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STUDI IN VITRO SENYAWA BIOAKTIF EKSTRAK DAN FRAKSI DAUN SIRIH MERAH (Piper crocatum) SEBAGAI INHIBITOR α-GLUKOSIDASE Mustika Weni; Mega Safithri
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 8, No 1 (2022): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Daun sirih merah (Piper crocatum) dimanfaatkan oleh masyarakat sebagai tanaman obat. Penelitian ini bertujuan menentukan efektivitas aktivitas ekstrak etanol, fraksi etil asetat, fraksi n-heksan dan fraksi air daun sirih merah (Piper crocatum) dalam menghambat enzim alfa glukosidase dan menentukan tipe penghambatannya terhadap enzim alfa glukosidase dengan metode Dixon. Ekstraksi dilakukan dengan menggunakan pelarut etanol 70%. Ekstrak etanol kemudian di fraksinasi dengan menggunakan 3 pelarut dengan tingkat kepolaran yang berbeda, yaitu air, etil asetat dan n-heksan. Penentuan aktivitas penghambatan enzim alfa glukosidase dengan metode Dixon. Hasil penelitian menunjukkan fraksi etil asetat daun sirih merah memiliki aktivitas penghambatan paling baik dibandingkan dengan ekstrak etanol 70%, fraksi n-heksan dan fraksi air. Nilai IC50 yang diperoleh fraksi etil asetat daun sirih merah sebesar 743.80 μg/mL, mekanisme penghambatan yang diperoleh adalah penghambatan kompetitif dengan nilai Ki sebesar 95 μM. Kata Kunci: Aktivitas penghambatan enzim α-glukosidase, Dixon, Piper crocatum Piper crocatum is used by the community as a medicinal plant. This study aimed to determine the effectiveness of the activity of ethanol extract, ethyl acetate fraction, n-hexane fraction and water fraction of red betel leaf (Piper crocatum) in inhibiting the alpha glucosidase enzyme and determine the type of inhibition of the alpha glucosidase enzyme using the Dixon method. Extraction was carried out using 70% ethanol as a solvent. The ethanol extract was then fractionated using 3 solvents with different polarity levels, namely water, ethyl acetate and n-hexane. Determination of the inhibitory activity of the alpha glucosidase enzyme by the Dixon method. The results showed that the ethyl acetate fraction of red betel leaf had the best inhibitory activity compared to 70% ethanol extract, n-hexane fraction and water fraction. The IC50 value obtained by the ethyl acetate fraction of red betel leaf was 743.80 g/mL, the inhibitory mechanism obtained was competitive inhibition with a Ki value of 95 M. Keywords: Inhibitory activity of -glucosidase enzymes, Dixon, Piper crocatum

Antibacterial Activity Test of Ethanol Extract from Jackfruit Leaves (Artocarpus heterophyllus) on Escherichia coli Bacterial Growth Sri Marfuati*; Mustika Weni
Riwayat: Educational Journal of History and Humanities Vol 6, No 3 (2023): Social, Political, and Economic History
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jr.v6i3.33578

Infectious diseases continues to grow in the health sector due to the colonization of infectious organisms and is the main cause of death worldwide. Infection can be caused by bacteria, fungi, viruses and parasites. One example of bacteria that can cause infection is Escherichia coli. Jackfruit (Artocarpus heterophyllus) have been used by communities for to treat or handle various diseases. This study aims to determine the antibacterial activity from ethanol Jackfruit Leaves against the growth inhibition of the bacterium Escherichia coli using three concentrations of 12,5%, 25% and 50%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the well diffusion method. This results of this study indicate that ethanol extracts of Jackfruit leaves are considered as antibacterial and have antibacterial strength against Escherichia coli at concentrations of 50% include strong groups to inhibit Escherichia coli, while at a concentration of 12,5% and 25% include medium groups to inhibit Escherichia coli.

