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All Journal Buletin Peternakan JURNAL KIMIA SAINS DAN APLIKASI Jurnal Pendidikan Biologi Undiksha JURNAL PENGABDIAN KEPADA MASYARAKAT Pro-Life BERITA BIOLOGI Biodidaktika : Jurnal Biologi dan Pembelajarannya Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat BIOEDUSCIENCE Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA JMM (Jurnal Masyarakat Mandiri) MATAPPA: Jurnal Pengabdian Kepada Masyarakat Jurnal Saintek Lahan Kering JPBIO (Jurnal Pendidikan Biologi) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Community Development Journal: Jurnal Pengabdian Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Bakti Cendana: Jurnal Pengabdian Masyarakat Journal of Tropical Animal Science and Technology Jurnal Pengabdian UNDIKMA Jurnal Biosense Bioscientist : Jurnal Ilmiah Biologi Prosiding Konferensi Nasional PKM-CSR Society: Jurnal Pengabdian Masyarakat Dharma Pengabdian Perguruan Tinggi (DEPATI) JURNAL BIOLOGI PAPUA BIO-CONS: Jurnal Biologi dan Konservasi Quagga: Jurnal Pendidikan dan Biologi Journal Science of Biodiversity Journal Science of Biodiversity BIOGENIC: Jurnal Ilmiah Biologi Journal of Chemical Science and Application Berita Biologi

Lukas Pardosi

Prodi Biologi, Universitas Timor, Indonesia

Author-ID : 3208446

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Isolasi dan identifikasi kapang Rhizopus pada tempe kacang merah (Phaseulus vulgaris L) Moensaku, Elmalinda; Sine, Yuni; Pardosi, Lukas
Jurnal Pendidikan Biologi undiksha Vol 8, No 2 (2021)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Tempe merupakan produk Indonesia yang difermentasi oleh Rhizopus oligosporus. Kacang merah (Phaseolus vulgaris L) berpotensi untuk diolah menjadi tempe. Tujuan dari penelitian ini mengetahui bentuk koloni kapang Rhizopus pada tempe kacang merah, mengetahui karakter morfologi baik secara makroskopis dan mikroskopis tempe kacang merah, mengetahui potensi kapang Rhizopus dalam menghambat patogen. Penelitian ini dilaksanakan pada bulan Januari-Maret 2021 di Laboratorium Pertanian Universitas Timor. Tempe kacang merah diisolasi dan diidentifikasi dengan menggunakan metode pengenceran berseri dan spread plate. Hasil penelitian menunjukan bahwa terdapat 3 isolat yang digunakan untuk proses identifikasi. Pengamatan karakter makroskopis pengenceran tempe kacang merah memiliki warna koloni putih, bentuk koloni bulat, tepi koloni rata, elevasi raised (timbul), ukuran koloni kecil pada masing-masing isolat. Tempe kacang merah sebagai substrat pertumbuhan Rhizopus sp. Kapang tempe didominasi oleh kapang berwarna putih keabu-abuan yang diidentifikasi mirip dengan Rhizopus oligosporus. Rhizopus sp bersifat antagonis terhadap bakteri uji, yaitu menghasilkan zona hambat sebesar 20 terhadap Escherichia coli, 27 terhadap Staphylococcus aureus. Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwa kapang pada tempe kacang merah difermentasi oleh Rhizopus oligosporus dan Rhizopus bersifat antagonis terhadap bakteri patogen. Hasil penelitian ini menguatkan manfaat tempe sebagai pangan fungsional.

