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All Journal Berkala Kedokteran Journal of Social Science Homeostasis: Jurnal Mahasiswa Pendidikan Dokter
Wydiamala, Erida
Departement Mikrobiologi Dan Parasitologi Fakultas Kedokteran, Universitas Lambung Mangkurat Banjarmasin
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

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Efektivitas Ekstrak Etanol Daun Jeringau (Acorus calamus L.) sebagai Larvasida terhadap Larva Aedes aegypti Savitri Sita Nursanti Ali; Erida Wydiamala; Lisda Hayatie
Homeostasis Vol 4, No 1 (2021)
Publisher : Homeostasis

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Abstract: Dengue Hemorrhagic Fever (DBD) is a disease that can be transmitted through mosquito bites with a potential vector in the form of the Aedes aegypti mosquito. DHF transmission can be prevented by controlling the mosquito vector itself, one of which is the use of larvicides. However, the continuous use of larvicides can cause a resistant effect, so we need a natural larvicide that can be used by the community. This aim of this sttudy is to recogniize the effectiveness of the ethanol extract of jeringau leaves (Acorus calamus L) as a larvicide against Aedes aegypti larvae. This study is an experimental study with Posttest Only with Control Group Design and consists of 7 treatment groups: 5 extract concentrations, are 0,01%, 0,05%, 0,10%, 0,15% and 0,20%; negative control (pure water); positive control (temephos 1%). The treatments were exposed to third instar larvae for 24 hours with 4 repetitions. The result of the observation data were analyzed using the probit test and obtained LC50 value 0,035% and LC90 value 0,074%. Kruskal-Wallis test resultsed p = 0,000 and the Mann-Whitney test obtained p = 1,000, so there was no significant difference between the concentration of 0.01% with negative control and the concentration of 0,15% and 0.20% with positive control. The conclusion of this study is that the ethanol extract of jeringau leaves has larvacidal effectiveness against Ae. Aegypti larvae at a concentration of 0,05%, 0,10%, 0,15% and 0,20% with an LC50 value of 0,035% and LC90 of 0,074% and had a larvacidal effectiveness equivalent to 1% temefos at a concentration of 0.15% and 0.20%. Keywords: larvacide, jeringau leaves, Aedes aegypti Abstrak: Demam Berdarah Dengue (DBD) merupakan penyakit yang dapat menular melalui gigitan nyamuk dengan vektor potensialnya berupa nyamuk Aedes aegypti. Penularan DBD dapat dicegah dengan pengendalian vektor nyamuk itu sendiri, salah satunya adalah penggunaan larvasida. Namun, penggunaan larvasida yang terus-menerus dapat menyebabkan efek resisten sehingga diperlukan suatu larvasida alami yang dapat digunakan oleh masyarakat. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol daun jeringau (Acorus calamus L) sebagai larvasida terhadap larva Aedes aegypti. Penelitian ini merupakan studi eksperimental dengan Posttest Only with Control Group Design dan terdiri dari 7 kelompok perlakuan: 5 konsentrasi ekstrak yaitu 0,01%, 0,05%, 0,10%,0,15% dan 0,20%; kontrol negatif berupa air murni; kontrol positif berupa temefos 1%. Perlakuan dipaparkan terhadap larva instar III selama 24 jam dengan 4 kali pengulangan. Data hasil pengamatan dianalisis menggunakan uji probit dan didapatkan LC50 sebesar 0,035% dan LC90 sebesar 0,074%. Hasil uji Kruskal-Wallis didapatkan p=0,000 dan hasil uji Mann-Whitney didapatkan p=1,000, sehingga tidak terdapat perbedaan yang bermakna antara konsentrasi 0,01% dengan kontrol negatif serta konsentrasi 0,15% dan 0,20% dengan kontrol positif. Kesimpulan dari penelitian ini adalah ekstrak etanol daun jeringau memiliki efektivitas larvasida terhadap larva Ae. aegypti pada konsentrasi 0,05%, 0,10%, 0,15% dan 0,20% dengan nilai LC50 sebesar 0,035% serta LC90 sebesar 0,074%, dan memiliki efektivitas larvasida setara dengan temefos 1% pada konsentrasi 0,15% dan 0,20% . Kata-kata kunci: larvasida, daun jeringau, Aedes aegypti
Uji Aktivitas Ekstrak Etanol Daun Water Mimosa (Neptunia oleracea L) sebagai Larvasida terhadap Nyamuk Aedes aegypti Maisy Naqinie; Erida Wydiamala; Lisda Hayatie
Homeostasis Vol 4, No 3 (2021)
Publisher : Homeostasis

