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Hanudin
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Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Diningsih, Erniawati; Aryantika, Safani; Rahardjo, Indijarto Budi; Nuryani, Wakiah; Hanudin; Manzila, Ifa
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.196-205

Abstract

Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Chrysanthemum stunt viroid has been widely reported to be a limiting factor in the production of chrysanthemum cut flowers. The aim of this study was to determine the effect of low temperature treatment (5 ℃) and antiviral ribavirin on the growth and elimination of Chrysanthemum stunt viroid (CSVd) in chrysanthemum plantlets cultured from shoot tips. CSVd infected plants were obtained from a government experimental garden in Cianjur, West Java. The research was carried out in several stages, namely 1) plant sampling, 2) viroid detection using RT-PCR method, 3) initiation of explants and propagation of plant material in vitro, 4) low temperature treatment 5 ℃ with three levels of incubation time at 1, 3, and 5 months, 5) ribavirin antiviral treatment with three levels of concentration (25, 100, and 125 ppm), and 6) confirmation of viroid-free plant materials by RT-PCR. The results showed that low temperature storage and ribavirin treatment reduced the rate of growth of shoot height and number of leaves. Ribavirin did not cause phytotoxity up to concentration level of 125 ppm, although it caused inhibition on shoot growth and leaf number. However, the combination of low temperature treatment (1, 3, and 5 months) with ribavirin (25, 100, and 125 ppm) was not able to eliminate CSVd from tissues at all treatment levels.
Pelatihan Metode Dakwah Pada Kalangan Mubaligh Dan Ormas Islam Kota Baubau: Pengabdian Rahmat Haniru; Safaruddin Yahya; Darmayanti; Madi; Muh Ridwan; La Jidi; Hanudin; Muh. Zaidir; Abdul Kahar Syarifuddin; Muammar; Armed Tahyas; Siti Salmawati Hada. N
Jurnal Pengabdian Masyarakat dan Riset Pendidikan Vol. 4 No. 1 (2025): Jurnal Pengabdian Masyarakat dan Riset Pendidikan Volume 4 Nomor 1 (Juli 2025 -
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jerkin.v4i1.2619

Abstract

Penelitian pengabdian kepada masyarakat ini bertujuan memberikan pemahaman dan penguatan kepada para muballigh dan ormas Islam kota Baubau terkait kompetensi metode dakwah yang efektif dengan mengacu pada prinsip-prinsip dan etika dakwah Islam. Perlunya para muballigh memahami literasi Al Quran dan metode dakwah Nabi Muhammad dan mengimplementasikan metode dakwah dengan pendekatan kontekstual dengan merujuk pada karakteristik pendengar yang beragam. Perkembangan zaman dan kemajuan teknologi sebagai bagian dari keniscayaan menjadi tuntutan untuk para muballigh menyesuaikan diri memilih tema, konten, metode bahkan media yang harus digunakan agar materi dakwah menjadi menarik. Hasil pelatihan ini menunjukkan adanya antusias dari peserta pelatihan dan pemahaman baru terkait metode-metode dakwah yang inovatif dan kontekstual pada masa kekinian, terutama para muballig di era modern dipandang perlu dapat menyesuaikan diri dengan perkembangan tekhnologi, agar pesan-dakwah tidak hanya disampaikan pada ruang-ruang publik tetapi dapat disampaikan di ruang online.
Co-Authors Abdul Kahar Syarifuddin Armed Tahyas Aryantika, Safani Darmayanti Erniawati Diningsih Ifa Manzila Indijarto Budi Rahardjo, Indijarto Budi La Jidi Madi Muammar Muh Ridwan Muh. Zaidir Rahmat Haniru Safaruddin Yahya Siti Salmawati Hada. N Wakiah Nuryani