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All Journal Jurnal Gizi dan Pangan Makara Journal of Health Research
Kannan, Thirumulu Ponnuraj
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Health Professions

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Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous Teeth Nordin, Nur Sazwi; Mokhtar, Khairani Idah; Mustafa, Basma Ezzat; Lestari, Widya; Ichwan, Solachuddin JA Ichwan Jauhari Ichwan; Darnis, Deny Susanti; Kannan, Thirumulu Ponnuraj; Ahmad, Azlina
Makara Journal of Health Research Vol. 25, No. 1
Publisher : UI Scholars Hub

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Abstract

Background: Flaxseed promotes bone health and possibly induces bone regeneration. However, the capacity of flaxseed to induce the differentiation of stem cells into osteoblasts remains unreported. Accordingly, this study aimed to determine the effects of flaxseed extract on the osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED). Methods: SHED cultured in osteoblast induction media (OIM) were treated with 4 mg/mL flaxseed extract. RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at days 1, 3, 7, 14, and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, and DMP1). Alkaline phosphatase (ALP) activity was determined by ALP assay, and Alizarin Red-S staining was performed to evaluate calcium deposition in SHED. Results: All osteoblast markers were expressed in all samples analyzed. OSX expression was reduced in the SHED treated with flaxseed extract. In addition, the SHED treated with flaxseed extract had lower ALP activity than the control (p < 0.05). Calcium deposition was positive in the SHED cultured in OIM only. Conclusions: Flaxseed can reduce the expression of osteoblast markers, ALP activity, and calcium deposition in SHED. Thus, flaxseed potentially inhibits the osteoblast differentiation of SHED.
Effectiveness of Cosmos caudatus Extract in Improving Health-Related Parameters among Older Adults with Sarcopenia: A Study Protocol Nazri, Nurul Syahidah; Vanoh, Divya; Murugaiyah, Vikneswaran; Ibrahim, Baharudin; Kannan, Thirumulu Ponnuraj; Wan Ishak, Wan Rosli; Shahar, Suzana
Jurnal Gizi dan Pangan Vol. 19 No. 2 (2024)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2024.19.2.87-89

Abstract

This is a randomized, two-arm, double-blind, placebo-controlled study that will be performed among older adults in Kelantan, Malaysia, for 12 weeks. This study aims to determine the effectiveness of a 12-week Cosmos caudatus (C. caudatus) extract supplementation on dietary intake, cognition, mental health, sleep quality, disability, gut microbiota, physical activity, frailty, metabolites, and protein synthesis pathways among older adults with sarcopenia. This study will involve 64 older adults diagnosed with sarcopenia that will be enrolled and randomly allocated with 1:1 ratio, where 32 subjects required in both intervention and placebo group. The intervention group will receive 500 mg/day of C. caudatus supplementation, while the placebo group will be given 500 mg/day of maltodextrin. Study outcomes including sarcopenia status, dementia, motoric cognitive risk, dietary pattern, anthropometric data, physical fitness, mental health, disability and sleep quality will be measured. Blood will be taken for analysis of protein synthesis pathways (using blood plasma) and untargeted metabolomics at baseline and the 12th week. Fecal sample will be collected from a subsample of 24 subjects for gut microbiota analysis. This study is one of the pioneers randomized controlled trials to evaluate C. caudatus efficacy on various health related outcomes among sarcopenic community-dwelling elderly. This study findings are hoped to have the potential to improve health awareness of ulam or fresh salad consumption, specifically C. caudatus and prove its effectiveness as a nutraceutical product for older adults with sarcopenia. The Australian New Zealand Clinical Trials Registry (ANZCTR) has this trial listed as registered with code (ACTRN12623000046606p).
Co-Authors Azlina Ahmad, Azlina Daniel Happy Putra Ibrahim, Baharudin Ichwan, Solachuddin JA Ichwan Jauhari Ichwan LESTARI, WIDYA Mokhtar, Khairani Idah Murugaiyah, Vikneswaran Mustafa, Basma Ezzat Nazri, Nurul Syahidah Nordin, Nur Sazwi Shahar, Suzana Vanoh, Divya Wan Ishak, Wan Rosli