Aktivitas Mikrobiologis Endofit dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Jendri Mamangkey; Lukas Pardosi; Riska Septia Wahyuningtyas
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Endophytic bacteria and fungi inhibite the tisues of living plant. Both of them are significant components of plant microecosystems. Current research was performed to explain several microbiological activities (production of extracellular enzymes, antioxidants, and antibacterials) of endophytic bacteria and fungi from binahong leaves extract (Anredera cordifolia (Ten.) Steenis). In this case, the research was conducted by applying a review of several literature reviews published online on Google Scholar and several journals listed on the ScienceDirect site. Based on the results obtained from the research reports from several scientific journal sources, there are four types of extracellular enzymes produced by the endophytic bacteria of the Anredera cordifolia plant, including proteinase, amylase, esterase, and cellulase. In addition, the endophytic bacteria of Pseudomonas aeruginosa from the leaves extract of Anredera cordifolia has antioxidant activity, which is volatile compounds including cis-9-Octadecenoic acid, 2-dodecenoic acid, and 9 octadecenamide produced by endophytic bacteria of Pseudomonas sp. Furthermore, the endophytic bacteria isolated from Anredera cordifolia leaves extract inhibits the growth of E. coli ATCC 35216 and S. aureus ATCC 29523. Meanwhile, the endophytic fungi from Anredera cordifolia leaves extract inhibite the growth of E.coli and S. aureus bacteria. Therefore, understanding the relationship between bacteria and endophytic fungi from the leaves extract of binahong (Anredera cordifolia (Ten.) Steenis) is essential. This knowledge can be utilized and applied well for the future production of higher quality and more medicinal plants from binahong plant (Anredera cordifolia (Ten.) Steenis).

Isolasi dan identifikasi kapang Rhizopus pada tempe kacang merah (Phaseulus vulgaris L) Elmalinda Moensaku; Yuni Sine; Lukas Pardosi
Jurnal Pendidikan Biologi undiksha Vol. 8 No. 2 (2021)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar

PELATIHAN OPTIMALISASI MIKROSKOP KONVENSIONAL BAGI GURU BIOLOGI SEKOLAH MENENGAH ATAS Gede Arya Wiguna; Welsiliana Welsiliana; Florian Mayesti prima R Makin; Ite Morina yostianti Tnunay; Lukas Pardosi; Andi Faesal
JMM (Jurnal Masyarakat Mandiri) Vol 5, No 6 (2021): Desember
Publisher : Universitas Muhammadiyah Mataram

Abstrak: Biologi merupakan pelajaran IPA yang mempelajari kehidupan organisme, termasuk struktur, fungsi dan pertumbuhannya. Pembelajaran biologi perlu menambahkan keterampilan menggunakan alat peraga atau praktikum seperti pengamatan bentuk sel, ukuran sel, serta pergerakan mikroorganisme. Oleh karena itu perlu adanya sarana berupa mikroskop. Keterbatasan ketersedian mikroskop yang dimiliki sekolah mengakibatkan guru kurang optimal dalam melakukan penelitian dan pengajaran. Oleh karena itu perlu adanya optimalisasi kinerja mikroskop dengan cara mengubah mikroskop konvensional menjadi digital. Berdasarkan permasalahan tersebut, maka diadakan pelatihan Optimalisasi Mikroskop konvensional menjadi digital bagi guru biologi. Pelatihan mulai dengan pengenalan fungsi masing-masing komponen alat tambahan seperti kamera, lampu, dan perangkat lunak penangkap gambar. Selanjutnya dilakukan pelatihan instalasi dan mengoperasikan perangkat lunak penangkap gambar serta pengamatan menggunakan mikroskop digital. Pelatihan terakhir yaitu analisis gambar yang diperoleh dari mikroskop digital menggunakan perangkat lunak Image J. Hasil evaluasi menunjukkan bahwa 4 guru biologi sudah mampu mencapai level 3 yang artinya guru menguasai penggunaan mikroskop digital, penggunaan perangkat lunak serta melakukan analisis gambar menggunakan Image J. Tingkat pemahaman guru menggunakan mikroskop digital mencapai 90%. Abstract: Biology is a science lesson that studies the life of organisms, including their structure, function and growth. Biology learning needs to add skills using teaching aids or practical such as observing cell shape, cell size, and the movement of microorganisms. Therefore, it is necessary to have a tool in the form of a microscope. The limited availability of microscopes owned by schools causes teachers to be less than optimal in conducting research and teaching. Therefore, it is necessary to optimize the performance of the microscope by converting conventional microscopes to digital ones. Based on these problems, a training on Optimization of Conventional Microscopes to be Digital was held for biology teachers. The training begins with an introduction to the function of each component of the auxiliary equipment such as cameras, lights, and image capture software. Furthermore, training on the installation and operation of image capture software and observations using a digital microscope was carried out. The last training was analysis of images obtained from digital microscopes using Image J software. The evaluation results show that 4 biology teachers have been able to reach level 3, which means that teachers able to use of digital microscopes, use software and image analysis using Image J. The level of understanding of teachers using digital microscopes reaches 90%

