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All Journal Jurnal Penelitian Kesehatan Suara Forikes Manuju : Malahayati Nursing Journal Anglophile Journal International Journal of Advanced Health Science and Technology Jurnal Pengabdian Kepada Masyarakat Mulawarman Jurnal Pengabdian Kesehatan Beguai Jejama
Wisnu Istanto
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia
Education Electrical & Electronics Engineering Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Deteksi Gen Penyandi Resistensi Insektisida Karbamat (Ace-1) pada Nyamuk Aedes Aegypti Metode Pcr Revi Zakiyatul Maftukhah; Retno Sasongkowati; Wisnu Istanto; Anita Dwi Anggraini
Malahayati Nursing Journal Vol 4, No 10 (2022): Volume 4 Nomor 10 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v4i10.7571

Abstract

ABSTRACT Dengue Hemorrhagic Fever (DHF) is a high endemic problems, especially in the tropics. Effort to prepare for the surge in cases, it is necessary to develop in molecular level, detection of the Ace-1 gene can be used as an important indication of the resistance Aedes aegypti to carbamate insecticides. Purpose to detect the presence or absence of the Ace-1 gene of Aedes aegypti against carbamate insecticides using the PCR method. Methods descriptive quantitative analytic method of observational data. Treatments in experiment consisted of four test bottles and one control bottle. The results that appear on the detection of Ace-1 gene using RT-PCR are CT values. Results showed that from  4 existing samples, 2 of them were positive with a value, sample 1 (A01) had a CT value of 1.00, sample 4 (A04) had a CT value of 4.42 while other 2 were negative which was indicated by the emergence of N/A which means Not Available or not available primary gene in target gene. Conclusion percentage obtained was 50% of samples with Ace-1 gene detected and 50% of other samples not detecting Ace-1 gene. Keywords: Aedes aegypti, Ace-1, CT value  ABSTRAK Demam Berdarah Dengue (DBD) menjadi salah satu masalah endemis yang cukup tinggi khususnya di daerah tropis. Sebagai upaya kesiapsiagaan terhadap lonjakan kasus yang terjadi perlu dikembangkan ke tingkat molekuler yaitu deteksi gen Ace-1 yang dapat dijadikan sebagai indikasi penting dari resistensi nyamuk Aedes aegypti terhadap jenis insektisida golongan karbamat. Tujuan untuk mendeteksi ada tidaknya gen Ace-1 nyamuk Aedes aegypti terhadap insektisida karbamat menggunakan metode PCR. Metode Penelitian deskriptif kuantitatif metode analitik data observasi. Banyaknya perlakuan dari penelitian ini terdiri dari empat botol uji dan satu botol kontrol. Hasil yang muncul pada deteksi gen Ace-1 menggunakan RT-PCR berupa nilai CT. Hasil menunjukkan dari 4 sampel yang ada diperoleh hasil 2 diantaranya positif dengan nilai yaitu pada sampel 1 (A01) mempunyai nilai CT sebesar 1,00, pada sampel 4 (A04) mempunyai nilai CT sebesar 4,42 sedangkan 2 lainnya negatif yang ditandai dengan munculnya N/A yang berarti Not Available atau tidak tersedia gen primer pada gen target. Kesimpulan presentase yang diperoleh sebesar 50% sampel yang terdeteksi adanya gen Ace-1 dan 50% sampel lainnya tidak terdeteksi adanya gen Ace-. Kata kunci: Aedes aegypti, Ace-1, nilai CT
Validation and Reliability of Self-Efficacy English Learning of Health Vocational Students with Jigsaw Learning Strategy Wisnu Istanto; Noer Amelia; Sapto Dwi Anggoro
Anglophile Journal Vol 3 No 1 (2023): Anglophile Journal
Publisher : CV. Creative Tugu Pena

