Article Per Year (5 Year)
p-Index From 2021 - 2026
0.408
P-Index
This Author published in this journals
All Journal Jurnal Inovasi Pendidikan dan Sains Bioma : Jurnal Ilmiah Biologi
Musma Rukmana
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Social Sciences

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
OPTIMIZING FIXATION DURATION FOR ENHANCED CLARITY IN Pediculus humanus-capitis WHOLE MOUNT PREPARATIONS USING 10% KOH AT 70°C Tika putri agustina; Musma Rukmana; Hasmiati; Fernando Andre Watung
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : LPPM Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.1041

Abstract

This study evaluates the optimal fixation duration using 10% potassium hydroxide (KOH) at 70°C for whole mount preparations of Pediculus humanus capitis (head lice). Accurate head lice identification is crucial for effective public health management. Specimens were fixed for 20, 25, 30, 35, and 40 minutes at 70°C, then processed through rinsing, acetic acid treatment, dehydration, and clearing before mounting for microscopic examination. Results showed that 25 minutes of fixation provided the clearest and most detailed preparations. This duration effectively achieved tissue dehydration and clarification without significant damage. Fixation for 20 minutes was insufficient, resulting in opaque specimens due to incomplete KOH diffusion. Conversely, 40 minutes of fixation caused over-fixation, leading to tissue damage and poorer color quality. The study underscores the importance of finding the right fixation duration. While shorter times are inadequate, longer durations can degrade specimen quality. A 25-minute fixation at 70°C is optimal for balancing clarity and structural preservation, improving head lice identification. This method enhances the quality of whole mount preparations and provides practical guidance for accurate and reliable ectoparasite examination in clinical and research settings.
PENGEMBANGAN LABORATORIUM VIRTUAL BERBASIS TIKTOK PADA MATA KULIAH PRAKTIKUM KIMIA DASAR 1 UNTUK MENINGKATKAN KETERAMPILAN BERPIKIR KRITIS DAN PROSES SAINS Aisyiah Restutiningsih Putri Utami; Mustapa Mustapa; Chaleb Paul Manaari; Tika Putri Agustina; Musma Rukmana; Ermita Ermita
Jurnal Inovasi Pendidikan dan Sains Vol 5 No 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v5i1.1975

Abstract

The aim of this study was to develop a virtual laboratory TikTok-based. The virtual laboratory was designed for basic chemistry 1 labwork. The developed media was created to increase students' critical thinking and science process skills. The methodology of this research used ADDIE model. The virtual laboratory validations to evaluate the content and media were successfully done by two experts on each validation. Based on the validations, the score results were 93,6% for content and 88,1% for media. It means that the virtual laboratory was successfully created and is ready to be used as a teaching-learning source. The practical and effective tests were done in the chemistry education program grade 1 at A and B class. The data collection was through direct observation, interviews, and questionnaires. Next, the data was analyzed by descriptive statistic method. The research result showed that the Tiktok virtual laboratory was very good, practical, and effective to use in basic chemistry 1 labwork and could increase students' science process and critical thinking skills with an effectiveness percentage of 89,7%.
Co-Authors Aisyiah Restutiningsih Putri Utami Chaleb Paul Maanari Ermita Ermita Fernando Andre Watung Hasmiati Mustapa Mustapa Tika Putri Agustina