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Abstract

Abstract: Dengue hemorrhagic fever (DHF) is an infectious disease caused by the dengue virus which is transmitted by the Aedes aegypti. Transmission of DBD can be prevented by controlling vector of Aedes aegypti. The purpose of the research was to know how the larvicidal activity Water mimosa ethanol extract against Aedes aegypti. This research was experimental study using posstest-only with control group design method and used 7 treatment groups: 5 Larvicidal of extract concentration which where 0,01%, 0,1%, 1% and 3% negative control (aquadest); positive control (temephos 1%), with 4 replications. Each group was exposed to Aedes aegypti third instar larvae for 48  hours. Probit analysis showed that value of LC50 0,476 (0,340%-0,649%)and LC90 2,423 (1,468%-6,855% Kruskal-Wallis test result showed that p<0,05 and it was continued to Mann-Whitney test. The conclusion is of ethanol extract leaves Water mimosa has activity a larvicide against Aedes aegypti begun from concentration 0,03125% and that value LC50 and LC90 Water mimosa has the  LC50 0,476% (0,340%-0,649%) and LC90 2,423% (1,468%-6,855%) equal activity to temephos 1%. Keywords: water mimosa, Aedes aegypti, Larvicidal Abstrak: Penyakit Demam Berdarah Dengue adalah penyakit menular yang disebabkan oleh virus dengue yang ditularkan oleh vektor nyamuk Aedes aegypti. Penularan DBD dapat dicegah dengan pengendalian vektor nyamuk Aedes aegypti. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas larvasida ekstrak etanol Water mimosa terhadap larva Aedes aegypti. Penelitian ini bersifat eksperimental dengan metode post-test only with control group design dan menggunakan 7 kelompok perlakuan: 5 macam konsentrasi ekstrak yaitu 0,01%, 0,1%, 1%, dan 3% kontrol negatif (aquadest) dan kontrol positif (temefos 1%) dengan 4 kali pengulangan. Setiap kelompok dipaparkan terhadap larva instar III Aedes aegypti selama 48 jam dan jumlah kematiannya dihitung. Hasil uji probit didapatkan nilai LC50 sebesar 0,476 dan LC90 2,423. Hasil uji Kruskal-Wallis didapatkan p<0,05 dan dilanjutkan dengan uji Mann-Whitney. Kesimpulan penelitian ini adalah ekstrak etanol Water mimosa memiliki aktivitas larvasida terhadap larva Aedes aegypti pada konsentrasi 0,03125% dan nilai LC50 dan LC90 Water mimosa adalah LC50 0,476% (0,340%-0,649%) dan LC90 2,423% (1,468%-6,855%) Pada konsentrasi 0,5% dan1% memiliki aktivitas yang setara dengan temefos 1%. Kata-kata Kunci: Water mimosa, Aedes aegypti, Larvasida
Aktivitas Ekstrak Daun Kembang Bulan (Tithonia diversifolia) sebagai Ovisida dan Insect GROWTH REGULATOR terhadap Nyamuk Aedes aegypti Vina Salsabila; Agung Biworo; Erida Wydiamala
Homeostasis Vol 4, No 2 (2021)
Publisher : Homeostasis

