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OPTIMIZING FIXATION DURATION FOR ENHANCED CLARITY IN Pediculus humanus-capitis WHOLE MOUNT PREPARATIONS USING 10% KOH AT 70°C Tika putri agustina; Musma Rukmana; Hasmiati; Fernando Andre Watung
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : LPPM Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.1041

Abstract

This study evaluates the optimal fixation duration using 10% potassium hydroxide (KOH) at 70°C for whole mount preparations of Pediculus humanus capitis (head lice). Accurate head lice identification is crucial for effective public health management. Specimens were fixed for 20, 25, 30, 35, and 40 minutes at 70°C, then processed through rinsing, acetic acid treatment, dehydration, and clearing before mounting for microscopic examination. Results showed that 25 minutes of fixation provided the clearest and most detailed preparations. This duration effectively achieved tissue dehydration and clarification without significant damage. Fixation for 20 minutes was insufficient, resulting in opaque specimens due to incomplete KOH diffusion. Conversely, 40 minutes of fixation caused over-fixation, leading to tissue damage and poorer color quality. The study underscores the importance of finding the right fixation duration. While shorter times are inadequate, longer durations can degrade specimen quality. A 25-minute fixation at 70°C is optimal for balancing clarity and structural preservation, improving head lice identification. This method enhances the quality of whole mount preparations and provides practical guidance for accurate and reliable ectoparasite examination in clinical and research settings.
Problem Based Learning sebagai Strategi Pembelajaran Kontekstual pada Materi Ekosistem: (Studi Tindakan Kelas di SMA Katolik St. Rosa de Lima Tondano) Limmey Maria Tambingon; Arrijani Arrijani; Musma Rukmana
Jurnal Cakrawala Pendidikan dan Biologi Vol. 2 No. 3 (2025): September: Jurnal Cakrawala Pendidikan dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jucapenbi.v2i3.690

Abstract

Biology education in high school has an important role in shaping students' science literacy and critical thinking skills. However, ecological material, particularly the topic of Ecosystem Components and their Interactions, is often considered difficult because it is abstract and not easy to observe directly. This research aims to improve student learning outcomes through the application of the Problem Based Learning (PBL) model, which emphasizes real problem-solving, collaborative discussion, and active involvement in the learning process. The research uses the design of Classroom Action Research (PTK) which was carried out in two cycles in class X of St. Rosa de Lima Catholic High School Tondano involving 10 students in the odd semester of the 2025/2026 school year. Data was collected through observation of teacher and student activities, learning outcome tests in the form of pretests and posttests, and learning documentation. The analysis was carried out descriptively, qualitatively, and quantitatively by calculating the average score, percentage of completion, and student involvement. The results of the study showed a significant increase. The percentage of teacher activity increased from 58.3% in the first cycle to 87.5% in the second cycle, while student activity increased from 45.83% to 83.33%. The average score of students also increased from 70% in cycle I to 90% in cycle II, with classical completeness reaching 90%. These findings confirm the effectiveness of PBL as a contextual Biology learning strategy, able to bridge ecological concepts with real life, and increase students' curiosity, cooperation, and critical thinking skills.
PENGEMBANGAN LABORATORIUM VIRTUAL BERBASIS TIKTOK PADA MATA KULIAH PRAKTIKUM KIMIA DASAR 1 UNTUK MENINGKATKAN KETERAMPILAN BERPIKIR KRITIS DAN PROSES SAINS Aisyiah Restutiningsih Putri Utami; Mustapa Mustapa; Chaleb Paul Manaari; Tika Putri Agustina; Musma Rukmana; Ermita Ermita
Jurnal Inovasi Pendidikan dan Sains Vol 5 No 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v5i1.1975

Abstract

The aim of this study was to develop a virtual laboratory TikTok-based. The virtual laboratory was designed for basic chemistry 1 labwork. The developed media was created to increase students' critical thinking and science process skills. The methodology of this research used ADDIE model. The virtual laboratory validations to evaluate the content and media were successfully done by two experts on each validation. Based on the validations, the score results were 93,6% for content and 88,1% for media. It means that the virtual laboratory was successfully created and is ready to be used as a teaching-learning source. The practical and effective tests were done in the chemistry education program grade 1 at A and B class. The data collection was through direct observation, interviews, and questionnaires. Next, the data was analyzed by descriptive statistic method. The research result showed that the Tiktok virtual laboratory was very good, practical, and effective to use in basic chemistry 1 labwork and could increase students' science process and critical thinking skills with an effectiveness percentage of 89,7%.