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All Journal JURNAL TEKNOLOGI LINGKUNGAN Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI)

SYOFI ROSMALAWATI

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Author-ID : 364473

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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Articles

PENGARUH AUKSIN DAN SITOKININ TERHADAP PERBANYAKAN MIKRO TANAMAN BINAHONG (Anredera cordifolia (Tenore) Steenis) Suparjo, .; Royani, Juwartina Ida; Rosmalawati, Syofi; Tajuddin, Teuku; Riyadi, Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 2 (2016): December 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

EFFECT OF AUXIN AND CYTOKININ ON MICROPROPAGATION OF BINAHONG (AnrederaÂ cordifoliaÂ (Tenore) Steenis)Binahong (Anredera cordifolia (Tenore) Steenis) is known as miraculous medicinal plant for its potential to cure for various kinds of diseases such as diabetes, stabilizing blood pressure and circulation, accelerating wound healing, and preventing stroke. In order to provide high quality seedlings of this medicinal plant continuously in large amount, the study on binahong micropropagation was performed. Plant growth regulators of auxins and cytokinins were applied in single or in combination so as to observe their effect on the growth of binahong explants. The results showed that 2,4-D induced callus formation in large diameter on all treatments. Nevertheless, this plant growth regulator had a negative effect on growth and development of shoot and leaves. In the combination treatments between IAA and BAP, it revealed that the higher the concentration of BAP in the media, the lower the number of leaves initiated on shoot explants. Increasing the concentration of IAA upto 1.5 ppm influenced the increasing of shoot tallness and the number of internodes. Our results can be useful for improving the binahong shoot propagation efficiency, as well as callus culture studies.Keywords: Auxin, cytokinin, callus, micropropagation, medicinal plantABSTRAKBinahong (Anredera cordifolia (Tenore) Steenis) dikenal sebagai tanaman obat ajaib karena dapat digunakan untuk pengobatan berbagai macam penyakit seperti diabetes, melancarkan peredaran dan tekanan darah, mempercepat penyembuhan luka, mencegah stroke. Dalam mendukung ketersediaan bibit tanaman obat herbal yang berkualitas secara berkelanjutan maka dilakukan kajian tentang perbanyakan bibit tanaman binahong. Zat pengatur tumbuh auksin dan sitokinin dalam bentuk tunggal maupun kombinasi diaplikasikan pada penelitian ini untuk melihat pengaruhnya terhadap berbagai eksplan binahong. Hasilnya menunjukkan bahwa 2,4-D merangsang pembentukan kalus dengan ukuran yang besar pada semua perlakuan. Namun demikian zat pengatur tumbuh ini memberikan pengaruh negatif terhadap pertumbuhan dan perkembangan tunas dan daun. Dari perlakuan kombinasi zat pengatur tumbuh IAA dan BAP, pertambahan konsentrasi BAP di dalam media menurunkan jumlah daun yang terbentuk pada eksplan pucuk binahong. Demikian pula dengan pertambahan konsentrasi IAA hingga 1,5 ppm sangat berpengaruh terhadap pertumbuhan meninggi tunas dan pertambahan jumlah ruas. Hasil dari studi ini dapat dimanfaatkan untuk studi lanjutan dalam meningkatkan efisiensi perbanyakan tunas serta kultur kalus binahong.Kata kunci: Auksin, sitokinin, kalus, perbanyakan mikro, tanaman obat herbal

Enzim Laccase dari Edible Mushroom untuk Pemutihan Pati Sagu Ramah Lingkungan Reksohadiwinoto, Budhi Santoso; Rosmalawati, Syofi; Cahyana, Purwa Tri; Hariyanto, Bambang
Jurnal Teknologi Lingkungan Vol. 18 No. 2 (2017)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Sagu pada umumnya berwarna kecoklatan dan bau kurang sedap. Kelemahan ini dapat diperbaiki dengan penambahan enzim Laccase dalam produksi pati sagu. Tujuan penelitian ini adalah menghasilkan enzm Laccase dari edible mushroom.Ã‚Â Bahan yang digunakan adalah jamur Plurotus ostreatus, Agaricus bisporus dan RhyzopusÃ‚Â oligosporus yang diperoleh dari pasar dan bahan kimia media. Metoda yang digunakan adalah melalui fermentasi cair menggunakan skala flask.Ã‚Â Hasil yang diperoleh bahwaÃ‚Â Pleurotus ostreatus pada uji agar cawan petri membentuk zona ring merah kecoklatan yang tebal menunjukan kemampuan oxidase senyawa guaicol dan syringaldazine. Uji fermentasi cair skala flaks 500 mL kultur jamur Pleurotus ostreatus, Agaricus bisporus, Rhyzopus oligosporus selama 21 hari. Hasil Laccase yang diproduksi oleh Pleurotus ostreatus mempunyai aktivitas tertinggi yaitu mencapai 774 U/L pada analisa spektrofotometer menggunakan substrat ABTS. Sementara itu, Agaricus bisporus dan Rhyzopus oligosporus mencapai puncak produksi laccase masing-masing 484 U/L dan 480 U/L.Kata kunci : Pleurotus ostreatus, Agaricus bisporus, Rhyzopus oligosporus, Laccase, bioleaching, dan kualitas pati sagu

