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PENGARUH AUKSIN DAN SITOKININ TERHADAP PERBANYAKAN MIKRO TANAMAN BINAHONG (Anredera cordifolia (Tenore) Steenis) Suparjo, .; Royani, Juwartina Ida; Rosmalawati, Syofi; Tajuddin, Teuku; Riyadi, Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 2 (2016): December 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

EFFECT OF AUXIN AND CYTOKININ ON MICROPROPAGATION OF BINAHONG (AnrederaÂ cordifoliaÂ (Tenore) Steenis)Binahong (Anredera cordifolia (Tenore) Steenis) is known as miraculous medicinal plant for its potential to cure for various kinds of diseases such as diabetes, stabilizing blood pressure and circulation, accelerating wound healing, and preventing stroke. In order to provide high quality seedlings of this medicinal plant continuously in large amount, the study on binahong micropropagation was performed. Plant growth regulators of auxins and cytokinins were applied in single or in combination so as to observe their effect on the growth of binahong explants. The results showed that 2,4-D induced callus formation in large diameter on all treatments. Nevertheless, this plant growth regulator had a negative effect on growth and development of shoot and leaves. In the combination treatments between IAA and BAP, it revealed that the higher the concentration of BAP in the media, the lower the number of leaves initiated on shoot explants. Increasing the concentration of IAA upto 1.5 ppm influenced the increasing of shoot tallness and the number of internodes. Our results can be useful for improving the binahong shoot propagation efficiency, as well as callus culture studies.Keywords: Auxin, cytokinin, callus, micropropagation, medicinal plantABSTRAKBinahong (Anredera cordifolia (Tenore) Steenis) dikenal sebagai tanaman obat ajaib karena dapat digunakan untuk pengobatan berbagai macam penyakit seperti diabetes, melancarkan peredaran dan tekanan darah, mempercepat penyembuhan luka, mencegah stroke. Dalam mendukung ketersediaan bibit tanaman obat herbal yang berkualitas secara berkelanjutan maka dilakukan kajian tentang perbanyakan bibit tanaman binahong. Zat pengatur tumbuh auksin dan sitokinin dalam bentuk tunggal maupun kombinasi diaplikasikan pada penelitian ini untuk melihat pengaruhnya terhadap berbagai eksplan binahong. Hasilnya menunjukkan bahwa 2,4-D merangsang pembentukan kalus dengan ukuran yang besar pada semua perlakuan. Namun demikian zat pengatur tumbuh ini memberikan pengaruh negatif terhadap pertumbuhan dan perkembangan tunas dan daun. Dari perlakuan kombinasi zat pengatur tumbuh IAA dan BAP, pertambahan konsentrasi BAP di dalam media menurunkan jumlah daun yang terbentuk pada eksplan pucuk binahong. Demikian pula dengan pertambahan konsentrasi IAA hingga 1,5 ppm sangat berpengaruh terhadap pertumbuhan meninggi tunas dan pertambahan jumlah ruas. Hasil dari studi ini dapat dimanfaatkan untuk studi lanjutan dalam meningkatkan efisiensi perbanyakan tunas serta kultur kalus binahong.Kata kunci: Auksin, sitokinin, kalus, perbanyakan mikro, tanaman obat herbal