Antibacterial Activity Test of Ethanol Extract from Jackfruit Leaves (Artocarpus heterophyllus) on Escherichia coli Bacterial Growth Sri Marfuati*; Mustika Weni
Riwayat: Educational Journal of History and Humanities Vol 6, No 3 (2023): Social, Political, and Economic History
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jr.v6i3.33578

Infectious diseases continues to grow in the health sector due to the colonization of infectious organisms and is the main cause of death worldwide. Infection can be caused by bacteria, fungi, viruses and parasites. One example of bacteria that can cause infection is Escherichia coli. Jackfruit (Artocarpus heterophyllus) have been used by communities for to treat or handle various diseases. This study aims to determine the antibacterial activity from ethanol Jackfruit Leaves against the growth inhibition of the bacterium Escherichia coli using three concentrations of 12,5%, 25% and 50%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the well diffusion method. This results of this study indicate that ethanol extracts of Jackfruit leaves are considered as antibacterial and have antibacterial strength against Escherichia coli at concentrations of 50% include strong groups to inhibit Escherichia coli, while at a concentration of 12,5% and 25% include medium groups to inhibit Escherichia coli.

Antioxidant Activity and Tyrosinase Inhibitor of Red Betel Leaf Extract Mustika Weni; Mega Safithri
Indonesian Journal of Applied Research (IJAR) Vol. 4 No. 3 (2023): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v4i3.374

Red betel leaf (Piper Crocatum) contains flavonoids, tannins, steroids, and alkaloids, known to act as antioxidants. This study aimed to determine the antioxidant activity of red betel leaf extract. The solvents used for the extraction process are ethanol and n-hexane. The thiobarbituric acid (TBA) method was used to determine antioxidant activity. The results showed that the extract of 200 ppm inhibited fatty acid oxidation by 52.13%. No significant difference (α = 0.05) inhibition of unsaturated fatty acids oxidation between the sample and α-tocopherol at 200 ppm. The ethanol extract of red betel can inhibit the tyrosinase enzyme higher than the n-hexane extract of red betel, as seen from the IC50 value of the ethanol extract of red betel of 1655 ppm, while the IC50 value of n-hexane of red betel is 3090.56 ppm.

ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT OF KERSEN LEAF (Muntingia calabura L.) TOWARDS Escherichia coli BACTERIA Sri Marfuati; Mustika Weni; Emilia Putri Agustin
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.953

Escherichia coli is a bacteria that causes infection. In Indonesia, it has been reported that Escherichia coli is resistant to several types of antibiotics; therefore, other alternatives are needed to inhibit the growth of this Escherichia coli. One alternative method is to use medicinal plants, such as cherry leaves, which contain flavonoids, saponins, and tannins as antibacterials. Aim to determine the antibacterial activity of an ethanol extract of cherry leaves (Muntingia calabura L.) against Escherichia coli bacteria. The research used a post-test-only control group design. This study contained 7 groups: 1 positive control group, 1 negative control group, and 5 treatment groups. The treatment group consisted of an ethanol extract of cherry leaves with concentrations of 1%, 2%, 4%, 8%, and 10%. The control group was a positive control (K(+) with co-trimoxazole and a negative control (K(-) namely dimethyl sulfoxide (DMSO) at 10%. The data used was One-way ANOVA. The results of this study were the average zone of inhibition. The largest is at a concentration of 10%, namely 6 mm, while the smallest average inhibition zone is at a concentration of 4%, namely 4.37 mm. Concentrations of 2% and 1% do not indicate the presence of an inhibition zone. From these results it is concluded that there are significant differences between The concentration of cherry leaf ethanol extract against Escherichia coli bacteria and the minimum inhibitory level of cherry leaf ethanol extract against Escherichia coli bacteria is at a concentration of 4% with an average inhibition zone of 4.37mm.

