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All Journal Jurnal Kesmas dan Gizi (JKG) SEHATI: Jurnal Kesehatan Indonesian Journal of Community Empowerment Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)
Sa’adah Siregar
Institut Kesehatan Medistra Lubuk Pakam
Agriculture, Biological Sciences & Forestry Humanities Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Perbandingan Pertumbuhan Aspergillus flavus Pada Media Filtrat Bekatul Dengan Berbagai Variasi Konsentrasi Sa’adah Siregar; Vincentia Ade Rizky; Syukurin Juliani Gulo
SEHATI: Jurnal Kesehatan Vol 5, No 1 (2025)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/sehati.v5i1.79

Abstract

Mushrooms play a lot of role in life, both beneficial and detrimental. Certain types of fungi are capable of producing a toxic organic compound called mycotoxin. The mycotoxin produced by Aspergillus flavus is known as aflatoxin. The mushroom growth medium that is often used is SDA (Sabouraud Dextrose Agar). The composition of SDA media is peptone mycology, sugar, agar. The content of rice bran is carbohydrates, protein, water, and agar. The purpose of the study was to determine the comparison of growth diameter and effectiveness of Aspergillus flavus  concentration on bran filtrate media with various concentration variations. The method used in this study was experimental with inoculation of Aspergillus flavus fungi with a single dot method. The average colony diameter concentrations of 5%, 10%, 15% and 20% were 5.3 mm, 8.4 mm, 11.4 mm, and 16.5 mm. On SDA media  the average colony diameter is 17.6 mm. The concentration of bran filtrate media that is close to SDA  media is a concentration of 20%.  The results  of the One Way ANOVA  test showed a significant difference in the growth of Aspergillus flavus between various variations in bran filtrate concentration obtained a sig value of 0.000 less than 0.05. The conclusion of  data analysis using the One Way ANOVA  test was a significant difference in the growth of Aspergillus flavus between various variations in bran filtrate concentration obtained a sig value of 0.000 less than 0.05. In the bonfferoni test results, it was found that a concentration of 20% had similarities or significance to the positive control media of natural resources with a value of (p) 0.331 more than 0.05 which means that there is no significant difference between bran filtrate media at a concentration of 20% with positive control media  of natural resources
Analysis of the Effectiveness of Quality Management through the Implementation of Quality Assurance (QA) and Quality Control (QC) in Improving Microbiology Laboratory Examination Results at Grandmed Hospital Lubuk Pakam Rahmawati; Ahmad Hafizullah Ritonga; Sa’adah Siregar
JURNAL KESMAS DAN GIZI (JKG) Vol. 8 No. 2 (2026): Jurnal Kesmas dan Gizi (JKG)
Publisher : Fakultas Kesehatan Masyarakat Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/2g87nb78

Abstract

Microbiology laboratories play an important role in supporting diagnosis and clinical decision-making, so the quality of test results must be guaranteed. The complexity of the testing process requires the consistent application of quality management through Quality Assurance (QA) and Quality Control (QC) at all stages of pre-analytical, analytical, and post-analytical. This study aims to analyze the effectiveness of QA and QC implementation in improving microbiology laboratory test results at Grandmed Lubuk Pakam Hospital. This study uses a qualitative approach with a case study design. Data were collected through in-depth interviews, observations, and quality document reviews of the head of the laboratory, the doctor in charge, microbiology laboratory analysts, and administrative staff. Data analysis was conducted thematically to examine the QA and QC implementation process and factors affecting the effectiveness of quality management. The results showed that QA and QC had been implemented through SOPs, internal quality control, result validation, and quality documentation. However, their implementation has not been optimal due to limited human resources, overlapping tasks, and inconsistent evaluation and follow-up of quality audits. Nevertheless, the implementation of QA and QC has contributed positively to improving the reliability of test results and clinician confidence. The conclusion of this study is that the effectiveness of microbiology laboratory quality management through the implementation of QA and QC is influenced by the adequacy of human resources, management commitment, and consistency of implementation at the operational level
PENINGKATAN PENGETAHUAN MASYARAKAT TENTANG PENTINGNYA PEMERIKSAAN HBA1C DAN PENGENDALIAN TEKANAN DARAH PADA PASIEN DIABETES MELITUS MELALUI EDUKASI Sa’adah Siregar; Ika Nur Saputri
MAJU : Indonesian Journal of Community Empowerment Vol. 3 No. 4 (2026): MAJU : Indonesian Journal of Community Empowerment, Juli 2026
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v3i4.2809