ANALISA KANDUNGAN ANDROGRAPHOLIDE PADA TANAMAN SAMBILOTO (Andrographis paniculata) DARI 12 LOKASI DI PULAU JAWA Royani, Juwartina Ida; Hardianto, Dudi; Wahyuni, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 1, No 1 (2014): December 2014
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Concentration of active compounds contained in medicinal plants is determined by genetic factors as well as growth environment. In sambiloto plants both factors have major impacts on the formation of diterpene lactone, andrographolide. Variation of sampling time, cultivation, and processing methods causes variation in the content of active compounds of the same plant. The purpose of this study was to determine andrographolide concentration of sambiloto plants obtained from 12 different locations with various planting conditions in Java Island. andrographolide content of sambiloto was extracted by methanol and analyzed using HPLC. The results showed that the concentrations of andrographolide varied from 0.29 to 4.44% with an average of 2.19% on dry weight basis. The highest concentration of 4.44% was detected in sambiloto accession from Wonokaton Village, Pasuruan Regency while the lowest one was from Conggeang Kulon Village, Sumedang Regency. Three sambiloto accessions had potential to be further developed as their andrographolide concentrations were above 3%, which was higher than those from all the others.Keywords: Andrographis paniculata, andrographolide, active coumpound, HPLC, Java islandÂ ABSTRAKKadar senyawa aktifÂ yang terkandung pada tanaman obat selain dipengaruhi oleh faktor genetik juga dipengaruhi oleh faktor lingkungan tumbuhnya. Pada tanaman sambiloto kedua faktor tersebut berpengaruh sangat besar pada pembentukan diterpen lakton, andrographolide. Adanya variasi pada waktu pengambilan sampel, tempat penanaman, metode pengolahan dan lain sebagainya berakibat pada perbedaan dalam kandungan senyawa aktif pada tanaman yang sama. Tujuan dari penelitian ini adalah untuk mengetahui kadar andrographolide dari tanaman sambiloto yang diambil dari 12 lokasi tumbuh dengan kondisi penanaman yang berbeda di Pulau Jawa. Daun tanaman sambiloto diekstrak dengan methanol kemudian dianalisis kandungan andrographolide menggunakan HPLC. Kadar andrographolide yang dihasilkan bervariasi berkisar antara 0,29-4,44% dengan kadar rata-rata adalah 2,19% berat kering. Kadar tertinggi didapatkan pada aksesi dari Desa Wonokaton Kabupaten Pasuruan dengan kadar andrographolide adalah 4,44% sedangkan kadar yang terendah didapatkan pada aksesi dari Desa Conggeang Kulon, Kab. Sumedang. Berdasarkan data kandungan andrographolide, diperoleh 3 aksesi sambiloto yang potensial untuk dikembangkan menjadi aksesi unggulan karena kadar andrographolidenya di atas 3%, melebihi semua yang lain.Kata kunci: Andrographis paniculata, andrographolide, senyawa aktif, HPLC, pulau Jawa

ISOLASI DAN AMPLIFIKASI DNA KELADI TIKUS (Thyponium flagelliform) UNTUK IDENTIFIKASI KERAGAMAN GENETIK Hikmatyar, Mohamad Fazri; Royani, Juwartina Ida; ., Dasumiati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 2 (2015): December 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Keladi tikusÂ (Typhonium flagelliforme) is one of considerable potential medicinal plants, especially as anticancer herbal medicine. In Indonesia, this plant grows throughout the island of Java, in part of Kalimantan, Sumatra and Papua. The development of Keladi tikus plants to provide raw material to meet public demand is constrained with the quality of the plants that is not standardized yet. DNA marker technique has been widely used for identification of standardization and diversity of varieties. The aims of this research were to isolate DNA from 17 accessions of Keladi tikus from various regions in Indonesia and to amplify the DNA using ISSRÂ primers. The results obtained were 17 accessions of Keladi tikus that had been isolated using the modified CTAB method. Amplifications were done by using SBLT2 and SBLT8 primers that facilitated the appearance of the polymorphism bands on the 17 accessions of Keladi tikus. Thus, SBLT2 and SBLT8 primers can be used to identify genetic variations of Keladi Tikus.Keywords: Typonium flagelliforme, Keladi tikus, ISSR, medicinal plant, amplificationÂ ABSTRAKKeladi tikusÂ (Typonium flagelliforme) merupakan salah satu tanaman obatÂ yang cukup potensial khususnya sebagai obat herbal antikanker. Tanaman ini di Indonesia tersebar di sepanjang Pulau Jawa, sebagian Kalimantan, Sumatera dan Papua. Pengembangan tanaman keladi tikus untuk memenuhi bahan baku kebutuhan masyarakat saat ini terkendala pada mutu tanaman tersebut yang belum terstandar. Teknik penanda DNA telah banyak digunakan untuk standarisasi dan identifikasi keragaman varietas. Tujuan dari penelitian ini adalah untuk mengisolasi DNA dari 17 aksesi Keladi tikus dari berbagai daerah di Indonesia dan mengamplifikasi DNA tersebut dengan primer ISSR. Hasil penelitian menunjukkan bahwa 17 aksesi keladi tikus telah dapat diisolasi dengan menggunakan metode CTAB yang dimodifikasi. Amplifikasi dilakukan dengan primer SBLT2 dan SBLT8 yang mampu memunculkan pita-pita polimorfisme pada ke 17 aksesi Keladi tikus. Primer SBLT2 dan SBLT8 dapat digunakan untuk identifikasi variasi genetic Keladi tikus.Kata Kunci: Keladi tikus, Typonium flagelliforme, ISSR, tanaman obat, amplifikasi

