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All Journal e-GIGI Indonesian Journal of Cancer Chemoprevention IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) The Indonesian Biomedical Journal Narra J Indonesian Journal of Pharmaceutical Education Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu
Halim, Johni
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Uji Aktivitas Antioksidan Ekstrak Daun Amaranthus hybridus L. dalam Pelarut Heksana, Etil Asetat, dan Air Halim, Johni; Ranggaini, Monica D.; Tedjokusumo, Sabrina N.
e-GiGi Vol. 13 No. 1 (2025): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v13i1.56111

Abstract

Abstract: Noncommunicable diseases, such diabetes mellitus, atherosclerosis and cancer, caused by inter alia imbalance between free radicals and antioxidants within the body, known as oxidative stress. Antioxidants from outside the body are required as antioxidants within the body are not enough to ward off excessive free radicals. Amaranthus hybridus L. plant is quite popular among Indonesian people due to its fast harvest and advantages for health, including its natural antioxidants potency. This study aimed to determine the comparison of antioxidant activities among n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. leaves. This was an experimental laboratory study. Amaranthus hybridus L. simplicia extracted with maceration in n-hexane, ethyl acetate and aquadest. Antioxidant activities of n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. were examined with 2.2-diphenyl-1-picrylhydrazyl (DPPH) in 517 nm wavelength by using microplate reader. The results showed that the IC50 values of A. hybridus L. n-hexane, ethyl acetate, and aqueous extract respectively were 131.81 mg/mL, 103.53 mg/mL, and 79.61 mg/mL. In conclusion, there are differences in antioxidant activities between n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. This occur due to differences in the polarity of each solvent. Keywords: Amaranthus hybridus L.; antioxidant activities; maceration; solvent polarity    Abstrak: Penyakit tidak menular, seperti diabetes melitus, aterosklerosis, dan kanker, disebabkan antara lain oleh ketidakseimbangan antara radikal bebas dan antioksidan dalam tubuh (stres oksidatif). Antioksidan dari luar tubuh diperlukan karena antioksidan dari dalam tubuh tidak cukup untuk menangkal radikal bebas berlebih. Tanaman Amaranthus hybridus L. cukup diminati masyarakat Indonesia karena waktu panen yang singkat dan manfaatnya bagi kesehatan, termasuk potensi antioksidan alami. Penelitian ini bertujuan untuk mengetahui perbedaan aktivitas antioksidan ekstrak heksana, etil asetat, dan air daun A. hybridus L. Jenis penelitian ini berupa studi eksperimental laboratorik. Simplisia A. hybridus L. diekstraksi dengan metode maserasi dalam pelarut heksana, etil asetat, dan air. Aktivitas antioksidan ekstrak heksana, etil asetat, dan air daun A. hybridus L. diuji aktivitas antioksidan dengan metode 2,2-difenil-1-pikrilhidrazil (DPPH) pada panjang gelombang 517 nm dengan menggunakan microplate reader. Hasil penelitian menunjukkan nilai IC50 ekstrak heksana, etil asetat, dan air daun A. hybridus L. secara berturut-turut sebesar 131,81 mg/mL, 103,53 mg/mL, dan 79,61 mg/mL. Simpulan penelitian ini ialah terdapat perbedaan aktivitas antioksidan antara ekstrak heksana, etil asetat, dan air daun Amaranthus hybridus L. Hal ini terjadi karena perbedaan polaritas dari masing-masing pelarut. Kata kunci: Amaranthus hybridus L.; aktivitas antioksidan; maserasi; polaritas pelarut
Pelatihan Peningkatan PHBS dan Pengelolaan Air Siap Konsumsi pada Masyarakat Wilayah RW 006, Kelurahan Tanjung Duren Selatan Halim, Johni; Louisa, Marie; Ariesanti, Yessy; Ayu Ratih Utari Mayun, I Gusti; Widhianingsih, Dhyani; Denilson
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 1 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i1.20119

