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All Journal Jurnal Fitopatologi Indonesia BERITA BIOLOGI Makara Journal of Science
Ifa Manzila
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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EKSPLORASI DAN KARAKTERISASI ENTOMOPATOGEN ASAL BERBAGAI INANG DAN LOKASI [Exploration and Characterization of Entomopathogenic from Various Host and Location] Priyatno, Tri Puji; Samudra, I Made; Manzila, Ifa; Susilowati, Dwi Ningsih; Suryadi, Yadi
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3631.714 KB) | DOI: 10.14203/beritabiologi.v15i1.2859

Abstract

Microbial groups of entomopathogenic (fungi and bacteria) had been reported causing insect mortality. The aim of the study was to explore and characterized entomopathogenic from various host and locations. Fungal identification at genus and species level was caried out based on conidial morphology, hyphal growth, conidiophore and colony color; whilst for bacterial identification was based on standard Bergey’s manual for determinative bacteria. Sixteen entomopathogenic isolates that consisted of fungal and bacteria have been collected and preserved for further characterization. Of the 16 entomopathogen collected samples, five fungal genera was found i.e. Paecilomyces; Metarhizium, Beauveria, Hirsutella; and Cordyceps. Seven isolates belonging to six fungal isolates, and one bacterial isolate had been identified based upon ITS and 16S rDNA sequences, respectively. We confirmed that 6 fungal isolates belong to species of Paecilomyces reniformis, B. bassiana, M. anisopliae, M. anisopliae var acridum, Hirsutella thomsonii. One isolate of red pigmented bacteria Sm201102 have been identified was belonging to species Seratia marcescence. It was also obtained two fungal isolates from different host (spider and beetle) which confirmed by morphological character belong to Cordyceps sp.
KARAKTERISASI ß-1,3-1,4-GLUKANASEBAKTERI ENDOFITIK BURKHOLDERIA CEPACIA ISOLATE76 ASAL TANAMAN PADI Manzila, Ifa; Priyatno, Tri Puji; Fathin, Muhammad Faris; Ambarsari, Laksmi; Suryadi, Yadi; Samudera, I Made; Susilowati, Dwi Ningsih
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1819

Abstract

Pathogenic fungus is one of the constraints to increase crop production. Chemical control using fungicides caused negative effects either to the environment or increased pathogen resistance to fungicide. Biological control using microbial-producing ß glucanase is an alternative method to inhibit the growth of pathogenic fungus. The aim of this study was to characterize ß-1,3-1,4-glucanase produced by rice endophytic bacterium, B. cepacia E76. Purification was carried out by ammonium sulphate precipitation, dialysis, and ion exchange chromatography using DEAE sepharose Fast Flow. A further characteristic of the enzyme activity was studied using oatmeal-glucan substrate.Results showed that precipitation using saturated 80% ammonium sulphate generated a good yield with the purity increased by 11 fold and yield of 66%.After chromatography step, the ß-1,3-1,4-glucanase of B. cepacia was successfully purified with an increasedof purity up to 33 fold and yield of 4%. Based on 10% SDS-PAGE, the enzyme profiles had the molecular weight of 15, 48 and 55 kDa.Of the three isozymes, only the 48 kDa isozyme showed the strongest glucanase activity when grown on media containing glucan as substrate.
The Use of Molecular Markers to Analyze the Genetic Diversity of Indonesian Pepper (Capsicum spp.) Varieties Based on Anthracnose Resistance Nugroho, Kristianto; Terryana, Rerenstradika T.; Manzila, Ifa; Priyatno, Tri Puji; Lestari, Puji
Makara Journal of Science Vol. 23, No. 3
Publisher : UI Scholars Hub

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Abstract

Anthracnose is an important disease affecting the pepper plant and can lead to significant decreases in harvest yield. In this study, the genetic diversity of Indonesian pepper varieties was analyzed based on anthracnose resistance using molecular markers. DNA collected from 15 pepper varieties belonging to two species—Capsicum annuum L. and C. frutescens L.—was amplified using 14 molecular markers. The fungal isolate Colletotrichum capsici was inoculated into ripe harvested pepper fruits to observe their resistance to anthracnose as indicated by lesion size. Phylogenetic analysis revealed that the 15 pepper varieties could be classified into two major clusters with a genetic similarity coefficient of 0.63, and the pepper varieties exhibited varying degrees of resistance to anthracnose based on lesion size. Using the molecular markers, we were able to differentiate the species of pepper varieties, but not their resistance to anthracnose. All markers used in this study were confirmed to be highly informative (PIC > 0.5), suggesting their potential use in genetic studies on peppers. The marker GPMS29 was found to be significantly associated (P < 0.05) with anthracnose resistance. This information about the genetic diversity of peppers—along with the molecular markers used in our study—could prove to be useful in the further development of breeding programs of pepper plants in terms of anthracnose resistance in Indonesia.
Resistance of Several Local Rice Varieties to Isolate Tungro Virus from Muara Ruimassa, Reymas; Manzila, Ifa; Temaja, I Gede Rai Maya; Sudana, I Made
Jurnal Fitopatologi Indonesia Vol 18 No 1 (2022): Januari 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.1.1-8

