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All Journal Journal of Dentistry Indonesia Makara Journal of Health Research
Mustafa, Basma Ezzat
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Dentistry

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Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous Teeth Nordin, Nur Sazwi; Mokhtar, Khairani Idah; Mustafa, Basma Ezzat; Lestari, Widya; Ichwan, Solachuddin JA Ichwan Jauhari Ichwan; Darnis, Deny Susanti; Kannan, Thirumulu Ponnuraj; Ahmad, Azlina
Makara Journal of Health Research Vol. 25, No. 1
Publisher : UI Scholars Hub

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Background: Flaxseed promotes bone health and possibly induces bone regeneration. However, the capacity of flaxseed to induce the differentiation of stem cells into osteoblasts remains unreported. Accordingly, this study aimed to determine the effects of flaxseed extract on the osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED). Methods: SHED cultured in osteoblast induction media (OIM) were treated with 4 mg/mL flaxseed extract. RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at days 1, 3, 7, 14, and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, and DMP1). Alkaline phosphatase (ALP) activity was determined by ALP assay, and Alizarin Red-S staining was performed to evaluate calcium deposition in SHED. Results: All osteoblast markers were expressed in all samples analyzed. OSX expression was reduced in the SHED treated with flaxseed extract. In addition, the SHED treated with flaxseed extract had lower ALP activity than the control (p < 0.05). Calcium deposition was positive in the SHED cultured in OIM only. Conclusions: Flaxseed can reduce the expression of osteoblast markers, ALP activity, and calcium deposition in SHED. Thus, flaxseed potentially inhibits the osteoblast differentiation of SHED.
Flaxseed Ethanolic Extract Modulates Β-Catenin and DKK1 Expressions During Osteoblast Differentiation of Dental Pulp-Derived Stem Cells Nordin, Nur Sazwi; Mokhtar, Khairani Idah; Abdul Qader, Omar Abdul Jabbar; Mustafa, Basma Ezzat; Lestari, Widya; Darnis, Deny Susanti; Kannan, Thirumulu Ponnuraj; Ahmad, Azlina; Arief Ichwan, Solachuddin Jauhari
Journal of Dentistry Indonesia
Publisher : UI Scholars Hub

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Background: WNT signaling regulates osteogenic differentiation of mesenchymal stem cells (MSC) through the canonical WNT pathway. β-catenin; an important molecule of WNT pathway, and DKK1; a WNT antagonist, play important roles in regulating bone formation. Although flaxseed (Linum usitatissimum) has various health benefits, including promoting bone health, our previous study showed that flaxseed ethanolic extract (FEE) reduced the osteoblast differentiation potential of stem cells from human exfoliated deciduous teeth (SHED). This study aimed to determine the effects of FEE on WNT pathway-related molecules, β-catenin and DKK1, during osteoblast differentiation of SHED. Methods: RNA at Day 7, 14, and 21 was extracted from SHED cultured in osteoblast induction media (OIM) treated with/without FEE and subjected to reverse transcriptase-PCR (RT-PCR) using the specific primers for B-Catenin and DKK1, while GAPDH acted as the internal control. Results: Treatment with FEE at 4 mg/ml significantly reduced β-catenin and DKK1 expression at day 7 (p < 0.01), increased at day 14 (p < 0.01), and later was reduced at day 21 (p < 0.01). Conclusion: Overall, FEE modulates the expressions of WNT-associated molecules: β-catenin and DKK1, during osteoblast differentiation of SHED, hence affecting the normal process of osteogenesis of SHED as demonstrated in the current analysis.
Co-Authors Abdul Qader, Omar Abdul Jabbar Arief Ichwan, Solachuddin Jauhari Azlina Ahmad, Azlina Daniel Happy Putra Deny Susanti Ichwan, Solachuddin JA Ichwan Jauhari Ichwan Kannan, Thirumulu Ponnuraj Lestari, Widya LESTARI, WIDYA Mokhtar, Khairani Idah Nordin, Nur Sazwi