Abstract

Water is crucial for human survival, serving both consumption needs and daily activities. However, water contamination often occurs when it is not managed and stored properly. Contaminated drinking water can pose health risks, causing various digestive diseases and even death, especially in children. Therefore, the management and storage of water require special attention. Indonesian communities, especially those in the RW 006, Tanjung Duren Selatan, are still limited in gathering clean water without proper water management for it to be made suitable for consumption. In response to this concern, a program was implemented to enhance awareness and empower the residents of RW 006, Tanjung Duren Selatan, focusing on improving environmental sanitation and the effective management of safe drinking water. The activities took place at the secretariat office of RW 006, Tanjung Duren Selatan, on Friday, November 10, 2023, from 08:30 to 10:15 AM. A total of 25 participants filled out attendance and completed pre-test questionnaires. Participants were then provided with education and training by the team on environmental sanitation, Community-Based Total Sanitation (STMB), proper management of household waste, and the handling of water intended for consumption. The post-test results showed an improvement in participants' knowledge and understanding regarding the enhancement of environmental sanitation and the management of water ready for consumption in households. It is hoped that participants will serve as links in communication and educators within the local community, ultimately contributing to improved sanitation and an enhanced quality of life.
Penyuluhan Kesehatan Gigi dan Pelatihan Penanganan Bau Mulut pada Masyarakat Kelurahan Jatipulo Jakarta Barat Soesanto, Sheila; Pratama Sarwono, Aditya; Halim, Johni; Eddy; Yuliasari, Hani
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 1 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i1.20143

Abstract

Bad breath, or commonly known as halitosis, is a health problem that is often overlooked. It can harm a person's daily life, including social isolation, anxiety, and discomfort. Halitosis can be divided into two types: genuine halitosis (related to oral issues) and delusional halitosis (related to false perceptions). Most cases of halitosis are caused by oral hygiene problems such as reduced saliva, dry mouth, gingivitis, periodontitis, and the use of dentures. Specific bacteria in the mouth can produce foul-smelling compounds known as volatile sulfur compounds (VSCs), such as methyl mercaptan and hydrogen sulfide. Additionally, halitosis can also result from systemic issues or bad habits. It is important to provide education to the community on how to address halitosis and maintain oral hygiene. Steps like regular tooth brushing, tongue cleaning, using dental floss, and avoiding strongly aromatic foods can help reduce the risk of halitosis. In the community of RT015/RW008, Kelurahan Jatipulo, West Jakarta, this education is particularly crucial because the level of knowledge about oral health is low. Through education and training, it is hoped that the community can recognize the causes of halitosis and address the issue before seeking medical help. This will improve the quality of life for the community, boost their self-confidence, and help prevent more serious oral health.
Aktivitas Antioksidan dengan Metode DPPH dan ABTS Terhadap Ekstrak Etanol Daun Amaranthus hybridus L. Dewi Ranggaini, Monica; Halim, Johni; Aurelia Tjoe, Michelle
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20891

Abstract

Background: Cancer, a degenerative disease, arises from free radicals in the body, which can be counteracted by antioxidants. Amaranthus hybridus L., a vegetable rich in antioxidant compounds like flavonoids, requires extraction to harness these compounds. This extraction uses the maceration technique with 70% ethanol. Antioxidant activity is tested using methods like DPPH and ABTS assays, with the IC50 value indicating effectiveness. Purpose: To determine the difference in antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods. Method: Laboratory experimental research. Simplicia leaves of A. hybridus L. in 70% ethanol solvent were extracted using maseration method. The antioxidant activity was tested in the sample and the ascorbic acid using DPPH and ABTS methods at wavelenghts 517 nm and 745 nm. Sample absorbance was measured using microplate reader. Result: IC50 values of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods were 54,99 µg/mL and 71,45 µg/mL, for ascorbic acid using DPPH and ABTS methods were 0,62 µg/mL and 3.13 µg/mL. Conclusion: The antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH method has stronger antioxidant than ABTS method.
Apium graveolens leaf ethanolic extract triggers apoptosis in human tongue cancer cells via caspase-3 and poly(ADP-ribose) polymerase pathways: An in vitro study Sandra, Ferry; Hartono, Tiffany; Hayuningtyas, Ria A.; Ranggaini, Dewi; Halim, Johni; Lee, Kyung H.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1634

