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All Journal Journal Of Vocational Health Studies Jurnal Kesehatan Poltekita : Jurnal Ilmu Kesehatan CENDEKIA : Jurnal Ilmu Pengetahuan International Journal of Advanced Health Science and Technology
Ratno Tri Utomo
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya,
Biochemistry, Genetics & Molecular Biology Education Electrical & Electronics Engineering Health Professions Medicine & Pharmacology Nursing Public Health

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Antidiabetic Effect of Noni Fruit and Moringa Leaves Extract on Blood Glucose Level, Hepatic and Pancreatic Histological Features of Diabetic Mice Utomo, Ratno Tri; Christyaningsih, Juliana; Rahmawati, Hanifa; Meidayana, Ema; Wulandari, Meisa; Oktavianingsih, Yulis; Andriyani, Nurul
Jurnal Kesehatan Vol 14 No 1 (2023): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v14i1.3619

Abstract

One of the chronic diseases with high blood glucose levels is a characteristic of Diabetes Mellitus. Utilization of herbal ingredients needs to be developed to reduce blood sugar levels in diabetes, and one of them is by using moringa leaf extract and noni fruit. This study aimed to analyze the effect of a combination of moringa leaf extract and noni fruit on blood glucose levels and tissue cytology in diabetic mice. The study was a randomized pretest-posttest control group design using 28 male mice divided into seven groups. The data analysis showed the lowest decrease in blood glucose levels in the group of diabetic mice given noni fruit and moringa leaf extract at a dose of 150mg/20g BW/oral/day for two weeks. The results of histological examination of liver and pancreas tissue showed no tissue is damaged.
Characterization of DNA Quality, TPOX Locus, TH01 Locus in Seawater Soaked Nails in Forensic Identification Utomo, Ratno Tri; Endarini, Lully Hanni; Yudianto, Ahmad; Christyaningsih, Juliana
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 3 (2023): November
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i3.3028

Abstract

Forensic science is multiplying. One of them is the identification of events at sea. Of course, the victim’s body can be submerged in seawater for days so that components in seawater can affect the victim’s body. This research aimed to see the effect of storage time on the level and purity of DNA in nail samples. Identification of nail DNA using the Polymerase Chain Reaction (PCR) method. The DNA purity test examines nail samples using a spectrophotometer read at 260 and 280 nm wavelengths. Measurement of DNA levels using the PCR instrument on the STR TPOX locus, the result was 32.02 ug/uL on positive control, 36.73 ug/uL in seawater immersion on the 2nd day, 35.35 ug/uL in seawater immersion on the 7th day, and 34.5 ug/uL in seawater immersion on the 20th day. At the STR TH01 locus, the result was 32.155 ug/uL on positive control, 38.05 ug/uL on the 2nd day of immersion in seawater, 35.18 ug/uL on the 7th day, and 33.88 ug/uL on the 20th day. Results Examination of the Total Plate Number of seawater on the 2nd day, 7th day, and 20th day of immersion found no colonies of germ colony growth. This study obtained sufficient DNA purity from the 2nd day, 7th day and 20th day. So that the samples could be analyzed for DNA levels, the result obtained from the study showed a decrease in DNA levels at the TPOX Locus and TH01 Locus from the 2nd day, 7th day, and 20th day.
Difference in quality of preparates of mice kidney histology using xylol and sunflower oil (Helianthus annuus) on deparafinization processes Adin Bhagawanti Poernomo; Juliana Christyaningsih; Ratno Tri Utomo; Wisnu Istanto
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.512

Abstract

ematoxylin-Eosin staining begins with deparaffinization. Deparaffinization aims to remove the remaining paraffin in the tissue and xylol is a commonly used deparaffinization material but the use of xylol has a bad impact on health and also the environment because of its toxic nature besides the price of xylol is relatively more expensive. A more natural material that is not harmful to the body and the environment is needed as an alternative to xylol. Sunflower oil is a type of vegetable oil that contains oleic acid. The oleic acid content in sunflower oil has a chain of carbon atoms that is non-polar and water insoluble so that it can dissolve paraffin which is non-polar and hydrophobic. This study was conducted at Faculty of Veterinary Medicine Airlangga University, Veterinary Farm Center and the Cytohistotechnology Laboratory of the Medical Laboratory Technology Department of the Surabaya Ministry of Health Polytechnic also this study was conducted from January - May 2025. which examined the differences in the quality of mice kidney histology preparations using xylol and sunflower oil in the deparaffinization process with experimental research methods and the sample size obtained using the federer formula was 24 preparations and divided into 4 groups including xylol, sunflower oil at 50oC for 5 minutes, 10 minutes and 15 minutes. The data were statistically processed with the Kruskal Wallis non-parametric test and the results showed that Ho was accepted (P = 0.350) so it can be concluded that there is no significant difference between the preparations deparafinitation with sunflower oil and xylol in hematoxylin eosin
Comparison Of Fixation Using NBF 10% and Honey 13% with Time Variation On Mice Heart Histology Preparations Using Hematoxylin Eosin Staining Amirah Putri Zerlina; Juliana Christyaningsih; Ratno Tri Utomo; Suliati Suliati
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.515