REGENERASI TANAMAN OBAT KELADI TIKUS (Thyponium flagelliform L. Blume) MELALUI EMBRIOGENESIS SOMATIK SECARA IN VITRO royani, juwartina ida; Sulistyorini, indah; Utomo, Dwi Rizkyanto
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 1 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Somatic embryogenesis is a process whereby somatic cells have differentiation in somatic embryos and it’s formed from embryonic cells due to the high polarization of uncontrolled cell division. The aim of this research is to produced shoots of rodent tuber via somatic embryos.Induction of somatic embryos from explants in MS media with N (2-chloro-4-pyridyl)-Nphenyl urea (CPPU) and continuous subculture in MS media without plant growth regulator for 1 month. Plantlet regeneration growth in liquid MS media with BAP and IBA fortified with 40 gr/l of sucrose in the dark. The result showed that somatic embryonic appears in media induction after 2 week and plantlet regeneration in 4 week. All plantlet growed from embryosomatic can survive 100% in field with normal shoot.

PEMANFAATAN BAHAN TEKNIS KNO3, CaCl2, MgSO4, KH2PO4 SEBAGAI HARA MAKRO DAN Benzil Adenin DALAM PERBANYAKAN JATI (Tectona grandis L) SECARA IN VITRO karyanti, karyanti; royani, juwartina ida
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Indonesia is a major producer of teak after India and will gradually decrease if not followed by replanting. In general, teak plants propagated through seeds but have many disadvantages. Teak plant propagation using in vitro technique being an alternative to get the mass production of teak clones. In vitro technique, to some extent, needs a high cost particularly in using pure chemical substance. As an alternative solution, pure chemical substance can be substituted by using a few technique chemical substances such as KNO3, CaCl2, MgSO4, and KH2PO4. The aim of this research was to evaluated induction of shoot teaks, planted on different media with macro element substituted by different chemicals such as KNO3, CaCl2, MgSO4, and KH2PO4. The result showed that using the same concentration between 2 different chemical substances on teak shoot induction, there was no different shoot growing in teak propagated between 2 media.

PENGARUH AUKSIN DAN SITOKININ TERHADAP PERBANYAKAN MIKRO TANAMAN BINAHONG (Anredera cordifolia (Tenore) Steenis) Suparjo, .; Royani, Juwartina Ida; Rosmalawati, Syofi; Tajuddin, Teuku; Riyadi, Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

EFFECT OF AUXIN AND CYTOKININ ON MICROPROPAGATION OF BINAHONG (AnrederaÃ‚Â cordifoliaÃ‚Â (Tenore) Steenis)Binahong (Anredera cordifolia (Tenore) Steenis) is known as miraculous medicinal plant for its potential to cure for various kinds of diseases such as diabetes, stabilizing blood pressure and circulation, accelerating wound healing, and preventing stroke. In order to provide high quality seedlings of this medicinal plant continuously in large amount, the study on binahong micropropagation was performed. Plant growth regulators of auxins and cytokinins were applied in single or in combination so as to observe their effect on the growth of binahong explants. The results showed that 2,4-D induced callus formation in large diameter on all treatments. Nevertheless, this plant growth regulator had a negative effect on growth and development of shoot and leaves. In the combination treatments between IAA and BAP, it revealed that the higher the concentration of BAP in the media, the lower the number of leaves initiated on shoot explants. Increasing the concentration of IAA upto 1.5 ppm influenced the increasing of shoot tallness and the number of internodes. Our results can be useful for improving the binahong shoot propagation efficiency, as well as callus culture studies.Keywords: Auxin, cytokinin, callus, micropropagation, medicinal plantABSTRAKBinahong (Anredera cordifolia (Tenore) Steenis) dikenal sebagai tanaman obat ajaib karena dapat digunakan untuk pengobatan berbagai macam penyakit seperti diabetes, melancarkan peredaran dan tekanan darah, mempercepat penyembuhan luka, mencegah stroke. Dalam mendukung ketersediaan bibit tanaman obat herbal yang berkualitas secara berkelanjutan maka dilakukan kajian tentang perbanyakan bibit tanaman binahong. Zat pengatur tumbuh auksin dan sitokinin dalam bentuk tunggal maupun kombinasi diaplikasikan pada penelitian ini untuk melihat pengaruhnya terhadap berbagai eksplan binahong. Hasilnya menunjukkan bahwa 2,4-D merangsang pembentukan kalus dengan ukuran yang besar pada semua perlakuan. Namun demikian zat pengatur tumbuh ini memberikan pengaruh negatif terhadap pertumbuhan dan perkembangan tunas dan daun. Dari perlakuan kombinasi zat pengatur tumbuh IAA dan BAP, pertambahan konsentrasi BAP di dalam media menurunkan jumlah daun yang terbentuk pada eksplan pucuk binahong. Demikian pula dengan pertambahan konsentrasi IAA hingga 1,5 ppm sangat berpengaruh terhadap pertumbuhan meninggi tunas dan pertambahan jumlah ruas. Hasil dari studi ini dapat dimanfaatkan untuk studi lanjutan dalam meningkatkan efisiensi perbanyakan tunas serta kultur kalus binahong.Kata kunci: Auksin, sitokinin, kalus, perbanyakan mikro, tanaman obat herbal

