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All Journal IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) The Indonesian Biomedical Journal Borneo Journal Of Pharmascientech Narra J JURNAL ABDI MASYARAKAT INDONESIA (JAMIN) Jurnal Abdimas Kesehatan Terpadu
Ranggaini, Dewi
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Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Penyuluhan dan Pelatihan Peningkatan Kemampuan Menjaga Kebersihan Gigi dan Mulut serta Tatalaksana ke Dokter Gigi selama Pandemi COVID-19 Ranggaini, Dewi; Sadono, Melanie; Halim, Himawan; N Santosa, Didi
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 2 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i2.21744

Abstract

COVID-19 cases in Indonesia have increased to require comprehensive efforts in case management and efforts to break the chain of transmission. The community in the RW. 07 Benteng Sub-district, Warudoyong District, Sukabumi City have oral health problems such as cavities, swelling of the gum, and bad breath. At the time of the COVID-19 pandemic, people are terrified to go to the dentist because of the information that dental care considers having a higher risk of COVID-19. Based on the above problems, the FKG Trisakti University’s Team provided education in counseling and training to the community in RW. 07 Sukabumi City. The case emergency, even though during the COVID-19 pandemic to get dental care, so as not to aggravate further the situation where unchecked, the swelling might interfere with breathing and endanger personal safety. they are also informed about the management to go to the dentist. To minimize the transmission of the virus such as always using a mask, carrying out the WHO standard 6-step hand washing procedure, sneezing or coughing ethics, and doing physical restrictions. While in the waiting room, also when in the practice room, the dentist and other health teams use Personal Protective Equipment (PPE), disinfection, cleaning, and handling of tools and the patient rinses his mouth with Povidone-iodine mouthwash before starting treatment. We can maintain Oral hygiene and teeth remain healthy if brushing teeth is doing appropriately, using dental floss, mouthwash from natural ingredients such as betel leaf, and avoiding foods that contain lots of sugar.
Apium graveolens leaf ethanolic extract triggers apoptosis in human tongue cancer cells via caspase-3 and poly(ADP-ribose) polymerase pathways: An in vitro study Sandra, Ferry; Hartono, Tiffany; Hayuningtyas, Ria A.; Ranggaini, Dewi; Halim, Johni; Lee, Kyung H.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1634

Abstract

Recent advances in cancer treatment have focused on developing alternative therapies with reduced adverse effects. Chemoprevention using natural products derived from plants has gained significant attention. Apium graveolens has demonstrated anticancer properties against various cancer cell types, suggesting its potential efficacy against tongue cancer cells. The aim of this study was to evaluate the cytotoxic effects and mechanisms of action of Apium graveolens leaf ethanolic extract (AGLEE) on the HSC-3 tongue cancer cell line. The leaves were processed and extracted with 70% ethanol to obtain an ethanolic extract. HSC-3 cells were cultured, subjected to starvation, and pre-treated with or without Z-DEVD-FMK, a caspase-3 inhibitor. Subsequently, the cells were treated with or without doxorubicin or varying concentrations of AGLEE. To assess cell viability and apoptosis, MTT and sub-G1 assays were performed. Additionally, treated HSC-3 cells were collected, lysed, and analyzed for levels of cleaved-caspase-3 and cleaved-poly (ADP-ribose) polymerase (cleaved-PARP) using ELISA. The inhibitory concentration (IC50) value of AGLEE for reducing viable HSC-3 cells was determined to be 48.29 μg/mL. AGLEE significantly decreased HSC-3 cell viability and increased the percentage of apoptotic cells. It exhibited a concentration-dependent reduction in cell viability and an increase in apoptosis. Furthermore, the extract elevated the levels of cleaved-caspase-3 and cleaved-PARP in HSC-3 cells. Pre-treatment with Z-DEVD-FMK reduced the levels of cleaved-caspase-3 and cleaved-PARP induced by AGLEE. Taken together, AGLEE could be proposed as a potential natural therapeutic agent by inducing apoptosis through the caspase-3/PARP pathway in tongue cancer cells.
PENYULUHAN GURU-GURU SEKOLAH MENENGAH TENTANG GANGGUAN MUSKULOSKELETAL PADA REMAJA Marpaung,SpPros.,PhD, Carolina Damayanti; Hanin, Isya; Anggraini, Wita; Juliawati, Mita; Ranggaini, Dewi; Krisna, Yohanes; Michelle, Michelle; Putri, Ni Luh
Jurnal Abdi Masyarakat Indonesia (JAMIN) Vol 6 No 2 (2024): JURNAL ABDI MASYARAKAT INDONESIA (JAMIN)
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jamin.v6i2.19187