Eksplorasi Bakteri Endofit Dari Jahe Merah (Zingiber officinalevar. Rubrum) Asal Pulau Timor Gergonius Fallo; Yuni Sine; Lukas Pardosi
Saintek Lahan Kering Vol 2 No 2 (2019): JSLK Desember 2019
Publisher : Fakultas Pertanian, Universitas Timor

Ginger (Zingiber officinale) is one of the plants of the zingiberaceae family that grows evenly on the island of Timor. The condition of the island of Timor with a long dry season (± 8 months) resulted in the condition of the plants being in a state of drought stress. The purpose of this study was to obtain endophytic bacteria from ginger plants and to identify macroscopically, and test the endophytic bacterial antagonists as producing antibiotics. Samples were obtained from North Central Timor District (TTU), especially from Naibenu District, Kefamenanu City District, and West Miomaffo District. Bacterial isolation method used is the method of spreading and planting on petri dishes using Nutrient Agar (NA) media. While the test of antibiotic-producing activity is seen from the formation of inhibitory zones around the colony. The results of isolation of endophytic bacteria in ginger plants obtained 14 isolates that have the potential to produce antibiotics. Antagonist test results showed that endophytic bacterial isolates were able to inhibit the growth of Staphylococus aureus bacteria with the highest inhibitory zone diameter, namely from JMKBD3 isolates of 2.66 mm. Morphological identification of JMKBD3 isolate colonies is known that irregular colony shape, undulate edge, flat elevation, and white colony color.

EKSPLORASI JAMUR MAKROSKOPIS DI HUTAN OELUAN KABUPATEN TIMOR TENGAH UTARA Lukas Pardosi; Florian Mayesty Prima Makin; I Gede Arya Wiguna
Saintek Lahan Kering Vol 3 No 1 (2020): JSLK JUNI 2020
Publisher : Fakultas Pertanian, Universitas Timor

Research on the diversity of macroscopic fungi in the Oeluan Forest area of North Central Timor district has never been reported. This research was conducted in January 2020 with a geographical location of 9° 2¢ 48 "LS-9° 37¢ 36" LS124° 4¢ 2 "BT-124°46¢ 0" BT, this research was carried out on four different lines. The length of the observation path is 100 m. The method used in each observation lane in each method is the roaming method and purposive sampling, based on the presence of macroscopic fungi that are considered to represent the area, after that it is continued by recording the number of individuals, collections and documentation. Observation and collection of species is carried out exploratively. From the results of the study found 9 species of macroscopic fungi namely, Ganoderma aplatum, Earliella scabrosa, Polyporus tenuiculus, Polyporus squamosus, Mikroporus xanthopus, Favolus grammocephalus, Pycnoporus cinnabarinus, Ganoderma lucidium, Laccaria vinaceoavellaneae

Physical properties of local upland rice kupang east nusa tenggara, indonesia Dicky Frengky Hanas; Ite Morina Y Tnunay; Welsiliana Welsiliana; Florian Mayesti Prima R Makin; Lukas Pardosi; I Gede Arya Wiguna; Lidwina Felisima Tae
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 1 (2022): April 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i1.1300

Local upland rice (Oryza sativa L.) is a type of cereal crop that has the potential for dry land areas. Farmers in Kupang plant upland rice/local rice that has been consumed and used for generations and is considered by the surrounding community as part of their ancestral heritage. The materials used in this research were local upland rice varieties as a result of exploration. The varieties of six local upland rice of Kupang studied in this research had different rice characters in terms of its weight, volume, density and yield.'Aen Molo' has the highest weight per grain and per 1000 grains, namely 0.024 g and 24.95 g, also the highest volume of 1000 grains of rice is 30 ml, while 'Aen Lekes' has the lowest weight per grain and per 1000 grains, namely 0.020 g and 19.66 g and also the lowest volume of 1000 grains, namely 23 ml. The Iodine test results showed that the local rice of Kupang was classified in the regular or non-glutinous rice group. Meanwhile, Alkali test results show that the six samples of local upland rice have a high gelatinization temperature which indicates that local upland rice in Kupang Regency has high amylose content.