Antimicrobial Activity of Ethyl Acetate Fraction of Kersen Leaves Against Staphylococcus aureus and Escherichia coli Weni, Mustika; Marfuati, Sri
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.531

The underutilized leaves of the kersen plant contain secondary metabolite compounds that have antibacterial potential. The aim of this research was to test the antibacterial activity of the ethyl acetate fraction of kersen leaf against Escherichia coli and Staphylococcus aureus and determine the best concentration. Kersen leaf extraction method using the maceration method using ethanol solvent, fractionation method using ethyl acetate solvent and testing the antibacterial activity of kersen leaf against Staphylococcus aureus and Escherichia coli using the well diffusion method. The normality test used was the Shapiro-Wilk test and used the One-way Anova parametric statistical test. The results of the phytochemical screening of kersen leaf contain compounds phenols, saponins, tannins, alkaloids and triterpenoids. Concentration of 20% of the ethyl acetate fraction of kersen leaf can inhibit Escherichia coli by 8,817 mm in the medium category and Staphylococcus aureus by 7,987 mm in the medium category. The inhibitory power of the ethyl acetate fraction is higher in inhibiting Escherichia coli than Staphylococcus aureus.

Dampak Pelatihan Kesehatan Gigi dan Mulut Pada Siswa Sekolah Dasar Dalam Meningkatkan Status Kebersihan Mulut Siswa Weni, Mustika; Mauladani , Muhammad Firqin; Wahidan, Fikri; Salsabila, Wanda Ayu; Yuniarni, Ervina
JPM: Jurnal Pengabdian Masyarakat Vol. 5 No. 4 (2025): April 2025
Publisher : Forum Kerjasama Pendidikan Tinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jpm.v5i4.2350

Dental caries is one of the most common oral health problems in society. One of the factors suspected to contribute to the occurrence of dental caries is the habit of brushing teeth. This program aims to increase students' knowledge about the importance of dental and oral care and train them in correct tooth brushing techniques. This activity began with counseling on dental health problems that children often face, such as cavities due to bad habits and high sugar consumption. Furthermore, students were given practical training on how to brush their teeth effectively. After the counseling, data collection was continued with a questionnaire to determine children's tooth brushing habits and the incidence of tooth decay. The results of the training showed that students were very enthusiastic and were able to understand and practice the correct tooth brushing technique. The results of the study: the habit of brushing teeth of elementary school children in the category of irregular brushing of teeth was 15 students (34.1%) of respondents from 44 respondents who were sampled. And the occurrence of tooth decay in children was 28 students (63.6%) of 44 respondents who were sampled. The results of the analysis of the relationship between tooth brushing habits and the occurrence of tooth caries. The analysis using the Chi-square test showed a P-Value = 0.361, indicating no significant relationship between tooth-brushing habits and the occurrence of dental caries. This suggests that other factors, such as dietary patterns, particularly frequent consumption of sugary foods like chocolate or candy, may have a greater influence on dental caries occurrence. Therefore, it is necessary to educate students about the importance of maintaining oral hygiene and reducing sugary food consumption to prevent dental caries.

Dampak Optimalisasi Kesehatan Remaja dengan Edukasi Bijak Dalam Penggunaan Obat Kepada Siswa SMP Marfuati, Sri; Weni, Mustika; Brajawikalpa, Rama Samara; Emallia Fitriani
JPM: Jurnal Pengabdian Masyarakat Vol. 6 No. 1 (2025): July 2025
Publisher : Forum Kerjasama Pendidikan Tinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jpm.v6i1.2444

Self-medication is increasingly common among the general public, including adolescents. However, a lack of proper understanding regarding drug use, storage, and disposal can lead to medication errors that pose serious health risks. This community service activity aimed to improve the knowledge of students at SMP regarding the proper and responsible use of medications through an educational session titled “Using Medicines Wisely.” The activity was conducted on May 24, 2025, by lecturers from the Faculty of Medicine, Universitas Swadaya Gunung Jati (UGJ), using a face-to-face method involving a pre-test, educational material delivery, interactive discussion, and a post-test. Pre-test results showed that only 22% of participants had good knowledge, which increased to 68% after the educational session with the meaning of value. These findings indicate that the activity effectively enhanced students’ understanding of responsible self-medication practices, particularly in recognizing drug types, usage instructions, proper storage, and safe disposal methods. This program is expected to foster wise medication habits from an early age and contribute to the development of a health-conscious society.

