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All Journal Buletin Peternakan BIOTROPIA - The Southeast Asian Journal of Tropical Biology agriTECH Biosaintifika: Journal of Biology & Biology Education

Ria Millati

Departemen Teknologi Pangan Dan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281

Author-ID : 654705

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary Other

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Pengaruh Ukuran Potongan terhadap Pertumbuhan Jamur Pleurotus floridanus LIPIMC 996 dan Hasil Delignifikasi Selama Perlakuan Pendahuluan Tandan Kosong Kelapa Sawit Lukitawesa Lukitawesa; Ria Millati; Muhammad Nur Cahyanto
agriTECH Vol 32, No 4 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The aim of this research was to determine the effects of particle size on the growth of Pleurotus floridanus LIPIMC 996 and the delignification of oil palm empty fruit bunch (OPEFB) during pretreatment. The OPEFB was reduced its size to 0.5, 1, 2, 4, and 8 cm, sterilized at 121oC for 15 minutes, inoculated with P. floridanus LIPIMC 996 mycelia, and incubated in room temperature for 35 days. The lignin, cellulose, hemicellulose, and glucosamine content of OPEFB were analyzed every 7 days. Size reduction of OPEFB from 8 cm to 2 cm reduced the growth rate of P. floridanus LIPIMC 996 and degradation rate of lignin and cellulose, but reduction from 2 cm to 1 cm increased the fungal growth rate. The lignin and cellulose degradation rate from the various different fiber sizes showed the same trend. The elimination of lignin reached maximum 79.7% on day-35 for 8 cm-long OPEFB. The maximum of cellulose and hemicelluloses degradation were 78.9% and 80.6%, respectively.ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh ukuran potongan terhadap pertumbuhan jamur Pleurotus floridanus LIPIMC 996 dan hasil delignifikasi selama perlakuan pendahuluan tandan kosong kelapa sawit (TKKS). TKKS dikecilkan ukurannya menjadi 0,5; 1; 2; 4; dan 8 cm, disterilisasi pada suhu 121oC selama 15 menit, diinokulasi dengan miselia P. floridanus LIPIMC 996 kemudian diinkubasi pada suhu ruang selama 35 hari. Setiap 7 hari, dilakukan pengambilan sampel untuk analisis lignin, selulosa, hemiselulosa, dan glukosamin. Hasil penelitian menunjukkan bahwa pengecilan ukuran TKKS dari 8 cm hingga 2 cm memperlambat laju pertumbuhan jamur serta laju degradasi lignin dan selulosa sedangkan pada ukuran 1 cm dan 0,5 cm justru mempercepat. Pola laju degradasi lignin dan selulosa pada setiap ukuran potongan TKKS sama dengan pola laju pertumbuhan pada setiap ukuran potongan. Penurunan lignin terbesar terjadi pada TKKS dengan panjang 8 cm yaitu mencapai 79,7%. Degradasi selulosa mencapai 78,9% pada TKKS dengan panjang 8 cm. Selain itu, degradasi hemiselulosa mencapai 80,6% pada TKKS dengan ukuran 8 cm.

Potensi Lactobacillus plantarum yang Diisolasi dari Dadih dalam Meningkatkan Kadar Folat Susu Fermentasi Siti Nur Purwandhani; Tyas Utami; Ria Millati; Endang Sutriswati Rahayu
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Folate is a B vitamin that participates in one-carbon transfer reactions of metabolism process, particularly purine and pyrimidine biosynthesis (DNA and RNA). Some strains of lactic acid bacteria are known to produce folic acid through the conversion of guanosine tri phosphate and the presence of precursor p-amino benzoic acid and glutamic acid. In this study, skim milk was fermented using Lactobacillus plantarum Dad-13, G-3, and H-1 to increase the levels of folate. Fermentation was conducted at 37 °C for 18 hours. The aims of this study were to investigate the population of L. plantarum during fermentation period, the change in pH and the levels of folate in fermented milk. The results showed that during the fermentation period using L. plantarum G-3, H-1, and Dad-13 cell count increased from 107 to 108 for G-3 and H-1, while Dad-13 into 109. The pH value decreased and the levels of folic acid in early fermentation increased rapidly but after approaching the end of fermentation, it began to stagnate. The folate level of skim milk was 23.70 ± 3.25 µg/L. Increased levels of folate in fermented milk after 18 hours fermentation using L. plantarum Dad-13, G-3, and H-1 were 32.04 ± 1.85 µg/L (135.19%), 28.21 ± 0.28 µg/L (118.99%), and 25.13 ± 1.27 µg/L (106.03%), respectively.ABSTRAKFolat merupakan vitamin B yang berpartisipasi dalam reaksi transfer satu-karbon dalam proses metabolisme, terutama biosintesis purin dan pirimidin (DNA dan RNA). Beberapa strain bakteri asam laktat diketahui mampu memproduksi asam folat melalui konversi guanosin tri pospat dan dengan adanya prekursor p-amino benzoat serta asam glutamat. Pada penelitian ini, susu skim difermentasi menggunakan Lactobacillus plantarum Dad-13, G-3, dan H-1 untuk meningkatkan kadar asam folatnya. Fermentasi dilakukan pada 37 °C selama 18 jam. Tujuan penelitian ini adalah untuk mengetahui pola pertumbuhan sel, perubahan pH, dan kadar asam folat susu selama fermentasi. Hasil penelitian menunjukkan bahwa selama fermentasi menggunakan L. plantarum Dad-13, G-3, dan H-1 jumlah sel meningkat dari 107 menjadi 108 untuk G-3 dan H-1, sedangkan Dad-13 menjadi 109. Nilai pH mengalami penurunan dan kadar asam folat pada awal fermentasi meningkat dengan cepat tapi setelah mendekati akhir fermentasi mulai stagnan. Kadar asam folat susu skim adalah 23,70 ± 3,25 µg/L. Dibandingkan dengan kadar asam folat susu skim, peningkatan kadar folat susu fermentasi setelah 18 jam fermentasi dengan starter L. plantarum Dad-13, G-3, dan H-1 secara berturut-turut 32,04 ± 1,85 µg/L (135,19%), 28,21 ± 0,28 µg/L (118,99%), dan 25,13 ± 1,27 µg/L (106,03%).