Abstract

The fixation stage is the initial stage of making histopathology preparations whose role is to maintain the cell structure of the tissue so that it is like living tissue and prevent tissue damage, thus facilitating the process of macroscopic and microscopic analysis. The fixative solution that is often used for standard preservation in anatomical pathology laboratory examinations is Neutral Buffered Formalin (NBF) 10%, which has the advantage of a pH that is close to neutral and effective in fixing soft tissue for 12-24 hours. However, NBF 10% is carcinogenic and has high toxicity. There is a natural fixative solution, honey, which contains fructose and glucose and has an acidic pH that has antimicrobial, antioxidant and antiautolysis properties. In addition, honey has also been widely used as a preservative because it is easily available, affordable, and without toxicity effects. The purpose of this study was to determine the comparison of the quality of histology preparations of mice heart tissue fixed with 10% NBF and 13% honey with time variations. This research is a type of laboratory experimental research conducted in November 2024-April 2025. The sample used is the heart of healthy male mice. The number of sample preparations used was 27 sample preparations. Data analysis through scoring and statistically processed using non-parametric Kruskal-Walis test, if there is a difference followed by Mann-Whitney test. The results showed that fixation with 13% honey solution with a time variation of 24 hours has the same comparison of preparation quality results with 10% NBF solution for 24 hours. Based on the Mann-Whitney test, the result is 0.018 (p < 0.05) for 13% honey solution for 12 hours and 0.634 (p > 0.05) for 13% honey solution for 24 hours. It can be concluded that fixation with 13% honey solution for 24 hours is effective as an alternative fixation solution with natural ingredients that are safe in fixing tissues
CORRELATION OF NEUTROPHIL/LYMPHOCYTE RATIO (NLR) AND HIGH SENSITIVITY TROPONIN-I (hs-TnI) VALUES IN ACUTE HEART DISEASE PATIENTS Dwi Rahayu; Evy Diah Woelansari; Museyaroh; Ratno Tri Utomo
Journal of Vocational Health Studies Vol. 10 No. 1 (2026): July 2026 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V10.I1.2026.9-14

Abstract

Background: Acute heart disease is among the ten most common non-communicable diseases in Indonesia. Diagnosis of acute heart disease is commonly confirmed using electrocardiography (ECG) or troponin measurement; however, these diagnostic tools are not available in all healthcare facilities. The Neutrophil-to-Lymphocyte Ratio (NLR) is a readily accessible laboratory parameter that can be assessed in patients presenting with chest pain. Purpose: This study aimed to determine the relationship between NLR and High-Sensitivity Troponin I (hs-TnI) levels in patients with acute heart disease who need rapid treatment. Method: This descriptive quantitative study used secondary data obtained from Husada Utama Hospital, Surabaya, covering the period from January 2023 to March 2024. A total of 52 patient records that met the inclusion criteria were analyzed. Statistical analysis was performed using the Spearman correlation test. Result: The findings of this study demonstrated a positive correlation between NLR and hs-TnI levels in patients with acute heart disease at RSUD Husada Utama Surabaya, indicating that an increase in troponin I levels was accompanied by an increase in NLR levels. The majority of patients with acute heart disease are 16 men with a range of ages 46 - 55 years. Data analysis using the Spearman test obtained a correlation coefficient of 0.279, indicating the level of strength of the relationship between variables and a significance value of 0.045 <0.05. Conclusion: There is a significant correlation between NLR and hs-TnI levels in acute heart disease at Husada Utama Hospital, Surabaya.
Comparison of 10% Sucrose Fixative and 10% NBF Fixative on Hepatic Histology of Mice Using He Staining at Variable Duration Gabriella Jihan Fatma; Juliana Cristyaningsih; Ratno Tri Utomo; Evy Dyah Woelansari
International Journal of Advanced Health Science and Technology Vol. 6 No. 3 (2026): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v6i3.526

