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All Journal Jurnal Natur Indonesia Jurnal Natur Indonesia
Mohammad Candra Prayoga
Department of Agronomy, Faculty of Agriculture, University of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Physics

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Optimization of TDZ Hormone on the Formation of Somatic Embryogenesis in Dendrobium Orchids (D.50TH Stage Beauty X D. Bobby Mesina) Didik Pudji Restanto; Iqbal Felayati; Wahyu Indra Duwi Fanata; Parawita Dewanti; Budi Kriswanto; Mohammad Nur Khozin; Mohammad Candra Prayoga
Jurnal Natur Indonesia Vol 21, No 1 (2023): April 2023
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.1.42-46

Abstract

The Dendrobium variety orchid is a plant that has rare potential and growth. This requires large amounts of seeds that can be propagated in vitro. This study aims to determine the effect of TDZ on the direct induction of somatic embryogenesis. The study was conducted using a completely randomized design (CRD) with one factor, namely media consisting of basal Murashige and Skoog (MS) media with growth regulator TDZ. Each treatment out of a total of seven treatments was repeated four times. The research was carried out from March 2022 to August 2022. The results showed that leaf explants (clonal) could only be induced in 2 treatments (1 mg/L and 1.5 mg/L) out of 28 experimental media treatment units. The earliest callus appeared in the 1 mg/L treatment with a growth time of 90 days after planting (DAP) and the earliest PLB appeared in the 1.5 mg/L treatment with a growing time of 45 days after planting (DAP), the percentage of explant growth that became PLB with 1.5 mg/L treatment resulted in a percentage of 20 % and the percentage of callus growth at 1 mg/L yields a percentage of 20%.
The Effect of Benzyl Amino Purine (BAP) and Coconut Water on the Growth of Vanilla (Vanilla planifolia Andrews.) In Vitro Dwi Erwin Kusbianto; Nurhayadatul Haliza; Didik Pudji Restanto; Distiana Wulanjari; Sholeh Avivi; Mohammad Candra Prayoga
Jurnal Natur Indonesia Vol. 22 No. 2 (2024): October
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.22.2.76-83

Abstract

Vanilla (Vanilla planifolia Andrews.) is one of the plantation crops that has a high selling price, namely wet vanilla IDR 200,000-300,000/kg, regular quality dry vanilla around 1-3 million/kg, and export quality reaching 5-7 million/kg. The opportunity for farmers to develop vanilla commodities in Indonesia is very large, but limited planting materials still hamper it. In-vitro propagation is one technique that can be used to overcome the problem of vanilla propagation in Indonesia. This research aimed to determine the interaction of BAP and coconut water on the growth of vanilla nodal explants in vitro. The method used in this research was a factorial Completely Randomized Design (CRD) with a combination of Benzyl Amino Purine (BAP) and coconut water. The BAP concentration consists of 0 mg/L, 1 mg/L, and 2 mg/L. Coconut water has 0%, 15%, and 30% levels. Data were analyzed using ANOVA and further tested with the DMRT test at a confidence level of 95%. The results showed an interaction between BAP and coconut water on the growth of vanilla explants. The combination treatment of 1 mg/L BAP + 15% coconut water showed the best results with the fastest response time of 8.76+1.53 DAP, average shoot length 2.84±0.70 cm/explant and average root length 0.75±0.07 cm/explant
Optimization of TDZ Hormone on the Formation of Somatic Embryogenesis in Dendrobium Orchids (D.50TH Stage Beauty X D. Bobby Mesina) Didik Pudji Restanto; Iqbal Felayati; Wahyu Indra Duwi Fanata; Parawita Dewanti; Budi Kriswanto; Mohammad Nur Khozin; Mohammad Candra Prayoga
Jurnal Natur Indonesia Vol. 21 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.1.42-46

Abstract

The Dendrobium variety orchid is a plant that has rare potential and growth. This requires large amounts of seeds that can be propagated in vitro. This study aims to determine the effect of TDZ on the direct induction of somatic embryogenesis. The study was conducted using a completely randomized design (CRD) with one factor, namely media consisting of basal Murashige and Skoog (MS) media with growth regulator TDZ. Each treatment out of a total of seven treatments was repeated four times. The research was carried out from March 2022 to August 2022. The results showed that leaf explants (clonal) could only be induced in 2 treatments (1 mg/L and 1.5 mg/L) out of 28 experimental media treatment units. The earliest callus appeared in the 1 mg/L treatment with a growth time of 90 days after planting (DAP) and the earliest PLB appeared in the 1.5 mg/L treatment with a growing time of 45 days after planting (DAP), the percentage of explant growth that became PLB with 1.5 mg/L treatment resulted in a percentage of 20 % and the percentage of callus growth at 1 mg/L yields a percentage of 20%.
Co-Authors Budi Kriswanto Didik Pudji Restanto Didik Pudji Restanto Distiana Wulanjari Dwi Erwin Kusbianto Iqbal Felayati Iqbal Felayati Mohammad Nur Khozin Nurhayadatul Haliza Parawita Dewanti Parawita Dewanti Sholeh Avivi Wahyu Indra Duwi Fanata Wahyu Indra Duwi Fanata