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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Transformasi: Jurnal Pengabdian Masyarakat Jurnal Farmasi Sains dan Komunitas Jurnal Sains Teknologi dan Lingkungan (JSTL) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Ilmu Kefarmasian Indonesia Jurnal Abdi Insani Bioscientist : Jurnal Ilmiah Biologi Jurnal Ilmiah Sangkareang Mataram Journal of Science and Science Education (JoSSEd) Jurnal Warta Desa (JWD) Jurnal Pendidikan dan Pengabdian Masyarakat Samota Journal of Biological Sciences (SJBIOS)

Suryadi, Bambang Fajar

PS BIOLOGI, FAKULTAS MIPA UNIVERSITAS MATARAM

Author-ID : 1252310

Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Aktivitas Antibakteri Bacillus yang Berasosiasi dengan Landak Laut di Pantai Mentigi, Lombok Barat Suryadi, Bambang Fajar; Febrian, Novi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The search for new antimicrobial agents is very significant. The prevalence of antimicrobialresistance among key microbial pathogens is increasing at an alarming rate worldwide. Bacillusspecies produce many kinds of antibiotics which share a full range of antimicrobial activities.The aim of this research is to study antibacterial activity of three bacillus isolates (1A, 2J, 3L)which were isolated in Mentigi Beach, West Lombok. Assessment for antibacterial activity wasconducted using Overlaid Molten agar method on Nutrient Agar medium at different salinity(concentration of NaCl 0, 5, 10%) and different pH (pH 6, 7 and 8). The study showed that therewere different antibacterial activities at different salinity and different pH media. Production ofantibacterial at 0% NaCl concentration occurred only at pH 6 and 7 and had narrow spectrumcharacter (only for Gram positive bacteria). Addition of 5% NaCl made antibacterialproduction might occur at pH 6, 7 and 8 and had wide spectrum character (for Gram positiveand negative bacteria).

The Use of Fish Flour and Palm Sugar Medium to Grow Mataram Indigenous Isolate of Entomopathogenic Bacillus thuringiensis for Controlling Aedes agypti Larvae Suryadi, Bambang Fajar; Hidayat, Hilal Aisyah; Tresnani, Galuh
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

To grow Mataram indigenous local isolate of entomopathonic Bacillus thuringiensis using 4 combinations of fish flour and palm sugar natural liquid medium. The culture was then tested against 3rd instar Aedes aegypti larvae to gain lethal concentration (LC) value within 72-hour test. Four combinations of fish flour and palm sugar liquid medium (in 1:1; 1:3; 1:5 and 1:7 weight ratio) were made to grow B. thuringiensis isolate Bt-TP2B. Cell, endospore and protein crystal were recorded every 24 hours within 72-hour incubation. Seventy-two-hour-old B. thuringiensis cultures from those media were serially diluted, then tested against 3rd instar B. thuringiensis larvae. Larval death was recorded every 24 hours within 72-hour observation to obtain lethal concentration (LC) values. Results from all medium were compared to those of NYSM standar medium. B. thuringiensis isolate BT-TP2B could grow in fish flour and palm sugar natural medium. The highest toxicity (the lowest LC) was seen on B. thuringiensis grown on fish flour and palm sugar natural medium in 1:1 weight ratio. The LC50 values were 1.98x103 cell/mL (in 24-hour observation) and 1.49x103 cell/mL (in 48-hour and 72-hour observation), while LC90 values were 1.35x103 cell/mL in 24-hour observation and 1.58x103 cell/mL in 48 and 72-hour observations). These values were lower (more toxic) compared to those of NYSM standard medium. Fish flour and palm sugar natural medium could be used to grow entomopathogenic B. thuringiensis. Moreover, this natural medium could increase toxicity of B. thuringiensis against B. thuringiensis3rd instar larvae.Â

