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Pemetaan Wilayah Resiko Infeksi Virus African Swine Fever (ASF) Beserta Tindakan Pencegahan dan Pengendalian Penyebaran di Kecamatan Kota Kefamenanu, TTU Agustina Viktoria Tae; Steffanie M.C. Noach; Anastasia Kadek Dety Lestari
Jurnal Atma Inovasia Vol. 6 No. 3 (2026)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v6i3.13357

Abstract

African Swine Fever (ASF) is a highly contagious and fatal viral disease affecting pigs, and it has become a serious threat in East Nusa Tenggara Province, particularly in Timor Tengah Utara (TTU) Regency. The outbreak has resulted in high mortality rates among pigs and has directly affected the livelihoods of traditional farmers. Tubuhue Village in Kota Kefamenanu District is among the areas with the highest mortality rates due to ASF, a situation exacerbated by the high mobility of livestock and the region’s proximity to the Timor-Leste border. The main problems faced by the community include limited knowledge of ASF prevention and control, as well as the minimal implementation of biosecurity measures. The absence of easy-to-understand educational media and clear risk mapping further hinders effective mitigation efforts. These conditions threaten the sustainability of pig farming, which serves as the primary source of income for the local community. This community service program proposes solutions through risk mapping of ASF transmission and the development of an educational minibook containing practical guidelines for disease prevention and control. Continuous training and extension activities will also be conducted to enhance the community’s capacity to apply biosecurity measures consistently. This approach is expected to strengthen farmers’ preparedness and reduce the negative impacts of ASF on livestock populations and the local economy.
Detection and Levels of Escherichia coli Contamination in Beef Meatballs Sold in Kota Kefamenanu District, North Central Timor Regency Agustina Viktoria Tae; Gina Paskalia Sonia Suri; Steffanie M.C Noach
Journal of Tropical Animal Science and Technology Vol. 8 No. 2 (2026): Journal of Tropical Animal Science and Technology
Publisher : Animal Science Study Program, Faculty of Agriculture, Science and Health, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v8i2.10994

Abstract

Beef meatballs are a popular processed meat product and are highly sought after by the public due to their delicious taste, easy availability, and high nutritional value. However, this high nutritional content also makes meatballs susceptible to microbial contamination. This study aimed to detect the presence of Escherichia coli bacteria and measure the level of bacterial contamination in beef meatballs sold in Kota Kefamenanu District, North Central Timor Regency. The study used a laboratory experiment method with a purposive sampling technique on 15 beef meatball samples obtained from supermarkets and street vendors. Testing was carried out through several stages, namely initial isolation on Nutrient Agar (NA) media, cultivation on selective Eosin Methylene Blue Agar (EMBA) media, Gram staining to identify bacterial characteristics and morphology, and a Total Plate Count (TLC) test to determine the level of bacterial contamination. The results showed that all samples experienced colony growth on NA media. On EMBA media, five samples showed blackish-purple colony growth with a metallic green sheen, indicating the presence of E. coli bacteria. Gram-staining results showed pink bacteria with short, rod-shaped cells (coccobacilli) belonging to the Gram-negative bacteria group. Based on the ALT test results, four samples BK 02, BK 07, BK 09, and BK 10 had colony counts exceeding the maximum microbial contamination limit according to SNI 7388:2009, which is 5 × 101 colonies/g, and were therefore declared Not Meeting Requirements (TMS). The remaining eleven samples remained below the established threshold and were therefore safe for consumption. The conclusion of this study indicates that beef meatballs sold in Kefamenanu District are contaminated with E. coli bacteria, with most samples still meeting food safety standards. Therefore, implementing good hygiene and sanitation is essential in the processing and sales process to ensure food safety.
Giblet Organs and Digestive Performance of Native Chickens in the Pullet Phase Fed Diets Supplemented with Propionic Acid Agustina Viktoria Tae; Yuliana Naisali; Charles V. Lisnahan; Lukas Pardosi
Journal of Tropical Animal Science and Technology Vol. 8 No. 1 (2026): Journal of Tropical Animal Science and Technology
Publisher : Animal Science Study Program, Faculty of Agriculture, Science and Health, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v8i1.10601

Abstract

The purpose of this study was to examine the effect of propionic acid levels in feed on the performance of giblet organs and digestive tracts of pullet-phase Native chickens. A total of 80 Native chickens aged 12 weeks with an average weight of 400 g were used in this study. The research design applied was a Completely Randomized Design (CRD). Chickens were randomly divided into four treatments with four replications, where each replication consisted of four Native chickens. The treatments given were T0: control feed without propionic acid; T1: control feed + 0.25% propionic acid; T2: control feed + 0.50% propionic acid; T3: control feed + 0.75% propionic acid. Data analysis was conducted using ANOVA, followed by the Duncan Test to determine differences between treatments. The results showed that live weight, giblet weight, pancreas weight, intestine weight, intestine length, proventriculus weight, were highest in the T3 control feed + 0.75% propionic acid treatment, respectively, at 1622.36±38.24 g/head; 59.23±0.39 g/head; 3.54±0.04 g/head; 27.24±0.57 g/head; 27.24±0.57 g/head; 129.33±0.68 cm/head; 6.04±0.18 g/head. The highest giblet percentage and proventriculus percentage were in the T0 control feed treatment without propionic acid at 3.74±0.12%/head and 0.38±0.01%/head, respectively. The highest percentage of pancreas weight was in the T2 treatment of control feed + 0.50% propionic acid and the T3 treatment of control feed + 0.75% propionic acid, which was 0.22 ± 0.01%/head. The highest percentage of intestine weight was obtained in the T2 treatment (control feed + 0.50% propionic acid) with a value of 1.71 ± 0.03%/head. Analysis of variance showed that the treatment significantly affected the live weight, giblet organ weight, pancreas weight, percentage of pancreas weight, small intestine weight, intestine length, and proventriculus weight of pullet phase Native chickens (P <0.05). It can be concluded that the addition of 0.75% propionic acid to the control feed had a positive effect on the performance of the giblet organ and digestive tract of Native chickens.