Boedi Oetomo Roeslan, Boedi Oetomo
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Stenochlaena palustris Ethanol Extract Decreases Viability and Induces G1-Phase Cell Cycle Arrest in HSC-3 Tongue Cancer Cells via p21 and p27 Sandra, Ferry; Ranggaini, Dewi; Halim, Johni; Taramalinda, Elizabeth Yuliani; Scania, Alifah Evi; Roeslan, Boedi Oetomo; Lee, Kyung Hoon
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3308

Abstract

BACKGROUND: Oral squamous cell carcinoma (OSCC) of the tongue is an aggressive cancer with a poor prognosis due to its resistance to standard treatments. Stenochlaena palustris, a medicinal fern containing bioactive compounds, has shown potential anticancer properties. However, there is a lack of studies addressing the effects of S. palustris ethanol extract (SPEE) on tongue cancer. This study examined the effects of SPEE on the cell viability and cell cycle of human squamous cell carcinoma (HSC)-3 tongue cancer cells.METHODS: SPEE was prepared with the maceration method. HSC-3 cells were treated with SPEE at concentrations of 100, 500, and 1000 µg/mL for 24 and 48 hours. Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis was performed using flow cytometer. Immunoblotting was used to measure amount of cell cycle regulators, protein 21 (p21) and protein 27 (p27).RESULTS: SPEE treatment led to a significant decrease in HSC-3 viable cells in a concentration- and time-dependent manner, with the most pronounced effect at higher concentration and prolonged treatment time. There was a slightly increase in the percentage of cells in the Sub-G1 phase in SPEE-treated group, meanwhile there was a significant increase in the percentage of cells in the G1-phase. Increased amount of p21 and p27 were observed in SPEE-treated group.CONCLUSION: SPEE significantly inhibited HSC-3 cell proliferation in a concentration- and time-dependent manner, primarily by inducing G1-phase cell cycle arrest through the upregulation of p21 and p27. Taken together, SPEE could be a potential anti-cancer agent for tongue cancer cell. KEYWORDS: Stenochlaena palustris, tongue cancer, cytotoxic, cell cycle arrest, HSC-3 cells, p21, p27
Dentist: Wrist Position, Handgrip Strength, and Body Mass Index Associated with Carpal Tunnel Syndrome in Daily Practice Ariyani, Annisaa Putri; Anggraini, Wita; Roeslan, Boedi Oetomo; Sulistyowati, Indrani
Malahayati Nursing Journal Vol 6, No 9 (2024): Volume 6 Nomor 9 (2024)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v6i9.15525

Abstract

ABSTRACT Carpal tunnel syndrome (CTS) is one of the most common musculoskeletal disorders affecting dentists and their ability to work. Prolonged work with repetitive flexion and extension of the wrist position and high force may increase the risk of CTS. Exposure to various individual factors (gender, age, work duration, work experience, body mass index), wrist position, and handgrip strength during daily practice may also trigger CTS. To determine whether individual factors, wrist position, and handgrip strength were associated with CTS incidence in dentists. A case-control study in the population of the epidemiological study area. Dentists practicing at the community health center in Tangerang, Banten, agreed to complete the informed consent form. The total population of this present study in the forty-four community health centers in Tangerang was sixty-seven participants. Two participants were excluded due to a history of arthritis and diabetes mellitus. Therefore, only sixty-five participants who met the inclusion criteria were included. The assessment included observation of wrist position, handgrip strength, body mass index, and individual questionnaires (gender, age, work duration, and work experience). The association between these variables and CTS was then analyzed using the chi-squared test. The distribution of CTS among dentists showed that eighteen participants complained of CTS, while forty-seven participants did not. In a case group, the majority of participants complaining of work-related CTS symptoms were female (94.40%). We found that CTS sufferers were over 35 years of age (61.10%), had more than 9 years of work experience (61.10%), and had less than 6 hours of work duration (61.60%). Whereas, 100% of the participants performed wrist position at risk for CTS, 77.80% had weak handgrip strength, and 50% were obese based on body mass index compared to the control group at 80.90%, 4.30%, and 2.10%, respectively. The Chi-square test showed a significant level of wrist position, handgrip strength, and BMI against CTS. CTS is a disease associated with a dentist's wrist position, handgrip strength, and body mass index during dental practice. Keywords: Dentist, Carpal Tunnel Syndrome, Wrist Position, Handgrip Strength, Body Mass Index