Perbandingan Aktivitas Antimikroba Ekstrak Etanol dari Tiga Spesies Ganoderma Asal Pulau Lombok: Comparison of Antimicrobial Activities of Ethanol Extract from Three Species of Ganoderma Original Lombok Island Faturrahman Faturrahman; Sukiman Sukiman; Bambang Fajar Suryadi; Sarkono Sarkono; Ernin Hidayati
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 7 No. 2 (2021): JURNAL SAINS TEKNOLOGI & LINGKUNGAN
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v7i2.282

The use of antibiotics is one of the most important ways to deal with the spread and treatment of pathogenic microbial infections. The search for new antibiotic sources continues to be carried out to anticipate the emergence of microbial resistance. One of the natural resources that has the potential as an antimicrobial source is a member of the macrofungi of the Genus Ganoderma. The purpose of this study was to evaluate the antimicrobial performance of the ethanol extracts of Ganoderma lucidum, G. applanatum dan Ganorderma sp. against fungi (Candida albicans dan Cryptococcus neoformans), gram positive bacteria (Bacillus cereus dan Staphylococcus aureus) and gram negative bacteria (Escherichia coli dan Shigella sp.). Macrofungi samples were taken from the forest area of Nature Tourism Park (TWA) Gunung Tunak, TWA Kerandangan, TWA Suranadi, TWA Nuraksa Sesaot, TWA Lemor and Pusuk forest. The stages of the method performed are sample collection, sample preparation, extraction, and testing of antimicrobial activity using the well difusion method. The ethanol extract concentrations for testing were 20%, 40%, 60% and 80%. The results showed that the three Ganoderma species had anti-fungal and antibacterial activity and that different levels of concentration had an effect on inhibition. The size of the inhibition zone is directly proportional to the higher the extract concentration. The antimicrobial activity of the ethanol extract of G. lucidum was higher when compared to G. applanatum and Ganoderma sp. both against fungi (Candida albicans and Cryptococcus neoformans) as well as against gram-positive and gram-negative test bacteria. In addition, G. applanatum showed very weak inhibition against both groups of tested bacteria. Key words: candidiasis; cryptococcosis; comorbid infections; macerations; pathogens

Aktivitas Antibakteri Bacillus yang Berasosiasi dengan Landak Laut di Pantai Mentigi, Lombok Barat Bambang Fajar Suryadi; Novi Febrian
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2586

Penyuluhan upaya penanggulangan dan pemeriksaan cacingan sebagai implementasi program pesantren sehat Atwazzah Taisir; Ria Hapsari; Iman Surya Pratama; Siti Rahmatul Aini; Galuh Tresnani; Bambang Fajar Suryadi
Transformasi: Jurnal Pengabdian Masyarakat Vol. 15 No. 2 (2019): Transformasi Desember
Publisher : LP2M Universitas Islam Negeri Mataram

[Bahasa]: Angka prevalensi cacingan nasional pada tahun 2015 sebesar 28,12%, dengan prevalensi daerah yang bervariasi hingga melebihi 50%. Survey cacingan pada sampel anak SD/MI di Kabupaten Lombok Barat pada tahun 2011 menunjukkan angka prevalensi 29,47%. Cakupan upaya penanggulangan yang masih terbatas mendorong peningkatan koordinasi lintas mitra salah satunya pesantren. Program pengabdian kepada masyarakat ini bertujuan untuk mengimplementasikan upaya penanggulangan cacingan melalui penyuluhan dan demonstrasi indikator Perilaku Hidup Bersih dan Sehat (PHBS) pada salah satu mitra pondok pesantren di daerah Lombok Barat. Metode pengumpulan data yang digunakan yaitu wawancara semi terstruktur dan observasi pengetahuan siswa dengan lembar kuisioner. Berdasarkan pemeriksaan natif terhadap 38 sampel feses terdapat 3 orang cacingan (0.7 %) terdiri atas infeksi campuran cacing tularan tanah (Ascaris sp dan Trichuris sp) dan infeksi tunggal (Hymenolepis sp, Ascaris sp). Hasil pengabdian secara umum berkontribusi terhadap penguasaan indikator PHBS sederhana yang terdiri atas: Cuci Tangan Pakai Sabun (CTPS), penggunaan alas kaki, serta pengukuran berat, dan tinggi badan secara berkala. Kata Kunci: cacingan; pesantren sehat; PHBS [English]: The national worm prevalence rate in 2015 was 28.12%, with regional prevalence varies over 50%. Worms survey in a sample of elementary school children in West Lombok Regency in 2011 showed a prevalence rate of 29.47%. The limited scope of prevention efforts has led to increased coordination across partners including pesantren. This community service program aimed to implement the prevention of intestinal worms through counseling and demonstration of clean and healthy behaviors (PHBS) indicators at one of the boarding schools in West Lombok. The methods used in this program is semi-structured interview and observation of students’ knowledge with questionnaire sheets. Based on the native examination of 38 feces samples, there were three students who have worms (0.7%) consisting of mixed infection of earthworm (Ascaris sp and Trichuris sp) and single infection (Hymenolepis sp, Ascaris sp). Generally, this program contributes to the mastery of simple PHBS indicators consisting of: handwashing with soap (CTPS), footwear use, and periodic weight and height measurements. Keywords: worm infestation; healthy boarding school; personal hygiene

