit. Penelitian ini menunjukkan bahwa ada pengaruh MSG terhadap perkembangan fetus mencit (Mus musculus) selama periode organogenesis di antaranya yaitu kecacatan pada mata (mikropthalmia dan anoftalmia), hidrosefalus ringan, jumlah metakarpal, jumlah metatarsal, dan ruas cervikal vertebrae tidak sama panjang. Abnormalitas perkembangan fetus mencit selama periode organogenesis meningkat sejalan dengan peningkatan dosis MSG yang diberikan dan bervariasi antar perlakuan. Semakin tinggi dosis MSG yang diberikan pada induk mencit bunting, maka semakin sedikit jumlah fetus yang hidup. Dapat disimpulkan bahwa MSG memengaruhi perkembangan fetus mencit selama masa organogenesis.
In Silico Molecular Docking and ADMET Evaluation of Active Compounds from Acalypha indica L. Against the HER2 Breast Cancer Target Lisa Savitri; Kharisul Ihsan; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.45-51

Abstract

Breast cancer is one of the leading causes of cancer-related mortality in women worldwide, and overexpression of human epidermal growth factor receptor 2 (HER2) is associated with aggressive tumor progression, poor prognosis, and treatment resistance. Natural compounds are increasingly explored as safer anticancer candidates due to their structural diversity and lower toxicity profiles. Acalypha indica L., a traditional medicinal plant widely used in Asia, contains numerous phytochemicals with reported antioxidant and cytotoxic activities. This study investigates the binding affinity and pharmacokinetic potential of major A. indica phytochemicals against HER2 using in silico molecular docking and ADMET predictions. Twelve bioactive compounds were selected: quercetin, kaempferol, luteolin, rutin, isoquercitrin, caffeic acid, ferulic acid, esculetin, lupeol, beta-sitosterol, stigmasterol, and acalyphin. Docking was performed using AutoDock Vina against HER2 (PDB ID: 3PP0). Kaempferol (-10.2 kcal/mol), quercetin (-9.8 kcal/mol), and luteolin (-9.3 kcal/mol) showed the highest affinity, interacting strongly with key residues within the HER2 ATP-binding pocket. ADMET analysis indicated that kaempferol, quercetin, and luteolin possessed favorable oral bioavailability and safety characteristics. These findings suggest that A. indica contains promising HER2-targeting phytochemicals that warrant further investigation through in vitro and in vivo studies.
In Silico Study of the Antibacterial Activity of Acalypha indica L. Compounds Against Staphylococcus aureus DNA Gyrase Protein Lisa Savitri; Kharisul Ihsan; Elfred Rinaldo Kasimo
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.137-141

Abstract

Flavonoids from Acalypha indica exhibit potential antibacterial activity against Staphylococcus aureus, particularly through inhibition of DNA gyrase B. This study evaluated molecular interactions of mauritanin, acalyphin, quercetin, and kaempferol using in silico approaches, including molecular docking, molecular dynamics simulation, and ADMET prediction. Docking results showed that mauritanin had the lowest binding energy (-8.5 kcal/mol) and formed stable interactions with key residues in the active site, corroborated by 50 ns molecular dynamics simulations. Pharmacokinetic and drug-likeness predictions indicated that mauritanin and acalyphin had favorable profiles, with high gastrointestinal absorption and low toxicity risk. The other flavonoids showed higher permeability across the central nervous system, potentially beneficial for CNS-targeted therapies. These findings support mauritanin as a promising lead compound for novel antibacterial agent development, warranting further in vitro and in vivo validation.
Isolation and Identification of Dermatophytes from Toenails and Interdigital Spaces of Students Using Czapek Yeast Extract Agar Lisa Savitri; Kharisul Ihsan; Rochmad Krissanjaya; Elfred Rinaldo Kasimo; Novirma Yanti; Mochamad Hanif Hilmi
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.795-798

Abstract

Dermatophytosis remains a common superficial fungal infection affecting nails and interdigital spaces, with Trichophyton rubrum and T. mentagrophytes as the predominant etiologic agents. The recent emergence of terbinafine-resistant T. indotineae has raised new challenges in diagnosis and treatment. Although Sabouraud Dextrose Agar is the conventional medium for fungal culture, alternative media such as Czapek Yeast Extract Agar (CYA) may provide additional advantages for dermatophyte isolation and morphological identification. This study aimed to isolate and identify dermatophytes from toenails and interdigital spaces of university students using CYA medium and to evaluate its applicability in dermatophyte culture. A cross-sectional descriptive study was conducted among 100 university students aged 18–24 years. Specimens were collected from toenails (n = 60) and interdigital spaces (n = 40). Direct microscopy was performed using 20% KOH preparation. Samples were cultured on CYA supplemented with chloramphenicol and cycloheximide, and incubated at 28 ± 2 °C for up to 21 days. Fungal isolates were identified based on macroscopic and microscopic morphology. Data were analyzed descriptively, and associations were tested using the chi-square test. Fungal elements were detected in 65% of samples by KOH examination, and dermatophyte growth was confirmed in 52% of cultures. The most frequently isolated species were T. rubrum (28%) and T. mentagrophytes (18%). Notably, T. indotineae was detected in 5% of samples. No significant difference was found between toenail and interdigital isolates (p = 0.26). Dermatophytes are prevalent among university students, with T. rubrum as the dominant species. The detection of T. indotineae highlights its emerging role in young populations. CYA proved effective for dermatophyte isolation and may serve as an alternative culture medium in academic and diagnostic laboratories.
Onychomycosis in Students: An Overview of Nail Care Practices and Personal Hygiene Lisa Savitri; Kharisul Ihsan; Rochmad Krissanjaya; Elfred Rinaldo Kasimo; Novirma Yanti; Mochamad Hanif Hilmi
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1015-1019

