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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Jurnal Riset dan Kajian Pendidikan Fisika Biotropika JURNAL ILMIAH PENDIDIKAN FISIKA AL BIRUNI Bioeksperimen: Jurnal Penelitian Biologi JIPF (Jurnal Ilmu Pendidikan Fisika) Jurnal Riset Biologi dan Aplikasinya JSMARTech : Journal of Smart Bioprospecting and Technology Ruang Pengabdian : Jurnal Pengabdian Kepada Masyarakat Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Engagement: Jurnal Pengabdian Kepada Masyarakat Jurnal Pendidikan MIPA Annales Bogorienses
Eko Suyanto
1) Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Indonesia 2) Research Center Of Smart Molecule Of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa Suyanto, Eko; Soetarto, Endang Sutariningsih; Cahyanto, Muhammad Nur
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: Maret 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lipase memiliki manfaat penting di bidang industri. Tujuan penelitian ini adalah untuk mendapatkan kapang lipolitik yang mampu tumbuh dan menghasilkan aktivitas lipase tinggi pada limbah ampas kelapa menggunakan metode solid state fermentation. Isolat kapang uji dipurifikasi kemudian dilakukan skrining dan seleksi kapang lipolitik dan dilanjutkan dengan produksi lipase menggunakan substrat ampas kelapa yang sebelumnya diukur kandungan biokimia. Hasil menunjukkan bahwa 8 isolat kapang lipolitik mampu tumbuh baik pada substrat ampas kelapa yang ditunjukkan dengan adanya sporulasi dan perubahan pH medium selama reaksi. Diantara kapang lipolitik tersebut, isolat kapang KLC-333 diketahui menghasilkan aktivitas hidrolisis lipase terbesar yaitu 13,33 U/ml dan volume produksi 46 ml. Biosintesis dan peningkatan produksi lipase dipengaruhi oleh kandungan nutrien di dalam substrat ampas kelapa.
Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa Suyanto, Eko; Soetarto, Endang Sutariningsih; Cahyanto, Muhammad Nur
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: Maret 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i1.311

Abstract

Lipase memiliki manfaat penting di bidang industri. Tujuan penelitian ini adalah untuk mendapatkan kapang lipolitik yang mampu tumbuh dan menghasilkan aktivitas lipase tinggi pada limbah ampas kelapa menggunakan metode solid state fermentation. Isolat kapang uji dipurifikasi kemudian dilakukan skrining dan seleksi kapang lipolitik dan dilanjutkan dengan produksi lipase menggunakan substrat ampas kelapa yang sebelumnya diukur kandungan biokimia. Hasil menunjukkan bahwa 8 isolat kapang lipolitik mampu tumbuh baik pada substrat ampas kelapa yang ditunjukkan dengan adanya sporulasi dan perubahan pH medium selama reaksi. Diantara kapang lipolitik tersebut, isolat kapang KLC-333 diketahui menghasilkan aktivitas hidrolisis lipase terbesar yaitu 13,33 U/ml dan volume produksi 46 ml. Biosintesis dan peningkatan produksi lipase dipengaruhi oleh kandungan nutrien di dalam substrat ampas kelapa.
Predominant Bacterial Diversity in Rheumatoid Arthritis Rat After Treated with Caprine CSN1S2 Protein Suyanto, Eko; Fatchiyah, Fatchiyah
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.01

Abstract

Introduction: Rheumatoid arthritis (RA) is an autoimmune and systemic inflammatory disease influenced by microbial abnormalities (dysbiosis) in the intestinal that affect changes in metabolism and immune system disorders. This study aimed to investigate the predominant intestinal microbiota in complete Freund’s Adjuvant-induced rheumatoid arthritis rats after treated with caprine milk CSN1S2 protein through fecal analysis based on PCR-DGGE and to construct the phylogenetic tree of bacteria as the evolutionary relationship. Method: The experimental animals were divided into 6 groups with 2 types of rat model, namely control rat (C group, CM group, and CY group) and RA rat (RA group, RAM group, and RAY group). Predominant cultivable microbiota was obtained by direct culture and analyzed using PCR-DGGE with some specific primers. The DNA sequences were analyzed and aligned using bioinformatics software to construct the phylogenetic tree. Results: We found that Lactobacillus group significantly increased in the control rat model and the predominant intestinal bacteria in RA rats were Enterococcus group (Enterococcus faecium and Enterococcus faecalis). Conclusions: The caprine milk CSN1S2 protein influences the composition of microbiota in RA rats with the emergence of predominant bacteria that are considered species of the Bacillus group, closely related to Bacillus coagulans, which can be promoted the growth of B. coagulans to suppress pathogenic bacteria in the development of RA disease.
Pengaruh Protein CSN1S2 dari Susu dan Yogurt Kambing Peranakan Ethawah Terhadap Komposisi Mikrobiota pada Feses Tikus RA-CFA Adhya Dava Aligarh Yahya; Eko Suyanto; Fatchiyah Fatchiyah
Biotropika: Journal of Tropical Biology Vol 8, No 2 (2020)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2020.008.02.06

