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All Journal Health Research Journal of Indonesia Biodiversitas Journal of Biological Diversity
Siti Nur Azizah
Pharmacy Vocational Program, Politeknik Kesehatan Jember. Jl. Pangandaran No. 42, Jember 68121, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Eksplorasi Aktivitas Antibakteri Nanopartikel Emas dari Ekstrak Daun Katuk (Sauropus androgynus) terhadap Bakteri Gram Positif dan Gram Negatif Denok Risky Ayu Paramita; Hadi Barru Hakam Fajar Siddiq; Siti Nur Azizah
Health Research Journal of Indonesia Vol 4 No 5 (2026): Health Research Journal of Indonesia
Publisher : CV. Wadah Publikasi Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63004/hrji.v4i5.1199

Abstract

Meningkatnya kasus resistensi antibiotik mendorong pengembangan agen antibakteri alternatif berbasis nanoteknologi, salah satunya nanopartikel emas (AuNPs). Green synthesis merupakan metode sintesis AuNPs yang ramah lingkungan, aman, sederhana dan ekonomis dengan memanfaatkan ekstrak tumbuhan kaya senyawa bioaktif, seperti daun katuk (Sauropus androgynus). Penelitian ini bertujuan mengeksplorasi aktivitas antibakteri AuNPs hasil green synthesis dengan ekstrak daun katuk terhadap bakteri Gram positif dan Gram negatif. AuNPs selanjutnya diuji terhadap Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, dan Escherichia coli menggunakan metode difusi cakram pada konsentrasi 12,5%, 25%, 50%, dan 100%. Hasil penelitian menunjukkan AuNPs mampu menghambat pertumbuhan P. acnes, S. epidermidis, dan B. subtilis, namun tidak pada S. aureus maupun E. coli. Zona hambat terbesar pada S. epidermidis dengan konsentrasi 100% sebesar 2,04±0,62 mm, diikuti P. acnes dan B. subtilis masing-masing sebesar 1,10±0,90 mm dan 1,10±0,40 mm. Aktivitas antibakteri yang relatif lemah diduga berkaitan dengan waktu sintesis yang belum optimal sehingga reduksi ion emas belum berlangsung sempurna. Hal ini menyebabkan konsentrasi AuNPs yang dihasilkan masih rendah dan berdampak pada terbatasnya aktivitas antibakteri. Dengan demikian, AuNPs berbasis ekstrak daun katuk berpotensi sebagai agen antibakteri terhadap bakteri Gram positif, namun optimasi waktu sintesis diperlukan untuk meningkatkan hasil sintesis dan efektivitas antibakterinya.
Investigation of endophytic bacteria associated with Paederia foetida: Population, characterization, antibacterial, and pathogenicity analysis MELDA YUNITA; MORGAN OHIWAL; JUEN CARLA WARELLA; ELPIRA ASMIN; SITI NUR AZIZAH; PRESLI GLOVRIG SIAHAYA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 2 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260247

Abstract

Abstract. Yunita M, Ohiwal M, Warella JC, Azizah SN, Asmin E, Siahaya PG. 2025. Investigation of endophytic bacteria associated with Paederia foetida: Population, characterization, antibacterial, and pathogenicity analysis 26: 981-991. The aim of this study was to obtain non-pathogenic endophytic bacterial isolates that can be developed as antibacterials for further applications. Endophyte isolation was carried out in 4 different media to obtain diverse endophytes and characterized macroscopically and microscopically. Endophyte screening was done through antibacterial tests against 10 different pathogenic bacteria. Pathogenicity analysis was performed on Blood Agar Plate (BAP) to differentiate pathogenic and non-pathogenic group of endophytic bacteria. The results showed that Starch Casein Agar was the best media for isolation in terms of total population and morphological characteristics compared to the other three media. A total of 27 isolates were collected according to the different morphology. Screening through antibacterial tests revealed that 19 endophytic isolates were significantly able to inhibit various species of pathogenic bacteria (p-value of 0.001). Pathogenicity analysis showed that 8 of 19 isolates (HR1, HR2, HR3, K2, NL3, S2, A2, and A9) were categorized as ? hemolysis, while the other 11 isolates were categorized as ? hemolysis and ? hemolysis. The study concluded that 8 endophytic bacterial isolates associated with P. foetida leaves had the strong potential to be developed and can be applied in advanced antibacterial applications.
Co-Authors Denok Risky Ayu Paramita ELPIRA ASMIN Hadi Barru Hakam Fajar Siddiq JUEN CARLA WARELLA MELDA YUNITA MORGAN OHIWAL PRESLI GLOVRIG SIAHAYA