Abstract

Musculoskeletal disorders are common issues that affect various age groups, including children and adolescents. In dentistry, a frequently encountered musculoskeletal disorder is temporomandibular disorder, which can significantly impact the quality of life of those affected. Schools play a crucial role in preventing and detecting musculoskeletal disorders among students. However, the literature indicates that teachers' knowledge regarding musculoskeletal disorders generally remains relatively low. Therefore, education and training programs must enhance teachers' understanding of these issues. As part of its commitment to health education, the Community Service Team (PkM) of Universitas Trisakti conducted an awareness program for 100 secondary school teachers at IPEKA Tomang. The program was carried out in two stages, focusing on general musculoskeletal and orofacial disorders. The evaluation results showed an improvement in teachers' understanding of both topics, which is expected to contribute to the prevention and early management of musculoskeletal disorders in schools. 
Inositol Hexakisphosphate (InsP₆) Induces Apoptosis via Caspase-Dependent Pathways: Molecular Docking Insights Sandra, Ferry; Ranggaini, Dewi; Halim, Johni; Pakpahan, Alfred; Pratitis, Visi Endah; Lee, Kyung Hoon
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3810

Abstract

BACKGROUND: Inositol hexakisphosphate (InsP₆) exhibits anticancer activity, especially by inducing intrinsic and extrinsic apoptotic pathways. However, there is still no molecular docking evidence that directly examines InsP₆ interactions with either upstream or downstream apoptotic regulators. Therefore, the current study was conducted to investigate the molecular docking of InsP₆ to caspases as upstream/downstream apoptotic regulators.METHODS: Ligands including InsP₆, InsP₅, InsP₄, histone deacetylase inhibitor, and caspase inhibitors were retrieved from PubChem, while target proteins (histone, caspase-8, caspase-2, and caspase-3) were obtained from the Protein Data Bank. Ligand toxicity was predicted using ProTox-3.0, and physicochemical properties were analyzed with SwissADME. Ligand structures were energy-minimized using PyRx with the Universal Force Field, while proteins were prepared by removing water molecules and non-essential heteroatoms in BIOVIA Discovery Studio. Molecular docking was conducted using CB-Dock 2.0, with binding poses selected based on the lowest Vina score, and ligand–protein interactions were visualized in Discovery Studio.RESULTS: Molecular docking results showed that InsP₆ bound strongly to histone, caspase-8, caspase-2, and caspase-3 with affinities comparable to reference inhibitors, forming multiple hydrogen bonds with key active-site residues. InsP₆, InsP₅, and InsP₄ exhibited several similar binding sites to caspase-3, with only minor differences in binding affinity.CONCLUSION: InsP₆ shows strong binding to histone, caspase-8, caspase-2, and caspase-3 based on in silico results, supporting its role in inducing both extrinsic and intrinsic apoptotic pathways. Taken together, InsP₆ could be a potential inducer of apoptosis in cancer cells.KEYWORDS: cancer, apoptosis, InsP₆, InsP₅, InsP₄, caspase, in silico, molecular docking
Efektivitas Enkapsulasi Nano Kitosan Serai Dapur dalam Meningkatkan Depolarisasi Mitokondria pada Sel Kanker HSC-3 Komariah, Komariah; Moksidy, Jenyfer Ignatia; Nugroho, Didi; Ranggaini, Dewi; Anggraeni , Rezky; Halim , Johni
BORNEO JOURNAL OF PHARMASCIENTECH Vol 9 No 2 (2025): Borneo Journal Of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59053/bjp.v9i2.602

Abstract

Oral cancer ranks 12th among the most common types of cancer worldwide, with a prevalence of approximately 25%-40% occurring on the lateral borders and the underside of the tongue. Conventional therapies often cause side effects that damage normal cells, prompting the use of safer natural alternatives, such as Cymbopogon citratus (lemongrass). The active compounds in lemongrass leaves can enhance the production of reactive oxygen species (ROS), which trigger apoptosis through the depolarization of mitochondrial membrane potential (MMP). To improve the stability of these active compounds, they are encapsulated with chitosan, which is modified into nanoparticles to enhance diffusion through cell membranes, thereby increasing their effectiveness as anticancer agents. This study involved nine groups, including a negative control, a positive control (doxorubicin), nano chitosan, lemongrass extract, and five treatment groups with various encapsulation concentrations (100%, 75%, 50%, 25%, and 12.5%). The results showed that nano chitosan encapsulation of lemongrass extract significantly increased MMP depolarization compared to the control. The depolarization effect increased with higher encapsulation concentrations, with the lowest red fluorescence intensity observed at the 100% concentration, indicating the highest level of depolarization in HSC-3 cells.
Elephantopus scaber Linn. Leaf Extract Sensitizes Doxorubicin in Inducing Apoptosis in HSC-3 Tongue Cancer Cells through Inhibiting Survivin Activity at Thr34 Sandra, Ferry; Hayuningtyas, Ria Aryani; Ranggaini, Dewi; Pang, Tiffany; Scania, Alifah Evi; Lee, Kyung Hoon
The Indonesian Biomedical Journal Vol 16, No 4 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i4.3096