Abstract

Onychomycosis is a common nail disorder caused by dermatophytes, yeasts, and non-dermatophyte molds, with increasing prevalence among young adults. Poor nail care and personal hygiene habits are essential predisposing factors that contribute to infection. Despite its clinical relevance, data on onychomycosis in student populations remain limited. A cross-sectional study was conducted among 150 undergraduate students from the Faculty of Health Sciences, aged 18–25 years. Nail specimens were collected from individuals presenting with suspected onychomycosis. Direct microscopy was performed using a 20% potassium hydroxide (KOH) preparation, and samples were cultured on Sabouraud Dextrose Agar supplemented with chloramphenicol and cycloheximide. Fungal isolates were identified based on macroscopic and microscopic characteristics. Sociodemographic data, nail care habits, and hygiene practices were collected through structured questionnaires. Data were analyzed descriptively, and associations were assessed using chi-square tests. Onychomycosis was confirmed in 27 students (18%). Dermatophytes were the most frequent isolates, with Trichophyton rubrum (9%) and T. mentagrophytes (5%) predominating, followed by Candida albicans (3%) and Aspergillus spp. (1%). Risk factors significantly associated with infection included irregular nail trimming (p = 0.01), sharing of nail clippers (p = 0.03), and prolonged use of closed footwear (p = 0.02). No significant associations were observed with gender or age. Onychomycosis is prevalent among university students, with dermatophytes as the leading causative agents. Inadequate nail care and poor hygiene practices play a critical role in the occurrence of diseases. Health education and preventive interventions should be prioritized to reduce infection rates in young adults. Further studies employing molecular methods are recommended to refine species identification and improve epidemiological insights.
The Influence of Strain Type and Female Age on the Receptivity of Female Drosophila melanogaster in Homogamous Crosses Lisa Savitri; Kharisul Ihsan; Rochmad Krissanjaya; Elfred Rinaldo Kasimo
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.937-941

Abstract

Drosophila melanogaster is a widely used model organism in genetic research due to its short life cycle and the presence of many genes homologous to those found in humans. It plays an important role in studies related to genetic inheritance, gene interaction, sex determination, and developmental genetics. One key aspect of its reproductive biology is female receptivity—the willingness of a female to accept mating—which is influenced by various internal and external factors. External factors include environmental conditions such as temperature and humidity, as well as male courtship signals like wing vibrations and chemical cues. Internal factors are mainly related to the female's sexual maturity and mating status. Generally, female receptivity increases with age after eclosion, reaches a peak, and gradually declines. This study investigates the impact of strain type and female age on receptivity in homogamous crosses of D. melanogaster, using two mutant strains: dp (with a wing mutation) and wa (with an eye color mutation). Receptivity was measured by the presence or absence of F1 offspring following crosses between males and females of the same strain, with females tested at different ages ranging from 10 to 60 hours after eclosion. The results indicate that strain type does not have a significant effect on female receptivity. In contrast, female age plays a clear role: individuals aged 30 years and older were more receptive and more likely to produce offspring. No significant interaction was found between strain type and age. These findings suggest that female receptivity in D. melanogaster is more strongly influenced by sexual maturity (as indicated by age) than by genetic differences between strains.
Academic Stress and Blood Glucose Levels: A Correlational Study Among Medical Laboratory Technology Students Lisa Savitri; Mochamad Hanif Hilmi; Elfred Rinaldo Kasimo; Rochmad Krissanjaya; Kharisul Ihsan
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.977-981

Abstract

Diabetes mellitus is a chronic metabolic disorder characterized by elevated blood glucose levels, often influenced by various physiological and psychological factors. One such factor is stress, particularly academic stress among university students. This study aims to explore the potential correlation between academic stress levels and random blood glucose levels among Medical Laboratory Technology (D.IV) students at Kadiri University. A cross-sectional, quantitative approach was used in this study. A total of 28 eligible students participated. Academic stress levels were assessed using the Perceived Sources of Academic Stress (PSAS) questionnaire, while random blood glucose levels were measured using spectrophotometric methods with glucose GOD FS reagents. Data were analyzed using the Pearson correlation test to determine the relationship between the two variables. The majority of students experienced moderate academic stress (71.43%), while the rest (28.57%) experienced high stress. No students reported low stress levels. Regarding blood glucose, 53.57% of participants had normal levels, and 46.43% had elevated levels. The Pearson correlation coefficient was 0.045 with a p-value of 0.819, indicating no statistically significant relationship between academic stress levels and blood glucose levels. Regression analysis also confirmed that while there was a slight positive trend (suggesting that higher stress may slightly raise glucose levels), the effect was negligible and not statistically significant. This study found no significant correlation between academic stress and random blood glucose levels among Medical Laboratory Technology students. Although stress may contribute to changes in glucose levels through hormonal mechanisms, other factors—such as diet, physical activity, and individual coping strategies—likely play a more substantial role. Interventions focusing on stress management, healthy lifestyle habits, and regular health monitoring are recommended to maintain both psychological well-being and metabolic health in students.
Co-Authors Aprilia Anjarwati Dita Apriana Dwi Astuti Kharisul Ihsan Mochamad Hanif Hilmi Novirma Yanti Rochmad Krissanjaya savitri, Lisa