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All Journal Techno.Com: Jurnal Teknologi Informasi BERNAS: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Pengabdian Masyarakat Panacea
Novian Adhipurna
Universitas Borneo Lestari
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Ilmiah Biologi UMA (JIBIOMA)

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Studi In Sillico Gen CTX-M15 sebagai Biomarker Escherichia coli ESBL Ayanti, Bio Putri; Adhipurna, Novian; Novita, Iswiyanti; Prihandini, Yustin Ari
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 2 (2024): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i2.5215

Abstract

Escherichia coli is most common nosocomial pathogenic bacteria recognized as the highest cause of ESBL production. Derivatives of CTX-M, especially type CTX-M-15 (blaCTX-M-15) as the dominant ESBL-encoding gene found among E. coli isolates. The most important component in PCR is primer. Primer design can be done by in silico bioinformatics analysis. This study was conducted to produce a primer sequence of E. coli CTX-M-15 gene as a candidate biomarker for E. coli ESBL detection. The method used was in sillico observational using the Primer3Plus application and in silico PCR on candidate primers for the E. coli CTX-M-15 gene. Primers were selected based on criteria including%GC, primer length, Tm (melting temperature), stability, and primer interactions (the presence of dimers and hairpins). The sample used was the nucleotide sequence of the E. coli CTX-M-15 gene strain K-20 plasmid Genbank: GQ330540.1. The results showed that 2 pairs of forward and reverse primer pairs (pairs 1 and pairs 5) were able to amplify the blaCTX-M-15 gene sequence measuring 525bp and 519bp. In silico PCR analysis resulted in 2 pairs of new primer pairs being successfully designed and potentially used as DNA biomarkers from Escherichia coli in early and rapid detection of ESBL infection.
Co-Authors Ayanti, Bio Putri Bio Putri Ayanti Cast Torizellia Mochammad Maulidie Alfiannor Saputera Novita, Iswiyanti Nurbidayah Prihandini, Yustin Ari Rahmiati Rahmiati Yustin Ari Prihandini