PELATIHAN PEMBUATAN NATA DE COCO BAGI MASYARAKAT KELOMPOK TANI SEHATI DESA TUBLOPO Yuni Sine; Lukas Pardosi; Dicky Frengky Hanas; Hermina Manlea; Mardit N. Nalle
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol 2 No 4 (2021)
Publisher : Universitas Majalengka

Pengabdian nata de coco dilakukan oleh tim pengabdi yaitu dosen dari Universitas Timor, kepada masyarakat Desa Tublopo, Dusun Banopo, kelompok Tani Sehati. Dusun Banopo memiliki banyak sumber daya yang dpat dikembangkan, salah satunya adalah kelapa. akan tetapi pengolahannya belum maksimal sehingga perlu diadakan pelatihan pengolahan pangan dengan memanfaatkan sumber daya lokal yang ada guna meningkatkan nilai jual produk serta menghasilkan produk dengan inovasi baru yang mempunyai kualitas lebih baik. Air kelapa mempunyai potensi yang baik untuk di buat minuman fermentasi karena kandungan zat gizinya yang kaya dan relatif lengkap, sehingga sesuai untuk pertumbuhan mikroorganisme. Metode yang digunakan dalam kegiatan pengabdian ini berupa pelatihan dan praktek pembuatan Nata de coco kepada Kelompok Tani Sehati. Observasi awal menunjukkan masyarakat belum mengetahui pemanfaatan air kelapa sebagai substrat nata de coco, dengan penjelasan sederhana dan contoh produk nata de coco pada minuman membuat anggota kelompok tani mengetahui bahwa nata de coco sudah sering di konsumsi. Pada saat praktek tim pengabdi melakukan contoh pertama sedangkan selanjutnya di kerjakan oleh anggota kelompok tani. Nata de coco hasil fermentasi masih sedikit hal ini dapat disebabkan oleh berbagai hal yaitu, lingkungan (suhu dan kelembapan), kualitas substrat dan kualitas starter. Anggota Kelompok Tani Sehati telah memiliki ketrampilan pembuatan nata de coco dan diharapkan untuk dapat melatih ketrampilan tersebut sehingga menghasilkan produk nata de coco yang memiliki merk dagang sendiri.

Pencegahan Penularan COVID-19 Melalui Penguatan Perilaku Siswa dan Guru SDK Seoam 1 Eban Kecamatan Miomaffo Barat Grandianus Seda Mada; Dicky Frengky Hanas; Lukas Pardosi; Welsiliana Welsiliana; Didi Prasetyo Benu; Marselina Theresia Djue Tea; Dira Asri Pramita; Desta Gloria Siahaan
Bakti Cendana Vol 4 No 2 (2021): Bakti Cendana: Jurnal Pengabdian Masyarakat
Publisher : LPPM Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/bc.4.2.2021.1-7

Salah satu alasan sekolah masih belum dibuka secara penuh hingga kini adalah potensi terjadinya penularan melalui siswa-siswa yang berkumpul dalam waktu lama di ruang tertutup. Beberapa penelitian menunjukan bahwa menutup sekolah akan memperlambat perkembangan infeksi. Namun, langkah-langkah yang lebih luas seperti social distancing, terbukti memiliki efek pengendalian yang lebih besar. Risiko dari pembukaan sekolah akan bergantung pada seberapa baik sekolah mengendalikan penularan, misalnya dengan penggunaan masker dan membatasi okupansi. Oleh karena itu, perlu dilakukan kembali penguatan perilaku untuk mencegah penularan penyakit Covid-19 kepada siswa dan guru. Bentuk Kegiatan Pengabdian yang dilakukan adalah pembagian masker dan edukasi mengenai dampak penyakit Covid-19 kepada siswa dan guru SDK Seoam 1 Eban, Kefamenanu. Hasil dari kegiatan ini adalah para siswa dan guru dapat melakukan protokol kesehatan secara sadar dan benar yaitu melalui 3M: memakai masker, menjaga jarak dan menghindari kerumunan, serta mencuci tangan pakai sabun. Kata Kunci: Covid-19, Protokol Kesehatan, Guru dan Siswa

CHARACTERIZATION AND IDENTIFICATION OF SM4 BACTERIAL ISOLATE FROM Stylissa massa SPONSE AS PRODUCING ANTIMICROBIAL COMPOUNDS AGAINST PATHOGENIC BACTERIA Lukas Pardosi
BERITA BIOLOGI Vol 21, No 1 (2022)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4187