Abstract

Rheumatoid arthritis (RA) merupakan salah satu penyakit autoimun yang menyebabkan inflamasi pada jaringan sinovial. Prevalensi RA semakin meningkat setiap tahunnya dan berpotensi menghambat produktivitas. Komposisi mikrobiota merupakan salah satu faktor lingkungan yang mempengaruhi perkembangan penyakit RA. Tujuan penelitian ini adalah untuk mengamati efek pemberian protein CSN1S2 susu dan yogurt peranakan Ethawah terhadap komposisi mikrobiota pada usus tikus rheumatoid arthritis yang diinduksi Complete Freund’s Adjuvant (CFA). Tikus dibagi menjadi dua model yakni kontrol dan RA yang masing – masing diberi perlakukan protein CSN1S2 susu dan yogurt. Feses yang diperoleh lalu dianalisis total koloni bakteri, isolasi bakteri, karakterisasi, perhitungan indeks Simpson, isolasi DNA bakteri dan amplifikasi gen 16S sRNA. Hasil menunjukkan bahwa pemberian protein CSN1S2 pada model hewan mempengaruhi komposisi bakteri dengan peningkatan jumlah koloni bakteri pada kelompok RAM dan RAY. Pada perlakuan kontrol dan RA didominasi oleh kelompok bakteri Lactobacillus berdasarkan analisis gen 16S rRNA. Kelompok Lactobacillus lebih banyak ditemukan pada perlakuan kontrol dibandingkan RA. Lactobacillus berperan dalam mempengaruhi perkembangan penyakit RA, serta mampu menghambat bakteri patogen dari golongan Clostridium dan Enterococcus, ini bergantung pada tingkat spesies bakteri tersebut.
Pengaruh Bioenkapsulasi Artemia salina Terhadap Tingkat Kelangsungan Hidup Benih Ikan Nila (Oreochromis niloticus) Eko Suyanto; Yasir Saifur Rahman; Murwantoko Murwantoko
Biotropika: Journal of Tropical Biology Vol 7, No 2 (2019)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2019.007.02.5

Abstract

Permintaan ikan nila (Oreochromis niloticus) semakin meningkat dari tahun ke tahun namun terkendala dalam ketersediaan benih karena tingginya resiko serangan penyakit streptococcosis yang menurunkan kelangsungan hidup (sintasan) benih. Tujuan penelitian ini adalah adalah untuk mengetahui pengaruh pemberian pakan bioenkapsulasi Artemia salina dengan Spirulina platensis terhadap sintasan benih ikan nila yang telah diinjeksi bakteri Streptococcus sp. dalam upaya mencegah penyakit streptococcosis. Bioenkapsulasi dilakukan dengan cara kultur A. salina diberi pakan tepung S. platensis ukuran 37 mikron selama 5 jam lalu dipanen dan disimpan pada suhu 4oC. Bioenkapsulan dianalisis proksimat meliputi kadar air, kadar protein total, kadar lemak, kadar abu, kadar serat kasar dan kadar karbohidrat sedangkan analisis kadar asam lemak dianalisis menggunakan Gas chromatography. Perlakuan pakan benih ikan nila yaitu bioenkapsulan dan pellet ikan dengan perbandingan variasi 25%:75% (BASP1), 50%:50% (BASP2), 75%:25% (BASP3), 100%:0% (BASP4) dan 0%:100% (K) lalu diuji tantang dengan bakteri Streptococcus sp konsentrasi 106 CFU/mL secara rendaman. Pakan bioenkapsulasi A. salina dengan S. platensis memberikan pengaruh terhadap tingkat kelangsungan hidup benih ikan nila. Peningkatan kelangsungan hidup terbaik diperoleh pada hari ke-7 sebesar 26,7%. Pemberian pakan bioenkapsulan dengan konsentrasi 50-75% cukup efektif diberikan hingga hari ke-14 untuk meningkatkan sintasan benih ikan nila dalam usaha pencegahan penyakit streptococcosis. Semakin tinggi konsentrasi pakan bioenkapsulan akan meningkatkan resiko kematian benih ikan nila. 
Oral Administration of The Hypercholesterol Rat Feed Formula to Making The Animal Dyslipidemia Model on Sprague Dawley Rats Fatchiyah Fatchiyah; Eko Suyanto; Rista Nikmatu Rohmah; Lidwina Faraline Triprisila; Hazna Noor Meidinna; Dewi Ratih Tirto Sari; Iva Himmatul Aliyah
Biotropika: Journal of Tropical Biology Vol 9, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2021.009.02.08

