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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) BERITA BIOLOGI Makara Journal of Science
Ifa Manzila
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Medicine & Pharmacology

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EKSPLORASI DAN KARAKTERISASI ENTOMOPATOGEN ASAL BERBAGAI INANG DAN LOKASI [Exploration and Characterization of Entomopathogenic from Various Host and Location] Priyatno, Tri Puji; Samudra, I Made; Manzila, Ifa; Susilowati, Dwi Ningsih; Suryadi, Yadi
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3631.714 KB) | DOI: 10.14203/beritabiologi.v15i1.2859

Abstract

Microbial groups of entomopathogenic (fungi and bacteria) had been reported causing insect mortality. The aim of the study was to explore and characterized entomopathogenic from various host and locations. Fungal identification at genus and species level was caried out based on conidial morphology, hyphal growth, conidiophore and colony color; whilst for bacterial identification was based on standard Bergey’s manual for determinative bacteria. Sixteen entomopathogenic isolates that consisted of fungal and bacteria have been collected and preserved for further characterization. Of the 16 entomopathogen collected samples, five fungal genera was found i.e. Paecilomyces; Metarhizium, Beauveria, Hirsutella; and Cordyceps. Seven isolates belonging to six fungal isolates, and one bacterial isolate had been identified based upon ITS and 16S rDNA sequences, respectively. We confirmed that 6 fungal isolates belong to species of Paecilomyces reniformis, B. bassiana, M. anisopliae, M. anisopliae var acridum, Hirsutella thomsonii. One isolate of red pigmented bacteria Sm201102 have been identified was belonging to species Seratia marcescence. It was also obtained two fungal isolates from different host (spider and beetle) which confirmed by morphological character belong to Cordyceps sp.
KARAKTERISASI ß-1,3-1,4-GLUKANASEBAKTERI ENDOFITIK BURKHOLDERIA CEPACIA ISOLATE76 ASAL TANAMAN PADI Manzila, Ifa; Priyatno, Tri Puji; Fathin, Muhammad Faris; Ambarsari, Laksmi; Suryadi, Yadi; Samudera, I Made; Susilowati, Dwi Ningsih
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1819

Abstract

Pathogenic fungus is one of the constraints to increase crop production. Chemical control using fungicides caused negative effects either to the environment or increased pathogen resistance to fungicide. Biological control using microbial-producing ß glucanase is an alternative method to inhibit the growth of pathogenic fungus. The aim of this study was to characterize ß-1,3-1,4-glucanase produced by rice endophytic bacterium, B. cepacia E76. Purification was carried out by ammonium sulphate precipitation, dialysis, and ion exchange chromatography using DEAE sepharose Fast Flow. A further characteristic of the enzyme activity was studied using oatmeal-glucan substrate.Results showed that precipitation using saturated 80% ammonium sulphate generated a good yield with the purity increased by 11 fold and yield of 66%.After chromatography step, the ß-1,3-1,4-glucanase of B. cepacia was successfully purified with an increasedof purity up to 33 fold and yield of 4%. Based on 10% SDS-PAGE, the enzyme profiles had the molecular weight of 15, 48 and 55 kDa.Of the three isozymes, only the 48 kDa isozyme showed the strongest glucanase activity when grown on media containing glucan as substrate.
The Use of Molecular Markers to Analyze the Genetic Diversity of Indonesian Pepper (Capsicum spp.) Varieties Based on Anthracnose Resistance Nugroho, Kristianto; Terryana, Rerenstradika T.; Manzila, Ifa; Priyatno, Tri Puji; Lestari, Puji
Makara Journal of Science Vol. 23, No. 3
Publisher : UI Scholars Hub

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Abstract

Anthracnose is an important disease affecting the pepper plant and can lead to significant decreases in harvest yield. In this study, the genetic diversity of Indonesian pepper varieties was analyzed based on anthracnose resistance using molecular markers. DNA collected from 15 pepper varieties belonging to two species—Capsicum annuum L. and C. frutescens L.—was amplified using 14 molecular markers. The fungal isolate Colletotrichum capsici was inoculated into ripe harvested pepper fruits to observe their resistance to anthracnose as indicated by lesion size. Phylogenetic analysis revealed that the 15 pepper varieties could be classified into two major clusters with a genetic similarity coefficient of 0.63, and the pepper varieties exhibited varying degrees of resistance to anthracnose based on lesion size. Using the molecular markers, we were able to differentiate the species of pepper varieties, but not their resistance to anthracnose. All markers used in this study were confirmed to be highly informative (PIC > 0.5), suggesting their potential use in genetic studies on peppers. The marker GPMS29 was found to be significantly associated (P < 0.05) with anthracnose resistance. This information about the genetic diversity of peppers—along with the molecular markers used in our study—could prove to be useful in the further development of breeding programs of pepper plants in terms of anthracnose resistance in Indonesia.
Resistance of Several Local Rice Varieties to Isolate Tungro Virus from Muara Ruimassa, Reymas; Manzila, Ifa; Temaja, I Gede Rai Maya; Sudana, I Made
Jurnal Fitopatologi Indonesia Vol. 18 No. 1 (2022): Januari 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.1.1-8