ANTI-DIARRHEAL ACTIVITY OF AQUEOUS EXTRACT OF NAGASARI FLOWERS (Mesua ferrea L.) IN BALB/c MICE INDUCED BY Escherichia coli Putu Monik Ananta Puspitarini; Iman Surya Pratama; Bambang Fajar Suryadi
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 16, No 2 (2019)
Publisher : Sanata Dharma University

Nagasari (Mesua ferrea L.) is empirically used for curing diarrhea in Nusa Tenggara Barat Province. However, the safety and efficacy of nagasari have not been scientifically validated in animal model. This study evaluates the activity of nagasari flowers aqueous extract in BALB/c mice induced by Escherichia coli to determine the most effective dosage for anti-diarrheal effect. The aqueous extract of nagasari flowers ((0.4% (w/v), 0.8% (w/v), and 1.6% (w/v)) was given to 3 groups of mice whereas negative and positive control groups received aquadestilate and ciprofloxacin 130 mg/kgBW respectively orally once per day for 3 days. The frequency and consistency of feces were observed then the total colony of E. coli in feces was calculated using colony counter. Aqueous extract of nagasari flowers at concentration of 1.6% showed significant inhibitory activity against Escherichia coli compared to positive controls (diarrhea index of 0.425; p0.05 and total colony of E. coli in feces of 100.2; p0.05). This study provides scientific support for the traditional use of aqueous extract of nagasari flowers for the treatment of diarrheal diseases.

Efek Antidiare Ekstrak Air Bunga Nagasari (Mesua Ferrea L.) Terhadap Mencit BALB/c yang Diinduksi Minyak Jarak Putu Monik Ananta Puspitarini; Iman Surya Pratama; Bambang Fajar Suryadi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 1 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Nagasari (Mesua ferrea L.) has a empirically repute for management of diarrhea in West Nusa Tenggara Province. However, the safety and efficacy of the nagasari flowers extract have not been scientifically validated in animal model. This study wasaimed to investigate the antidiarrheal effect of water extract of the nagasari flowers in BALB/c mice. The antidiarrhea activity of nagasari flowerswas investigated using castor oil-induced diarrhea increase in diarrhea index in mice.In castor oil-induced diarrhea test, mice received various consentrations of nagasari flowers extract (0,4% w/v; 0,8% w/v; and 1,6% w/v), where as negative controls received CMC-Na 1% and positive controls received loperamide 0,7 mg/kgBW orally once daily for 1 day and the mice droppings were observed. The phytochemical screening of nagasari flowers contain flavonoid, saponin, and phenolic. Water extract of nagasari flowers at concentration 1,6% showed significant inhibitory activity against castor oil-induced diarrhea when compared with positive controls (diarrhea index 0,083 ; p>0,05). Therefore, this study provides a scientific support for the acclaimed traditional use water extract of Mesua ferrea L. for the treatment of diarrheal diseases.