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Abstract

Abstract: Kembang Bulan leaf are known to hold metabolic contents which are known to inhibit the growth of Aedes aegypti mosquito. The purpose of this study was to determine the activity of ovicides and insect growth regulators of Kembang Bulan leaf extract against Aedes aegypti. This study used a Completely Randomized Design method with 4 replications. The results of the probit analysis used for determination of lethal concentration values around 0.05%, 0.08%, 0.12%, 0.24%, and 0.45%. The result of the Mann-Whitney test showed that there is no concentration that has ovicide activity. However, the ethanol extract of kembang bulan leaf at concentration of 0.45% had ovicide activity of 48% better than temephos and pyriproxyfen. The result of insect growth regulator test showed that all lethal concentration had the same effect as pyriproxyfen. The conclusion is Kembang Bulan leaf have activity as ovicide and insect growth regulator against Aedes aegypti mosquito. Keyword: ovicides, insect growth regulator, kembang bulan leaves, Aedes aegypti. Abstrak: Daun kembang bulan diketahui memiliki kandungan metabolik yang berpotensi sebagai penghambat pertumbuhan nyamuk Aedes aegypti. Penelitian ini bertujuan untuk mengetahui aktivitas ovisida dan insect growth regulator ekstrak daun kembang bulan terhadap nyamuk Aedes aegypti. Penelitian ini menggunakan metode Rancangan Acak Lengkap dengan 4 kali replikasi. Hasil analisis probit digunakan untuk penetapan nilai konsentrasi letal sebesar 0,05%, 0,08%, 0,12%, 0,24%, dan 0,45%. Hasil uji Mann-Whitney menunjukkan bahwa tidak ada konsentrasi yang memiliki aktivitas ovisida. Namun, ekstrak etanol daun kembang bulan pada konsentrasi 0,45% memiliki aktivitas ovisida sebesar 48% lebih baik dari temefos dan pyriproxyfen. Hasil uji Insect Growth Regulator menunjukkan bahwa pada semua konsentrasi letal memiliki efek yang sama dengan pyriproxyfen. Kesimpulan dari penelitian ini adalah ekstrak etanol daun kembang bulan memiliki aktivitas sebagai ovisida dan insect growth regulator terhadap nyamuk Aedes aegypti. Kata-kata kunci: ovisida, insect growth regulator, daun kembang bulan, Aedes aegypti.
Efektivitas Ekstrak Etanol Daun Limau Kuit (Citrus hystrix DC) sebagai Repelen terhadap Nyamuk Aedes aegypti Hesty Tri Wira Drana Wasistha; Erida Wydiamala; Lisda Hayatie
Homeostasis Vol 5, No 2 (2022)
Publisher : Homeostasis

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Abstract

Abstract: Indonesia is one of the largest countries in the world with a tropical climate. The tropical climate may cause various tropical diseases such as Dengue Hemorrhagic Fever (DHF). The primary vector of DHF is the Aedes aegypti mosquito. The prevention of DHF can be done using repellent (mosquito repellent). Natural repellents are limau kuit leaves (Citrus hystrix DC). Limau kuit leaves contain secondary compounds, such as flavonoids, alkaloids, and tannins that have the potential as repellents. The study's general objective was to test the effectiveness of the ethanol extract of the limau kuit leaves extract against the Aedes aegypti mosquito. This research was a true experimental study with posttest only with control group design, consisting of 4 concentrations of ethanol extract of limau kuit leaves, namely 12.5%, 25%, 50%, 100%, DEET 15% (positive control), and aquadest (negative control) with four replications. Serial consentrations extract of limau kuit leaves, K(-) and K(+), were applied to the backs of mice exposed to mosquitoes for 5 minutes for every minute. The results showed that all concentrations were effective as repellents. The 100% concentration at every minute had no significant difference with the positive control (p>0.05). The conclusions from this research were etanol extact of limau kuit leaves with concentrations of 12.5%,25%,50%, and 100% was effective as a mosquito repellent Aedes aegypti, and ethanol extract of limau kuit leaves with a concentration of 100% was equivalent to 15% DEET. Keywords: Aedes aegypti, limau kuit leaves, repellent Abstrak: Indonesia merupakan salah satu negara terbesar di dunia yang memiliki iklim tropis. Iklim tropis menimbulkan berbagai macam penyakit tropis yang salah satunya dapat disebabkan oleh nyamuk, seperti Demam Berdarah Dengue (DBD). Vektor utama DBD adalah nyamuk Aedes aegypti. Pencegahan penyakit ini dapat dilaksanakan melalui beragam cara atau mekanisme, termasuk penggunaan repelen atau penolak nyamuk. Tanaman yang dapat digunakan sebagai repelen alami adalah daun limau kuit (Citrus hystrix DC). Daun limau kuit mengandung senyawa sekunder, seperti flavonoid, alkaloid, dan tanin yang berpotensi sebagai repelen. Tujuan umum penelitian adalah menguji efektivitas repelen ekstrak etanol daun limau kuit terhadap nyamuk Aedes aegypti. Penelitian ini merupakan penelitian eksperimental murni dengan posttest only with control grup design, terdiri dari 4 konsentrasi ekstrak etanol daun limau kuit yaitu 12,5%,25%,50%,100%, DEET15%(Kontrol positif) dan aquadest(Kontrol negatif) dengan pengulangan sebanyak 4 kali. Serial konsentrasi ekstrak etanol daun limau kuit, K(-) dan K(+) dioleskan ke punggung mencit yang dipaparkan nyamuk selama 5 menit setiap periode pengamatan pada semua menit. Konsentrasi 100% pada semua menit tidak memiliki perbedaan bermakna dengan kontrol positif(p>0,05). Simpulan dari hasil penelitian ini adalah ekstrak etanol daun limau kuit dengan konsentrasi 12,5%,25%,50%,dan 100% efektif sebagai repelen nyamuk Aedes aegypti dan ekstrak etanol daun limau kuit dengan konsentrasi 100% efektif sebagai repelen setara dengan DEET15%. Kata-Kata Kunci: Aedes aegypti, daun limau kuit, repelen
EFEKTIVITAS EKSTRAK ETANOL DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) SEBAGAI REPELEN TERHADAP NYAMUK Aedes aegypti Mayang Rustia Anggraini Putri; Erida Wydiamala; Lisda Hayatie
Homeostasis Vol 5, No 3 (2022)
Publisher : Homeostasis

