Indijarto Budi Rahardjo, Indijarto Budi
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Efficacy of Selected Plant Extracts to Control Leaf Miner (Lyriomyza spp.) in Chrysanthemum Rahardjo, Indijarto Budi; Marwoto, Budi; Budiarto, Kurniawan
AGRIVITA, Journal of Agricultural Science Vol 42, No 1 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v42i1.2219

Abstract

The use of eco-friendly methods in controlling pest and diseases has become an increasing concern in production system in many economically important crops, including ornamentals. The application of plant extract that has an insecticidal effect is considered as one promising alternative in reducing the negative effects of synthetic pesticides. The research was conducted to assess selected plant extracts in several concentrations against leaf miner (Lyriomyza spp.) in chrysanthemum. The experiment was carried out from January to December 2017 under plastic house conditions at the Indonesian Ornamental Crops Research Institute (IOCRI). The extracted organ of insecticidal plants regularly sprayed into chrysanthemum plants and compared with water as the controls. The results showed that the application of insecticidal plants extracts reduced attack intensity and incidence from water treatment (control). Certain treatment combinations, Chinese mahogany leaf extract at the concentration of 0.25%, C. pyrethrum petal 0.15 and 0.30%, and chinaberry leaf at 0.3, 0.35% even had 0.9-3.13% lesser attack incidences than commercial botanical insecticide Neem Plus. The respected treatments also supressed more than 62% leaf miner attacks and induced the increase of marketable flowers.
Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Diningsih, Erniawati; Aryantika, Safani; Rahardjo, Indijarto Budi; Nuryani, Wakiah; Hanudin; Manzila, Ifa
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.196-205

Abstract

Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Chrysanthemum stunt viroid has been widely reported to be a limiting factor in the production of chrysanthemum cut flowers. The aim of this study was to determine the effect of low temperature treatment (5 ℃) and antiviral ribavirin on the growth and elimination of Chrysanthemum stunt viroid (CSVd) in chrysanthemum plantlets cultured from shoot tips. CSVd infected plants were obtained from a government experimental garden in Cianjur, West Java. The research was carried out in several stages, namely 1) plant sampling, 2) viroid detection using RT-PCR method, 3) initiation of explants and propagation of plant material in vitro, 4) low temperature treatment 5 ℃ with three levels of incubation time at 1, 3, and 5 months, 5) ribavirin antiviral treatment with three levels of concentration (25, 100, and 125 ppm), and 6) confirmation of viroid-free plant materials by RT-PCR. The results showed that low temperature storage and ribavirin treatment reduced the rate of growth of shoot height and number of leaves. Ribavirin did not cause phytotoxity up to concentration level of 125 ppm, although it caused inhibition on shoot growth and leaf number. However, the combination of low temperature treatment (1, 3, and 5 months) with ribavirin (25, 100, and 125 ppm) was not able to eliminate CSVd from tissues at all treatment levels.