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Daya Simpan Benih Botani Bawang Merah (Allium cepa var. agregatum) Setelah Invigorasi Qudus Sabha Adhinugraha; Eny Widajati; Endah Retno Palupi
Jurnal Agrotek Tropika Vol 12, No 4 (2024): JURNAL AGROTEK TROPIKA VOL 12, NOVEMBER 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i4.7715

Abstract

Benih botani bawang merah (TSS) merupakan alternatif bahan tanam dalam budidaya bawang merah.  Penggunaan TSS sebagai bahan tanam memiliki keunggulaan dibandingkan dengan benih asal umbi, antara lain kebutuhan benih yang lebih sedikit, dapat memutus siklus patogen, tidak ada masa dormansi, dan dapat disimpan dalam jangka waktu lebih dari satu tahun. Walaupun dapat disimpan dalam jangka waktu yang lama, namun TSS tetap akan mengalami deteriorasi atau kemunduran yang dapat menyebabkan penuruan mutu benih.   Salah satu upaya yang dapat dilakukan untuk meningkatkan kembali mutu benih adalah dengan perlakuan invigorasi KNO3.  Saat ini peran invigorasi terhadap daya simpan benih TSS masih belum diketahui.  Penelitian ini dilaksanakan Laboratorium Penyimpanan dan Pengujian Mutu Benih IPB University, Indonesia. Penelitian ini bertujuan untuk mengetahui informasi daya simpan TSS setelah invigorasi dan pengeringan.  Percobaan disusun menggunakan rancangan tersarang (nested design) dua faktor.  Faktor pertama adalah kondisi ruang simpan benih yang terdiri atas tiga taraf, yaitu suhu 28±2˚C, RH 73±7% (kondisi kamar); suhu 18±2 ˚C, RH 61±7% (kondisi ruang ber-AC); dan suhu 8±2˚C, RH 24±8% (kondisi lemari es). Faktor kedua adalah jenis kemasan, yang terdiri atas dua jenis, yaitu kemasan alumunium foil (ketebalan 0,08 mm) dan kemasan plastik polipropilen (PP) (ketebalan 0,06 mm). Faktor jenis kemasan tersarang pada faktor kondisi ruang simpan benih.    Hasil penelitian menunjukkan bahwa Mutu TSS pasca invigorasi dapat dipertahankan selama 16 MSS dalam ruang ber-AC (suhu 18±2 ˚C, RH 61±7%) dengan kemasan alumunium foil atau lemari es (suhu 8±2˚C, RH 24±8%) dengan kemasan alumunium foil atau plastik PP. 
PENINGKATAN PERTUMBUHAN DAN PRODUKSI JAGUNG UNGU VARIETAS JANTAN F1 MELALUI APLIKASI NUTRISI ZINC Adhinugraha, Qudus Sabha; Agustiansyah, Agustiansyah; Timotiwu, Paul Benyamin; Rahayu, Dwi
Fruitset Sains : Jurnal Pertanian Agroteknologi Vol. 13 No. 2 (2025): Juni: Ilmu Pertanian dan Bidang Terkait
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/fruitset.v13i2.6292

Abstract

Maize was one of the most important food crops in the world; therefore, the demand for corn continued to increase in line with the growing global population. In general, increasing maize productivity was achieved by fulfilling the requirements for macro and micronutrients, as well as ensuring supportive environmental conditions so that the plants could grow well. One of the essential micronutrients needed by plants was zinc. This study aimed to determine the effect of zinc nutrient application on the growth and yield of purple maize of the Jantan F1 variety, as well as to identify the optimal zinc application treatment to enhance the growth and production of this variety. This research was conducted from September 14, 2024 to November 23, 2024 at the Integrated Field Laboratory and Seed and Plant Breeding Laboratory, Faculty of Agriculture, University of Lampung. The research was conducted using a Randomized Complete Block Design (RCBD) consisting of five treatments with a single factor: P0 (control/no zinc application); P1 (seed priming with Zn 0.5%); P2 (seed priming with Zn 0.5% + foliar Zn 0.1% at 10, 20, and 30 DAP); P3 (seed priming with Zn 0.5% + foliar Zn 0.1% at 30, 40, and 50 DAP); and P4 (seed priming with Zn 0.5% + foliar Zn 0.1% at anthesis, 7 days after pollination (DAPn), and 14 DAPn). The experiment was conducted with four replications, resulting in 20 experimental units. The results showed that the application of zinc nutrients to the purple maize of the Jantan F1 variety improved plant growth, as reflected in plant height, number of leaves, leaf area, and chlorophyll content. In addition, zinc nutrient application also increased yield, as indicated by variables such as cob weight with husk, cob weight without husk, number of rows per cob, and number of kernels per cob. The best treatment was zinc priming 0.5% combined with foliar Zn 0.1% applied at the anthesis stage, 7 DAPn, and 14 DAPn.
Embriogenesis Somatik Kopi: Prinsip dan Keunggulannya : Review Adhinugraha, Qudus Sabha
Agriculture and Biological Technology Vol. 1 No. 2 (2024): Juni
Publisher : Science, Technology, and Education Care

