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All Journal Annales Bogorienses
Miranti Nurindah Sari, Miranti Nurindah
Pusat Penelitian Bioteknologi-LIPI, Jl. Raya Bogor KM.46 Cibinong 16911

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Isolation and Characterization of Bacterial Carbonate Precipitation for Biogrouting Suyanto, Eko; Sari, Miranti Nurindah; Ratnakomala, Shanti; Fahrurrozi, Fahrurrozi; Gusmawati, Niken F.
Annales Bogorienses Vol. 25 No. 2 (2021): Annales Bogorienses
Publisher : BRIN

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Abstract

Biogrouting is a process that transforms soil or sand into calcarenite or sandstone by bacterial calcium carbonate precipitation . The mechanisms of this process are that the bacteria hydrolyze urea catalyzed by urease, and with the existence of dissolved Ca2+, a solid crystalline calcite or calcium carbonate (CaCO3) will be produced. The main advantage of biogrout is that soil or sand can be strengthened without interfering with the hydraulics of the treated soil or sand and without excavation or replacement. In this study, the isolation and identification of bacterial calcium carbonate precipitation , and characterization of urease produced by bacteria were conducted. In the isolation method, the enrichment method using urea as abacterial carbon source was carried out. The formation of crystalline calcite was observed by a light microscope. The urease enzyme activity was determined by the 3, 5-Dinitrosalicylic acid (DNS) method. The molecular identification of isolates was analyzed by the determination of 16S rRNA gene sequencing. As a result, 19 calcium carbonate precipitation have been isolated from soils, sands, water, and rocks collected from several areas in Indonesia. They showed calcite formation in a medium with urea. Molecular identification of isolates with high urease enzyme activity revealed that the isolates belong to the group Bacilli. The highest enzyme activity produced by ID10-U004 is 374.94 U/mL. The preliminary biogrouting experiment was also conducted using isolated strain, and the reaction was able to make the sand solid and stiff.
Antimicrobial Activities of Actinobacteria Isolated from Marine and Terrestrial Samples in Lombok Island, Indonesia Ratnakomala, Shanti; Krause, Janina; Sari, Miranti Nurindah; Kusumawardhani, Dinihari Indah; Kusmiati, Mia; Lisdiyanti, Puspita
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.28-36

Abstract

During our study on exploring actinobacteria from Indonesian sources, we selected 59 strains of actinobacteria isolated with five different isolation methods from Lombok Island Indonesia. The aim of this research was to isolate and characterize those actinobacteria, and to screen the microbial activities against 5 bacterial tested. Six soil samples from Lombok Island were processed. Totally, 59 isolated were selected. Of these strains, 31 strains showed antibiotic activity. Seventeen strains from 59 strains have been identified by 16S rRNA gene sequencing, and most dominant of the strains belongs to the genus Streptomyces. Three of them were chosen for further experiments due to their broad activity against four of five test strains. From two strains, at least one bioactive substance could be extracted. As only few bioactive substances could be extracted with ethyl acetate from liquid cultures more experiments should be performed on this subject. More solvent extraction should be tested as well as other production media. In conclusion, present study did now reveal bioactive actinobacteria were isolated from soil samples in Lombok Island. Antimicrobial compound has also been detected which is active against a broad range of pathogens. These findings may have an importance to produce potentially new antibiotic substances from actinobacteria indigenous Indonesia.
Co-Authors Eko Suyanto Fahrurrozi Fahrurrozi Gusmawati, Niken F. Krause, Janina Kusumawardhani, Dinihari Indah Mia Kusmiati PUSPITA LISDIYANTI SHANTI RATNAKOMALA