Abstract

The aim of this study was to make animal dyslipidemia models in Sprague Dawley strains induced by a high fat goat diet formula as hypercholesterol feed for two months. The experimental animal used in this study was 30 male rats (Rattus norvegicus strain Sprague Dawley) with an age of 2-3 months with an average body weight of 150g. Animal models are divided into two groups consisting of a control group without additional diet and dyslipidemia group given food consumption goat hypercholesterolemia with high-fat diet formula orally every day for two months. Physiological characteristics of dyslipidemia SD rats had higher body weight, increased food consumption and fecal weight, and decreased water intake and urine volume than the control group. Total cholesterol, triglyceride, and LDL-cholesterol levels increased, while HDL-cholesterol levels did not change in the dyslipidemia rats group compared to the control group. The conclusion of this study indicated that the hypercholesterol diet formula with a high composition of goat fat was successfully induced the SD rats to become dyslipidemia model rat with specific hypercholerol characteristics.
Predominant Bacterial Diversity in Rheumatoid Arthritis Rat After Treated with Caprine CSN1S2 Protein Eko Suyanto; Fatchiyah Fatchiyah
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.01

Abstract

Introduction: Rheumatoid arthritis (RA) is an autoimmune and systemic inflammatory disease influenced by microbial abnormalities (dysbiosis) in the intestinal that affect changes in metabolism and immune system disorders. This study aimed to investigate the predominant intestinal microbiota in complete Freund’s Adjuvant-induced rheumatoid arthritis rats after treated with caprine milk CSN1S2 protein through fecal analysis based on PCR-DGGE and to construct the phylogenetic tree of bacteria as the evolutionary relationship. Method: The experimental animals were divided into 6 groups with 2 types of rat model, namely control rat (C group, CM group, and CY group) and RA rat (RA group, RAM group, and RAY group). Predominant cultivable microbiota was obtained by direct culture and analyzed using PCR-DGGE with some specific primers. The DNA sequences were analyzed and aligned using bioinformatics software to construct the phylogenetic tree. Results: We found that Lactobacillus group significantly increased in the control rat model and the predominant intestinal bacteria in RA rats were Enterococcus group (Enterococcus faecium and Enterococcus faecalis). Conclusions: The caprine milk CSN1S2 protein influences the composition of microbiota in RA rats with the emergence of predominant bacteria that are considered species of the Bacillus group, closely related to Bacillus coagulans, which can be promoted the growth of B. coagulans to suppress pathogenic bacteria in the development of RA disease.
Pemberdayaan Kelompok Ibu PKK Desa dalam Penyuluhan Menu Nutrisi Sehat Berbasis Produk Lokal di Desa Karangwidoro, Kecamatan Dau, Kabupaten Malang Fatchiyah Fatchiyah; Eko Suyanto; Ernanin Dyah Wijayanti; Yeni Avidhatul Husnah
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 2, No 2 (2022)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2022.002.02.70

Abstract

Berdasarkan hasil penelitian riset kesehatan dasar, konsumsi pangan masyarakat Indonesia saat ini sebagian besar belum sesuai nutrisi sehat dan seimbang khususnya konsumsi sayuran dan buah-buahan. Hal ini menimbulkan kurangnya implementasi konsumsi pangan sehat masyarakat desa. Penyuluhan dan edukasi menu nutrisi sehat berbasis produk lokal menjadi penting dilakukan untuk mendukung Gerakan Masyarakat Hidup Sehat (GERMAS) serta pemanfaatan pangan lokal yang bernutrisi sehat. Salah satu desa dengan potensi sumber daya lokal yang melimpah sebagai produk lokal pangan nutrisi sehat adalah Desa Karangwidoro, Kecamatan Dau, Kabupaten Malang. Kegiatan penyuluhan ini merupakan upaya pemenuhan program pengabdian kepada masyarakat DPP/SPP Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya Tahun 2022 untuk memberikan edukasi bagi masyarakat khususnya ibu-ibu PKK sebagai penggerak pertama dalam keluarga. Metode pelaksanaan dilakukan dengan 5 tahap yaitu kerangka pemecahan masalah; diskusi; sosialisasi penyuluhan; khalayak sasaran yakni perangkat desa, ketua pokja sandang dan kesehatan, ibu-ibu PKK; serta pelaksanaan kegiatan. Bentuk penyuluhan berupa edukasi pangan sehat, praktik menu sehat dan pengolahannya, serta pemaparan materi. Kegiatan penyuluhan dan edukasi menu sehat berjalan sukses dengan ibu-ibu kader PKK desa yang sangat antusias. Hasil kegiatan pre-test menunjukkan nilai 65–70 sebanyak 33%. Hasil post-test terdapat peningkatan menjadi 91–100 sebanyak 45%. Pendampingan dan pemantauan dilakukan oleh kader PKK kesehatan dan tim pengabdi di masyarakat secara door to door untuk mendukung keberhasilan program.
Differential Intestinal Microbiota Composition Inhibits the Lactobacillus Growth in Rheumatoid Arthritis Patients in Malang, Indonesia. Mufidah Mufidah; Eko Suyanto; Viranda Sutanti; Hazna Noor Meidinna; Fatchiyah Fatchiyah
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.02

