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All Journal Jurnal Biologi Tropis Journal of Science and Science Education (JoSSEd)
Ernin Hidayati
University of Mataram
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Immunology & microbiology Materials Science & Nanotechnology Neuroscience Physics

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Growth and Antibiotic Sensitivity Status of Bacillus spp. associated with Abalone (Haliotis asinina) as Probiotic Candidate Selection Faturrahman Faturrahman; Nurul Zulfa Fitriana; Sarkono Sarkono; Ernin Hidayati
Journal of Science and Science Education Vol. 4 No. 1 (2023): April
Publisher : Pascasarjana, Mataram University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jossed.v4i1.3089

Abstract

The use of probiotics is an innovation in an effort to increase production and fight pathogens in aquaculture cultivation environments. Probiotic candidate microorganisms are selected selectively to ensure that the probiotics used are safe and provide benefits for the host and the environment. This study aims to select probiotic candidates based on the aspects of growth and sensitivity to antibiotics from 4 isolates of Bacillus from abalone (Haliotis asinina). Growth observations were carried out by turbidimetric method using a spectrophotometer at a wavelength of 600 nm in SWC media, and antibiotic sensitivity was tested using Rifampicin, Gentamicin, Kanamycin, and Erythromycin antibiotics using the well-diffusion agar method. The growth analysis of isolates was described by a growth curve based on the OD value, and antibiotic susceptibility based on the diameter of the inhibition zone. The results showed that the growth of the four isolates of Bacillus spp. had a short lag phase, ranging from 1-2 hours, an exponential phase ranging from 15 hours to B. pumilus SLK1 and B. licheniformis SLK2 isolates, and 12 hours to B. coagulans CaK1 and B. coagulans CaK6 isolates. Antibiotic sensitivity showed that B. coagulans CaK1 isolates were sensitive to all the tested antibiotics, while B. pumilus SLK1 isolates, B. licheniformis SLK2, and B. coagulans CaK6 were only sensitive to Rifampicin and Gentamicin antibiotics. The ideal probiotic candidate is an isolate with good growth and does not have resistance properties, based on the results of this study CaK1 isolate has the potential as a probiotic candidate
Isolation of Phosphate Solubilizing Bacteria Around Bamboo (Bambusa vulgaris) Clumps Using Natural Media Trap Method Parisya Velayati Furqon; Bambang Fajar Suryadi; Ernin Hidayati
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5074

Abstract

Bamboo plant is a potential source of plant growth promoting bacteria (PGPB). Phosphate solubilizing ability is an important characteristic of PGPB. This study aims to isolate phosphate solubilizing bacteria around the bamboo clumps using natural media. The bacteria around the bamboo clumps are trapped using natural media. The media consist of  70 gram cooked rice and 70 gram banana peel. The media were stored in plastic boxes with four types of treatments: 1) tightly closed, 2) covered with tissue, 3) covered with banana leaves, and 4) uncovered (open). Those plastic boxes are placed around the bamboo clump and left  for up to 5 days. The trapped phosphate solubilizing bacteria were isolated using Pikovskaya agar. A total of 17 isolates of phosphate solubilizing bacteria were successfully trapped, 8 isolates in cooked rice and 9 isolates in banana peel. The isolates showed a phosphate solubilization index, ranging from 0,2 to 1,40. The isolate NN01 has the highest ability to solubilize phosphate. This isolate was trapped in a cooked rice with an open plastic box condition. NN01 is a Gram positive rod-shaped bacterium. In this study it was revealed that the area of ​​the bamboo clumps is inhabited by various types of bacteria. The natural media of cooked rice and banana peels have a good ability to trap potential phosphate solubilizing bacteria around the bamboo clumps.
Co-Authors Faturrahman Nurul Zulfa Fitriana Parisya Velayati Furqon Sarkono Sarkono Suryadi, Bambang Fajar