INDONESIA KARAKTERISTIK KLINIS INFEKSI PSEUDOMONAS AERUGINOSA DI RUMAH SAKIT GUNUNG JATI Rachman, Faqih Oktavian; Erwin I, Mohamad; Weni, Mustika
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 2 (2025): AGUSTUS 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i2.44116

Angka kejadian infeksi di Indonesia nosokomial mencapai 15,74%, jauh lebih tinggi dibandingkan negara-negara maju yang berkisar antara 4,8–15,5%. Tingkat mortalitas akibat infeksi Pseudomonas aeruginosa dilaporkan sebesar 15,1%. Penelitian ini bertujuan untuk mengetahui karakteristik klinis pasien dengan infeksi Pseudomonas aeruginosa. Jenis penelitian ini adalah observasional deskriptif dengan teknik total sampling, sehingga diperoleh sebanyak 153 sampel. Dari 153 pasien yang terinfeksi Pseudomonas aeruginosa, mayoritas berjenis kelamin laki-laki (56,9%) dengan kelompok usia balita (0–5 tahun) sebagai kelompok usia terbanyak (34,0%). Tindakan medis yang paling sering dilakukan adalah operasi pada saluran cerna dan abdomen (4,6%). Penggunaan alat invasif, terutama infus intravena, ditemukan pada 136 pasien (35,7%). Diagnosis klinis primer yang paling sering ditemukan adalah gagal napas (17,0%), sedangkan diagnosis sekunder terbanyak adalah bronkopneumonia (6,5%). Sebagian besar pasien (89,7%) tidak memiliki penyakit penyerta. Angka kematian akibat infeksi Pseudomonas aeruginosa dalam penelitian ini mencapai 58,2%, dengan rerata lama perawatan selama 5 hari. Di RSUD Gunung Jati, dari 153 kasus infeksi yang tercatat, kelompok usia balita dan lansia merupakan kelompok paling rentan, dengan dominasi pasien laki-laki (56,9%). Infeksi ini berkaitan erat dengan penyakit infeksi saluran pernapasan akut (ISPA), infeksi saluran kemih (ISK), infeksi luka, serta penggunaan alat medis invasif seperti infus intravena dan ventilator. Sebagian besar pasien juga menjalani tindakan pembedahan, dan beberapa di antaranya memiliki komorbiditas seperti diabetes mellitus, penyakit jantung, dan penyakit paru kronik.

Overview of COVID-19 Test Results (2021–2022) at the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia Febrianti, Fitri Ayu; Khasanah, Uswatun; Weni, Mustika
GHMJ (Global Health Management Journal) Vol. 7 No. 3s (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-741097

Background: COVID-19 (Coronavirus Disease-19) is a respiratory infection caused by the SARS-CoV-2 virus. In 2020, the World Health Organization (WHO) declared COVID-19 a global pandemic due to the rapid rise in cases and mortality rates worldwide. The pandemic has impacted millions of people and continues to pose a significant global public health threat. Accurate diagnosis of the disease depends on laboratory testing, with RT-PCR widely recognized as the gold standard for confirming COVID-19. Aims: To describe an overview of COVID-19 test results at Laboratory Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia, from 2021-2022. Methods: This study used a descriptive observational method. The sample was collected in July 2024 using a total sampling technique. The sample includes all 135,713 patients who had COVID-19 tests at Laboratory Faculty of Medicine Swadaya Gunung Jati Univesity between 2021 and 2022. Data were collected from medical records of COVID-19 test. The variables of this study include gender, age, and COVID-19 test results. A statistical analysis was used to determine frequency distribution. Results: The results of the study showed that among the sample who tested for COVID-19, the majority are male (50.9%) and early adolescent age group (23.9%). RT-PCR test findings were positive in 16.3% of patients and negative in 83.7% of patients. Among PCR positive cases, the majority are female (17.4%), with seniors age groups >65 years accounting for 39.0%. Conclusion: Most of the tested samples are males and early adolescent age groups, with more negative PCR results than positive ones. Among positive cases, the highest number was found in females and seniors age group. It is recommended that females and seniors age groups keep protected during COVID-19 outbreaks. Received: 25 September 2024 | Reviewed: 22 October 2024 | Revised: 30 November 2024 | Accepted: 11 December 2024.

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