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All Journal Animal Production : Indonesian Journal of Animal Production Jurnal Kedokteran Hewan
Soeparna Soeparna, Soeparna
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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The Effect of Caffeine Supplementation in Egg-Yolk Tris Extender on Motility and Velocity of Garut Sheep Sperm Separation Rasad, SD; Soeparna, Soeparna; Nurfaidah, L
ANIMAL PRODUCTION Vol 12, No 3 (2010): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.657 KB)

Abstract

The aims of this research were to know the effect of caffeine in Tris egg yolk extender on motility and velocity of Garut Ram sperm after separation and to find out the optimum dosage of caffeine for both parameters. This research used experimental design with Completely Randomized Design (CRD) with four treatments on different dosage  (0 mM, 2 mM, 4 mM and 6 mM), each treatment is repeated five times. The result showed that the supplemtation of caffeine significantly influence sperm motility  of Garut Ram sperm after separation. The highest sperm motility was on dosage 4 mM that was 63.57%  on top fraction and 65.90% on below fraction. The highest sperm velocity was on dosage 4 mM that was 62.37 µm/second on top fraction and 64.24 µm/second on below fraction and significantly influence sperm velocity. The conclusion, 4 mM dosage of caffeine  was the optimum dosage to reach the highest motility and velocity of Garut Ram sperm both on top  and below fractions. (Animal Production 12(3): 139-143 (2010)Key Words: spermatozoa, Garut ram, sperm separation, caffeine
Effect of PMSG administration in combination with vaginal sponge on estrous occurrence and litter size of Javanese sheep Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100% of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively. Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep
Co-Authors L Nurfaidah, L Nurcholidah Solihati Rangga Setiawan Rini Widyastuti SD Rasad, SD Siti Darodjah Rasad