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All Journal Torani Journal of Fisheries and Marine Science
Elmi Nurhaidah Zainuddin, Elmi Nurhaidah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Social Sciences

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EFEKTIVITAS EKSTRAK BIJI JINTAN HITAM (Nigella sativa Linn.) TERHADAP INFEKSI BAKTERI STREPTOCOCCUS AGALACTIAE PADA IKAN NILA (Oreochromis niloticus Linn.) Gustiana, Gustiana; Rantetondok, Alexander; Zainuddin, Elmi Nurhaidah
Torani Journal of Fisheries and Marine Science Vol. 25 No. 1 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.149 KB) | DOI: 10.35911/torani.v25i1.258

Abstract

Black Cumin Seeds (Nigella sativa L.), commonly known as black seed, have been used in traditional medicine.Streptococcus agalactiae is a Gram-positive bacteria that causes the disease Streptococcosis in nile tilapia(Oreochromis niloticus L.). This study aims to find the bioactivity of black cumin seeds (N. sativa L.) againstbacterial pathogens in nile tilapia (O. niloticus L.). The research activities include preparation of samples blackcumin seeds (N. sativa L.), extraction with kinetic maceration method using solvents from different polarity (nhexane,ethanol and methanol), determination of antibacterial activity and minimum inhibition concentration(MIC) by agar diffusion method. The results of antibacterial activity against S. agalactiae of pathogenic bacteriashowed that the highest activity was performed by semi-polar extract ethanol (inhibition zone diameter of 15.33mm), followed by extract polar methanol with inhibition zone diameter of 12.67 mm. Extract non-polar nhexanedid not show any activities against the bacterial test. Determination of the minimum inhibitoryconcentration (MIC) of ethanol extract against S. agalactiae showed a value of 250 ppm. Keywords: Antibacteria, Streptococcus agalactiae, Nile Tilapia (Oreochromis niloticus L.), Nigella Sativa L
Pengaruh Preparasi Sampel, Lama Ekstraksi, dan Presipitan Terhadap Karakteristik Karaginan yang Diekstraksi dari Kappaphycus alvarezii Horisanto, Abraham; Metusalach, Metusalach; Zainuddin, Elmi Nurhaidah; Wijayanti, Willy; Pamungkas, Ilham Wahyu; Agustin, Venny; Jannah, Syerina Raihatul
Torani Journal of Fisheries and Marine Science Vol. 8 No. 2 (2025): VOLUME 8, NOMOR 2, JUNE 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/torani.v8i2.43915

Abstract

Carrageenan is a polysaccharide compound product produced from carrageenophyte seaweed extraction, one of which is Kappaphycus alvarezii. The yield and other characteristics are conventionally produced from an extraction process and have different results. This study aims to explore and optimize the characteristics of carrageenan produced by treating sample preparation with two types of precipitants at specific concentrations, setting the timing during the extraction process, and examining the interaction of these three treatments on the carrageenan characteristics. The results obtained showed that the yield value, acid-insoluble ash (AIA) content, and viscosity were influenced by the preparation method, specifically by chopping the seaweed before extraction. Additionally, the characteristics of the gel strength were influenced by the preparation method, specifically by blending the seaweed. The use of KCl precipitant affects the water content, AIA content, and viscosity, while the CaCl₂ precipitant affects the yield value and gel strength. 1-hour extraction process affected the characteristics of carrageenan for AIA content and gel strength, while extraction for 2 hours affected the yield value, water content, and viscosity. The interaction of 8% KOH solvent by cutting sample treatment, extracted for 2 hours and using KCl precipitant, produced good carrageenan characteristics for water content, AIA content, and viscosity.
Co-Authors Agustin, Venny Alexander Rantetondok, Alexander Gustiana Gustiana, Gustiana Horisanto, Abraham Metusalach Metusalach, Metusalach Pamungkas, Ilham Wahyu Syerina Raihatul Jannah Willy Wijayanti