Abstract

Recent advances in cancer treatment have focused on developing alternative therapies with reduced adverse effects. Chemoprevention using natural products derived from plants has gained significant attention. Apium graveolens has demonstrated anticancer properties against various cancer cell types, suggesting its potential efficacy against tongue cancer cells. The aim of this study was to evaluate the cytotoxic effects and mechanisms of action of Apium graveolens leaf ethanolic extract (AGLEE) on the HSC-3 tongue cancer cell line. The leaves were processed and extracted with 70% ethanol to obtain an ethanolic extract. HSC-3 cells were cultured, subjected to starvation, and pre-treated with or without Z-DEVD-FMK, a caspase-3 inhibitor. Subsequently, the cells were treated with or without doxorubicin or varying concentrations of AGLEE. To assess cell viability and apoptosis, MTT and sub-G1 assays were performed. Additionally, treated HSC-3 cells were collected, lysed, and analyzed for levels of cleaved-caspase-3 and cleaved-poly (ADP-ribose) polymerase (cleaved-PARP) using ELISA. The inhibitory concentration (IC50) value of AGLEE for reducing viable HSC-3 cells was determined to be 48.29 μg/mL. AGLEE significantly decreased HSC-3 cell viability and increased the percentage of apoptotic cells. It exhibited a concentration-dependent reduction in cell viability and an increase in apoptosis. Furthermore, the extract elevated the levels of cleaved-caspase-3 and cleaved-PARP in HSC-3 cells. Pre-treatment with Z-DEVD-FMK reduced the levels of cleaved-caspase-3 and cleaved-PARP induced by AGLEE. Taken together, AGLEE could be proposed as a potential natural therapeutic agent by inducing apoptosis through the caspase-3/PARP pathway in tongue cancer cells.
Antibacterial Efficacy of AgNO₃ Combined with Cymbopogon citratus Extract and Chitosan Nanocomposite Against Pseudomonas aeruginosa Azrianty, Annisa; Komariah, Komariah; Ranggaini, Monica Dewi; Anggraeni, Rezky; Halim, Johni; Nugroho, Didi
Indonesian Journal of Pharmaceutical Education Vol 5, No 2 (2025): May–August 2025
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v5i2.30641

Abstract

Infections caused by pathogenic bacteria such as Pseudomonas aeruginosa present significant challenges in clinical practice, particularly due to rising resistance to conventional antibiotics. The development of environmentally friendly, nanotechnology-based antibacterial agents is considered a promising alternative. This study aimed to evaluate the antibacterial activity of a nanocomposite comprising silver nitrate (AgNO₃), Cymbopogon citratus leaf extract, and chitosan against P. aeruginosa. The nanocomposite was synthesized using a green synthesis method, with plant extract serving as a natural reducing agent and chitosan as a nanoparticle stabilizer. Antibacterial activity was assessed via disk diffusion against five concentrations (6.25, 12.5, 15, 25, and 50 mg/mL), and compared to positive (chlorhexidine) and negative (acetic acid) controls. The results showed that the 6.25 mg/mL concentration produced the largest inhibition zone (average 11 mm), although it did not surpass the effectiveness of chlorhexidine. The inhibition zones remained stable for up to 72 hours, indicating sustained antibacterial activity. A decline in efficacy at 50 mg/mL was observed, likely due to nanoparticle aggregation and biological saturation. These findings support the potential of AgNO₃–C. citratus–chitosan nanocomposite as a natural-based alternative antibacterial agent. Further studies are recommended to characterize its physicochemical properties, elucidate its mechanism of action, and evaluate its toxicity and applicability in pharmaceutical and biomedical contexts.
Inositol Hexakisphosphate (InsP₆) Induces Apoptosis via Caspase-Dependent Pathways: Molecular Docking Insights Sandra, Ferry; Ranggaini, Dewi; Halim, Johni; Pakpahan, Alfred; Pratitis, Visi Endah; Lee, Kyung Hoon
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3810