Abstract

Resistance of Several Local Rice Varieties to Isolate Tungro Virus from Muara Tungro disease is one of the important diseases in rice because it causes stunted plants and may induce significant yield losses. Planting resistant varieties is a recommended control strategy to overcome tungro disease. The research was conducted to find sources of genetic resistance from local rice varieties that can be used in breeding of tungro disease resistant varieties. The experiment was arranged in a descriptive research design with observational techniques. Observations were made on 16 local rice varieties with each variety consisting of 30 sample plants so that the number of plants used was 480 plants. The isolate of tungro virus collected from rice field in Muara, Bogor was inoculated to the test plants through the insect vector, green leafhopper (Nepothettix virescens). The results showed that the resistance of local varieties varied based on the value of disease index, disease incidence and plant height reduction. ‘TN1’ was the check susceptible variety against tungro virus, while the local rice varieties were differentiated into resistant (‘Utri Merah’), moderate (‘Karau’, ‘Bekongan’, ‘Bujang Bernai’, ‘Srogel Abang’, ‘Pulut Garu’, ‘Randu Range’, ‘Siredep’, ‘Katimpung’, ‘Pulut Sawijan’, ‘Dube’), moderate tend to be susceptible (‘Blumbungan’, ‘Tjere bandung’, ‘Sempor’) and susceptible (‘Rumbai’). All varieties can be infected (disease incidence 27 – 76%), but disease severity varies greatly (3–7). The more severe the disease, the higher the plant height reduction. ‘Srogel Abang’ is a variety that is useful for breeding rice varieties resistant to tungro disease because it showed moderate response with a low percentage of plant height reduction (36%) so that plant productivity can still be maintained.
Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Diningsih, Erniawati; Aryantika, Safani; Rahardjo, Indijarto Budi; Nuryani, Wakiah; Hanudin; Manzila, Ifa
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.196-205

Abstract

Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Chrysanthemum stunt viroid has been widely reported to be a limiting factor in the production of chrysanthemum cut flowers. The aim of this study was to determine the effect of low temperature treatment (5 ℃) and antiviral ribavirin on the growth and elimination of Chrysanthemum stunt viroid (CSVd) in chrysanthemum plantlets cultured from shoot tips. CSVd infected plants were obtained from a government experimental garden in Cianjur, West Java. The research was carried out in several stages, namely 1) plant sampling, 2) viroid detection using RT-PCR method, 3) initiation of explants and propagation of plant material in vitro, 4) low temperature treatment 5 ℃ with three levels of incubation time at 1, 3, and 5 months, 5) ribavirin antiviral treatment with three levels of concentration (25, 100, and 125 ppm), and 6) confirmation of viroid-free plant materials by RT-PCR. The results showed that low temperature storage and ribavirin treatment reduced the rate of growth of shoot height and number of leaves. Ribavirin did not cause phytotoxity up to concentration level of 125 ppm, although it caused inhibition on shoot growth and leaf number. However, the combination of low temperature treatment (1, 3, and 5 months) with ribavirin (25, 100, and 125 ppm) was not able to eliminate CSVd from tissues at all treatment levels.
Co-Authors Aryantika, Safani DWI NINGSIH SUSILOWATI Erniawati Diningsih Fathin, Muhammad Faris Hanudin I G. R. M. TEMAJA I Made Samudera, I Made I Made Samudra I Made Sudana Indijarto Budi Rahardjo, Indijarto Budi KRISTIANTO NUGROHO, KRISTIANTO LAKSMI AMBARSARI PUJI LESTARI RERENSTRADIKA T. TERRYANA, RERENSTRADIKA T. Ruimassa, Reymas Tri Puji Priyatno Wakiah Nuryani Yadi Suryadi