East Nusa Tenggara (NTT) is one of the archipelagic provinces in Indonesia with a sea area of 200,000 km² and contains potential marine resources including the Stylissa massa sponge. Bacteria have been known to associate with sponges and have the ability to produce antimicrobial compounds. This study aims to characterize, identify the 16S rRNA gene, and test the bioactive compounds of SM4 bacterial isolates from the Stylissa massa sponge. The bacterial isolates of SM4 were previously rejuvenated on NA medium, then characterized by macroscopic morphology, and biochemistry by citrate test and TSIA test. The culture was extracted using petroleum ether solvent and the antibacterial activity was tested against E. coli and S. aureus by the well method. The isolates were then identified based on the 16S rRNA gene by PCR method. The results showed that, SM4 isolates had small colony size, yellow color, round colony shape, flat elevation, flat margins, while biochemically they had positive results with Citrate and TSIA tests. The antagonist test of the SM4 bacterial isolate was found to have the ability to inhibit the growth of S. aureus and E. coli, while the antibacterial activity test of the bioactive compound bacterial isolate SM4 was known to have the ability to inhibit the growth of S .aureus with an inhibition zone of 1.99 mm and E. colli 1.56 mm. The results of the molecular identification of the bacterial isolate SM4 had 99% similarity with the Bacillus cereus strain Nr_074540.1

Co-Authors Agustina Yovita Boluk Alfred Nubatonis Anastasia Buak Anggelina Mokos Anjelina M. da Cruz Arkianti, Maria Maxmilla Yoche Azor Yulianus Tefa Banusu, Maria Sarliana Batu, Matius S Benu, Didi Prasetyo Bira, Gerson Frans Bria, Deseriana Charles Venirius Lisnahan Dira Asri Pramita Eduardus Yosef Neonbeni Elmalinda Moensaku Emanuel M. Y. Hano’e Emanuel M. Y. Hano’e Emilia Juliyanti Bria Faesal, Andi Fallo, Gergonius Florian Mayesti Prima Remba Makin Fobia, Kaprisius G. Fransiskus Yulius Dhewa Kadju Hanas, Dicky Frengky Hano’e, Emanuel M. Y. Hermina Manlea Hermina Manlea I Gede Arya Wiguna Jefry Presson Jefry Presson jefry Presson Jendri Mamangkey Josua Sahala Jusanty Antoneta Pakh Kamaluddin Kamaluddin Kamaluddin Kamaluddin Kia, Kristoforus W Kia, Kristoforus Wilson Kia, Kristoforus. W. Kolo, Maria Magdalena Korbafo, Elisabeth Kristoforus Wilson Kia Lidya, Christie Liem, Noviana Animra Lisnahan, Charles V. Lusiana Yeni Akoit Mada, Grandianus Seda Manalu, Adelya I Mardit N Nalle Maria Ernalinda Seran Matius Stefanus Batu Meta Permata Putri Metriana Leon Moensaku, Elmalinda Nahak, Oktovianus Rafael Naimuni, Fransiskus Xaverius Natalia Desy Djata Ndua Natalia Hoar Neonbeni, Eduardus Yosef Nugraha K. F. Dethan Pakh, Jusanty Antoneta Pramita, Dira Asri Pramusita, Santa Maya Presson, Jefry Respati Tri Swasono Riska Septia Wahyuningtyas Risna Erni Yati Adu Sabirin Matsjeh Sefrinus Maria Dolfi Kolo Seuk, Mariana Septiani Siahaan, Desta Gloria Sine, Yuni Sio Stefanus, Sio Sipayung, Boanerges Putra SITUMORANG, KOMILIE Tae, Lidwina Felisima Tahulending, Peggy Tea, Marselina Theresia Djue Tefa, Anna tnunay, ite morina yostianti Tode, Sonya Yuniarsi Welsiliana Welsiliana Widhya Faustina Wiguna, Gede Arya Wilda Lumban Tobing Yakobus Pffeferius Edvend Saba Agu Yohana Fatmawati Usfinit Yos Bobi A. Olla Yuliana Kolo Yuni Sine Yuni Sine Zulfah Az Zahra

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