Abstract

BACKGROUND: Inositol hexakisphosphate (InsP₆) exhibits anticancer activity, especially by inducing intrinsic and extrinsic apoptotic pathways. However, there is still no molecular docking evidence that directly examines InsP₆ interactions with either upstream or downstream apoptotic regulators. Therefore, the current study was conducted to investigate the molecular docking of InsP₆ to caspases as upstream/downstream apoptotic regulators.METHODS: Ligands including InsP₆, InsP₅, InsP₄, histone deacetylase inhibitor, and caspase inhibitors were retrieved from PubChem, while target proteins (histone, caspase-8, caspase-2, and caspase-3) were obtained from the Protein Data Bank. Ligand toxicity was predicted using ProTox-3.0, and physicochemical properties were analyzed with SwissADME. Ligand structures were energy-minimized using PyRx with the Universal Force Field, while proteins were prepared by removing water molecules and non-essential heteroatoms in BIOVIA Discovery Studio. Molecular docking was conducted using CB-Dock 2.0, with binding poses selected based on the lowest Vina score, and ligand–protein interactions were visualized in Discovery Studio.RESULTS: Molecular docking results showed that InsP₆ bound strongly to histone, caspase-8, caspase-2, and caspase-3 with affinities comparable to reference inhibitors, forming multiple hydrogen bonds with key active-site residues. InsP₆, InsP₅, and InsP₄ exhibited several similar binding sites to caspase-3, with only minor differences in binding affinity.CONCLUSION: InsP₆ shows strong binding to histone, caspase-8, caspase-2, and caspase-3 based on in silico results, supporting its role in inducing both extrinsic and intrinsic apoptotic pathways. Taken together, InsP₆ could be a potential inducer of apoptosis in cancer cells.KEYWORDS: cancer, apoptosis, InsP₆, InsP₅, InsP₄, caspase, in silico, molecular docking
Stenochlaena palustris Ethanol Extract Decreases Viability and Induces G1-Phase Cell Cycle Arrest in HSC-3 Tongue Cancer Cells via p21 and p27 Sandra, Ferry; Ranggaini, Dewi; Halim, Johni; Taramalinda, Elizabeth Yuliani; Scania, Alifah Evi; Roeslan, Boedi Oetomo; Lee, Kyung Hoon
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3308

Abstract

BACKGROUND: Oral squamous cell carcinoma (OSCC) of the tongue is an aggressive cancer with a poor prognosis due to its resistance to standard treatments. Stenochlaena palustris, a medicinal fern containing bioactive compounds, has shown potential anticancer properties. However, there is a lack of studies addressing the effects of S. palustris ethanol extract (SPEE) on tongue cancer. This study examined the effects of SPEE on the cell viability and cell cycle of human squamous cell carcinoma (HSC)-3 tongue cancer cells.METHODS: SPEE was prepared with the maceration method. HSC-3 cells were treated with SPEE at concentrations of 100, 500, and 1000 µg/mL for 24 and 48 hours. Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis was performed using flow cytometer. Immunoblotting was used to measure amount of cell cycle regulators, protein 21 (p21) and protein 27 (p27).RESULTS: SPEE treatment led to a significant decrease in HSC-3 viable cells in a concentration- and time-dependent manner, with the most pronounced effect at higher concentration and prolonged treatment time. There was a slightly increase in the percentage of cells in the Sub-G1 phase in SPEE-treated group, meanwhile there was a significant increase in the percentage of cells in the G1-phase. Increased amount of p21 and p27 were observed in SPEE-treated group.CONCLUSION: SPEE significantly inhibited HSC-3 cell proliferation in a concentration- and time-dependent manner, primarily by inducing G1-phase cell cycle arrest through the upregulation of p21 and p27. Taken together, SPEE could be a potential anti-cancer agent for tongue cancer cell. KEYWORDS: Stenochlaena palustris, tongue cancer, cytotoxic, cell cycle arrest, HSC-3 cells, p21, p27
Curcuma xanthorrhiza Rhizome Extract Induces Apoptosis in HONE-1 Nasopharyngeal Cancer Cells Through Bid Ranggaini, Dewi; Sandra, Ferry; Halim, Johni; Ichwan, Solachuddin Jauhari Arief; Djamil, Melanie Sadono
The Indonesian Biomedical Journal Vol 15, No 1 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i1.2217