Abstract

Diabetes mellitus is a chronic disease that is frequently accompanied by hypertension and may increase the risk of complications if not properly controlled. Limited public understanding regarding the importance of HbA1c testing and blood pressure management has become the basis for implementing this community service activity. This activity aimed to improve community knowledge about the importance of HbA1c examination as an indicator of glycemic control and blood pressure management among patients with diabetes mellitus. The methods included health education, counseling sessions, blood pressure measurements, and consultations on diabetes management based on research findings conducted. The results showed an improvement in participants’ understanding of the importance of regular HbA1c monitoring, blood pressure control, and the implementation of a healthy lifestyle to prevent diabetes-related complications. This activity is expected to enhance public awareness and encourage sustainable diabetes mellitus management
Comparison Of Potato (Solanum tuerosum L) and Corn (Zea mays) as Alternative Media To Nutrient Agar Media In The Groth of Staphylococcus aureus Bacteria Rahmawati; Sa’adah Siregar; Vincentia Ade Rizky
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2376

Abstract

Background: Microbiology is the study of microorganisms, which are microscopic organisms that can only be observed through a microscope. These microorganisms have an important role in various aspects of life, including in the health sector. Objective: This study aims to evaluate whether alternative media, such as potato and corn, can be used as a substitute for Nutrient Agar in supporting the growth of Staphylococcus aureus bacteria. Methods: This research is experimental, by comparing the effectiveness of potato and corn media as an alternative to Nutrient Agar for the growth of Staphylococcus aureus. Results: The results of the research in the One-way Anova test obtained a significant value (0.00) which means (p <0.05), so it can be concluded that there is a significant difference between the ability of Nutrient Agar media, potato media and corn media in growing Staphylococcus aureus bacteria. To find out each group that has a significant difference, the Post-hoc follow-up test is carried out. The results of the post-hoc follow-up test shown in Figure 4.2.2 obtained a significant value between Nutrient Agar media and potato media (0.00), Nutrient Agar media with corn media (0.558), and between potato and corn media (0.00). Conclusion: The results showed that potato media was significantly different in its ability to grow Staphylococcus aureus compared to Nutrient Agar and corn. Potato media shows potential as an alternative to Nutrient Agar, despite its different effectiveness.
Comparison of the Number of Staphylococcus aureus Colonies in Green Grass Jelly (Cyclea barbata Miers) and Black Grass Jelly (Mesona palustris) Sold in the Lubuk Pakam Area Selda manullang; Yemima Sundita Damanik; Sa’adah Siregar
Medistra Medical Journal (MMJ) Vol 3 No 2 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/aps49y92

Abstract

Food contamination by pathogenic bacteria is a public health problem that can cause digestive disorders and even serious infections. One of the bacteria commonly found in food is Staphylococcus aureus, which is capable of producing enterotoxins and causing food poisoning. Green grass jelly (Cyclea barbata Miers) and black grass jelly (Mesona palustris) are traditional food products widely consumed by the community and are generally produced and sold in a traditional manner, increasing the risk of bacterial contamination due to poor hygiene practices. This study aimed to compare the number of Staphylococcus aureus colonies in green grass jelly and black grass jelly sold in the Lubuk Pakam area. This research used a laboratory experimental method with a streaking technique on Mannitol Salt Agar (MSA) media using a sterile inoculating loop. Samples were inoculated onto the media and incubated to allow colony growth. Bacterial identification was carried out through macroscopic observation, Gram staining, and biochemical tests including catalase and coagulase tests. The results showed that all samples of green and black grass jelly were positive for Staphylococcus aureus. The total number of colonies in green grass jelly was 465 colonies with an average of 116.25 colonies, while black grass jelly showed a total of 880 colonies with an average of 220 colonies. The difference in the average colony count between the two samples was 103.75 colonies, indicating that black grass jelly had a higher level of contamination than green grass jelly. In conclusion, there is a difference
Real time polymerase chain reaction assay (RT-PCR) sebagai tes cepat mycobacterium tuberculosis dari sampel dahak pasien tuberculosis Visensius Krisdianilo; Vincentia Ade Rizky; Sa’adah Siregar; Asvia Rahayu
JOURNAL OF Medical Surgical Concerns Vol. 4 No. 1 (2024): June Edition 2024
Publisher : Published by: Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Himpunan Perawat Medikal Bedah Indonesia (HIPMEBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/msc.v4i1.371

