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All Journal International Journal of Advanced Health Science and Technology
Ratno Tri Utomo
Department of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia
Electrical & Electronics Engineering Health Professions Nursing Public Health

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Difference in quality of preparates of mice kidney histology using xylol and sunflower oil (Helianthus annuus) on deparafinization processes Adin Bhagawanti Poernomo; Juliana Christyaningsih; Ratno Tri Utomo; Wisnu Istanto
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.512

Abstract

ematoxylin-Eosin staining begins with deparaffinization. Deparaffinization aims to remove the remaining paraffin in the tissue and xylol is a commonly used deparaffinization material but the use of xylol has a bad impact on health and also the environment because of its toxic nature besides the price of xylol is relatively more expensive. A more natural material that is not harmful to the body and the environment is needed as an alternative to xylol. Sunflower oil is a type of vegetable oil that contains oleic acid. The oleic acid content in sunflower oil has a chain of carbon atoms that is non-polar and water insoluble so that it can dissolve paraffin which is non-polar and hydrophobic. This study was conducted at Faculty of Veterinary Medicine Airlangga University, Veterinary Farm Center and the Cytohistotechnology Laboratory of the Medical Laboratory Technology Department of the Surabaya Ministry of Health Polytechnic also this study was conducted from January - May 2025. which examined the differences in the quality of mice kidney histology preparations using xylol and sunflower oil in the deparaffinization process with experimental research methods and the sample size obtained using the federer formula was 24 preparations and divided into 4 groups including xylol, sunflower oil at 50oC for 5 minutes, 10 minutes and 15 minutes. The data were statistically processed with the Kruskal Wallis non-parametric test and the results showed that Ho was accepted (P = 0.350) so it can be concluded that there is no significant difference between the preparations deparafinitation with sunflower oil and xylol in hematoxylin eosin
Comparison Of Fixation Using NBF 10% and Honey 13% with Time Variation On Mice Heart Histology Preparations Using Hematoxylin Eosin Staining Amirah Putri Zerlina; Juliana Christyaningsih; Ratno Tri Utomo; Suliati Suliati
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.515

Abstract

The fixation stage is the initial stage of making histopathology preparations whose role is to maintain the cell structure of the tissue so that it is like living tissue and prevent tissue damage, thus facilitating the process of macroscopic and microscopic analysis. The fixative solution that is often used for standard preservation in anatomical pathology laboratory examinations is Neutral Buffered Formalin (NBF) 10%, which has the advantage of a pH that is close to neutral and effective in fixing soft tissue for 12-24 hours. However, NBF 10% is carcinogenic and has high toxicity. There is a natural fixative solution, honey, which contains fructose and glucose and has an acidic pH that has antimicrobial, antioxidant and antiautolysis properties. In addition, honey has also been widely used as a preservative because it is easily available, affordable, and without toxicity effects. The purpose of this study was to determine the comparison of the quality of histology preparations of mice heart tissue fixed with 10% NBF and 13% honey with time variations. This research is a type of laboratory experimental research conducted in November 2024-April 2025. The sample used is the heart of healthy male mice. The number of sample preparations used was 27 sample preparations. Data analysis through scoring and statistically processed using non-parametric Kruskal-Walis test, if there is a difference followed by Mann-Whitney test. The results showed that fixation with 13% honey solution with a time variation of 24 hours has the same comparison of preparation quality results with 10% NBF solution for 24 hours. Based on the Mann-Whitney test, the result is 0.018 (p < 0.05) for 13% honey solution for 12 hours and 0.634 (p > 0.05) for 13% honey solution for 24 hours. It can be concluded that fixation with 13% honey solution for 24 hours is effective as an alternative fixation solution with natural ingredients that are safe in fixing tissues
Co-Authors Adin Bhagawanti Poernomo Amirah Putri Zerlina Juliana Christyaningsih Suliati Suliati Wisnu Istanto