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All Journal Torani Journal of Fisheries and Marine Science Marine Research in Indonesia
St. Hidayah Triana, St. Hidayah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Social Sciences

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THE EFFECT OF VITAMIN C AND AEROMONAS VACCINE ON THE IMMUNE RESPONSE AND DISEASE RESISTANCE OF GROUPER (Epinephelus fuscoguttatus) Ilmiah, -; Triana, St. Hidayah; Tassakka, Asmi Citra Malina A.R.; Rantetondok, Alex; Anshary, Hilal
Marine Research in Indonesia Vol 34, No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7346.903 KB) | DOI: 10.14203/mri.v34i2.512

Abstract

We evaluated the effectiveness of vitamin C and Aeromonas salmonicida vaccine in grouper (Epinephelus fuscoguttatus) for increasing immune responses and protection against A .salmonicida. The vitamin C used was polyethoxylated ascorbic and tocopherol. The vaccine was prepared from formalin-killed cells and concentrated extracellular products of a single isolate A. salmonicida. Bath immersion vitamin C and vaccine trials were conducted for 60 min. Fish used had a mean weight 25 g. Control groupers were injected with tryptic soy broth. The results showed that vitamin C enhanced phagocytic activity in head kidney leucocytes of grouper 7, 14, 28 and 36 days after treatments. A significant different of the antibody titre was found between control fish and the treated fish at 42 days after treatments. In addition, at day 42, Relative Percent Survival (RPS) for control group was 53.3 %, vitamin C-treated group was 80.0 % and vaccinated group was 90.0 %. The results of this study suggest that bath immersion of vitamin C provided an increasing of phagocytic activity (non-specific immune responses), titre antibody (specific immune responses) and protection against A. salmonicida infection in grouper. A. salmonicida vaccine also en-hanced titre antibody and protection against A. salmonicida infection in grouper.
THE EFFECT OF VITAMIN C AND AEROMONAS VACCINE ON THE IMMUNE RESPONSE AND DISEASE RESISTANCE OF GROUPER (Epinephelus fuscoguttatus) Ilmiah, -; Triana, St. Hidayah; Tassakka, Asmi Citra Malina A.R.; Rantetondok, Alex; Anshary, Hilal
Marine Research in Indonesia Vol 34 No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7346.903 KB) | DOI: 10.14203/mri.v34i2.512

Abstract

We evaluated the effectiveness of vitamin C and Aeromonas salmonicida vaccine in grouper (Epinephelus fuscoguttatus) for increasing immune responses and protection against A .salmonicida. The vitamin C used was polyethoxylated ascorbic and tocopherol. The vaccine was prepared from formalin-killed cells and concentrated extracellular products of a single isolate A. salmonicida. Bath immersion vitamin C and vaccine trials were conducted for 60 min. Fish used had a mean weight 25 g. Control groupers were injected with tryptic soy broth. The results showed that vitamin C enhanced phagocytic activity in head kidney leucocytes of grouper 7, 14, 28 and 36 days after treatments. A significant different of the antibody titre was found between control fish and the treated fish at 42 days after treatments. In addition, at day 42, Relative Percent Survival (RPS) for control group was 53.3 %, vitamin C-treated group was 80.0 % and vaccinated group was 90.0 %. The results of this study suggest that bath immersion of vitamin C provided an increasing of phagocytic activity (non-specific immune responses), titre antibody (specific immune responses) and protection against A. salmonicida infection in grouper. A. salmonicida vaccine also en-hanced titre antibody and protection against A. salmonicida infection in grouper.
ANALISIS KERAGAMAN GENETIK PADA IKAN KERAPU MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP PENYAKIT YANG DISEBABKAN BAKTERI Vibrio alginolyticus Triana, St. Hidayah; Gani, Mahir S.; Malina, Asmi Citra
Torani Journal of Fisheries and Marine Science Vol. 24 No. 2 (2014)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.149 KB) | DOI: 10.35911/torani.v24i2.227

Abstract

The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) inorder to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrioalginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used forPCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, danUBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higherpolymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 %and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptiblegroups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupersproduced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fishand population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fishwas 0.7032. Keywords: Genetik diversity, Epinephelus fuscoguttatus, V. alginolyticus, PCR, RAPD.
Co-Authors Asmi Citra Malina Asmi Citra Malina, Asmi Citra Hilal Anshary Ilmiah, - Mahir S. Gani, Mahir S. Rantetondok, Alex Rantetondok, Alex