Effect of Hydraulic Retention Time (HRT) and Organic Loading Rate (OLR) to the Nata de coco Anaerobic Treatment Eficiency and its Wastewater Characteristics Istna Nafi Azzahrani; Fanny Arivia Davanti; Ria Millati; Muhammad Nur Cahyanto
agriTECH Vol 38, No 2 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

In this study, experiments were conducted to investigate the effect of hydraulic retention time (HRT) and organic loading rate (OLR) on process stability of nata de coco wastewater anaerobic treatment using semi-continuous digester. The standard-rate anaerobic digester with working volume of 8.5 L was used to investigate the effect of three different hydraulic retention times (15, 20, and 25 days), and a standard-rate anaerobic digester with working volume of 9.1 L was operated at different organic loading rates of 0.5, 1, and 1.5 g/L/day. The findings revealed that minimum HRT for nata de coco wastewater anaerobic treatment using semi-continuous digester was achieved at HRT 20 days. Based on data from this study, the reduction of organic content in nata de coco wastewater increased when OLR increased until 1 g/L/day. But then those parameters value decreased when OLR being increased further to 1.5 g/L/day. It showed that at 1.5 g/L/day the amount of substrate fed to the system was exceeding the total degradation capacity of methanogenic microorganisms, hence the organic overload happened and decreased the efficiency of organic content reduction in anaerobic treatment of nata de coco wastewater.

Optimization of Crude Palm (Elaeis guineensis) Oil Bleaching using Zeolite-Fe by Response Surface Methodology Ulfah Anis; Ria Millati; Chusnul Hidayat
agriTECH Vol 42, No 1 (2022)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

A carcinogenic 3-monochloropropane 1, 2 diol ester (3-MCPD ester) can be formed during processing crude palm oil (CPO). Chlorine is one of the precursors for the formation of 3-MCPD esters. This study aimed to optimize the bleaching conditions using zeolite-Fe for reducing chlorine concentration by the Response Surface Methodology (RSM) and further evaluate the characteristics of the bleached CPO. Factors such as bleaching time, zeolite-Fe concentration, and bleaching temperature were evaluated and further optimized. The results showed that Fe in the modified zeolite-Fe increased about 71.89% compared to natural zeolite. Zeolite-Fe concentration, bleaching time, and bleaching temperature had a significant effect on chlorine adsorption. The optimum bleaching process was obtained at a zeolite-Fe concentration of 5% (w/w) and bleaching temperature of 80 °C for 30 minutes. Bleached CPO had a chlorine concentration of 25 ± 1 ppb, carotenoid of 467.70 ± 13.71 ppm, and a DOBI (Deterioration Of Bleachability Index) value of 2.17 ± 0.01 R.

Co-Authors Chusnul Hidayat Chusnul Hidayat Engkus Ainul Yakin Fanny Arivia Davanti Indrati, Retno Istna Nafi Azzahrani Lukitawesa Lukitawesa Muhammad Nur Cahyanto Pramesti Dewi Rahayu, Endang Sutriswati Ristianto Utomo Sardjono Sardjono Siti Nur Purwandhani Teguh Ariyanto Tyas Utami Ulfah Anis Umiyati, Rini Zaenal Bachruddin

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