Tjahjadi Purwoko
Program Studi Biologi - Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sebelas Maret Surakarta

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KUALITAS FISIKA, KIMIA, DAN MIKROBIOLOGI AIR TAP UNIVERSITAS SEBELAS MARET SURAKARTA Yovanisa Meirawati; Tjahjadi Purwoko; Ratna Setyaningsih
Biospektrum Jurnal Biologi Vol 1, No 2 (2023): Biospektrum Jurnal Biologi
Publisher : Program Studi Biologi - Universitas Katolik Widya Mandala Surabaya (kampus Kota Madiun)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/bios.v1i2.1249

Abstract

Universias Sebelas Maret (UNS) Surakarta has a piped drinking water distribution system. Drinking water from a piped system is called tap water. Tap water is a public facility provided by the government in European cities but not in Indonesia. However, consumption of tap water by the academic community at UNS Surakarta was relatively low. This was caused by the trust and worthiness of UNS’s tap water. This research aimed to determine the quality of tap water at UNS Surakarta, both in aspects of physics, chemistry and microbiology. Selection of sampling points based on the location of the distribution pipes and sampling using the grab sampling method. The tap water analyzed by Indonesian National Standard (SNI) test for drinking water. The results were compared with the Indonesian Government Regulation on Drinking Water, namely PERMENKES 492/2010. The quality of physical, chemical and microbiological aspects of tap water at UNS Surakarta were meet Indonesian Government Regulations, namely Permenkes 492/2010. Thus, tap water from UNS Surakarta is equivalent to bottled drinking water and worth to drink.
Produksi Nata de Whey: Solusi Penanganan Limbah Keju dari PT. Mazaraat Lokanatura Indonesia dan Pemberdayaan Masyarakat Siti Lusi Arum Sari; Pangastuti Artini; Setyaningsih Ratna; Susilowati Ari; Purwoko Tjahjadi; Muhammad Fajar Permana
PRIMA: Journal of Community Empowering and Services Vol 9, No 2 (2025): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v9i2.80055

Abstract

Nata de Whey Production: Solutions for Handling Cheese Waste from PT. Mazaraat Lokanatura Indonesia and Community Empowerment. One of the problems faced by PT Mazaraat Lokanatura Indonesia is the abundant liquid cheese waste (whey). If not properly managed, cheese whey could be harmful to the  environment. Whey still contains a lot of milk nutrients so it can be used as a substrate for the production of nata de whey. Based on this, the purpose of this activity was to produced nata de whey from which was served as a nata-salak cocktail. This activity involves 2 partners, namely PT Mazaraat Lokanatura Indonesia and the Tegalrandu community, Srumbung, Magelang. The activity was carried out in an integrated manner with the KKN MBKM Biology Study Program, FMIPA UNS in Tegalrandu, Srumbung, Magelang. The stages of the activity were: socialization, provision of KKN students, training in making nata and cocktails, and mentoring. Training starts from whey preparation, fermentation, processing nata into cocktails, and packaging. Mentoring and monitoring activities were carried out directly by the KKN student group. Socialization and FGD with PT Mazaraat were held on June 26, 2023. Through this FGD, the management of PT. Mazaraat supports the use of whey by the community. Socialization with the Tegalrandu community were carried out in an integrated manner with the implementation of the MBKM KKN program. Before the training, KKN students had practiced making Nata and cocktails. The Tegalrandu village community was very enthusiastic about participating in the training activities for making nata de whey and nata-salak cocktails. Through this activity, A nata-salak cocktail product that may be utilized as an unique and extraordinary Tegalrandu village product was created. This activity provides an alternative solution to the waste problem of PT Mazaraat Lokanatura and opens up business opportunities for the Tegalrandu, Srumbung, Magelang community.
Isolation of Lactic Acid Bacteria from Soil and Its Antifungal Activity Against Aspergillus flavus Athiyyah Jihan Tifani; Tjahjadi Purwoko; Ari Susilowati
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.109898

Abstract

Food stocks, such as grains, fruits, and vegetables are vulnerable to spoilage by Aspergillus flavus, which typically controlled using chemical preservatives. Concerns about the health impact of these chemicals highlight the need for safer alternatives, such as biopreservatives. Lactic acid bacteria (LAB) are Generally Recognized as Safe (GRAS) microorganisms and have potential application as biopreservative agent, because LAB produced various metabolites with antifungal activities. LAB could be found in nutrient-rich environments, including rhizosphere soil and poultry house soil due to the nutrient residues for growth. The aims of this study were to isolate LAB from soils and to evaluate their antifungal activity with a focus on potential applications as biopreservatives. The LAB isolates from soil were characterized and screened for antifungal activity using the dual culture method. The LAB isolates inhibited A. flavus growth with the largest growth inhibition was showed by LAB isolate 1 that isolated from poultry house soil (17.65%). However, statistics analysis showed that fungal growth inhibition by LAB isolates were not significantly difference (p>0,05). Although the result was not statistically significant, all  LAB isolates significantly inhibited conidia development. This showed potential application of LAB isolates as biopreservative agent. Keywords: antifungal, Aspergillus flavus, biopreservative, food stock, lactic acid bacteria