UJI AKTIVITAS DAN PRODUKSI ANTIBAKTERI BACILLUS LENTUS YANG DIISOLASI DARI SISTEM PENCERNAAN LANDAK LAUT DALAM MENGHAMBAT BAKTERI PENYEBAB INFEKSI PADA KEHAMILAN I Gusti Agung Ayu Hari Triandini; Bambang Fajar Suryadi
JURNAL SANGKAREANG MATARAM Vol. 4 No. 2 (2018): Juni 2018
Publisher : SANGKAREANG

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penyakit infeksi pada kehamilan merupakan penyakit yang menempati peringkat ketiga penyebab kematian ibu dan anak setelah perdarahan dan hipertensi. Mikroorganisme dari genus Bacillus diketahui telah banyak menghasilkan jenis antibiotik. Diperlukan suatu penelitian lanjutan ke arah produksi antibakteri sekaligus optimalisasi fermentasi dalam media cair untuk memudahkan tahapan isolasi, Identifikasi, purifikasi dan karakterisasi senyawa antibakteri pada tahap selanjutnya. Faktor-Faktor yang dikaji dalam optimalisasi adalah: jenis medium, sumber nitrogen dan waktu inkubasi. Penelitian ini bersifat kuantitatif eksperimental. Variabel bebas: jenis medium, sumber nitrogen tambahan dan waktu inkubasi. Variabel terikat: diameter zona hambat, sedangkan variabel kontrol: diameter zona hambat Polymyxin B dan aquades steril. Jenis percobaan faktorial RAK yang terdiri dari 6 jenis bakteri uji sebagai blok. Faktor jenis medium: m1=NBS, m2=SAQ; faktor sumber N tambahan: n1=tanpa N, n2=NH4Cl, n3=asam glutamat; faktor waktu inkubasi t1=4 jam hingga t12=48 jam (kelipatan 4 jam). Tahapan kerja: aktivasi kultur stok, karakterisasi ulang kultur stok, penentuan waktu inkubasi kultur starter, pembuatan kultur starter, fermentasi, pengukuran pertumbuhan, pemanenan metabolit, pengujian antibakteri. Antibakteri Bacillus lentus sudah mulai diproduksi pada fase log. Produksi antibakteri Bacillus lentus di medium tanpa penambahan sumber nitrogen tinggi pada fase log (16-20 jam). Kombinasi medium SAQ tanpa penambahan sumber nitrogen memberikan rata-rata produksi antibakteri Bacillus lentus tertinggi. Antibakteri Bacillus lentus memiliki aktivitas penghambatan tertinggi pada Proteus mirabilis. Produksi antibakteri Bacillus lentus yang diproduksi pada medium SAQ tanpa penambahan sumber nitrogen ketika diujikan ke Proteus mirabilis pada waktu inkubasi 4 jam, 16 jam dan 20 jam dapat dipertimbangkan untuk dilanjutkan ke tahapan identifikasi, purifikasi dan karakterisasi antibakteri.

Effectiveness of Ethanol Extract among Three Ganoderma Species From Lombok in Inhibition Growth of Candida albicans and Cryptoccocus neoformans Fitria Rizka Hidayat; Sukiman Sukiman; Ernin Hidayati; Sarkono Sarkono; Bambang Fajar Suryadi; Faturrahman Faturrahman
Journal of Science and Science Education Vol. 1 No. 1 (2020): October
Publisher : Pascasarjana, Mataram University