Abstract

Penyakit tungro merupakan salah satu penyakit penting pada tanaman padi karena menimbulkan gejala tanaman kerdil dan mampu menyebabkan kehilangan hasil yang nyata. Penanaman varietas tahan merupakan strategi pengendalian yang direkomendasikan untuk mengatasi penyakit tungro. Penelitian dilakukan untuk mencari sumber ketahanan genetik dari varietas padi lokal yang dapat digunakan dalam perakitan varietas tahan penyakit tungro. Percobaan disusun dalam rancangan penelitian deskriptif dengan teknik observasi. Observasi dilakukan terhadap 16 varietas padi lokal dengan setiap varietas terdiri atas 30 tanaman contoh sehingga jumlah keseluruhan tanaman yang digunakan sebanyak 480 tanaman. Isolat virus tungro dari pertanaman padi di Muara, Bogor diinokulasikan ke tanaman uji melalui serangga vektor wereng hijau (Nephotettix virescens). Hasil penelitian menunjukkan bahwa ketahanan varietas lokal bervariasi berdasarkan nilai indeks penyakit, insidensi penyakit dan penyusutan tinggi tanaman. Varietas TN1 merupakan varietas pembanding dengan reaksi rentan, sedangkan varietas padi lokal dibedakan atas tahan (‘Utri Merah’), moderat (‘Karau’, ‘Bekongan’, ‘Bujang Berinai’, ‘Srogel Abang’, ‘Pulut Garu’, ‘Randu Kisaran’, ‘Siredep’, ‘Katimpung’, ‘Pulut Sawijan’, ‘Dube’), moderat cenderung rentan (‘Blumbungan’, ‘Tjere bandung’, ‘Sempor’) dan rentan (‘Rumbai’). Semua varietas dapat terinfeksi (insidensi penyakit 27–76%), tetapi indeks keparahan penyakit sangat bervariasi (3–7). Semakin berat keparahan penyakit menyebabkan penyusutan tinggi tanaman semakin tinggi. ‘Srogel Abang’ merupakan varietas yang bermanfaat untuk perakitan varietas padi unggul tahan penyakit tungro karena bersifat moderat dengan persentase penyusutan tinggi tanaman yang rendah (36%) sehingga produktivitas tanaman masih dapat dipertahankan.
Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Diningsih, Erniawati; Aryantika, Safani; Rahardjo, Indijarto Budi; Nuryani, Wakiah; Hanudin; Manzila, Ifa
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.196-205

Abstract

Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Chrysanthemum stunt viroid has been widely reported to be a limiting factor in the production of chrysanthemum cut flowers. The aim of this study was to determine the effect of low temperature treatment (5 ℃) and antiviral ribavirin on the growth and elimination of Chrysanthemum stunt viroid (CSVd) in chrysanthemum plantlets cultured from shoot tips. CSVd infected plants were obtained from a government experimental garden in Cianjur, West Java. The research was carried out in several stages, namely 1) plant sampling, 2) viroid detection using RT-PCR method, 3) initiation of explants and propagation of plant material in vitro, 4) low temperature treatment 5 ℃ with three levels of incubation time at 1, 3, and 5 months, 5) ribavirin antiviral treatment with three levels of concentration (25, 100, and 125 ppm), and 6) confirmation of viroid-free plant materials by RT-PCR. The results showed that low temperature storage and ribavirin treatment reduced the rate of growth of shoot height and number of leaves. Ribavirin did not cause phytotoxity up to concentration level of 125 ppm, although it caused inhibition on shoot growth and leaf number. However, the combination of low temperature treatment (1, 3, and 5 months) with ribavirin (25, 100, and 125 ppm) was not able to eliminate CSVd from tissues at all treatment levels.
Polymorphics SSR Markers of Chilli Parental and Breeding Lines in Chilli Resistance to Pepper Yellow Leaf Curl Virus (PYLCV) Manzila, Ifa; Syukur, M.; Priyatno, Tri Puji; Reflinur; Azmi, Chotimatul; Wulandari, Astri Widia; Gunaeni, Neni; Azizah, Nur
Jurnal Hortikultura Indonesia (JHI) Vol. 12 No. 2 (2021): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.12.2.126-137