Abstract

BACKGROUND: Curcuma xanthorrhiza rhizomes have been demonstrated to have anticancer properties toward various types of cancer cells. The effect of C. xanthorrhiza rhizome extract (CXRE) on nasopharyngeal cancer (NPC) cells, including HONE-1 cell line has not been elucidated yet. Therefore, the effect of CXRE on the apoptosis of HONE-1 cells and its possible underlying mechanism are necessary to be explored.METHODS: C. xanthorrhiza rhizomes were minced, dried, extracted with distilled ethanol, filtered, and evaporated to produce CXRE. HONE-1 cells were seeded, starved, and treated with dimethyl sulfoxide (DMSO), Doxorubicin, or various concentrations of CXRE. Treated HONE-1 cells were stained with 4',6'-diamidino-2-phenylindole (DAPI) and the number of viable cells was counted. HONE-1 cells were also collected, lysed, and further processed for immunoblotting analysis to measure Bid activity.RESULTS: The number of viable HONE-1 cells decreased in concentration- and time-dependent manner. The number of viable cells in 50 and 250 μg/mL CXRE-treated groups were significantly lower compared with that in the DMSO-treated group after 24 h. At 48 h incubation period, the number of viable cells in 10, 50 and 250 μg/mL CXRE-treated groups were significantly lower compared with that in the DMSO-treated group. The number of viable cells in 250 μg/mL CXRE-treatment group was not significantly different compared with that in the Doxorubicin-treated group after 48 h. Bid expression levels in CXRE-treated groups were lower compared with that in the DMSO-treated group.CONCLUSION: CXRE could induce apoptosis via Bid activation, hence reducing the viability of HONE-1 cells.KEYWORDS: Curcuma xanthorrhiza, nasopharyngeal cancer, HONE-1 cells, apoptosis, Bid
Bone Resorption in Ameloblastoma and Its Underlying Mechanism Jackson, Jackson; Halim, Johni; Anggraeni, Rezky; Sandra, Ferry
Indonesian Journal of Cancer Chemoprevention Vol 12, No 1 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss1pp57-66

Abstract

Ameloblastoma, a tumor located in the jaw, grows slowly but locally invasive. Ameloblastoma expands in the jaw based on a mechanism resorbing the surrounding bone. To date, the bone resorption mechanisms of ameloblastoma are associated with the expression of receptor activator of nuclear factor (NF)-κB (RANK) ligand (RANKL), matrix metalloproteinases (MMPs), and tumor necrosis factor (TNF)-α. RANKL plays an important role in generating osteoclastogenesis. MMPs degrade the extracellular matrix. TNF-α can induce the formation of osteoclast and modulate the MMPs. In this review the bone resorption mechanism of ameloblastoma as well its signaling pathway will be disclosed.Keywords: Ameloblastoma, RANKL, MMPs, TNF-α.
Co-Authors Alfred Pakpahan, Alfred Anggraeni, Rezky Ariesanti, Yessy Aurelia Tjoe, Michelle Azrianty, Annisa Boedi Oetomo Roeslan, Boedi Oetomo Denilson Dewi Ranggaini Dhyani Widhianingsih Eddy Eddy Ferry Sandra Hartono, Tiffany Hayuningtyas, Ria A. I Gusti Ayu Ratih Utari Mayun Ichwan, Solachuddin Jauhari Arief Jackson Jackson, Jackson Komariah Komariah Lee, Kyung H. Lee, Kyung Hoon Louisa, Marie Melanie Sadono Djamil, Melanie Sadono Melyda, Ananda Shelly Nugroho, Didi Pratama Sarwono, Aditya Pratitis, Visi Endah Ranggaini, Dewi Ranggaini, Monica D. Ranggaini, Monica Dewi Scania, Alifah Evi Soesanto, Sheila Taramalinda, Elizabeth Yuliani Tedjokusumo, Sabrina N. Yuliasari, Hani