Abstract

Background: Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. This bacteria can cause tuberculosis (TB). TB disease has been widely recognized as a cause of death which is quite high in the world. Tuberculosis causes the death of nearly one million women each year. Currently, no country in the world is free from tuberculosis. Data shows that Indonesia is the third largest contributor of tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria which is routinely carried out in hospitals or health centers uses microscopic diagnosis of acid-resistant bacilli (BTA). Recently, a rapid test of Mycobacterium tuberculosis was carried out using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method which targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extracting doxyribo nucleic acid (DNA) in a cartridge. The time needed for this diagnosis is approximately two hours until the results come out. Purpose: To see the results of the rapid test compared to the microscopic diagnosis at the Puskesmas. Method: Using the cross-sectional method, with a sample of 25 patients at the Lubuk Pakam Health Center. To detect Mycobacterium tuberculosis using the GeneXpert MTB/RIF tool. Furthermore, the data obtained was processed and examined using the Wilcoxon statistical test. Results: The value of the statistical test results using the Wilcoxon test showed that the p-value (Sig) was 0.264, the value was > 0.05, which means that in this study there were no significant differences in results between the microscopic method and RT-PCR. Conclusion: Overall there was no significant difference in the results between microscopy and RT-PCR methods. Keywords: Tuberculosis; Mycobacterium Tuberculosis; RT-PCR Pendahuluan: Mycobacterium tuberculosis dikenal sebagai bakteri yang sangat patogen. Bakteri ini berbentuk aerobik seperti batang dan memiliki ketahanan terhadap asam. Bakteri ini dapat menyebabkan tuberkulosis (TB). Penyakit TBC telah dikenal luas sebagai penyebab kematian yang cukup tinggi di dunia. Tuberkulosis menyebabkan kematian hampir satu juta wanita setiap tahun. Saat ini tidak ada satu negara pun di dunia yang bebas dari tuberkulosis. Data menunjukkan bahwa Indonesia merupakan penyumbang kasus tuberkulosis terbesar ketiga di dunia. Pemeriksaan bakteri mycobacterium tuberculosis yang rutin dilakukan di rumah sakit atau puskesmas menggunakan diagnosis mikroskopis acid-resistant basil (BTA). Baru-baru ini telah dilakukan uji cepat Mycobacterium tuberculosis menggunakan metode semi kuantitatif Real Time Polymerase Chain Reaction Assay (RT-PCR) yang menargetkan gen rpoB pada Mycobacterium tuberculosis, yang secara otomatis dapat memproses preparat dengan ekstraksi asam nukleat doxyribo (DNA) pada catridge. Waktu yang dibutuhkan dalam diagnosis ini adalah kurang lebih dua jam sampai hasilnya keluar. Tujuan: Untuk melihat hasil rapid test tersebut dibandingkan dengan diagnosis mikroskopis pada Puskesmas. Metode: Menggunakan metode  cross sectional, dengan sampel yaitu pasien Puskesmas Lubuk Pakam sebanyak 25 orang. Untuk mendeteksi Mycobacterium tuberculosis menggunakan alat GeneXpert MTB/RIF. Selanjutnya data yang didapat di olah dan diperiksa dengan menggunakan uji statistic Wilcoxon. Hasil: Nilai hasil uji statistic menggunakan uji Wilcoxon didapatkan hasil p-value (Sig) adalah 0.264 nilai tersebut > dari 0.05 yang berarti pada penelitian ini hasilnya tidak terdapat perbedaan hasil yang signifikan antara metode mikroskopis dengan RT-PCR. Simpulan: Secara keseluruhan tidak ada perbedaan yang signifikan dalam hasil antara metode mikroskopis dan RT-PCR.  
Co-Authors Asvia Rahayu Ika Nur Saputri Morischa Deyla Sitanggang Neti Eka Jayanti Rahmawati Selda manullang Syukurin Juliani Gulo Vincentia Ade Rizky Visensius Krisdianilo Yemima Sundita Damanik