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All Journal Jurnal Teknologi Laboratorium Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Pengmas Kestra (JPK) Jurnal Laboratorium Khatulistiwa Medistra Medical Journal (MMJ)
Saadah Siregar, Saadah
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Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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Journal : JURNAL FARMASIMED (JFM)

 1 
EFEKTIFITAS PENGGUNAAN PEWARNA ALTERNATIF PREPARAT PERMANEN TELUR NEMATODA USUS MENGGUNAKAN PEWARNA RHODAMIN B Siregar, Saadah; Krisdianilo, Visensius; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 2 No 1 (2019): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.612 KB) | DOI: 10.35451/jfm.v2i1.194

Abstract

Staining is one of support microscopic examination in the identification of worm eggs. Staining techniques are needed to clarify the various elements and the description of the microscopic worm eggs and distinguish it from the surrounding dirt. Hematoxylin and Eosin staining method that is widely used in the staining of histological tissue. Dyes commonly used in the examination of intestinal nematode eggs on is dye eosin. To added reference dye Rhodamine B dye used as an alternative to dye eosin. The method used is experiment by conducting concentration ratio of Rhodamine B 1%, 1.5%, 2% and 2.5% to 2% eosin control. The results based on Kruskal-Wallis Test showed the normality test is 0.357, p-value sig (0.357) > 0.05, so that the data group to be tested otherwise normal. Then performed statistical tests Kruskal WallisTtest to determine the accuracy of the file thus obtained p-value (0.407) > 0.05, meaning there is no significant difference in the concentration of the dye Rhodamine B of the entire treatment. Morphology of worm eggs clearly visible from the outside layer to the inside layer, the visual field Rhodamine B dye is clearly visible, color contrast eggs are also clearly visible. From the research conducted, it can be concluded that the dye Rhodamine B dye can be used as an alternative to dye eosin.
PERBANDINGAN AKTIVITAS ANTIBAKTERI INFUSA DAUN JERUK NIPIS (CITRUS AURANTIFOLIA) DAN DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI ESCHERICHIA COLI Siregar, Saadah; Indriani, Indriani; Vincentia Ade Rizky, Vincentia; Visensius Krisdianilo, Visensius; Anna Teresia Marbun, Romauli
Jurnal FARMASIMED (JFM) Vol 3 No 1 (2020): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i1.524

Abstract

Plant Lime (Citrus aurantifolia) and Plant Kaffir Lime (Citrus hystrix) including Rutacea family. Part of the plant lemon and lime are used as a drug other than fruit and leaves that can be used as medicine. This study aims to determine the antibacterial activity infuse lime leaves and lime leaves. Examination of the characteristics simplicia macroscopic examination. Phytochemical screening of compounds includes examining alkaloids, flavonoids, tannins and infuse saponin. Ekstrak conducted using distilled water solvent. Test of antibacterial activity against Escherichia coli bacteria carried by the agar diffusion method using a paper disc. Results obtained from macroscopic examination for lime leaves are fresh leaves leaf-shaped single smooth surface and the lower surface of the leaves are light green, the dark green upper surface. If torn, lime leaves produce coarse fibers. The leaves are small with a width of 3-5 cm. For lime leaves are fresh leaves, leaf litter pinnate compound leaves one. Child leaf blade oval to oblong, base rounded or blunt, the blunt end up pointed, 8-15 cm long, 2-6 cm wide, the upper surface colour some what shiny dark green, light green below the surface. Results of phytochemical screening simplicia powder lime leaves and lime leaves that contain a class of alkaloids, flavonoids and tannins. Antibacterial activity test results showed that there were differences between the antibacterial infusion lime leaves and lime leaves kaffir lime leaves which are more effective against the bacteria Escherichia coli compared kaffir lime leaves.
UJI EFEKTIFITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOIUM ROXB) SEBAGAI ANTIJAMUR TERHADAP PITYSPORUM OVALE siregar, Saadah; Topia, Riana
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.591

Abstract

The Pandan Wangi leaf plant is a plant that belongs to the Pandanaceae family. The metabolite content of fragrant pandan leaves (Pandanus amaryllifolius Roxb.) Alkaloids, flavonoids, saponins, tannins are known to have an antifungal effect. Pityrosporum ovale is a yeast or single-celled fungus that belongs to the genus Malassezia sp, and belongs to the Cryptococcaceae family. Pityrosporum ovale causes superficial dermatomycosis which affects the stratum corneum in the epidermis layer. This fungus is a normal flora on the scalp, but in conditions of hair with excess oil glands, this fungus can thrive. The purpose of this study was to determine the inhibition zone of fragrant pandanus leaves (Pandanus amaryllifolius Roxb.) Concentrations of 10%, 20%, 30%, and 40% of Pytirosporum ovale on Potato Dextrose Agar media by diffusion method using disc paper. The diffusion method was used to measure the diameter of the zone of inhibition against fungal growth. The positive control used was ketoconazole 2% and negative control DMSO 1%. The results of the study using the diameter of the inhibition zone at a concentration of 10% were 7.86 mm, the inhibition zone at a concentration of 20% was 8.53 mm, the inhibition zone at a concentration of 30% was 8.76 mm, and the inhibition zone at a concentration of 40% was 9, 43 mm.
Co-Authors Akmal, Dzul Anna Teresia Marbun, Romauli DALIMUNTHE, HAFIKA Indriani Indriani Krisdianilo, Vinsensius Krisdianilo, Visensius Lanny Noor, Lanny Manurung, Arnita Elisabet PUTRI, IKKE ELMALIA Rahayu, Asvia Riska Wahyuni Sri Mulyani Sutriyawan, Agung Topia, Riana Vincentia Ade Rizky Visensius Krisdianilo, Visensius Yati Supriatin, Yati