ANALISA KANDUNGAN ANDROGRAPHOLIDE PADA TANAMAN SAMBILOTO (Andrographis paniculata) DARI 12 LOKASI DI PULAU JAWA Royani, Juwartina Ida; Hardianto, Dudi; Wahyuni, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Concentration of active compounds contained in medicinal plants is determined by genetic factors as well as growth environment. In sambiloto plants both factors have major impacts on the formation of diterpene lactone, andrographolide. Variation of sampling time, cultivation, and processing methods causes variation in the content of active compounds of the same plant. The purpose of this study was to determine andrographolide concentration of sambiloto plants obtained from 12 different locations with various planting conditions in Java Island. andrographolide content of sambiloto was extracted by methanol and analyzed using HPLC. The results showed that the concentrations of andrographolide varied from 0.29 to 4.44% with an average of 2.19% on dry weight basis. The highest concentration of 4.44% was detected in sambiloto accession from Wonokaton Village, Pasuruan Regency while the lowest one was from Conggeang Kulon Village, Sumedang Regency. Three sambiloto accessions had potential to be further developed as their andrographolide concentrations were above 3%, which was higher than those from all the others.Keywords: Andrographis paniculata, andrographolide, active coumpound, HPLC, Java islandÃ‚Â ABSTRAKKadar senyawa aktifÃ‚Â yang terkandung pada tanaman obat selain dipengaruhi oleh faktor genetik juga dipengaruhi oleh faktor lingkungan tumbuhnya. Pada tanaman sambiloto kedua faktor tersebut berpengaruh sangat besar pada pembentukan diterpen lakton, andrographolide. Adanya variasi pada waktu pengambilan sampel, tempat penanaman, metode pengolahan dan lain sebagainya berakibat pada perbedaan dalam kandungan senyawa aktif pada tanaman yang sama. Tujuan dari penelitian ini adalah untuk mengetahui kadar andrographolide dari tanaman sambiloto yang diambil dari 12 lokasi tumbuh dengan kondisi penanaman yang berbeda di Pulau Jawa. Daun tanaman sambiloto diekstrak dengan methanol kemudian dianalisis kandungan andrographolide menggunakan HPLC. Kadar andrographolide yang dihasilkan bervariasi berkisar antara 0,29-4,44% dengan kadar rata-rata adalah 2,19% berat kering. Kadar tertinggi didapatkan pada aksesi dari Desa Wonokaton Kabupaten Pasuruan dengan kadar andrographolide adalah 4,44% sedangkan kadar yang terendah didapatkan pada aksesi dari Desa Conggeang Kulon, Kab. Sumedang. Berdasarkan data kandungan andrographolide, diperoleh 3 aksesi sambiloto yang potensial untuk dikembangkan menjadi aksesi unggulan karena kadar andrographolidenya di atas 3%, melebihi semua yang lain.Kata kunci: Andrographis paniculata, andrographolide, senyawa aktif, HPLC, pulau Jawa