Abstract

Aksesi cabai IPBC12 telah diketahui memiliki gen ketahanan dominan terhadap PYLCV dan dapat dimanfaatkan sebagi donor gen untuk perakitan varietas cabai tahan PYLCV. PYLCV merupakan salah satu virus patogen penting pada pertanaman cabai di Indonesia. Identifikasi marka SSR polimorfik pada populasi persilangan antara IPBC12 dan varietas Yuni dilakukan untuk mendapatkan marka yang dapat digunakan untuk seleksi progeni hasil persilangan dan terpaut dengan sifat ketahanan terhadap PYLCV. Sebanyak 20 marka SSR dianalisis polimorfismenya pada dua tetua persilangan, kemudian marka yang polimorfik diuji pada galur generasi F1 dan F2. Hasil penelitian menunjukkan ada empat marka polimorfik pada kedua tetua persilangan, tetapi ketika diuji pada galur-galur keturunannya hanya 3 marka (CaBR61, CaBR64, dan CaBR98) yang polimorfik. Berdasarkan analisis marka, 14 galur F1 terkonfirmasi hasil persilangan antar aksesi IPBC12 dan varietas Yuni. Marka yang secara konsisten mendeteksi penurunan alel dari kedua tetua pada progeni F1 adalah CaBR61. Marka tersebut berpotensi sebagai marka seleksi galur-galur hasil persilangan pada tanaman cabai. Analisis molekuler pada galur-galur F2 tidak mendapatkan keterpautan antara marka dengan sifat ketahanan. Perlu analisis lebih lanjut menggunakan jumlah marka yang mencukupi dan tersebar merata dalam genom cabai untuk memetakan gen ketahanan terhadap PYLCV pada populasi persilangan antara aksesi IPBC12 dan varietas Yuni. Kata kunci: aksesi IPBC12, Capsicum annuum, seleksi berpandu marka, varietas Yuni
Pengaruh Perlakuan Ethyl Methane Sulfonate pada Tanaman Cabai (Capsicum annuum L.) dan Ketahanannya terhadap Chilli Veinal Mottle Virus (ChiVMV) Manzila, Ifa; Hidayat, Sri Hendrastuti; Mariska, Ika; Sujiprihati, Sriani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.474 KB) | DOI: 10.24831/jai.v38i3.14250

Abstract

Ethyl Methane Sulfonate (EMS) may induce mutation leading to somaclonal variation if it is used at the appropriate combination of EMS concentration and exposure time. Variation in somaclonal might be valuable as a source of resistance to plant pathogens including plant viruses. This study was aimed 1) to determine the optimum EMS concentration and incubation time that may induce somaclonal variation in chilli pepper; and 2) to evaluate the resistance of the somaclone to ChiVMV infection. Shoot-tip explants of fi ve chilli pepper genotypes (Jatilaba, ICPN 12#4, PBC495, Helem, and Gelora) were treated with EMS at combination of different concentrations (0.25%, 0.5% 1.0% and control), and incubation time (15, 30, 60 min). Subsequently, each explant was grown in multiplication media (MS media + 5 mg L -1 BAP + 0.5 mg L-1 TDZ), rooting media (MS media + 1 mg L-1 NAA), and acclimatization media (mixture of soil : sand : compost 2:1:1 w/w). Our results showed that the higher EMS concentration and the longer incubation period the smaller the number of survive explants. The highest survival rate 20.4 % was achieved with 0.5% EMS in combination with 60 min incubation period. This treatment combination also showed induction of phenotypic variation. Two somaclonal plants derived from Gelora genotype, designated as somaclones K1 and K2, survived until fruit development and maturation. A total of 245 progenies of K1 and 243 progenies of K2, respectively were evaluated for their resistance to ChiVMV infection through mechanical inoculation using ChiVMV-Cikabayan isolate. Following the detection of ChiVMV using DAS-ELISA, it was confi rmed that 4.09% of the somaclonal progenies were resistance to ChiVMV. Keywords: Capsicum annuum L., ChiVMV, ethyl methane sulfonate, induce mutation, resistance
Co-Authors Aryantika, Safani Chotimatul Azmi, Chotimatul DWI NINGSIH SUSILOWATI Erniawati Diningsih Fathin, Muhammad Faris Hanudin I G. R. M. TEMAJA I Made Samudera, I Made I Made Samudra I Made Sudana Ika Mariska Indijarto Budi Rahardjo, Indijarto Budi KRISTIANTO NUGROHO, KRISTIANTO LAKSMI AMBARSARI M. Syukur Neni Gunaeni Nur Azizah PUJI LESTARI Reflinur RERENSTRADIKA T. TERRYANA, RERENSTRADIKA T. Ruimassa, Reymas SRI HENDRASTUTI HIDAYAT SRIANI SUJIPRIHATI Tri Puji Priyatno Wakiah Nuryani Wulandari, Astri Widia Yadi Suryadi