Abstract

BACKGROUND: Previous research has demonstrated the effect of Elephantopus scaber Linn. leaf extract (ESLE) on various cancer cell lines. However, research on the effects of ESLE on oral squamous cell carcinoma (OSCC), especially tongue cancer, is still lacking. Moreover, the apoptotic mechanisms induced by ESLE are not well understood and require further exploration. Therefore, this study was conducted to investigate the effects of ESLE on cell viability and apoptosis in human squamous cell carcinoma (HSC)-3 tongue cancer cells.METHODS: HSC-3 cells were treated with varying concentrations of ESLE, doxorubicin, and a combination of both. Cell viability and apoptosis were assessed using MTT and Sub-G1 assays. The expression levels of survivin and its phosphorylated form at threonine (Thr)34 were evaluated using Western blot analysis.RESULTS: ESLE exhibited a concentration-dependent cytotoxic effect on HSC-3 cells in decreasing cell viability (Kruskal Wallis, p=0.001) and increasing apoptotic cells (ANOVA, p=0.001) significantly. When combined with doxorubicin, ESLE further enhanced the induction of apoptosis compared with doxorubicin alone. The combined treatment resulted in a decrease in the levels of phosphorylated survivin (p-Surv) Thr34, indicating the inhibition of survivin's anti-apoptotic function.CONCLUSION: ESLE significantly enhances the efficacy of doxorubicin, thereby sensitizing its ability to induce apoptosis in HSC-3 tongue cancer cells. This sensitization occurs through the inhibition of survivin activity, particularly at the Thr34 phosphorylation site. These findings suggest that ESLE could serve as a potential adjuvant to improve the effectiveness of doxorubicin in inducing apoptosis in tongue cancer cells.KEYWORDS: Elephantopus scaber, doxorubicin, tongue cancer, HSC-3 cells, apoptosis, Survivin, Thr34 phosphorylation
Stenochlaena palustris Ethanol Extract Decreases Viability and Induces G1-Phase Cell Cycle Arrest in HSC-3 Tongue Cancer Cells via p21 and p27 Sandra, Ferry; Ranggaini, Dewi; Halim, Johni; Taramalinda, Elizabeth Yuliani; Scania, Alifah Evi; Roeslan, Boedi Oetomo; Lee, Kyung Hoon
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3308

Abstract

BACKGROUND: Oral squamous cell carcinoma (OSCC) of the tongue is an aggressive cancer with a poor prognosis due to its resistance to standard treatments. Stenochlaena palustris, a medicinal fern containing bioactive compounds, has shown potential anticancer properties. However, there is a lack of studies addressing the effects of S. palustris ethanol extract (SPEE) on tongue cancer. This study examined the effects of SPEE on the cell viability and cell cycle of human squamous cell carcinoma (HSC)-3 tongue cancer cells.METHODS: SPEE was prepared with the maceration method. HSC-3 cells were treated with SPEE at concentrations of 100, 500, and 1000 µg/mL for 24 and 48 hours. Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis was performed using flow cytometer. Immunoblotting was used to measure amount of cell cycle regulators, protein 21 (p21) and protein 27 (p27).RESULTS: SPEE treatment led to a significant decrease in HSC-3 viable cells in a concentration- and time-dependent manner, with the most pronounced effect at higher concentration and prolonged treatment time. There was a slightly increase in the percentage of cells in the Sub-G1 phase in SPEE-treated group, meanwhile there was a significant increase in the percentage of cells in the G1-phase. Increased amount of p21 and p27 were observed in SPEE-treated group.CONCLUSION: SPEE significantly inhibited HSC-3 cell proliferation in a concentration- and time-dependent manner, primarily by inducing G1-phase cell cycle arrest through the upregulation of p21 and p27. Taken together, SPEE could be a potential anti-cancer agent for tongue cancer cell. KEYWORDS: Stenochlaena palustris, tongue cancer, cytotoxic, cell cycle arrest, HSC-3 cells, p21, p27
Curcuma xanthorrhiza Rhizome Extract Induces Apoptosis in HONE-1 Nasopharyngeal Cancer Cells Through Bid Ranggaini, Dewi; Sandra, Ferry; Halim, Johni; Ichwan, Solachuddin Jauhari Arief; Djamil, Melanie Sadono
The Indonesian Biomedical Journal Vol 15, No 1 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i1.2217