Candida albicans and Cryptoccocus neoformans are opportunistic pathogenic fungi that cause infectious diseases that are the world's biggest health problems. The use of antibiotics is one way to overcome the spread of the infection and cause microbial resistance. Ganoderma is one of the many macrophages found on Lombok's island, and studies of its antifungal activity have not been carried out. The purpose of this study was to determine the antifungal potential and the effect of different concentrations of ethanolic extracts of three Ganoderma species on C. albicans and C. neoformans. Ganoderma samples were obtained from Suranadi Taman Wisata Alam (TWA), Sesaot TWA, Tunak Mountain TWA, Kerandangan TWA, and Pusuk Forest. Ganoderma extraction was carried out by the maceration method using ethanol 95% solvent. The extract concentrations used are 20%, 40%, 60% and 80%. This research was conducted using the wells method with metronidazole as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition zone formed around the well. The results obtained are the three species of Ganoderma have antifungal activity against test fungi, and different levels of concentration affect inhibition. The amount of the inhibition zone is directly proportional to the high concentration of the extract. All three Ganoderma species are more effective in inhibiting the growth of C. neoformans compared with Candida albicans

PEMBUATAN SPESIMEN AWETAN ORGANISME UNTUK MENUNJANG PELAJARAN KEANEKARAGAMAN HAYATI DI SEKOLAH Yuliadi Zamroni; Galuh Tresnani; Bambang Fajar Suryadi; Dining Aidil Candri; Sukiman Sukiman
Jurnal Warta Desa (JWD) Vol. 1 No. 2 (2019): Jurnal Warta Desa (JWD)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jwd.v1i2.33

Telah dilakukan pelatihan pembuatan spesimen awetan organisme kepada guru-guru di provinsi NTB. Kegiatan ini bertujuan untuk meningkatkan keterampilan guru dalam membuat media pembelajaran untuk menunjang pelajaran keanekaragaman hayati disekolah. Kegiatan ini dilakukan selama 4 hari dari tanggal 30 Oktober-2 November 2017 yang diikuti oleh 34 guru yang ada di NTB. Melalui kegiatan ini, para guru diperkenalkan tentang keanekaragaman hayati yang ada di NTB khususnya dan Indonesia pada umumnya. Selain itu para guru juga dilatih melakukan sampling dan pengawetan flora dan fauna yang ada disekitar lingkungan tempat tinggal. Tehnik fotografi dan manajemen spesimen awetan diberikan untuk menambah softskill para guru sehingga spesimen yang dikoleksi dapat disimpan dalam jangka waktu yang lama. Para peserta pelatihan memberikan respon yang positif terhadap kegiatan ini. Hal tersebut dapat diketahui melalui jumlah peserta yang melebihi target, antusiaisme dalam mengikuti setiap kegiaatan dan permintaan untuk diundang dalam pelatihan-pelatihan berikutnya yang diadakan Universitas Mataram.

Co-Authors Alfany, Chalida Annisa, Zuriatun Atwazzah Taisir Dewi, Novia Devi Kusuma Dining Aidil Candri, Dining Aidil Ernin Hidayati ERNIN HIDAYATI Ernin Hidayati Ernin Hidayati Ernin Hidayati Faturrahman Faturrahman Faturrahman Febrian, Novi Fitria Rizka Hidayat Galuh Tresnani Galuh Tresnani Hidayat, Hilal Aisyah I Gusti Agung Ayu Hari Triandini I WAYAN SUANA Ika Mustikasari, Ika Iman Surya Pratama Iman Surya Pratama Iman Surya Pratama ISLAMUL HADI Kurniasih Sukenti Kusuma, A. A. Ngurah Nara Kusuma, AA Ngurah Nara Kusuma, Anak Agung Nara Made Raningsih Novi Febrian Parisya Velayati Furqon Pita, Baiq Nuraini Ayu Prasedya, Eka Sunarwidhi Putri, Vita Septiana Sulaiman Putu Monik Ananta Puspitarini Putu Monik Ananta Puspitarini Ria Hapsari Sadewo, Imran Sarkono Sarkono Sidhiawan, Anak Agung Putu Siti Rahmatul Aini Sukiman Sukiman Sukiman Sukiman Sukiman Sukiman Sukiman Sukiman Taqwim, Ahsani Yuliadi Zamroni

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