ISOLASI DAN AMPLIFIKASI DNA KELADI TIKUS (Thyponium flagelliform) UNTUK IDENTIFIKASI KERAGAMAN GENETIK Hikmatyar, Mohamad Fazri; Royani, Juwartina Ida; ., Dasumiati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Keladi tikusÃ‚Â (Typhonium flagelliforme) is one of considerable potential medicinal plants, especially as anticancer herbal medicine. In Indonesia, this plant grows throughout the island of Java, in part of Kalimantan, Sumatra and Papua. The development of Keladi tikus plants to provide raw material to meet public demand is constrained with the quality of the plants that is not standardized yet. DNA marker technique has been widely used for identification of standardization and diversity of varieties. The aims of this research were to isolate DNA from 17 accessions of Keladi tikus from various regions in Indonesia and to amplify the DNA using ISSRÃ‚Â primers. The results obtained were 17 accessions of Keladi tikus that had been isolated using the modified CTAB method. Amplifications were done by using SBLT2 and SBLT8 primers that facilitated the appearance of the polymorphism bands on the 17 accessions of Keladi tikus. Thus, SBLT2 and SBLT8 primers can be used to identify genetic variations of Keladi Tikus.Keywords: Typonium flagelliforme, Keladi tikus, ISSR, medicinal plant, amplificationÃ‚Â ABSTRAKKeladi tikusÃ‚Â (Typonium flagelliforme) merupakan salah satu tanaman obatÃ‚Â yang cukup potensial khususnya sebagai obat herbal antikanker. Tanaman ini di Indonesia tersebar di sepanjang Pulau Jawa, sebagian Kalimantan, Sumatera dan Papua. Pengembangan tanaman keladi tikus untuk memenuhi bahan baku kebutuhan masyarakat saat ini terkendala pada mutu tanaman tersebut yang belum terstandar. Teknik penanda DNA telah banyak digunakan untuk standarisasi dan identifikasi keragaman varietas. Tujuan dari penelitian ini adalah untuk mengisolasi DNA dari 17 aksesi Keladi tikus dari berbagai daerah di Indonesia dan mengamplifikasi DNA tersebut dengan primer ISSR. Hasil penelitian menunjukkan bahwa 17 aksesi keladi tikus telah dapat diisolasi dengan menggunakan metode CTAB yang dimodifikasi. Amplifikasi dilakukan dengan primer SBLT2 dan SBLT8 yang mampu memunculkan pita-pita polimorfisme pada ke 17 aksesi Keladi tikus. Primer SBLT2 dan SBLT8 dapat digunakan untuk identifikasi variasi genetic Keladi tikus.Kata Kunci: Keladi tikus, Typonium flagelliforme, ISSR, tanaman obat, amplifikasi

Cloning and Extracellular Expression of Glargine in Pichia pastoris Hardianto, Dudi; Martius, Efrida; Rostinawati, Tina; Safarrida, Anna; Royani, Juwartina Ida; Assyifa, Fahroziah; Laziba, Dihan
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.248-255

Patients with diabetes mellitus increase significantly every year. The increasing number of people with diabetes mellitus results in increased insulin requirements. There are two types of insulin used for diabetes mellitus treatment: human insulin and insulin analogues. Escherichia coli, Pichia pastoris, Saccharomyces cerevisiae, or Hansenula polymorpaha has been used to produce human insulin and insulin analogues. Pichia pastoris can produce glargine in large quantities, and the insulin protein produced will be secreted outside the cell to facilitate the purification process. The advantage of glargine has a long working time of up to 24 hours. Hence, glargine is more effective because patients with diabetes receive glargine injections only once daily. The research started with cloning the glargine gene, transforming pPICZαA-G plasmid into Pichia pastoris, and testing glargine production. 20 recombinant Pichia pastoris colonies were selected and regenerated. Eight recombinant Pichia pastoris colonies were tested for glargine production, and six colonies were detected producing glargine by electrophoresis SDS-PAGE gel stained with Coomassie blue. This study aims to produce glargine using Pichia pastoris as an expression system capable of producing glargine extracellularly, thus simplifying the purification process.

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