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51278/anglophile.v3i1.481

Abstract

The purpose of this study was to put to the test a self-efficacy survey of four language skills (listening, speaking, writing, and reading) using the Jigsaw Learning Strategy. This study was a descriptive qualitative study in which 20 question items about self-efficacy and four language skills in English mastery for health vocational students are tested. This qualitative study was carried out in two classrooms with 39 health vocational students each from January 2021 to August 2022. Two classrooms were set up for the lecture group, one for Jigsaw and one for non-Jigsaw. Self-efficacy questionnaires were used to gather information. In the learning group, there were fourteen sessions totaling two hours, during which each class learned the same practice and comprehension material. Four language skills were worked on by the students. The questionnaire data was analyzed by the product moment to determine validity, and all were found to be valid with results greater than the value of the r table and less than 0.025. The reliability test results on questionnaire data revealed that the data were reliable or consistent. The Keiser-Meyers-Oklin Self-Efficacy Measure in the group of questions for all questionnaire items could be considered appropriate. The Measure of Sampling Adequacy results were also used to determine whether the self-efficacy variable was large enough for further analysis. The communality value was greater than 0.03, indicating a positive relationship between variables. Descriptive analysis, jigsaw classes respond better to self-efficacy surveys than non-jigsaw classes, implying increased self-confidence in the English learning process. Keywords: Jigsaw Learning Strategy, Self-Efficacy English Learning, Validation and Reliability
Peningkatan Kemandirian Siswa dalam Menerapkan Pola Hidup Bersih dan Sehat untuk Mencegah dan Mengurangi Infeksi Soil Transmitted Helminths (Sth) di SD MI Sunan Ampel 1 Sidorogo-Trosobo Kecamatan Taman Kabupaten Sidoarjo Suliati; Wisnu Istanto; Anita Dwi Anggraini
ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN
Publisher : Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Soil Transmitted Helminths (STH) adalah golongan cacing usus (Nematoda Usus) dalam perkembanganya membutuhkan tanah untuk men jadi bentuk infektif. Golongan STH yang habitatnya pada usus manusia adalah Ascaris lumbricoides, Hookworm (Necator americanus dan Ancylostoma duodenale), Strongiloides stercoralis, Trichuris trichiura. Madrasah Ibtidaiyah Sunan Ampel II mulai kiprahnya dalam dunia pendidikan sejak tahun1988 atas prakarsa beberapa tokoh agama, tokoh masyar akat dan adanya tuntutan dari masyarakat setempat. Mengingat semakin banyaknya output yang dihasilkan. Madrasah Ibtidaiyah Sunan Ampel II setiap tahunnya, juga kondisi masyarakat setempat yang tergolong mene ngah kebawah, sedangkan letak desa tersebut jauh dari lembaga pendidikan SMP dan setingkatnya yang ada disekitar desa tersebut. Penelitian dilakukan oleh Megadkk tahun 2020 di SD MI Sunan Ampel 1 Desa Sidorogo Kecamatan Taman Kabupaten di didapatkan hasil terdapat siswa sebanyak 44% terinfeksi STH, hal ini dikarenakan siswa yang kurang pengetahuan tentang kecacingan sebanyak 19 siswa (76%), sedangkan cukupnya pengetahuan tentang kecacingan sebanyak 4 siswa (16%), dan baiknya pengetahuan tentang kecacingan sebanyak 2 siswa (8%). dengan jumlah keseluruhan sebanyak 25 anak (100%). sebagian besar siswa maupun orang tua belum mengenal jenis-jenis cacing yang berbahaya bagi kesehatan, ataupun mengetahui cara pencegahan dan akibat kecacingan. Upaya yang dapat dilakukan adalah kegiatan penyuluhan yang berlangsu ng sangat interaktif dan komunikatif dan setiap peserta penyuluhan dapat memahami penjelasan yang disampaikan baik melalui media poster baik melalui tanya jawab. Berdasarkan hasil pretest dan post terdapat peningkatan pengetahun rata-rata 23% menjadi 71% ,sehingga total peningkatan pengetahuan peserta bertambah sebesar 48%.
The Implementation of Jigsaw Methods in Enhancing English Language Comprehension among Health Polytechnic Students Noer Amelia; Wisnu Istanto; Sulistyaning Edi-Krause; Sapto Dwi Anggoro; Merlissa Suemith
Anglophile Journal Vol 3 No 2 (2023): Anglophile Journal
Publisher : CV. Creative Tugu Pena

