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All Journal Indonesian Journal of Chemical Science Indonesian Journal of Chemical Science
Mohammad Alauhdin
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Chemical Engineering, Chemistry & Bioengineering Chemistry Physics

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Calibration of Microplate Uv-Vis Spectrophotometer for Quality Assurance Testing of Vitamin C using Calibration Curve Method Martin Sulistyani; Nuril Huda; Ridho Prasetyo; Mohammad Alauhdin
Indonesian Journal of Chemical Science Vol 12 No 2 (2023)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v12i2.72451

Abstract

Spektrofotometer UV-Vis Fluostar Omega Tipe Mikroplate di laboratorium Kimia FMIPA UNNES telah dipakai untuk praktikum, penelitian dan layanan pengujian sampel. Untuk menjamin hasil pengukuran yang valid, kalibrasi spektrofotometer tersebut perlu dilakukan secara berkala. Pada penelitian ini, kalibrasi dan verifikasi kinerja instrumen spektrofotometer UV- Vis Fluostar Omega Tipe Mikroplate dilakukan untuk penjaminan mutu pengujian kadar sampel. Sampel yang dianalisis adalah vitamin C dengan metode kurva kalibrasi. Kalibrasi yang dilakukan meliputi kalibrasi fotometri, dan kalibrasi panjang gelombang. Selanjutnya, validasi metode dan pengujian kadar vitamin C dalam sampel juga dilakukan. Akurasi fotometri pada daerah ultraviolet dan visibel serta akurasi panjang gelombang menunjukkan Persentase RSD < 2%. Nilai ini membuktikan bahwa pembacaan absorbansi dan panjang gelombang oleh alat sangat teliti. Uji lineritas standar vitamin C didapatkan persamaan linear y = 0,0164x + 0,1128 dengan nilai koefesien korelasi(R2) sebesar 0.9982. Validasi metode pengujian vitamin C didapatkan rata-rata persen galat, persen recovery, dan persen akurasi masing-masing sebesar 5,6784%, 94,3216%, dan 94,3216%, dengan kategori sangat teliti. Pengujian kadar sampel tablet vitamin C 500 mg didapatkan hasil yang sangat teliti dengan persen galat, persen recovery, dan persen akurasi masing-masing sebesar 8,2565%, 91,7435%, dan 91,7435%.
Biopolymers of Chitosan-Anthocyanin From Purple Sweet Potatoes (Ipomoea Batatas L.) Extract and Their Interaction with Formalin Riska Putri Mawarni; F. Widhi Mahatmanti; Harjono; Mohammad Alauhdin
Indonesian Journal of Chemical Science Vol. 13 No. 1 (2024)
Publisher : Prodi Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v13i1.3865

Abstract

Purple sweet potato is one of the potential sources of anthocyanins. Anthocyanins can be utilized in various fields, such as sensors. Chitosan biopolymer allows formulation with anthocyanins because it possesses biodegradable and antimicrobial properties, and it does not inhibit color changes from anthocyanins. This study aims to prepare anthocyanins from purple sweet potatoes, formulate chitosan-anthocyanin biopolymer (CAB), and test the effect of time and formalin concentration on color changes in CAB. The research results show: 1) anthocyanin extract (TAC) from purple sweet potatoes is 852 mg/L with anthocyanin monomer (cyanidin) of 443.03 mg/500 g or 88.61 mg/100g. Anthocyanin extract at pH 1-12 has colors ranging from red to brownish-yellow; 2) CAB was successfully formulated with anthocyanin extract from purple sweet potatoes, chitosan, and glycerin. BKA-A was obtained with a content of 10 mL anthocyanin extract and CAB-B with 15 mL anthocyanin extract; 3) the produced biopolymers have average swelling values of 444% (CAB-A) and 339.5% (CAB-B). The storage time of CAB-B is longer than CAB-A. FTIR analysis showed a broad peak at ~3336 cm-1 indicating hydroxyl groups from anthocyanin extract; and 4) ANOVA test results showed that interaction time and formalin concentration affect color changes in CAB-A partially, while color changes in CAB-B are only influenced by interaction time with formalin.
Co-Authors F. Widhi Mahatmanti Harjono Martin Sulistyani Nuril Huda Ridho Prasetyo Riska Putri Mawarni