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All Journal Bioscientist : Jurnal Ilmiah Biologi JUSTER: Jurnal Sains dan Terapan
Arti Wahyu Utami
Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Energy Immunology & microbiology Physics Social Sciences

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Estimasi Populasi Bakteri INA (Ice Nucleation Active) Pada Tumbuhan Berdaun Jarum Di Cemoro Sewu Gunung Lawu Arti Wahyu Utami
JUSTER : Jurnal Sains dan Terapan Vol. 1 No. 3 (2022): JUSTER: Jurnal Sains dan Terapan
Publisher : Jompa Research and Development

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Abstract

Bakteri Ice Nucleation Active (INA) merupakan penyebab frost injury atau luka beku pada tanaman. Habitat bakteri INA berada pada permukaan daun atau philosper. Bakteri INA berperan dalam proses biopresipitasi yaitu menginisiasi pembentukan kristal es pada awan di troposfer.. penelitian bakteri INA untuk saat ini masih banyak dilakukan di negara subtropis. Oleh karena itu, penelitian tentang bakteri INA dari daerah pegunungan tropis khususnya di Indonesia perlu dilakukan untuk mengetahui keberadaan bakteri INA di negara tropis khususnya Indonesia. Penelitian ini bertujuan untuk mengestimasi populasi bakteri INA pada tumbuhan berdaun jarum  yaitu Pinus merkussi, Cupresus sp dan Casuarina sp di jalur pendakian Cemoro Sewu Gunung Lawu. Sampel tumbuhan diambil dari jalur pendakian Cemoro Sewu dengan masing-masing ketinggian: 2.300; 2.500; dan 2.700 m.dpl. Metode yang digunakan untuk mengetahui populasi bakteri INA adalah metode multiple nucleation tube dan menggunakan alat  circulating alcohol bath. Jumlah sel populasi bakteri INA/g berat segar daun tumbuhan berdaun jarum diperkirakan berdasarkan tabel Most Probable Number (MPN). Hasil penelitian menunjukkan bahwa bakteri INA ditemukan pada tumbuhan berdaun jarum di jalur pendakian Cemoro Sewu Gunung Lawu . Populasi bakteri INA pada Pinus merkussi <3,0x103 sel/g, Cupressus sp 3,6 x103 sel/g, dan Casuarina sp <3,0x103 sel/g. Hal ini menunjukkan bahwa populasi bakteri INA yang terdapat pada tumbuhan berdaun jarum di Gunung Lawu relatif rendah.
Isolation and Characterization of Endophytic Bacteria from Curcuma Aeruginosa Roxb As Antibacterial Candidates Againts Pneumonia Arti Wahyu Utami; Firman Rezaldi; Rifkarosita Putri Ginaris; Jihan Nabilla; Bramantyo; Puput Setiawati; Zulfania Shandra Fatikha; Nanik Pujiastuti; Dwi Wahyuni
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18148

Abstract

This study aimed to isolate and characterize endophytic bacteria from black turmeric rhizomes (Curcuma aeruginosa Roxb) and test their potential as antibacterial agents against Streptococcus pneumoniae. This experimental research began with the isolation of endophytic bacteria from surface-sterilized black turmeric rhizomes. The obtained pure isolates were characterized macroscopically (colony shape, margin, elevation, pigment) and microscopically (cell shape, Gram staining). The growth curve of potential isolates was determined, and antibacterial activity was tested using the disc diffusion method against S. pneumoniae, with ciprofloxacin as a positive control. Six endophytic bacterial isolates (PS1, PS2, PS3, PS4, PS5, PS6) were successfully isolated, showing variations in colony morphology, and all were classified as Gram-negative bacilli. All isolates exhibited strong antibacterial activity against Streptococus pneumoniae, with inhibition zone diameters ranging from 15.3 mm to 20.6 mm. Black turmeric rhizomes (Curcuma aeruginosa Roxb) harbor diverse endophytic bacteria with significant potential as antibacterial agents against the pneumonia pathogen, Streptococcus pneumoniae.
Co-Authors Bramantyo DWI WAHYUNI Jihan Nabilla Nanik Pujiastuti Puput Setiawati Rifkarosita Putri Ginaris Zulfania Shandra Fatikha