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51278/anglophile.v3i2.844

Abstract

The purpose of this research is to find out how much students at Health Polytechnic benefit from using Jigsaw techniques to better understand written English. In order to effectively communicate and understand medical principles and procedures, having a strong grasp of the English language is crucial in the healthcare industry. The study looked at how well 78 students from Poltekkes Kemenkes Surabaya learned using a Reading Proficiency test, traditional methods, and pre- and post-test experimental designs. It also used T-tests to look at the results. for a subset of the larger topic, which improves the group's ability to solve problems and make informed decisions. When students from different backgrounds have mastered a subject, they get together to share what they've learned and help each other out. The use of a group strategy not only leads to better overall comprehension, but also to the growth of interpersonal and communicative skills. The purpose of this research is to determine if teaching English using the Jigsaw method is effective for students at Health Polytechnics. The study will rely on statistical methods. Keywords: Jigsaw Method, Reading Proficiency, Cooperative Learning Strategy
Pemeriksaan Status Gizi dan Kadar Asam Urat Lansia di Posyandu Abiyoso Polkesbaya Evy Diah Woelansari; Juliana Christyaningsih; Suhariyadi Suhariyadi; Lully Hanni Endarini; Museyaroh Museyaroh; Diah Titik Mutiarawati; Wisnu Istanto; Suliati Suliati; Anik Handayati; Sri Sulami Endah Astuti; Pestariati Pestariati; Retno Sasongkowati; Mamik Mamik; Sri Utami; Minarti Minarti; Luthfi Rusyadi; Liliek Soetjiatie
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 2 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 2 Agustus 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i2.13417

Abstract

Proses penuaan dapat memengaruhi kesehatan fisik lansia dan meningkatkan risiko berbagai gangguan kesehatan, termasuk hipertensi dan asam urat. Kegiatan ini berlangsung pada tanggal 14 Juni 2023 bertempat di Balai RW 2 Kelurahan Kertajaya, Surabaya dan dihadiri 59 lansia yang berdomisili di RW 1 dan RW 2 Kelurahan Kertajaya Kecamatan Gubeng Surabaya. Jenis kegiatan yang dilaksanakan adalah pengukuran status gizi, asam urat dan senam hipertensi. Hasil pemeriksaan mengungkapkan 57,6% lansia memiliki kadar asam urat yang tinggi dan mayoritas memiliki kelebihan berat badan, sehingga lansia perlu mengendalikan tekanan darah dengan pengobatan yang diresepkan oleh dokter, perubahan gaya hidup seperti diet rendah garam, olahraga teratur, dan menghindari stres.
Pemanfaatan Kacang Kedelai (Glycine max (L.) Merill) Varietas Anjasmoro dan Dega-1 Sebagai Media Alternatif PDA (Potato Dextrose Agar) untuk Pertumbuhan Candida Albicans Putri, Ervina Wahyu; Sasongkowati, Retno; Astuti, Sri Sulami Endah; Istanto, Wisnu
Jurnal Penelitian Kesehatan SUARA FORIKES 2023
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf14nk123