Abstract

BACKGROUND: Curcuma xanthorrhiza rhizomes have been demonstrated to have anticancer properties toward various types of cancer cells. The effect of C. xanthorrhiza rhizome extract (CXRE) on nasopharyngeal cancer (NPC) cells, including HONE-1 cell line has not been elucidated yet. Therefore, the effect of CXRE on the apoptosis of HONE-1 cells and its possible underlying mechanism are necessary to be explored.METHODS: C. xanthorrhiza rhizomes were minced, dried, extracted with distilled ethanol, filtered, and evaporated to produce CXRE. HONE-1 cells were seeded, starved, and treated with dimethyl sulfoxide (DMSO), Doxorubicin, or various concentrations of CXRE. Treated HONE-1 cells were stained with 4',6'-diamidino-2-phenylindole (DAPI) and the number of viable cells was counted. HONE-1 cells were also collected, lysed, and further processed for immunoblotting analysis to measure Bid activity.RESULTS: The number of viable HONE-1 cells decreased in concentration- and time-dependent manner. The number of viable cells in 50 and 250 μg/mL CXRE-treated groups were significantly lower compared with that in the DMSO-treated group after 24 h. At 48 h incubation period, the number of viable cells in 10, 50 and 250 μg/mL CXRE-treated groups were significantly lower compared with that in the DMSO-treated group. The number of viable cells in 250 μg/mL CXRE-treatment group was not significantly different compared with that in the Doxorubicin-treated group after 48 h. Bid expression levels in CXRE-treated groups were lower compared with that in the DMSO-treated group.CONCLUSION: CXRE could induce apoptosis via Bid activation, hence reducing the viability of HONE-1 cells.KEYWORDS: Curcuma xanthorrhiza, nasopharyngeal cancer, HONE-1 cells, apoptosis, Bid
Antibacterial Activity of Nanocomposite Chitosan-Silver Nanoparticle with Cymbopogon citratus Extract as a Bioreductor Against Staphylococcus aureus Anggraeni, Rezky; Melyda, Ananda Shelly; Pakpahan, Alfred; Ranggaini, Dewi; Halim, Johni; Komariah, Komariah
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 2 (2025): June 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v10.i2.112

Abstract

Nanocomposites are materials formed by combining two components, one or both of which are on the nanometer scale. The nanocomposite in this study is a combination of chitosan and silver nanoparticles produced through the synthesis of silver nitrate using Cymbopogon citratus extract. Silver nanoparticles have antibacterial abilities that can be utilized to overcome various diseases. However, their antibacterial properties may be reduced due to the tendency of silver nanoparticles to agglomerate. This can be overcome by the addition of chitosan as a stabilizing agent to prevent agglomeration and maintain the antibacterial effectiveness of silver nanoparticles. This study aims to evaluate the antibacterial activity of a nanocomposite formed by combining chitosan and silver nanoparticles synthesized using Cymbopogon citratus extract against Staphylococcus aureus through the diffusion method. The samples used included nanocomposites at concentrations of 6.25 mg/mL, 12.5 mg/mL, 15 mg/mL, 25 mg/mL, and 50 mg/mL, amoxicillin as a positive control, Acetic acid, and distilled water as negative controls. The results of antibacterial activity testing showed that all nanocomposite test concentrations had the ability to inhibit the growth of Staphylococcus aureus as evidenced by the formation of an inhibition zone around the disc paper. However, the highest antibacterial activity shown by the nanocomposites was still lower compared to the antibacterial activity of amoxicillin Keywords: Antibacterial activity, Chitosan, Cymbopogon citratus, Silver nanoparticles, Staphylococcus aureus
Co-Authors Alfred Pakpahan, Alfred Anggraeni , Rezky Anggraeni, Rezky Boedi Oetomo Roeslan, Boedi Oetomo Carolina Damayanti Marpaung Ferry Sandra Halim , Johni Halim, Johni Hartono, Tiffany Hayuningtyas, Ria A. Hayuningtyas, Ria Aryani Himawan Halim Ichwan, Solachuddin Jauhari Arief Isya Hanin Juliawati, Mita Komariah Komariah Krisna, Yohanes Lee, Kyung H. Lee, Kyung Hoon Melanie Sadono Djamil, Melanie Sadono Melyda, Ananda Shelly Michelle, Michelle Moksidy, Jenyfer Ignatia N Santosa, Didi Ni Luh Putri Nugroho, Didi Pang, Tiffany Pratitis, Visi Endah Sadono, Melanie Scania, Alifah Evi Taramalinda, Elizabeth Yuliani Wita Anggraini