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Bacteria Analysis in the Ciwalen River Garut using Next Generation Sequencing of 16S Ribosomal RNA Tati Kristianti; Lida Amalia
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.10471

Abstract

The Ciwalen River flows through Garut, which is bordered by hundreds of leather tanning enterprises and community toilet waste. The purpose of this research is to analysis bacteria in Ciwalen river water using the Next Generation Sequencing (NGS) method of the 16s ribosomal RNA gene. The primers utilized are the V1-V9 primers for the 16s rRNA sequence, which will amplify the entire length of the rRNA gene and allow for species identification. The data revealed that there were 104,000 reads, 150,232,539 bases, and 81,761 bacterial species. Proteiniclasticum ruminis, Fusibacter ferrireducens, Fusibacter fontis, Fusibacter tunisiensis, Paludibacter propionicigenes, Arcobacter cloacae, Alishewanella jeotgali, Thiovirga sulfuroxydans, Pseudomonas fluvialis, and Thauera mechernichensis are the main bacterial species. In this study, another bacteria, Klebsiella pneumoniae, was also found which is known to degrade heavy metals, Cr, Pb and Cd. The profile of bacteria, as well as the presentation of their existence in this water sample, demonstrates the appropriateness of heavy metals from leather tannery waste in the Ciwalen river.
Macroalga Biodiversity at Karapyak Beach Pangandaran Fini Hidayanti; Tati Kristianti; Leni Sri Mulyani
Florea : Jurnal Biologi dan Pembelajarannya Vol. 13 No. 1 (2026)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v13i1.24191

Abstract

Karapyak Beach Pangandaran is area coast potentially rocky and sandy​ support growth macroalgae. However, information about diversity macroalgae in this region Still limited. This research aimed  to observe types and distribution of macroalgae on Karapyak Beach, Pangandaran. Study done use method survey descriptive with technique line and quadrant transects measuring 1 m × 1 m. Determination station observation done by purposive sampling as many as three stations in the intertidal zone. Identification macroalgae done based on characteristics thallus morphology in general macroscopic, including shape, color, and structure body. Research result show that macroalgae found​ consists of on three main divisions, namely Chlorophyta, Phaeophyta, and Rhodophyta, with a total of ten type. Identified type​ including Sargassum sp., Sargassum polycystum, Padina australis, Chaetomorpha crassa, Boergesenia forbesii, Ulva lactuca, Eucheuma cottonii, Gelidium robustum, Palmaria palmata, and Amphiroa beauvoisii. Distribution macroalgae influenced by factors environment like type substrate, intensity light, and tidal conditions.Study This provide initial data about diversity macroalgae on Karapyak Beach which can utilized as base management and conservation ecosystem coast.
Analisis Vegetasi Pohon dan Faktor Lingkungan menggunakan Metode Kuarter sebagai Sumber Belajar Biologi Friska Junita; Rifaatul Muthmainnah; Tati Kristianti
Jurnal Pendidikan Biologi Undiksha Vol. 13 No. 1 (2026)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v13i1.112087

Abstract

Keanekaragaman vegetasi pohon memiliki peran penting dalam menjaga keseimbangan ekosistem serta dipengaruhi oleh faktor lingkungan. Penelitian ini bertujuan untuk menganalisis struktur vegetasi pohon dan keterkaitannya dengan faktor lingkungan menggunakan metode kuarter sebagai dasar pengembangan sumber belajar biologi. Penelitian dilaksanakan di kawasan hutan Cagar Alam Pangandaran pada 30 Juni 2025 dengan 10 titik pengamatan. Data vegetasi dikumpulkan melalui pengukuran jarak pohon dan diameter batang setinggi dada (DBH), sedangkan faktor lingkungan yang diukur meliputi suhu udara, kelembaban udara, intensitas cahaya, pH tanah, kecepatan angin, dan jenis tanah. Data dianalisis menggunakan parameter kerapatan, frekuensi, dominansi, dan Indeks Nilai Penting (INP). Hasil penelitian menunjukkan terdapat 14 jenis pohon dengan spesies dominan Tectona grandis dan Syzygium lateata yang memiliki nilai INP tertinggi masing-masing sebesar 115,83 dan 72,58. Kondisi lingkungan berupa suhu 25,6–28 °C, kelembaban 77–80%, intensitas cahaya 1105–1850 lux, pH tanah 5,2, dan tanah liat mendukung dominasi kedua spesies tersebut. Hasil penelitian ini berpotensi dimanfaatkan sebagai sumber belajar biologi yang kontekstual pada materi keanekaragaman hayati dan ekologi tumbuhan.
The Molecular Identification of Bacteria in Plastic Waste at the Pasir Bajing Garut Landfill : plastic waste Tati Kristianti; Rifaatul Muthmainnah; Friska Junita; Iis Aisyah
Florea : Jurnal Biologi dan Pembelajarannya Vol. 13 No. 2 (2026)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v13i2.24635

Abstract

Plastic waste remains a major environmental concern worldwide. Approximately 418,262 tons of plastic waste, accounting for 20% of the total waste accumulation, are deposited annually at the Pasir Bajing Landfill (TPA) in Garut, Indonesia. Under these conditions, plastic serves both as a selective substrate for certain microorganisms and as a potential carbon source. This study aimed to identify bacteria associated with plastic waste at the Pasir Bajing Landfill using molecular techniques. Polystyrene waste samples were collected from three different sampling sites within the landfill area. Phylogenetic analysis based on 16S rRNA gene sequences revealed three bacterial isolates: isolate 2 was identified as Acinetobacter baumannii, isolate 5 as Atlantibacter hermannii, and isolate 6 as a member of the family Enterobacteriaceae. These isolates belong to the phylum Proteobacteria and may possess the potential to degrade plastic materials. Further studies are required to evaluate their plastic-degrading capabilities and to elucidate the metabolic pathways involved in the biodegradation process.
The Local Rice Varieties' Growth and Yield Reaction to Drought Conditions Dadang Sumardi; Popi Septiani; Husna Nugrahapraja; Chindy Ulima Zanetta; Tati Kristianti
Florea : Jurnal Biologi dan Pembelajarannya Vol. 13 No. 2 (2026)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v13i2.25018

Abstract

Local rice cultivars are traditionally developed and maintained through farmer-based agricultural practices. Identifying traits associated with drought tolerance in local rice germplasm is essential for the development of high-yielding, drought-resistant varieties. Therefore, this study aimed to evaluate the drought tolerance characteristics of local rice cultivars from West Java, Indonesia. Fifteen local rice cultivars were collected from three locations: Tanjungsari District (Sumedang Regency), Ciletuh District (Sukabumi Regency), and Pandeglang Regency. The experiment was arranged in a randomized block design with two treatment factors and three replications. The ten local rice cultivars evaluated exhibited different responses to variations in soil water content relative to field capacity. Based on the Drought Sensitivity Index (DSI), five cultivars were classified as sensitive, four as moderately tolerant, and one as tolerant to drought stress. Under optimal water availability, the Ampera, NN Banten, and Rancung cultivars demonstrated high grain yield potential. However, the Rancung cultivar was found to be susceptible to drought stress. In contrast, the Huma Hideung Ciletuh cultivar was classified as drought-tolerant because it showed only minor reductions in grain weight and grain number under drought conditions compared with optimal water availability.