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All Journal Torani Journal of Fisheries and Marine Science AGROTEK: Jurnal Ilmiah Ilmu Pertanian
Mahir S. Gani, Mahir S.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Social Sciences

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EFEKTIVITAS KONSENTRASI Trichoderma sp TERHADAP (Phytopthora palmivora Butl.) PADA BUAH KAKAO (Theobroma cacao L.) Hastuti, A. Rini; Parawansa, Ayu Kartini; Gani, Mahir S.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian Vol 9, No 1 (2025): Maret
Publisher : Percetakan Umi Toaha Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/agrotek.v9i1.708

Abstract

This study was conducted to determine the effective concentration of Trichoderma sp. against Phytophora palmivora. This study was conducted in the disease laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Hasanuddin University. This study was carried out from March to August 2024. The method used in this study was the Detached pod test method, namely looking at cocoa fruit that had been infected with Phytopthora palmivora fungus consisting of five treatment levels T0 (Control), T1 (giving concentration 105), T2 (giving concentration 106), T3 (giving concentration 107), T4 (giving concentration 108). The parameters observed were the area of Phytopthora palmivora fungal spots on the surface of cocoa fruit and identification of the results of cocoa fruit isolation. The results showed that the treatment with a concentration of 106 had an average area of spots with a value of 8.73 mm2, which was the lowest value compared to other treatments. Treatment with a concentration of 106 showed effective control in inhibiting the growth of Phytophora palmivora and can be considered as an alternative in disease control strategies.
ANALISIS KERAGAMAN GENETIK PADA IKAN KERAPU MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP PENYAKIT YANG DISEBABKAN BAKTERI Vibrio alginolyticus Triana, St. Hidayah; Gani, Mahir S.; Malina, Asmi Citra
Torani Journal of Fisheries and Marine Science Vol. 24 No. 2 (2014)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.149 KB) | DOI: 10.35911/torani.v24i2.227

Abstract

The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) inorder to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrioalginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used forPCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, danUBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higherpolymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 %and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptiblegroups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupersproduced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fishand population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fishwas 0.7032. Keywords: Genetik diversity, Epinephelus fuscoguttatus, V. alginolyticus, PCR, RAPD.
Co-Authors Asmi Citra Malina, Asmi Citra Ayu Kartini Parawansa Hastuti, A. Rini St. Hidayah Triana, St. Hidayah