Abstract

PDA (Potato Dextrose Agar) is a medium containing many nutrients needed by fungi, especially Candida albicans. Several researchers found alternative media for fungal growth using various carbohydrate sources such as cassava dextrose agar, canna tubers, yam tubers and arrowroot tubers on Candida albicans and Aspergillus niger. One of the components contained in PDA media is carbohydrates. Soybeans (Gllycine Max L. Merill) are a source of carbohydrates which are usually used as ingredients for food and beverage preparations (such as soy sauce, tempeh, tofu, and milk). Several soybean varieties that are included in the top 10 best quality with high nutritional content are the Anjasmoro variety and the Dega-1 variety. This study aimed to answer that the soybean (Glycine max (L.) Merill) varieties Anjasmoro and Dega-1 can be used as an alternative PDA medium for the growth of Candida albicans. The research method used was experimental to test the potential of the Anjasmoro variety and the Dega-1 variety as alternative media for PDA by analyzing the growth characteristics of Candida albicans with variations in the mass of soybean powder for each variety. The results showed that soybean variety Anjasmoro variety was better than soybean variety Dega-1 and has the potential to be an alternative media because the average number of colonies and morphology of Candida albicans at 4.4 gram mass variation was almost equivalent to the fungus growing on Gold media standard.Keywords: Candida albicans; Potato Dextrose Agar; soybean variety Anjasmoro; soybeans of the Dega-1 variety ABSTRAK PDA (Potato Dextrose Agar) merupakan media berisi banyak kandungan nutrisi yang dibutuhkan oleh jamur terutama Candida albicans. Beberapa peneliti menemukan media alternatif untuk pertumbuhan jamur menggunakan berbagai sumber karbohidrat seperti singkong dextrose agar, umbi ganyong, umbi gembili dan umbi garut pada Candida albicans dan Aspergillus niger. Salah satu komponen yang terdapat dalam media PDA yaitu karbohidrat. Kacang kedelai (Gllycine Max L. Merill) merupakan sumber karbohidrat yang biasanya dimanfaatkan sebagai bahan olahan makanan dan minuman (seperti kecap, tempe, tahu, dan susu). Beberapa varietas kacang kedelai yang termasuk 10 besar kualitas terbaik dengan kandungan gizi yang tinggi yaitu varietas Anjasmoro dan varietas Dega-1. Penelitian ini bertujuan untuk menjawab bahwa kacang kedelai (Glycine max (L.) Merill) varietas Anjasmoro dan Dega-1 dapat dimanfaatkan sebagai media alternatif PDA untuk pertumbuhan Candida albicans. Metode penelitian yang digunakan adalah eksperimental untuk menguji potensi kacang kedelai varietas Anjasmoro dan Varietas Dega-1 sebagai media alternatif PDA dengan menganalisis karakteristik pertumbuhan Candida albicans dengan variasi massa serbuk kacang kedelai masing-masing varietas. Hasil penelitian menunjukkan bahwa kacang kedelai varietas Anjasmoro lebih baik dibandingkan kacang kedelai varietas Dega-1 dan berpotensi untuk menjadi media alternatif dikarenakan pada rata-rata jumlah koloni dan morfologi Candida albicans pada variasi massa 4,4 gram hampir setara dengan jamur yang tumbuh pada media Gold standart.Kata kunci: Candida albicans; Potato Dextrose Agar; kacang kedelai varietas Anjasmoro; kacang kedelai varietas Dega-1
Difference in quality of preparates of mice kidney histology using xylol and sunflower oil (Helianthus annuus) on deparafinization processes Adin Bhagawanti Poernomo; Juliana Christyaningsih; Ratno Tri Utomo; Wisnu Istanto
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.512

Abstract

ematoxylin-Eosin staining begins with deparaffinization. Deparaffinization aims to remove the remaining paraffin in the tissue and xylol is a commonly used deparaffinization material but the use of xylol has a bad impact on health and also the environment because of its toxic nature besides the price of xylol is relatively more expensive. A more natural material that is not harmful to the body and the environment is needed as an alternative to xylol. Sunflower oil is a type of vegetable oil that contains oleic acid. The oleic acid content in sunflower oil has a chain of carbon atoms that is non-polar and water insoluble so that it can dissolve paraffin which is non-polar and hydrophobic. This study was conducted at Faculty of Veterinary Medicine Airlangga University, Veterinary Farm Center and the Cytohistotechnology Laboratory of the Medical Laboratory Technology Department of the Surabaya Ministry of Health Polytechnic also this study was conducted from January - May 2025. which examined the differences in the quality of mice kidney histology preparations using xylol and sunflower oil in the deparaffinization process with experimental research methods and the sample size obtained using the federer formula was 24 preparations and divided into 4 groups including xylol, sunflower oil at 50oC for 5 minutes, 10 minutes and 15 minutes. The data were statistically processed with the Kruskal Wallis non-parametric test and the results showed that Ho was accepted (P = 0.350) so it can be concluded that there is no significant difference between the preparations deparafinitation with sunflower oil and xylol in hematoxylin eosin
Co-Authors ., Pestariati Adin Bhagawanti Poernomo Anita Dwi Anggraini Diah Titik Mutiarawati Endah Astuti, Sri Sulami Endarini, Lully Hanni Evy Diah Woelansari Handayati, Anik Juliana Christyaningsih Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Merlissa Suemith Minarti Minarti Museyaroh, Museyaroh Noer Amelia Putri, Ervina Wahyu Ratno Tri Utomo Retno Sasongkowati Revi Zakiyatul Maftukhah Sapto Dwi Anggoro, Sapto Dwi Sasongkowati, Retno Sasongkowati, Retno Sri Utami Suhariyadi Suhariyadi Suliati Suliati Sulistyaning Edi-Krause