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All Journal Journal of Dentistry Indonesia JOIV : International Journal on Informatics Visualization Makara Journal of Health Research
Azlina Ahmad, Azlina
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Computer Science & IT Dentistry

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Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous Teeth Nordin, Nur Sazwi; Mokhtar, Khairani Idah; Mustafa, Basma Ezzat; Lestari, Widya; Ichwan, Solachuddin JA Ichwan Jauhari Ichwan; Darnis, Deny Susanti; Kannan, Thirumulu Ponnuraj; Ahmad, Azlina
Makara Journal of Health Research Vol. 25, No. 1
Publisher : UI Scholars Hub

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Abstract

Background: Flaxseed promotes bone health and possibly induces bone regeneration. However, the capacity of flaxseed to induce the differentiation of stem cells into osteoblasts remains unreported. Accordingly, this study aimed to determine the effects of flaxseed extract on the osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED). Methods: SHED cultured in osteoblast induction media (OIM) were treated with 4 mg/mL flaxseed extract. RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at days 1, 3, 7, 14, and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, and DMP1). Alkaline phosphatase (ALP) activity was determined by ALP assay, and Alizarin Red-S staining was performed to evaluate calcium deposition in SHED. Results: All osteoblast markers were expressed in all samples analyzed. OSX expression was reduced in the SHED treated with flaxseed extract. In addition, the SHED treated with flaxseed extract had lower ALP activity than the control (p < 0.05). Calcium deposition was positive in the SHED cultured in OIM only. Conclusions: Flaxseed can reduce the expression of osteoblast markers, ALP activity, and calcium deposition in SHED. Thus, flaxseed potentially inhibits the osteoblast differentiation of SHED.
Evaluation of the Visual Learning Application for Mathematics using Holography Display for the topic on Shape and Space Khoo, Shiang Tyng; Badioze Zaman, Halimah; Mohamad, Ummul Hanan; Ahmad, Azlina
JOIV : International Journal on Informatics Visualization Vol 7, No 1 (2023)
Publisher : Society of Visual Informatics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30630/joiv.7.1.1679

Abstract

Mathematics is an important foundation in the life of an individual. The problems associated with learning Mathematics that are commonly encountered, are due to factors such as: abstract phenomena and concepts, low imagination, and lack of understanding of the concepts being studied. Thus, the purpose of this study is to help primary school children improve their ability to recognise 3D shapes that are abstract phenomena. This paper presents the Effectiveness Usability Evaluation of the Visual Learning Application for Mathematics using Holography Display for the topic on Shape and Space called MEL-VIS. This study was conducted on eighty (80) primary school students. The results of the study showed that learning about 3D shapes with the E-Visual MEL-VIS application prototype is more effective than traditional methods.
Flaxseed Ethanolic Extract Modulates Β-Catenin and DKK1 Expressions During Osteoblast Differentiation of Dental Pulp-Derived Stem Cells Nordin, Nur Sazwi; Mokhtar, Khairani Idah; Abdul Qader, Omar Abdul Jabbar; Mustafa, Basma Ezzat; Lestari, Widya; Darnis, Deny Susanti; Kannan, Thirumulu Ponnuraj; Ahmad, Azlina; Arief Ichwan, Solachuddin Jauhari
Journal of Dentistry Indonesia
Publisher : UI Scholars Hub

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Background: WNT signaling regulates osteogenic differentiation of mesenchymal stem cells (MSC) through the canonical WNT pathway. β-catenin; an important molecule of WNT pathway, and DKK1; a WNT antagonist, play important roles in regulating bone formation. Although flaxseed (Linum usitatissimum) has various health benefits, including promoting bone health, our previous study showed that flaxseed ethanolic extract (FEE) reduced the osteoblast differentiation potential of stem cells from human exfoliated deciduous teeth (SHED). This study aimed to determine the effects of FEE on WNT pathway-related molecules, β-catenin and DKK1, during osteoblast differentiation of SHED. Methods: RNA at Day 7, 14, and 21 was extracted from SHED cultured in osteoblast induction media (OIM) treated with/without FEE and subjected to reverse transcriptase-PCR (RT-PCR) using the specific primers for B-Catenin and DKK1, while GAPDH acted as the internal control. Results: Treatment with FEE at 4 mg/ml significantly reduced β-catenin and DKK1 expression at day 7 (p < 0.01), increased at day 14 (p < 0.01), and later was reduced at day 21 (p < 0.01). Conclusion: Overall, FEE modulates the expressions of WNT-associated molecules: β-catenin and DKK1, during osteoblast differentiation of SHED, hence affecting the normal process of osteogenesis of SHED as demonstrated in the current analysis.
Co-Authors Abdul Qader, Omar Abdul Jabbar Arief Ichwan, Solachuddin Jauhari Badioze Zaman, Halimah Daniel Happy Putra Deny Susanti Ichwan, Solachuddin JA Ichwan Jauhari Ichwan Kannan, Thirumulu Ponnuraj Khoo, Shiang Tyng Lestari, Widya LESTARI, WIDYA Mohamad, Ummul Hanan Mokhtar, Khairani Idah Mustafa, Basma Ezzat Nordin, Nur Sazwi