Abstract

NBF 10% is the most commonly used fixation solution in histopathological examinations, because this solution can maintain tissue quality as its initial state. The results of preparations from tissues fixed using 10% NBF have good quality when microscopic observation is carried out. However, routine exposure to NBF can have negative effects such as eye, nose, and throat disorders, NBF is also carcinogenic as stated by the Occupational Safety and Health Administration (OSHA). So, it is necessary to need an alternative to 10% NBF as a fixation substance to minimize the adverse effects. Based on research that has been done, it can be stated that natural sweeteners have the potential to be an alternative fixation solution. This study aims to evaluate 10% sucrose as an alternative to 10% NBF for hepatic tissue fixation. Histological quality was assessed based on the staining of the cell nucleus, cytoplasm and uniform color in the preparation. The type of research used was experimental. This research was conducted at the Farma Veterinary Center as a validation site and test animal provider, the Cytohistotechnology Laboratory of the Department of Medical Laboratory Technology as a research site, and the Faculty of Veterinary Medicine Unair as a place for excision of mice. The test material used was mice hepar with 6 replications per treatment group. After observation, the data were processed using Kruskall-Wallis and Mann-Whitney in the 10% sucrose group for 12 hours and 24 hours showed comparable results with 10% NBF for 24 hours because based on the mann whitney test, the value was obtained (p>0.05). At 10% sucrose for 6 hours showed results that were not comparable to 10% NBF for 24 hours because the man whitney test obtained a value of (p <0.05). So that 10% sucrose shows its potential as a safer alternative.
GAMBARAN HASIL PEMERIKSAAN HEPATITIS B SURFACE ANTIGEN DAN ANTIBODI HEPATITIS C VIRUS PADA IBU HAMIL Vela Navira Rezi; Evy Diah Woelansari; Ratno Tri Utomo
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 6 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v6i4.14316

Abstract

Hepatitis B and Hepatitis C remain public health problems that may cause serious complications in pregnant women and fetuses and can also be transmitted vertically from mother to baby. Early detection through laboratory examination during pregnancy is essential as an effort to prevent transmission and provide optimal management. This study aimed to determine the results of Hepatitis B Surface Antigen (HBsAg) and Hepatitis C Virus Antibody (Anti-HCV) examinations among pregnant women at Manding Public Health Center, Sumenep Regency. The study sample consisted of 32 pregnant women in the first and second trimesters who underwent laboratory examinations at Manding Public Health Center. The samples were selected using a purposive sampling technique based on the inclusion and exclusion criteria. Primary data were obtained from blood plasma samples examined using the immunochromatographic method (rapid test) according to standard operating procedures. Data were analyzed using descriptive methods to describe the frequency distribution of reactive and non-reactive results for HBsAg and Anti-HCV testing. This study was conducted at the Laboratory of Manding Public Health Center, Sumenep Regency, from February 1–28, 2026. The results showed that among 32 respondents, all obtained non-reactive HBsAg results (100%) and all Anti-HCV results were also non-reactive (100%). ABSTRAK Hepatitis B dan Hepatitis C masih menjadi masalah kesehatan masyarakat yang berpotensi menyebabkan komplikasi serius pada ibu hamil maupun janin, serta dapat ditularkan secara vertikal dari ibu ke bayi. Deteksi dini melalui pemeriksaan laboratorium pada masa kehamilan sangat penting dilakukan sebagai upaya pencegahan penularan serta penanganan yang optimal. Penelitian bertujuan untuk mengetahui gambaran hasil pemeriksaan Hepatitis B Surface Antigen (HBsAg) dan Antibodi Hepatitis C Virus (Anti-HCV) pada ibu hamil di Puskesmas Manding, Kabupaten Sumenep. Sampel penelitian terdiri dari 32 ibu hamil trimester I dan II yang menjalani pemeriksaan laboratorium di Puskesmas Manding, pengambilan sampel dilakukan menggunakan teknik purposive sampling berdasarkan kriteria inklusi dan ekslusi. Data primer diperoleh dari plasma darah yang diperiksa menggunakan metode immunokromatografi (rapid test) sesuai standar operasional prosedur. Analisis data dilakukan menggunakan metode deskriptif untuk menggambarkan distribusi frekuensi hasil  reaktif dan non-reaktif pada pemeriksaan HBsAg dan Anti-HCV. Penelitian dilaksanakan di Laboratorium Puskesmas Manding Kabupaten Sumenep pada periode 1-28 Februari 2026. Hasil menunjukkan bahwa dari 32 responden, seluruhnya mendapatkan hasil HBsAg non-reaktif (100%) dan hasil Anti-HCV non-reaktif (100%).
Co-Authors Adin Bhagawanti Poernomo Ahmad Yudianto Amirah Putri Zerlina Andriyani, Nurul Dwi Rahayu Endarini, Lully Hanni Evy Diah Woelansari Evy Dyah Woelansari Gabriella Jihan Fatma HANIFA RAHMAWATI Istanto, Wisnu Juliana Christyaningsih Juliana Cristyaningsih Meidayana, Ema Museyaroh, Museyaroh Oktavianingsih, Yulis Suliati Suliati Vela Navira Rezi Wulandari, Meisa