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61761/agiotech.1.2.58-64

Abstract

Perbanyakan tanaman kopi (Coffea spp.) secara in vitro dapat menjadi solusi dalam permasalahan pemenuhan kebutuhan bibit kopi dalam skala besar serta dengan klon yang seragam dalam waktu singkat. Perlu diketahui lebih lanjut mengenai embriogenesis somatik pada kopi, serta perbandingan tanaman hasil embriogenesis somatik dengan tanaman hasil perbanyakan secara konvensional. Perbanyakan kopi melalui embriogenesis somatik secara in vitro lebih umum digunakan karena mampu menghasilkan bibit kopi dalam skala besar dengan biaya produksi yang lebih rendah. Sedangkan perbanyakan melalui pola regenerasi organogenesis melalui beberapa kesulitan seperti sulitnya strerilisasi eksplan, adanya dominansi apikal, serta laju multipikasi tunas yang rendah. Adapun tahap dari embriogenesis somatik terdiri atas pemilihan dan sterilisasi eksplan, induksi kalus, perbanyakan kalus, dan induksi kalus embriogenik. Berbagai sumber menyatakan bahwa pertumbuhan bibit kopi hasil dari embriosomatik lebih baik dibandingkan dengan penggunaan bibit hasil perbanyakan konvensional (asal biji).
Invigoration Increased Quality and Storability of True Seed of Shallot (Allium ascalonicum L.) Adhinugraha, Qudus Sabha; Widajati, Eny; Palupi, Endah Retno
Journal of Tropical Crop Science Vol. 9 No. 02 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.02.145-155

Abstract

Seed invigoration is a pre-planting seed treatment by balancing the seed water potential and stimulating their metabolic activities so that the seeds germinate simultaneously. In this study, invigoration treatments were applied to improve the quality of the true seeds of shallots (TSS) that had started to deteriorate. Invigoration increases the moisture content of the seeds so the seeds need to be re-dried to extend their storability. The aims of the study were to determine the best invigoration treatment and the drying method to maintain the benefit of invigoration, and to prolong TSS storability after invigoration and drying. This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia, and consisted of three experiments, i.e., invigoration of deteriorating TSS to improve their viability and vigor, drying after invigoration to extend seed storability, and seed storability after invigoration and drying treatments. The first experiment was arranged in completely randomized design with one-factor (invigoration solutions), i.e. control, medium containing 3% KNO3, 50 ppm GA3, 0.5 % ZnSO4 or PEG6000 -10 bar. The second experiment was conducted using a two-factor completely randomized design, drying temperature (20˚C, 30˚C, and 40˚C), and drying duration (8 hours, 16 hours, 32 hours, and 48 hours). The third experiment was arranged in a nested design with two factors. The first factor was a seed storage condition, i.e., ambient temperature, air-conditioned room, and refrigerator. The second factor was seed packages, i.e., aluminum foil and polypropylene plastic. Non-invigorated seeds were used as control. The studies were conducted on two shallot varieties separately, “Trisula” and “Lokananta”, except in the third experiment which only used “Lokananta”. The results showed that invigoration in 3 % KNO3 and 50 ppm GA3 effectively improved the vigor of deteriorating TSS. Drying the seeds at 40˚C for 8 hours was the most effective method to reduce seed moisture content without reducing the benefit of invigoration. Storing the invigorated TSS in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum foil or plastic packaging until 14 weeks maintained the benefit of invigoration. The quality of the invigorated TSS was maintained until 14 weeks in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum
Effect of seed priming on the germination of upland rice (Oryza sativa L.) sown in acidic soil AGUSTIANSYAH, AGUSTIANSYAH; TIMOTIWU, PAUL BENYAMIN; ADHINUGRAHA, QUDUS SABHA
Jurnal Natural Volume 25 Number 3, October 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i3.46127

Abstract

Upland rice is a type of rice that can be grown in Ultisol. The obstacles to cultivating plants in Ultisol are low pH and high aluminum content which hinders seed germination. Seed priming is a technology that can overcome problems with Ultisol. The aim of this research was to determine the effect of priming on the germination of upland rice seeds in acid soil conditions. The experiment conducted on non-factorial Completely Randomized Design (CRD) consisting of 8 treatments with 3 replications. The data were analyzed for variance and followed by Honest Significant Differences (HSD) at 5% using the Statistic R Program. Seed priming treatments tested were (1) Untreatment; (2) Hydropriming; (3) priming GA3 25 ppm; (4) priming GA3 50 ppm; (5) priming PEG 6000 10%; (6) priming PEG 6000 20%; (7) 0.5% KNO3 priming, and (8) 1% KNO3 priming. Rice seed are soaked in priming for 24 hours. The upland rice seeds of the Inpago 13 Fortiz variety were planted in Ultisol soil media with a pH of 4.45, Al content of 0.44%, and Fe 1.37%. The results showed that the priming treatment increased the germination and themost effective treatment was priming GA3 50 ppm, each value of showed germination (92.38%), germination speed (19.71% day-1), vigor index (83.81%), and time of appearance of plumule (2.96 day).