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All Journal JOIV : International Journal on Informatics Visualization Makara Journal of Health Research
Azlina Ahmad, Azlina
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Computer Science & IT

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Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous Teeth Nordin, Nur Sazwi; Mokhtar, Khairani Idah; Mustafa, Basma Ezzat; Lestari, Widya; Ichwan, Solachuddin JA Ichwan Jauhari Ichwan; Darnis, Deny Susanti; Kannan, Thirumulu Ponnuraj; Ahmad, Azlina
Makara Journal of Health Research Vol. 25, No. 1
Publisher : UI Scholars Hub

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Abstract

Background: Flaxseed promotes bone health and possibly induces bone regeneration. However, the capacity of flaxseed to induce the differentiation of stem cells into osteoblasts remains unreported. Accordingly, this study aimed to determine the effects of flaxseed extract on the osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED). Methods: SHED cultured in osteoblast induction media (OIM) were treated with 4 mg/mL flaxseed extract. RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at days 1, 3, 7, 14, and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, and DMP1). Alkaline phosphatase (ALP) activity was determined by ALP assay, and Alizarin Red-S staining was performed to evaluate calcium deposition in SHED. Results: All osteoblast markers were expressed in all samples analyzed. OSX expression was reduced in the SHED treated with flaxseed extract. In addition, the SHED treated with flaxseed extract had lower ALP activity than the control (p < 0.05). Calcium deposition was positive in the SHED cultured in OIM only. Conclusions: Flaxseed can reduce the expression of osteoblast markers, ALP activity, and calcium deposition in SHED. Thus, flaxseed potentially inhibits the osteoblast differentiation of SHED.
Evaluation of the Visual Learning Application for Mathematics using Holography Display for the topic on Shape and Space Khoo, Shiang Tyng; Badioze Zaman, Halimah; Mohamad, Ummul Hanan; Ahmad, Azlina
JOIV : International Journal on Informatics Visualization Vol 7, No 1 (2023)
Publisher : Society of Visual Informatics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30630/joiv.7.1.1679

Abstract

Mathematics is an important foundation in the life of an individual. The problems associated with learning Mathematics that are commonly encountered, are due to factors such as: abstract phenomena and concepts, low imagination, and lack of understanding of the concepts being studied. Thus, the purpose of this study is to help primary school children improve their ability to recognise 3D shapes that are abstract phenomena. This paper presents the Effectiveness Usability Evaluation of the Visual Learning Application for Mathematics using Holography Display for the topic on Shape and Space called MEL-VIS. This study was conducted on eighty (80) primary school students. The results of the study showed that learning about 3D shapes with the E-Visual MEL-VIS application prototype is more effective than traditional methods.
Co-Authors Badioze Zaman, Halimah Daniel Happy Putra Ichwan, Solachuddin JA Ichwan Jauhari Ichwan Kannan, Thirumulu Ponnuraj Khoo, Shiang Tyng LESTARI, WIDYA Mohamad, Ummul Hanan Mokhtar, Khairani Idah Mustafa, Basma Ezzat Nordin, Nur Sazwi