PENGARUH AUKSIN DAN SITOKININ TERHADAP PERBANYAKAN MIKRO TANAMAN BINAHONG (Anredera cordifolia (Tenore) Steenis) Suparjo, .; Royani, Juwartina Ida; Rosmalawati, Syofi; Tajuddin, Teuku; Riyadi, Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

EFFECT OF AUXIN AND CYTOKININ ON MICROPROPAGATION OF BINAHONG (AnrederaÃ‚Â cordifoliaÃ‚Â (Tenore) Steenis)Binahong (Anredera cordifolia (Tenore) Steenis) is known as miraculous medicinal plant for its potential to cure for various kinds of diseases such as diabetes, stabilizing blood pressure and circulation, accelerating wound healing, and preventing stroke. In order to provide high quality seedlings of this medicinal plant continuously in large amount, the study on binahong micropropagation was performed. Plant growth regulators of auxins and cytokinins were applied in single or in combination so as to observe their effect on the growth of binahong explants. The results showed that 2,4-D induced callus formation in large diameter on all treatments. Nevertheless, this plant growth regulator had a negative effect on growth and development of shoot and leaves. In the combination treatments between IAA and BAP, it revealed that the higher the concentration of BAP in the media, the lower the number of leaves initiated on shoot explants. Increasing the concentration of IAA upto 1.5 ppm influenced the increasing of shoot tallness and the number of internodes. Our results can be useful for improving the binahong shoot propagation efficiency, as well as callus culture studies.Keywords: Auxin, cytokinin, callus, micropropagation, medicinal plantABSTRAKBinahong (Anredera cordifolia (Tenore) Steenis) dikenal sebagai tanaman obat ajaib karena dapat digunakan untuk pengobatan berbagai macam penyakit seperti diabetes, melancarkan peredaran dan tekanan darah, mempercepat penyembuhan luka, mencegah stroke. Dalam mendukung ketersediaan bibit tanaman obat herbal yang berkualitas secara berkelanjutan maka dilakukan kajian tentang perbanyakan bibit tanaman binahong. Zat pengatur tumbuh auksin dan sitokinin dalam bentuk tunggal maupun kombinasi diaplikasikan pada penelitian ini untuk melihat pengaruhnya terhadap berbagai eksplan binahong. Hasilnya menunjukkan bahwa 2,4-D merangsang pembentukan kalus dengan ukuran yang besar pada semua perlakuan. Namun demikian zat pengatur tumbuh ini memberikan pengaruh negatif terhadap pertumbuhan dan perkembangan tunas dan daun. Dari perlakuan kombinasi zat pengatur tumbuh IAA dan BAP, pertambahan konsentrasi BAP di dalam media menurunkan jumlah daun yang terbentuk pada eksplan pucuk binahong. Demikian pula dengan pertambahan konsentrasi IAA hingga 1,5 ppm sangat berpengaruh terhadap pertumbuhan meninggi tunas dan pertambahan jumlah ruas. Hasil dari studi ini dapat dimanfaatkan untuk studi lanjutan dalam meningkatkan efisiensi perbanyakan tunas serta kultur kalus binahong.Kata kunci: Auksin, sitokinin, kalus, perbanyakan mikro, tanaman obat herbal

Isolation and Identification of Endophytic Fungi from Srikaya Plants (Annona squamosa) Having Potential Secondary Metabolites as Anti-Breast Cancer Activity PRASETYAWAN YUNIANTO; SYOFI ROSMALAWATI; INDRA RACHMAWATI; WAHYUDI PRIYONO SUWARSO; WAHONO SUMARYONO
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Annonaceous acetogenin was extracted from Annona squamosa (Srikaya) seeds. It has cytotoxic activity against cancer cells and lower toxicity compared to other cancer drugs. Endophyte from Annonaceae is expected to have similar extracted metabolites to the host, thus increasing the economic value. This research is a preliminary study to obtain active compounds with potential as anti-cancer agents from endophytic fungi of Srikaya plants. Four endophytic fungal strains were isolated from Srikaya plants (Annona squamosa) and identified based on 28S rDNA sequence. The isolates are SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, and have similarity with Fusarium sp Vega760, Fusarium sp NRRL 22354 NRRL223, Nectria rigidiuscula, and Fusarium sp BOL35, respectively. The identified isolates were fermented in liquid media for three weeks. The liquid and mycelium were extracted using ethyl acetate. Whole extract of each fermented isolate was partitioned and evaporated to obtain ethyl acetate extract. Cytotoxicity assay of ethyl acetate extract was carried out at level 100 ppm by Methyl Thyazole Tetrazolium (MTT) viability test towards MCF-7 (breast cancer cell). The result indicated that each ethyl acetate extract could inhibit the viability of cell MCF-7 with 11.34, 99.78, 91.48, and 96.84%, for SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, respectively. Based on the results of cytotoxicity assay on MCF-7 breast cancer cells, endophytic fungi isolates SKY I.1.2, SKY II.3.2, and SKY III.3.1 are potential as sources of anti-breast cancer compounds.

Co-Authors . Suparjo, . Ahmad Riyadi Bambang Hariyanto Budhi Santoso Reksohadiwinoto, Budhi Santoso Cahyana, Purwa Tri INDRA RACHMAWATI Juwartina Ida Royani, Juwartina Ida PRASETYAWAN YUNIANTO Teuku Tajuddin Wahono Sumaryono Wahyudi Priyono Suwarso

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