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EFEKTIVITAS EKSTRAK BIJI JINTAN HITAM (Nigella sativa Linn.) TERHADAP INFEKSI BAKTERI STREPTOCOCCUS AGALACTIAE PADA IKAN NILA (Oreochromis niloticus Linn.) Gustiana, Gustiana; Rantetondok, Alexander; Zainuddin, Elmi Nurhaidah
Torani Journal of Fisheries and Marine Science Vol. 25 No. 1 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.149 KB) | DOI: 10.35911/torani.v25i1.258

Abstract

Black Cumin Seeds (Nigella sativa L.), commonly known as black seed, have been used in traditional medicine.Streptococcus agalactiae is a Gram-positive bacteria that causes the disease Streptococcosis in nile tilapia(Oreochromis niloticus L.). This study aims to find the bioactivity of black cumin seeds (N. sativa L.) againstbacterial pathogens in nile tilapia (O. niloticus L.). The research activities include preparation of samples blackcumin seeds (N. sativa L.), extraction with kinetic maceration method using solvents from different polarity (nhexane,ethanol and methanol), determination of antibacterial activity and minimum inhibition concentration(MIC) by agar diffusion method. The results of antibacterial activity against S. agalactiae of pathogenic bacteriashowed that the highest activity was performed by semi-polar extract ethanol (inhibition zone diameter of 15.33mm), followed by extract polar methanol with inhibition zone diameter of 12.67 mm. Extract non-polar nhexanedid not show any activities against the bacterial test. Determination of the minimum inhibitoryconcentration (MIC) of ethanol extract against S. agalactiae showed a value of 250 ppm. Keywords: Antibacteria, Streptococcus agalactiae, Nile Tilapia (Oreochromis niloticus L.), Nigella Sativa L
BAKTERI ASSOSIASI DI KARANG BATU (Skleractinian) YANG TERINFEKSI PENYAKIT TUMOR (Growth Anomalies) YANG BERASAL DARI PULAU SALEMO KABUPATEN PANGKEP Massinai, Arniati; Tahir, Akbar; Jompa, Jamaluddin; Rantetondok, Alexander
Jurnal Ilmu Kelautan SPERMONDE VOLUME 3 NUMBER 1, 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v3i1.2126

Abstract

 Coral disease is one of the threats to the health of coral but is not yet widely known. This study was conducted todetermine the types of bacterial associations in hard corals infected with growth anomaly (GA). Coral samplesinfected with GA disease were taken by using SCUBA tool on Salemo Island, Mattiro Bombang Village, PangkepDistrict. Bacterial isolation was performed by removing mucus from corals infected by growth anomaly by 1ml and diluted to dilution 10-3 . Purification of bacteria is done by inoculating the solid medium of Marine Agar.Furthermore, Gram staining and biochemical tests were performed. Bacterial identification was performed basedon colony morphology and biochemical reaction test results. The results showed that GA disease can infectcoral Porites, Acropora digitate, Platygyra and pocillopora . Bacteria are found from the genus Flavobacterium,Acinetobacter  and, Neiseria. Keywords: Bacteria association, coral stone, coral disease, growth anomaly