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease that can cause progressive damage to the joints of patients. The number of patients is expected to increase, along with the exact cause of this disease remains unknown. However, there are several risk factors associated with RA, including dysbiosis. The purpose of this study was to characterize the composition of intestinal microbiota in the RA and control groups through fecal analysis and reveal the association of microbiota composition with RA disease in Indonesia, especially Malang. Fecal samples were obtained from RA patients and controls. Fecal analysis was carried out through several stages, namely the calculation of total bacterial colonies, isolation and characterization of anaerobic bacteria, calculation of the Simpson diversity index, and DNA isolation. Analysis of bacterial composition profiles in fecal was carried out using 6 specific primer sets through PCR analysis. The results of the 16S rRNA PCR analysis showed different microbiota compositions between RA patients and controls. The number of Enterococcus bacterial group was lower in the control patients than the RA group, whereas the Lactobacillus bacteria decreased in RA patients. In addition, our study found that the existence of bacterial isolate 11 changed the composition of microbiota in RA patients, and the DNA band only appeared in Universal primers. The diversity of bacterial species can provide symbiotic and pathogenetic effects in RA patients.Keywords: Dysbiosis, intestinal microbiota, PCR, rheumatoid arthritis.
Pendampingan Penguatan Kompetensi Guru dan Siswa SMA Melalui Pengembangan Inovasi Sains dan Kompetisi Olimpiade Biologi di Kabupaten Sampang, Madura Eko Suyanto; Anna Safitri; Nia Kurnianingsih; Fatchiyah Fatchiyah
Engagement: Jurnal Pengabdian Kepada Masyarakat Vol 4 No 2 (2020): November 2020
Publisher : Asosiasi Dosen Pengembang Masyarajat (ADPEMAS) Forum Komunikasi Dosen Peneliti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/engagement.v4i2.288

Abstract

Teachers and students need more knowledge to improve their competence. The educational quality can be improved by varying learning methods and supported by competition. This community service activity aims to strengthen the competence of teachers and students through learning innovation in the Biology subject and to provide learning competition of Biology Olympiad through simulation and training. The community-based research (CBR) method was performed by workshop and training involving 60 participants. As many as 100% of the participants attended the activities. The results indicated that the participants acquired new knowledge and realized their competence skills. Learning innovation and competition were able to improve their knowledge and competence. The participants are eager to apply the methods learned at school. These new competence and skill are expected to improve the quality of education
Co-Authors Abdurahman Abdurrahman Adhya Dava Aligarh Yahya Afis Pratama, Afis Agus Suyatna Andra, Doni Ayuningtias, Meta Dwi Chandra Ertikanto Destryati, Annisya Dewi Ratih Tirto Sari ENDANG SUTARININGSIH SOETARTO Ernanin Dyah Wijayanti Ernanin Dyah Wijayanti Ezra, Achmad Fahrurrozi Fahrurrozi fatchiyah . Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatma Yona, Hafidza Gusmawati, Niken F. Hasan, Muhammad Fikri Hazna Noor Meidinna Hazna Noor Meidinna Hervin Maulina, Hervin Hose, Victor Alvianoes Guterez Husnah, Yeni Avidhatul I Dewa Putu Nyeneng I Wayan Distrik Iva Himmatul Aliyah Karuniasari, Nadaa Khairunnisa Hidaya, Amira Khasana, Intan Lidwina Faraline Triprisila Miranti Nurindah Sari, Miranti Nurindah Mufidah Mufidah Muhammad Nur Cahyanto Murwantoko . Muwaffiq Faza, Ahmad Neni Hasnunidah Novi Kurnianingsih Novinta Nurulsari Nwineh, Legborsi Pratama, Ardo Cahya Putri, Indah Ardita Rahmadini, Agnia Fadillah Rista Nikmatu Rohmah Safira, Safira Safitri, Anna Sari, Witri Puspita Setiawati, Santy Shafala Safa, Muhammad SHANTI RATNAKOMALA Talitha Pangestu, Twistka Turhadi Turhadi Undang Rosidin Viranda Sutanti Viyanti Viyanti Wijaya, Karina Yasir Saifur Rahman Yeni Avidhatul Husnah