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Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus and transmitted through the bite of the Aedes aegypti mosquito. One effective option to prevent the transmission of the disease is by avoiding the bite of the Aedes aegypti mosquito by using a repellent-type insecticide. The use of chemical insecticides widely circulated in the market often has negative effects, such as irritation to the skin. Red betel leaf contains several active compounds that may act as contact poisons and are effective as repellents. The purpose of this study was to test the effectiveness of ethanol extract of red betel leaf against Aedes aegypti mosquitoes. This study was an true experimental post-test only with a control group design, consisting of 4 concentrations of ethanolic extract of red betel leaf (12.5%, 25%, 50%, 100%), DEET 15% K(+), and aquades K(-) with four replications. Serial concentrations ethanol extract of red betel leaf K(+), and K(-) were applied to the back skin of mice exposed to mosquitoes for 5 minutes for every minute. The concentration of 100% at every minute had no significant difference with the positive control (p>0.05). The conclusions from this research were red betel leaf ethanol extract with concentrations of 12.5%, 25%, 50%, and 100% was effective as a mosquito repellent Aedes aegypti, and red betel leaf ethanol extract with a concentration of 100% was equivalent to 15% DEET
Analysis Factors Related To The Incidence Of Diarrhea In Toddlers In The Uptd Area Of The East Martapura Health Center, Banjar Regency Elisa Oktaviani Dewi Anggraeni; Husaini Husaini; Edi Hartoyo; Erida Wydiamala
Journal Of Social Science (JoSS) Vol 2 No 9 (2023): JOSS : Journal of Social Science
Publisher : Al-Makki Publisher

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Diarrhea is a disease that causes discharge of feces more than 3 times with a liquid consistency, which may be accompanied by blood or mucus, and the frequency is more frequent than normal (WHO, 2019). According to WHO and UNICEF, there are around 2 billion cases of diarrhea and 1.9 million children under five die from diarrhea worldwide every year. The 2018 Basic Health Research stated that the prevalence of diarrhea for all age groups was 8% and the prevalence rate for toddlers was 12.3%, while for infants, the prevalence of diarrhea was 10.6%. The aim of the study was to analyze the relationship between Hand Washing with Soap (CTPS), Latrine Ownership, Garbage Securing and Diarrhea in the UPTD of the East Martapura Health Center. The method used is Quantitative, the research was conducted using a Cross Sectional study design, with a total sample of 89. The instruments used were questionnaires and observation sheets. Dependent variable: Incidence of diarrhea in toddlers, independent variables: hand washing with soap, toilet ownership, and waste management. The results of the study showed that there was a relationship between washing hands with soap (p=0.000), toilet ownership (p=0.000) and the incidence of diarrhea in toddlers. There is no relationship between waste management (p=0.065) and the incidence of diarrhea in toddlers in East Martapura. The most influential variable on the incidence of diarrhea among toddlers in East Martapura was latrine ownership (p=0.000 Exp (B) = 10.496).
Co-Authors Adma Hayani Dona Yanti Agung Biworo Agus Yuwono Bagus, I Gede Dwi Prahesty Septheresia Enus Mebas Edi Hartoyo Elisa Oktaviani Dewi Anggraeni Hafizh Maulana Hesty Tri Wira Drana Wasistha Husaini Husaini Istiana Istiana Kenanga Marwan Sikumbang, Kenanga Marwan Lesiana, Rina Lia Yulia Budiarti Lisda Hayatie, Lisda Maisy Naqinie Mayang Rustia Anggraini Putri Melynda Lauren Nadila, Isfarani Raharjo, Kevin Prasetya Savitri Sita Nursanti Ali Sinta Anisa Susianto, Oky Vina Salsabila Zafira Aisyah Putri